SPECIFIC DETECTION AND QUANTIFICATION OF CARDIOLIPIN AND ISOLATED MITOCHONDRIA BY POSITIVELY CHARGED AIE FLUOROGENS AND METHOD OF MANUFACTURING THEREOF

Title:

SPECIFIC DETECTION AND QUANTIFICATION OF CARDIOLIPIN AND ISOLATED MITOCHONDRIA BY POSITIVELY CHARGED AIE FLUOROGENS AND METHOD OF MANUFACTURING THEREOF

Document Type and Number:

WIPO Patent Application WO/2015/081860

Kind Code:

A1

Abstract:

The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

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Inventors:

TANG BENZHONG (CN)
HONG YUNING (CN)
LEUNG WAI TUNG (CN)

Application Number:

PCT/CN2014/092898

Publication Date:

June 11, 2015

Filing Date:

December 03, 2014

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Assignee:

UNIV HONG KONG SCIENCE & TECHN (CN)

International Classes:

G01N21/64; C09K11/06

Domestic Patent References:

WO2013029340A1

2013-03-07

Foreign References:

US20120172296A1	2012-07-05
CN101679849A	2010-03-24
CN101243321A	2008-08-13
CN102702096A	2012-10-03

Attorney, Agent or Firm:

TEE & HOWE INTELLECTUAL PROPERTY ATTORNEYS (10th Floor Tower D,Minsheng Financial Center, 28 Jianguomennei Avenu, Dongcheng District Beijing 5, CN)

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