Title:
SOLUBLE EXPRESSION OF PROTEIN USING PEPTIDE TAG
Document Type and Number:
WIPO Patent Application WO/2020/045530
Kind Code:
A1
Abstract:
In the present invention, by connecting a peptide tag having the amino acid sequence below to a target protein and causing the target protein to be expressed, the target protein is efficiently accumulated in a soluble fraction. X(PY)qPZ, where P represents proline, X represents an amino acid sequence comprising 0-5 amino acids independently selected from the group consisting of arginine (R), glycine (G), serine (S), lysine (K), threonine (T), leucine (L), asparagine (N), glutamine (Q), and methionine (M), Y represents an amino acid sequence comprising 1-4 amino acids independently selected from the group consisting of R, G, K, T, L, N, Q, and M, q is an integer of 1-10, Z represents an amino acid sequence comprising 0-10 amino acids independently selected from the group consisting of R, G, S, K, T, L, N, Q, and M, and a total of three or more of Q, M, L, N, and T are included in the amino acid sequence of the peptide tag.
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Inventors:
KOIKE KAZUYOSHI (JP)
TAKITA EIJI (JP)
MATSUI TAKESHI (JP)
TAKITA EIJI (JP)
MATSUI TAKESHI (JP)
Application Number:
PCT/JP2019/033792
Publication Date:
March 05, 2020
Filing Date:
August 28, 2019
Export Citation:
Assignee:
IDEMITSU KOSAN CO (JP)
International Classes:
C12N15/62; C07K14/00; C07K19/00; C12N15/64; C12P21/02; C07K1/13
Domestic Patent References:
| WO2017115853A1 | 2017-07-06 | |||
| WO2019167962A1 | 2019-09-06 | |||
| WO2017217460A1 | 2017-12-21 | |||
| WO2009133882A1 | 2009-11-05 | |||
| WO2017115853A1 | 2017-07-06 |
Foreign References:
| US20090137004A1 | 2009-05-28 |
Other References:
KANG ET AL., PROTEIN EXPR PURIF., vol. 38, no. 1, November 2004 (2004-11-01), pages 129 - 35
TADA ET AL., THEOR. APPL. GENET, vol. 80, 1990, pages 475
GENE, vol. 39, 1985, pages 281 - 286
LAZZERI ET AL., THEOR. APPL. GENET., vol. 81, 1991, pages 437
HOOD ET AL., TRANSGENIC, RES., vol. 2, 1993, pages 218
HIEI ET AL., PLANT J., vol. 6, 1994, pages 271
SANFORD ET AL., J. PART. SCI. TECH., vol. 5, 1987, pages 27
OHTSUK ET AL., FEBS LETT., vol. 428, no. 3, 29 May 1998 (1998-05-29), pages 235 - 40
See also references of EP 3845653A4
TADA ET AL., THEOR. APPL. GENET, vol. 80, 1990, pages 475
GENE, vol. 39, 1985, pages 281 - 286
LAZZERI ET AL., THEOR. APPL. GENET., vol. 81, 1991, pages 437
HOOD ET AL., TRANSGENIC, RES., vol. 2, 1993, pages 218
HIEI ET AL., PLANT J., vol. 6, 1994, pages 271
SANFORD ET AL., J. PART. SCI. TECH., vol. 5, 1987, pages 27
OHTSUK ET AL., FEBS LETT., vol. 428, no. 3, 29 May 1998 (1998-05-29), pages 235 - 40
See also references of EP 3845653A4
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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