Title:
SCREENING AND ENGINEERING METHOD OF SUPER-STABLE IMMUNOGLOBULIN VARIABLE DOMAINS AND THEIR USES
Document Type and Number:
WIPO Patent Application WO/2014/030780
Kind Code:
A1
Abstract:
There are provided a method named Tat-associated protein engineering (TAPE), of screening a target protein having higher solubility and excellent thermostability, in particular, an immunoglobulin variable domain (VH or VL) derived from human germ cells, by preparing a gene construct where the target protein and an antibiotic -resistant protein are linked to a Tat signal sequence, and then expressing this within E. coli, and human or engineered VH and VL domain antibodies and human or engineered VH and VL domain antibody scaffolds having solubility and excellent thermostability, which are screened by the TAPE method. There are also provided a library including random CDR sequences in the human or engineered VH or VL domain antibody scaffold screened by the TAPE method, and a preparing method thereof. There are also provided a VH or VL domain antibody having binding ability to the target protein screened by using the library, and a pharmaceutical composition including the domain antibody.
More Like This:
Inventors:
LIM HYUNG-KWON (KR)
KIM SUNG GEUN (KR)
PARK YOUNG SEOUB (KR)
NAM HYO JUNG (KR)
KIM DONG-SIK (KR)
PARK JAE CHAN (KR)
YOON YEUP (KR)
KIM SUNG GEUN (KR)
PARK YOUNG SEOUB (KR)
NAM HYO JUNG (KR)
KIM DONG-SIK (KR)
PARK JAE CHAN (KR)
YOON YEUP (KR)
Application Number:
PCT/KR2012/006680
Publication Date:
February 27, 2014
Filing Date:
August 22, 2012
Export Citation:
Assignee:
MOGAM BIOTECH RES INST (KR)
LIM HYUNG-KWON (KR)
KIM SUNG GEUN (KR)
PARK YOUNG SEOUB (KR)
NAM HYO JUNG (KR)
KIM DONG-SIK (KR)
PARK JAE CHAN (KR)
YOON YEUP (KR)
LIM HYUNG-KWON (KR)
KIM SUNG GEUN (KR)
PARK YOUNG SEOUB (KR)
NAM HYO JUNG (KR)
KIM DONG-SIK (KR)
PARK JAE CHAN (KR)
YOON YEUP (KR)
International Classes:
C12N15/62; C07K16/28; C07K19/00; C12N15/13; C12N15/63; G01N33/53
Domestic Patent References:
| WO2007016591A2 | 2007-02-08 | |||
| WO2000034308A2 | 2000-06-15 | |||
| WO2007024877A2 | 2007-03-01 |
Foreign References:
| US20100285567A1 | 2010-11-11 | |||
| US20020012909A1 | 2002-01-31 | |||
| US20090011995A1 | 2009-01-08 | |||
| KR960007784B1 | 1996-06-12 |
Other References:
BETTER ET AL., SCIENCE, vol. 240, no. 4855, 1988, pages 1041 - 3
HUSTON ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 85, no. 16, 1988, pages 5879 - 83
BIRD ET AL., SCIENCE, vol. 242, no. 4877, 1988, pages 423 - 6
PEI ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 94, no. 18, 1997, pages 9637 - 42
ILIADES ET AL., FEBS LETT., vol. 409, no. 3, 1997, pages 437 - 41
WARD ET AL., NATURE, vol. 341, no. 6242, 1989, pages 544 - 6
