Title:
SPHINGOMYELINASE
Document Type and Number:
WIPO Patent Application WO/2008/136451
Kind Code:
A1
Abstract:
Disclosed are a novel sphingomyelinase and a method for the production thereof.
The sphingomyelinase comprises: (a) a polypeptide comprising the amino acid
sequence depicted in SEQ ID NO:2; (b) a polypeptide which comprises an amino acid
sequence having the substitution, insertion, deletion and/or addition of one
or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:2
and which has the hydrolytic activity; or (c) a polypeptide which comprises an
amino acid sequence having an at least 75% homology with the amino acid sequence
depicted in SEQ ID NO:2 and which has the hydrolytic activity. The enzyme may have
a hydrolytic activity of 50% or more on sphingomyeline (a substrate) at a pH value
ranging from pH 8 to 12, wherein the hydrolytic activity of the enzyme on sphingomyeline
at pH 10 and 37°C for 10 minutes is defined as 100%.
Inventors:
YAMAGUCHI HITOMI (JP)
SAKODA AKIKO (JP)
SHIIHARA MISA (JP)
IWASAKI YUGO (JP)
SAKODA AKIKO (JP)
SHIIHARA MISA (JP)
IWASAKI YUGO (JP)
Application Number:
PCT/JP2008/058159
Publication Date:
November 13, 2008
Filing Date:
April 21, 2008
Export Citation:
Assignee:
NAGASE & CO LTD (JP)
NAGASE CHEMTEX CORP (JP)
UNIV NAGOYA NAT UNIV CORP (JP)
YAMAGUCHI HITOMI (JP)
SAKODA AKIKO (JP)
SHIIHARA MISA (JP)
IWASAKI YUGO (JP)
NAGASE CHEMTEX CORP (JP)
UNIV NAGOYA NAT UNIV CORP (JP)
YAMAGUCHI HITOMI (JP)
SAKODA AKIKO (JP)
SHIIHARA MISA (JP)
IWASAKI YUGO (JP)
International Classes:
C12N15/09; C12N1/21; C12N9/16; C12R1/465
Foreign References:
| JP2005523019A | 2005-08-04 |
Other References:
MATSUYAMA H. ET AL.: "Alteration in reactivity of sphingomyelinase from Streptomyces sp. modified with a polyethylene glycol", BIOL. PHARM. BULL., vol. 16, no. 2, 1993, pages 107 - 111
SUZUKI K. ET AL.: "Purification and some properties of S-Hemolysin produced by Streptomyces sp. strain no. A-6288", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, no. 11, 1995, pages 2081 - 2086
DATABASE GENPEPT [online] 17 April 2007 (2007-04-17), COPELAND A. ET AL.: "Sphingomyelin phosphodiesterase [Salinispora tropica CNB-440]", accession no. NCBI Database accession no. (ABP54498)
DATABASE GENBANK [online] 17 April 2007 (2007-04-17), COPELAND A. ET AL.: "Salinispora tropica CNB-440, complete genome", accession no. NCBI Database accession no. (CP000667)
DZIEWANOWSKA K.: "Comparison of the beta-toxins from Straphylococcus aureus and Staphylococcus intermedius", ARCH. BIOCHEM. BIOPHYS., vol. 335, no. 1, 1996, pages 102 - 108
PROJAN S.J. ET AL.: "Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus", NUCLEIC ACIDS RES., vol. 17, no. 8, 1989, pages 3305
YAMADA A.: "Nucleotide sequence and expression in Escherichia coli of the gene coding for sphingomyelinase of Bacillus cereus", EUR. J. BIOCHEM., vol. 175, 1988, pages 213 - 220
SEGERS R.P. ET AL.: "Molecular analysis of a sphingomyelinase C gene from Leptospira interrogans serovar hardjo", INFECT. IMMUN., vol. 58, no. 7, 1990, pages 2177 - 2185
GONZALEZ-ZORN B. ET AL.: "The smcL gene of Listeria ivanovii encodes as phingomyelinase C that mediates bacterial escape from the phagocytic vacuole", MOL. MICROBIOL., vol. 33, no. 3, 1999, pages 510 - 523
DATABASE GENPEPT [online] 16 July 2007 (2007-07-16), SUGIMORI D.: "Sphingomyelinase C [Streptomyces Griseocarneus]", accession no. NCBI Database accession no. (BAF73613)
SUZUKI K. ET AL.: "Purification and some properties of S-Hemolysin produced by Streptomyces sp. strain no. A-6288", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 59, no. 11, 1995, pages 2081 - 2086
DATABASE GENPEPT [online] 17 April 2007 (2007-04-17), COPELAND A. ET AL.: "Sphingomyelin phosphodiesterase [Salinispora tropica CNB-440]", accession no. NCBI Database accession no. (ABP54498)
DATABASE GENBANK [online] 17 April 2007 (2007-04-17), COPELAND A. ET AL.: "Salinispora tropica CNB-440, complete genome", accession no. NCBI Database accession no. (CP000667)
DZIEWANOWSKA K.: "Comparison of the beta-toxins from Straphylococcus aureus and Staphylococcus intermedius", ARCH. BIOCHEM. BIOPHYS., vol. 335, no. 1, 1996, pages 102 - 108
PROJAN S.J. ET AL.: "Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus", NUCLEIC ACIDS RES., vol. 17, no. 8, 1989, pages 3305
YAMADA A.: "Nucleotide sequence and expression in Escherichia coli of the gene coding for sphingomyelinase of Bacillus cereus", EUR. J. BIOCHEM., vol. 175, 1988, pages 213 - 220
SEGERS R.P. ET AL.: "Molecular analysis of a sphingomyelinase C gene from Leptospira interrogans serovar hardjo", INFECT. IMMUN., vol. 58, no. 7, 1990, pages 2177 - 2185
GONZALEZ-ZORN B. ET AL.: "The smcL gene of Listeria ivanovii encodes as phingomyelinase C that mediates bacterial escape from the phagocytic vacuole", MOL. MICROBIOL., vol. 33, no. 3, 1999, pages 510 - 523
DATABASE GENPEPT [online] 16 July 2007 (2007-07-16), SUGIMORI D.: "Sphingomyelinase C [Streptomyces Griseocarneus]", accession no. NCBI Database accession no. (BAF73613)
Attorney, Agent or Firm:
NANJO, Hiromichi (Okina Bldg. 2-9, Nishitenma 3-chome,Kita-k, Osaka-shi Osaka 47, JP)
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