SPECIFIC NANOANTIBODY NB3.27 AGAINST COLORECTAL CANCER-ASSOCIATED BACTEROIDES FRAGILIS TOXIN PROTEIN ACTIVATOR, AND APPLICATION THEREOF

Title:

SPECIFIC NANOANTIBODY NB3.27 AGAINST COLORECTAL CANCER-ASSOCIATED BACTEROIDES FRAGILIS TOXIN PROTEIN ACTIVATOR, AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2023/137994

Kind Code:

A1

Abstract:

Disclosed in the present invention are a specific nanoantibody Nb3.27 against a colorectal cancer-associated Bacteroides fragilis toxin protein activator, and an application thereof. By means of in-depth research of enterotoxigenic Bacteroides fragilis in tumor patients and an activator of Bacteroides fragilis toxin protein (BFT-211) secreted by the enterotoxigenic Bacteroides fragilis, a BFT-211 nanoantibody is expressed by means of phage display technology; a nanoantibody having a relatively high binding force to antigen is selected by means of biopanning technology; a BFT-211 nanoantibody, which is also called Nb3.27, is obtained. In the present invention, a stable and uniform BFT-211 nanoantibody protein having high affinity is obtained by means of expression and optimization, a new field of early diagnosis of colorectal cancer and breast cancer by the nanoantibody targeting BFT-211 is opened, and the present invention has a good research value and application prospect.

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Inventors:

WEN YURONG (CN)
ZHENG FANG (CN)
GUO YUCHENG (CN)

Application Number:

PCT/CN2022/103030

Publication Date:

July 27, 2023

Filing Date:

June 30, 2022

Export Citation:

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Assignee:

SHAANXI HAISINUOWEI TECH CO LTD (CN)

International Classes:

C07K1/14; C07K16/12; C12N1/21; C12N15/70; G01N33/68

Domestic Patent References:

WO2020237023A1	2020-11-26
WO1987006616A1	1987-11-05

Foreign References:

CN114409777A	2022-04-29
CN112739376A	2021-04-30

Other References:

CHENG WAI TENG, KANTILAL HARESH KUMAR, DAVAMANI FABIAN: "The Mechanism of Bacteroides fragilis Toxin Contributes to Colon Cancer Formation", MALAYSIAN JOURNAL OF MEDICAL SCIENCE, KERIAN, vol. 27, no. 4, 1 January 2020 (2020-01-01), pages 9 - 21, XP093080111, ISSN: 1394-195X, DOI: 10.21315/mjms2020.27.4.2

Attorney, Agent or Firm:

XI'AN TONG DA PATENT AGENCY CO., LTD. (CN)

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