ZHAO ET AL., BLOOD, vol. 110, no. 7, 2007, pages 2569 - 77
HOLLIGER ET AL., NAT, BIOTECHNOL., vol. 23, no. 9, 2005, pages 1126 - 36
HUDSON ET AL., MED. MICROBIOL IMMUNOL., vol. 198, no. 3, 2009, pages 157 - 74
ENEVER ET AL., CURR. OPIN. BIOTECHNOL., vol. 20, no. 4, 2009, pages 405 - 11
ELS ET AL., J BIOL CHEM., vol. 276, no. 10, 9 December 2000 (2000-12-09), pages 7346 - 50
BARGOU ET AL., SCIENCE, vol. 321, no. 5891, 2008, pages 974 - 7
MOL. CANCER THER., vol. 7, no. 8, 2008, pages 2288 - 97
CHOTHIA ET AL., J. MOL. BIOL., vol. 227, 1992, pages 799 - 917
TOMLINSON ET AL., EMBO J., vol. 14, 1995, pages 4628 - 4638
WILLIAMS ET AL., J. MOL. BIOL., vol. 264, pages 220 - 232
RUUD ET AL., J. MOL. BIOL., vol. 285, 1999, pages 895 - 901
J. MOL. RECOGNI., vol. 13, 2000, pages 167 - 187
HENDERSHOT ET AL., J. CELL BIOL., vol. 104, no. 3, 1987, pages 761 - 7
PRELLI ET AL., J. IMMUNOL., vol. 148, no. 3, 1992, pages 949 - 52
KABAT ET AL., J. IMMUNOL., vol. 147, no. 5, 1991, pages 1709 - 1719
COPPIETERS ET AL., ARTHRITIS RHEUM., vol. 54, no. 6, 2006, pages 1856 - 66
DOLK ET AL., PROTEINS, vol. 59, no. 3, 2005, pages 555 - 64
EWERT ET AL., BIOCHEM., vol. 41, no. 11, 2002, pages 3628 - 36
KORTT ET AL., J. PROTEIN CHEM., vol. 14, no. 3, 1995, pages 167 - 78
MARTIN ET AL., PROTEIN ENG., vol. 10, no. 5, 1997, pages 607 - 14
DAVIES ET AL., FEBS LETT., vol. 339, no. 3, 21 February 1994 (1994-02-21), pages 285 - 90
AIRES ET AL., J. MOL. BIOL., vol. 340, no. 3, 2004, pages 525 - 42
RIECHMANN E, J. MOL. BIOL., vol. 259, no. 5, 1996, pages 957 - 69
JOOST ET AL., DRUG DISCOVERY TODAY: TECHNOLOGIES, vol. 7, no. 2, 2010, pages 139 - 146
BARTHELEMY ET AL., J BIOL. CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
JESPERS ET AL., NAT. BIOTECHNOL., vol. 22, no. 9, 2004, pages 1161 - 5
BARTHELEMY ET AL., J BIOL CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
BANEYX; MUJACIC, NATURE BIOTECH, vol. 22, 2004, pages 1399 1408
TO ET AL., J. BIOL. CHEM., vol. 280, no. 50, 2005, pages 41395 - 403
MAXWELL ET AL., PROTEIN SCI., vol. 8, no. 9, 1999, pages 1908 - 11
WIGLEY ET AL., NAT. BIOTECHNOL., vol. 19, no. 2, 2001, pages 131 - 6
CABANTOUS ET AL., NAT BIOTECHNOL., vol. 23, no. 1, 2005, pages 102 - 7
WALDO GS, CURR. OPIN. CHEM. BIOL., vol. 7 1, 2003, pages 33 - 8
FISHER ET AL., PROTEIN SCI., vol. 15, no. 3, 2006, pages 449 - 58
LIM ET AL., PROTEIN SCI., vol. 18, no. 12, 2009, pages 2537 - 49
FISHER AC; DELISA MP, J MOL BIOL., vol. 385, no. 1, 2009, pages 299 - 311
SANDERS ET AL., MOL. MICROBIOL., vol. 41, no. 1, 2001, pages 241 - 6
DELISA ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 100, no. 10, 2003, pages 6115 - 20
MATOS ET AL., EMBO J., vol. 27, no. 15, 2008, pages 2055 - 63
FISHER AC; DELISA MP, J. MOL. BIOL., vol. 385, no. 1, 2009, pages 299 - 311
