Title:
SPECIMEN PRETREATMENT METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/157457
Kind Code:
A1
Abstract:
Conventional protein-containing specimen pretreatment methods preceding analysis using LC-MS or electrophoresis are time-consuming, which is a limitation for various applications of the methods. Said methods also have problems such as sample loss. Development of a rapid and highly efficient pretreatment method with which said problems can be solved was necessary. Provided is a pretreatment method for a specimen containing a protein, said method including: a step for simultaneously carrying out protein denaturation and protein digestion which uses a heat-resistant enzyme immobilized on a substrate; and a step for recovering digested peptides.
Inventors:
OFOSU-TWUM ERIC (JP)
MARUYAMA MASASHI (JP)
NOBUKI SHUNICHIROU (JP)
MARUYAMA MASASHI (JP)
NOBUKI SHUNICHIROU (JP)
Application Number:
PCT/JP2022/046771
Publication Date:
August 24, 2023
Filing Date:
December 20, 2022
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12P21/00; C07K1/04; C07K1/14; C12M1/00; C12P1/00; G01N30/06; G01N33/48; G01N33/68
Foreign References:
| US20150361415A1 | 2015-12-17 | |||
| JP2007511837A | 2007-05-10 |
Other References:
BARK, STEVEN J. ET AL.: "High-Temperature Protein Mass Mapping Using a Thermophilic Protease", J. AM. CHEM. SOC., vol. 123, 2001, pages 1774 - 1775, XP055417355, DOI: 10.1021/ja002909n
TODA, HIROSHI: "Use of immobilized enzymes and cells", SEIBUTSU KOGAKU KAISHI = JOURNAL OF THE SOCIETY FOR BIOSCIENCE AND BIOENGINEERING, JAPAN, NIPPON SEIBUTSU KOGAKKAI, SUITA,, JP, vol. 96, no. 8, JP , pages 467 - 471, XP009548694, ISSN: 0919-3758
TODA, HIROSHI: "Use of immobilized enzymes and cells", SEIBUTSU KOGAKU KAISHI = JOURNAL OF THE SOCIETY FOR BIOSCIENCE AND BIOENGINEERING, JAPAN, NIPPON SEIBUTSU KOGAKKAI, SUITA,, JP, vol. 96, no. 8, JP , pages 467 - 471, XP009548694, ISSN: 0919-3758
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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