Title:
SINGLE CELL FACTORY EFFICIENTLY SYNTHESIZING α-AMINOBUTYRIC ACID, AND CONSTRUCTION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/090288
Kind Code:
A1
Abstract:
Provided is an engineering bacteria synthesizing α-aminobutyric acid, wherein the preparation method is: after the tandem of L-threonine deaminase, L-amino acid dehydrogenase and a dehydrogenase gene for providing cofactor NADH circulation, constructing a co-expression vector, and then transfecting same into host bacteria. The vector comprises a promoter and a ribosome binding site sequence designed respectively, so as to control the three enzymes and realize the coordinated expression, and increase the final yield of α-aminobutyric acid.
Inventors:
RAO ZHIMING (CN)
ZHOU JUNPING (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
XU MEIJUAN (CN)
ZHANG CAIZHE (CN)
QI YUNLONG (CN)
ZHENG JUNXIAN (CN)
ZHOU JUNPING (CN)
YANG TAOWEI (CN)
ZHANG XIAN (CN)
XU MEIJUAN (CN)
ZHANG CAIZHE (CN)
QI YUNLONG (CN)
ZHENG JUNXIAN (CN)
Application Number:
PCT/CN2016/106241
Publication Date:
May 24, 2018
Filing Date:
November 17, 2016
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12P7/40; C12P13/04; C12R1/19
Foreign References:
| CN102212567A | 2011-10-12 | |||
| CN105238807A | 2016-01-13 | |||
| CN105255934A | 2016-01-20 | |||
| CN102517351A | 2012-06-27 | |||
| CN104774881A | 2015-07-15 |
Other References:
YIN XIANQING ET AL.: "Development of synthesis of alpha-Aminobutyric Acid", FINE CHEMICAL INTERMEDIATES, vol. 40, no. 1, 28 February 2010 (2010-02-28), pages 12 - 14
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO.,LTD. (CN)
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