LIM ET AL., PROTEIN SCI, vol. 18, no. 12, 2009, pages 2537 - 49
KRISTENSEN P; WINTER G, FOLD. DES., vol. 3, no. 5, 1998, pages 321 - 8
SIEBER ET AL., NAT. BIOTECHNOL., vol. 16, no. 10, 1998, pages 955 - 60
JUNG ET AL., J. MOL. BIOL., vol. 294, no. 1, 1999, pages 163 - 80
WORN A; PLÜCKTHUN A, J. MOL. BIOL., vol. 305, no. 5, 2001, pages 989 - 1010
CHOTHIA ET AL., J MOL BIOL, vol. 227, 1992, pages 799 - 917
TOMLINSON ET AL., EMBO J, vol. 14, 1995, pages 4628 - 4638
WILLIAMS ET AL., J MOL BIOL, vol. 264, pages 220 - 232
BANEYX; MUJACIC, NAT. BIOTECH., vol. 22, 2004, pages 1399 - 1408
DELISA ET AL., PNAS, vol. 100, no. 10, 2003, pages 6115 - 6120
SNADERS ET AL., MOL MICROBIOL, vol. 41, no. 1, 2001, pages 241 - 246
MATOS ET AL., EMBO J, vol. 27, no. 15, 2008, pages 2055 - 2063
FISHER ET AL., PROTEIN SCI, vol. 15, no. 3, 2006, pages 449 - 458
LIM ET AL., PROTEIN SCI, vol. 18, no. 12, 2009, pages 2537 - 2549
JEPSERS L. ET AL., NAT. BIOTECHNOL., vol. 22, no. 9, 2004, pages 1161 - 5
BARTHELEMY P. A. ET AL., J. BIOL. CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
J. BIOL. CHEM., vol. 284, no. 21, 22 May 2009 (2009-05-22), pages 14203 - 14210
FISHER A. C. ET AL., PROTEIN SCI., vol. 15, no. 3, March 2006 (2006-03-01), pages 449 - 58
FISHER A. C. ET AL., J. MOL. BIOL., vol. 385, no. 1, 2009, pages 299 - 311
PÉDELACQ ET AL., NAT BIOTECHNOL., vol. 20, no. 9, 2002, pages 927 - 32
YANG ET AL., PROC. NATL. ACAD. SCI., vol. 100, no. 2, 2003, pages 455 - 60
LIM HK ET AL.: "Production Characteristics of Interferon-a Using an L-arabinose Promoter System in a High-cell-density Culture", APPL. MICROBIOL. BIOTECHNOL., vol. 53, no. 2, pages 201 - 208
STEFAN EWERT ET AL.: "Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering", METHODS, vol. 34, 2004, pages 184 - 199, XP004526805, DOI: doi:10.1016/j.ymeth.2004.04.007
EWERT ET AL.: "Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering", METHODS, vol. 34, 2004, pages 184 - 199, XP004526805, DOI: doi:10.1016/j.ymeth.2004.04.007
CHRISTOPER J BOND. ET AL., J. MOL. BIOL., vol. 348, 2005, pages 699 - 709
AKIKO KOIDE ET AL., PNAS, vol. 104, no. 16, 2007, pages 6632 - 6637
SMITH ET AL., SCIENCE, vol. 228, no. 4705, 1985, pages 1315 - 1317
CARLOS F. BARBAS III ET AL.: "Phage Display - A Laboratory Manual", COLD SPRING HARBOR LABORATORY PRESS
GINGER CHAO ET AL., NATURE PROTOCOL, vol. 1, no. 2, 2006, pages 755 - 768
MINGYUE H. ET AL., NATURE PROTOCOL, vol. 4, no. 3, 2007, pages 281 - 288
See also references of EP 2888367A4
HUSTON ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 85, no. 16, 1988, pages 5879 - 83
BIRD ET AL., SCIENCE, vol. 242, no. 4877, 1988, pages 423 - 6
PEI ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 94, no. 18, 1997, pages 9637 - 42
