Title:
STAINING METHOD FOR LIVE-CELL IMAGING
Document Type and Number:
WIPO Patent Application WO/2022/252104
Kind Code:
A1
Abstract:
A fluorescent staining method for live-cell imaging, comprising: performing double staining on cells by means of a first fluorescent biomarker and a second fluorescent biomarker; using a fluorescence microscope to detect fluorescent cell images that appear clear; and in the acquired images, cell nucleus morphology may be observed while cell morphology may be observed.
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Inventors:
ZHANG HUIMIN (CN)
Application Number:
PCT/CN2021/097587
Publication Date:
December 08, 2022
Filing Date:
June 01, 2021
Export Citation:
Assignee:
ZHANG HUIMIN (CN)
International Classes:
G01N21/64; G01N1/30
Foreign References:
| CN108007754A | 2018-05-08 | |||
| CN103712967A | 2014-04-09 | |||
| CN104350376A | 2015-02-11 | |||
| CN107003242A | 2017-08-01 | |||
| US20110183370A1 | 2011-07-28 |
Other References:
VALDES, P. A. ET AL., NEUROSURG, vol. 115, 2011, pages 11 - 17
XU, H.RICE, B. W., JOURNAL OF BIOMEDICAL OPTICS, vol. 14, 2009, pages 064011
HARADA, K. ET AL., INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 14, 2013, pages 23140 - 23152
KOIZUMI, N. ET AL., ANN. SURG. ONCOL., vol. 20, 2013, pages 3541 - 3548
KONDO, Y. ET AL., INT. J. ONCOL., vol. 45, 2014, pages 41 - 46
COPEMAN SMCOKE FGOULDESBROUGH C., BR MED J., vol. 2, 1929, pages 233 - 42
HAMAMCIOGLU MK ET AL., CLIN NEUROL NEUROSURG, vol. 143, 2016, pages 39 - 45
O'GOSHI KSERUP J., SKI RES TECHNOL., vol. 12, 2006, pages 155 - 61
KOC K. ET AL., BR J NEUROSURG, vol. 22, 2008, pages 99 - 103
HARA T. ET AL., AM J OPHTHALMOL., vol. 126, 1998, pages 560 - 4
KUROIWA T. ET AL., SURG NEUROL, vol. 50, 1998, pages 41 - 8
CHO SS. ET AL., FRONT. SURG., vol. 6, 2019, pages 11
HANSEN DA ET AL., SURG NEUROL.
HAGLUND MM. ET AL., NEUROSURGERY, vol. 38, 1996, pages 308 - 17
MADAJEWSKI B. ET AL.: "CLIN CANCER Res.", AM J NUCL MED MOL IMAGING., vol. 5, 2015, pages 390 - 400
ZEH R. ET AL., PLOS ONE, vol. 12, 2017, pages E0182034
OKUDA T. ET AL., J CLIN NEUROSCI., vol. 19, 2012, pages 1719 - 22
ACERBI F. ET AL., NEUROSURG. FOCUS., vol. 36, 2014, pages E5
OKUDA T. ET AL., J CLIN NEUROSCI., vol. 17, 2010, pages 118 - 121
CHEN B. ET AL., INT J MED SCI., vol. 9, 2012, pages 708 - 714
FRANCAVIGLIA N. ET AL., SURG NEUROL INT., vol. 8, 2017, pages 145
BOWDEN SG. ET AL., NEUROSURGERY, vol. 82, 2018, pages 719 - 27
SUERO MOLINA E. ET AL., J NEUROSURG., vol. 128, 2018, pages 399 - 405
See also references of EP 4160191A4
XU, H.RICE, B. W., JOURNAL OF BIOMEDICAL OPTICS, vol. 14, 2009, pages 064011
HARADA, K. ET AL., INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 14, 2013, pages 23140 - 23152
KOIZUMI, N. ET AL., ANN. SURG. ONCOL., vol. 20, 2013, pages 3541 - 3548
KONDO, Y. ET AL., INT. J. ONCOL., vol. 45, 2014, pages 41 - 46
COPEMAN SMCOKE FGOULDESBROUGH C., BR MED J., vol. 2, 1929, pages 233 - 42
HAMAMCIOGLU MK ET AL., CLIN NEUROL NEUROSURG, vol. 143, 2016, pages 39 - 45
O'GOSHI KSERUP J., SKI RES TECHNOL., vol. 12, 2006, pages 155 - 61
KOC K. ET AL., BR J NEUROSURG, vol. 22, 2008, pages 99 - 103
HARA T. ET AL., AM J OPHTHALMOL., vol. 126, 1998, pages 560 - 4
KUROIWA T. ET AL., SURG NEUROL, vol. 50, 1998, pages 41 - 8
CHO SS. ET AL., FRONT. SURG., vol. 6, 2019, pages 11
HANSEN DA ET AL., SURG NEUROL.
HAGLUND MM. ET AL., NEUROSURGERY, vol. 38, 1996, pages 308 - 17
MADAJEWSKI B. ET AL.: "CLIN CANCER Res.", AM J NUCL MED MOL IMAGING., vol. 5, 2015, pages 390 - 400
ZEH R. ET AL., PLOS ONE, vol. 12, 2017, pages E0182034
OKUDA T. ET AL., J CLIN NEUROSCI., vol. 19, 2012, pages 1719 - 22
ACERBI F. ET AL., NEUROSURG. FOCUS., vol. 36, 2014, pages E5
OKUDA T. ET AL., J CLIN NEUROSCI., vol. 17, 2010, pages 118 - 121
CHEN B. ET AL., INT J MED SCI., vol. 9, 2012, pages 708 - 714
FRANCAVIGLIA N. ET AL., SURG NEUROL INT., vol. 8, 2017, pages 145
BOWDEN SG. ET AL., NEUROSURGERY, vol. 82, 2018, pages 719 - 27
SUERO MOLINA E. ET AL., J NEUROSURG., vol. 128, 2018, pages 399 - 405
See also references of EP 4160191A4
Attorney, Agent or Firm:
NANJING CIPIC INTELLECTUAL PROPERTY FIRM (GENERAL PARTNERSHIP) (CN)
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