Title:
SCREENING AND USE OF BIOMARKER RELATED TO SEVERE OLIGOASTHENOSPERMIA
Document Type and Number:
WIPO Patent Application WO/2018/176808
Kind Code:
A1
Abstract:
Provided is a method for screening a biomarker related to severe oligoasthenospermia. The method comprises firstly carrying out mass spectrometry on non-coding amino acids of multiple groups of sperm proteins in the case of severe oligoasthenospermia by means of a NanoHPLC-MS/MS mass spectrometry system and an non-labelled quantitative proteomics method; then searching for the mass spectrometry data by means of a non-limiting amino acid protein modification analysis method, and carrying out multivariable Gaussian mixture distribution clustering analysis to identify the non-coding amino acids in the sperm protein groups to the greatest possible extent; and finally, comparing the non-coding amino acids of normal and patient sperm protein groups to obtain non-coding amino acid sites of the protein related to severe oligoasthenospermia, wherein same are thereby used as molecular markers of severe oligoasthenospermia. The method provides new diagnostic and therapeutic targets for severe oligoasthenospermia.
Inventors:
YANG JINGHUA (CN)
CHEN XINJUN (CN)
LV XIN (CN)
ZHAO HAN (CN)
LI CUILING (CN)
BI BAIBIN (CN)
GONG JING (CN)
WANG FENGQIN (CN)
SUN SHENGNAN (CN)
WANG XINGYUAN (CN)
CHEN ZIJIANG (CN)
YANG JINGMING (CN)
CHEN XINJUN (CN)
LV XIN (CN)
ZHAO HAN (CN)
LI CUILING (CN)
BI BAIBIN (CN)
GONG JING (CN)
WANG FENGQIN (CN)
SUN SHENGNAN (CN)
WANG XINGYUAN (CN)
CHEN ZIJIANG (CN)
YANG JINGMING (CN)
Application Number:
PCT/CN2017/106915
Publication Date:
October 04, 2018
Filing Date:
October 19, 2017
Export Citation:
Assignee:
UNIV SHANDONG (CN)
International Classes:
G01N30/88
Foreign References:
| CN106872630A | 2017-06-20 | |||
| CN107024553A | 2017-08-08 | |||
| CN106932597A | 2017-07-07 | |||
| CN106996979A | 2017-08-01 | |||
| CN107015005A | 2017-08-04 | |||
| CN107037172A | 2017-08-11 | |||
| CN106996981A | 2017-08-01 | |||
| CN106996980A | 2017-08-01 | |||
| CN106990177A | 2017-07-28 | |||
| CN101477089A | 2009-07-08 |
Other References:
PARTE, P.P.: "Sperm phosphoproteome profiling by ultra performance liquid chromatography followed by data independent analysis (LC - MSE) reveals alte- red proteomic signatures in asthenozoospermia", JOURNAL OF PROTEOMICS, vol. 75, no. 18, 13 July 2012 (2012-07-13), pages 5861 - 5871, XP055611860, DOI: 10.1016/j.jprot.2012.07.003
MCDONALD, W.H.: "Shotgun proteomics and biomarker discovery", DISEASE MARKERS, vol. 18, no. 2, 31 December 2002 (2002-12-31), pages 99 - 105, XP055611870, DOI: 10.1155/2002/505397
CHAN, C. C .: "Motility and Protein Phosphorylation in Healthy and Asthenozoospermic Sperm", JOURNAL OF PROTEOME RESEARCH, vol. 8, no. 11, 13 August 2009 (2009-08-13), pages 5382 - 5386, XP055611871, DOI: 10.1021/pr9003932
XIA, XIYANG: "Proteomic analysis of novel post-translational modifications in human and mouse testes", MEDICINE & PUBLIC HEALTH, CHINA MASTER'S THESES FULL-TEXT DATABASE, no. 4, 15 April 2016 (2016-04-15), pages E059-34
MCDONALD, W.H.: "Shotgun proteomics and biomarker discovery", DISEASE MARKERS, vol. 18, no. 2, 31 December 2002 (2002-12-31), pages 99 - 105, XP055611870, DOI: 10.1155/2002/505397
CHAN, C. C .: "Motility and Protein Phosphorylation in Healthy and Asthenozoospermic Sperm", JOURNAL OF PROTEOME RESEARCH, vol. 8, no. 11, 13 August 2009 (2009-08-13), pages 5382 - 5386, XP055611871, DOI: 10.1021/pr9003932
XIA, XIYANG: "Proteomic analysis of novel post-translational modifications in human and mouse testes", MEDICINE & PUBLIC HEALTH, CHINA MASTER'S THESES FULL-TEXT DATABASE, no. 4, 15 April 2016 (2016-04-15), pages E059-34
Attorney, Agent or Firm:
JINAN SHENGDA INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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