ILIADES ET AL., FEBS LETT., vol. 409, no. 3, 1997, pages 437 - 41
WARD ET AL., NATURE, vol. 341, no. 6242, 1989, pages 544 - 6
ZHAO ET AL., BLOOD, vol. 110, no. 7, 2007, pages 2569 - 77
HOLLIGER ET AL., NAT, BIOTECHNOL., vol. 23, no. 9, 2005, pages 1126 - 36
HUDSON ET AL., MED. MICROBIOL IMMUNOL., vol. 198, no. 3, 2009, pages 157 - 74
ENEVER ET AL., CURR. OPIN. BIOTECHNOL., vol. 20, no. 4, 2009, pages 405 - 11
ELS ET AL., J BIOL CHEM., vol. 276, no. 10, 9 December 2000 (2000-12-09), pages 7346 - 50
BARGOU ET AL., SCIENCE, vol. 321, no. 5891, 2008, pages 974 - 7
MOL. CANCER THER., vol. 7, no. 8, 2008, pages 2288 - 97
CHOTHIA ET AL., J. MOL. BIOL., vol. 227, 1992, pages 799 - 917
TOMLINSON ET AL., EMBO J., vol. 14, 1995, pages 4628 - 4638
WILLIAMS ET AL., J. MOL. BIOL., vol. 264, pages 220 - 232
RUUD ET AL., J. MOL. BIOL., vol. 285, 1999, pages 895 - 901
J. MOL. RECOGNI., vol. 13, 2000, pages 167 - 187
HENDERSHOT ET AL., J. CELL BIOL., vol. 104, no. 3, 1987, pages 761 - 7
PRELLI ET AL., J. IMMUNOL., vol. 148, no. 3, 1992, pages 949 - 52
KABAT ET AL., J. IMMUNOL., vol. 147, no. 5, 1991, pages 1709 - 1719
COPPIETERS ET AL., ARTHRITIS RHEUM., vol. 54, no. 6, 2006, pages 1856 - 66
DOLK ET AL., PROTEINS, vol. 59, no. 3, 2005, pages 555 - 64
EWERT ET AL., BIOCHEM., vol. 41, no. 11, 2002, pages 3628 - 36
KORTT ET AL., J. PROTEIN CHEM., vol. 14, no. 3, 1995, pages 167 - 78
MARTIN ET AL., PROTEIN ENG., vol. 10, no. 5, 1997, pages 607 - 14
DAVIES ET AL., FEBS LETT., vol. 339, no. 3, 21 February 1994 (1994-02-21), pages 285 - 90
AIRES ET AL., J. MOL. BIOL., vol. 340, no. 3, 2004, pages 525 - 42
RIECHMANN E, J. MOL. BIOL., vol. 259, no. 5, 1996, pages 957 - 69
JOOST ET AL., DRUG DISCOVERY TODAY: TECHNOLOGIES, vol. 7, no. 2, 2010, pages 139 - 146
BARTHELEMY ET AL., J BIOL. CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
JESPERS ET AL., NAT. BIOTECHNOL., vol. 22, no. 9, 2004, pages 1161 - 5
BARTHELEMY ET AL., J BIOL CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
BANEYX; MUJACIC, NATURE BIOTECH, vol. 22, 2004, pages 1399 1408
TO ET AL., J. BIOL. CHEM., vol. 280, no. 50, 2005, pages 41395 - 403
MAXWELL ET AL., PROTEIN SCI., vol. 8, no. 9, 1999, pages 1908 - 11
WIGLEY ET AL., NAT. BIOTECHNOL., vol. 19, no. 2, 2001, pages 131 - 6
CABANTOUS ET AL., NAT BIOTECHNOL., vol. 23, no. 1, 2005, pages 102 - 7
WALDO GS, CURR. OPIN. CHEM. BIOL., vol. 7 1, 2003, pages 33 - 8
FISHER ET AL., PROTEIN SCI., vol. 15, no. 3, 2006, pages 449 - 58
LIM ET AL., PROTEIN SCI., vol. 18, no. 12, 2009, pages 2537 - 49
FISHER AC; DELISA MP, J MOL BIOL., vol. 385, no. 1, 2009, pages 299 - 311
SANDERS ET AL., MOL. MICROBIOL., vol. 41, no. 1, 2001, pages 241 - 6
DELISA ET AL., PROC. NATL. ACAD. SCI. U S A., vol. 100, no. 10, 2003, pages 6115 - 20
MATOS ET AL., EMBO J., vol. 27, no. 15, 2008, pages 2055 - 63
FISHER AC; DELISA MP, J. MOL. BIOL., vol. 385, no. 1, 2009, pages 299 - 311
LIM ET AL., PROTEIN SCI, vol. 18, no. 12, 2009, pages 2537 - 49
KRISTENSEN P; WINTER G, FOLD. DES., vol. 3, no. 5, 1998, pages 321 - 8
SIEBER ET AL., NAT. BIOTECHNOL., vol. 16, no. 10, 1998, pages 955 - 60
JUNG ET AL., J. MOL. BIOL., vol. 294, no. 1, 1999, pages 163 - 80
WORN A; PLÜCKTHUN A, J. MOL. BIOL., vol. 305, no. 5, 2001, pages 989 - 1010
CHOTHIA ET AL., J MOL BIOL, vol. 227, 1992, pages 799 - 917
TOMLINSON ET AL., EMBO J, vol. 14, 1995, pages 4628 - 4638
WILLIAMS ET AL., J MOL BIOL, vol. 264, pages 220 - 232
BANEYX; MUJACIC, NAT. BIOTECH., vol. 22, 2004, pages 1399 - 1408
DELISA ET AL., PNAS, vol. 100, no. 10, 2003, pages 6115 - 6120
SNADERS ET AL., MOL MICROBIOL, vol. 41, no. 1, 2001, pages 241 - 246
MATOS ET AL., EMBO J, vol. 27, no. 15, 2008, pages 2055 - 2063
FISHER ET AL., PROTEIN SCI, vol. 15, no. 3, 2006, pages 449 - 458
LIM ET AL., PROTEIN SCI, vol. 18, no. 12, 2009, pages 2537 - 2549
JEPSERS L. ET AL., NAT. BIOTECHNOL., vol. 22, no. 9, 2004, pages 1161 - 5
BARTHELEMY P. A. ET AL., J. BIOL. CHEM., vol. 283, no. 6, 2008, pages 3639 - 54
J. BIOL. CHEM., vol. 284, no. 21, 22 May 2009 (2009-05-22), pages 14203 - 14210
FISHER A. C. ET AL., PROTEIN SCI., vol. 15, no. 3, March 2006 (2006-03-01), pages 449 - 58
FISHER A. C. ET AL., J. MOL. BIOL., vol. 385, no. 1, 2009, pages 299 - 311
PÉDELACQ ET AL., NAT BIOTECHNOL., vol. 20, no. 9, 2002, pages 927 - 32
YANG ET AL., PROC. NATL. ACAD. SCI., vol. 100, no. 2, 2003, pages 455 - 60
LIM HK ET AL.: "Production Characteristics of Interferon-a Using an L-arabinose Promoter System in a High-cell-density Culture", APPL. MICROBIOL. BIOTECHNOL., vol. 53, no. 2, pages 201 - 208
STEFAN EWERT ET AL.: "Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering", METHODS, vol. 34, 2004, pages 184 - 199, XP004526805, DOI: doi:10.1016/j.ymeth.2004.04.007
EWERT ET AL.: "Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering", METHODS, vol. 34, 2004, pages 184 - 199, XP004526805, DOI: doi:10.1016/j.ymeth.2004.04.007
CHRISTOPER J BOND. ET AL., J. MOL. BIOL., vol. 348, 2005, pages 699 - 709
AKIKO KOIDE ET AL., PNAS, vol. 104, no. 16, 2007, pages 6632 - 6637
SMITH ET AL., SCIENCE, vol. 228, no. 4705, 1985, pages 1315 - 1317
CARLOS F. BARBAS III ET AL.: "Phage Display - A Laboratory Manual", COLD SPRING HARBOR LABORATORY PRESS
GINGER CHAO ET AL., NATURE PROTOCOL, vol. 1, no. 2, 2006, pages 755 - 768
MINGYUE H. ET AL., NATURE PROTOCOL, vol. 4, no. 3, 2007, pages 281 - 288
See also references of EP 2888367A4
Attorney, Agent or Firm:
KWON, Oh-Sig et al. (138 Dunsanjung-ro Seo-gu, Daejeon 302-120, KR)
Download PDF: