Title:
USE OF METHYLENE BLUE AND FLUORESCEIN SODIUM DOUBLE-STAINING IN LIVE CELL IMAGING
Document Type and Number:
WIPO Patent Application WO/2022/252103
Kind Code:
A1
Abstract:
A fluorescent staining method for live cell imaging. The method comprises: performing double-staining on cells by means of using fluorescein sodium and methylene blue, and using a fluorescence microscope to detect and display a clear fluorescent cell image. According to the acquired image, the cell morphology can be observed at the same time that the cell nucleus morphology can be observed.
Inventors:
ZHANG HUIMIN (CN)
Application Number:
PCT/CN2021/097584
Publication Date:
December 08, 2022
Filing Date:
June 01, 2021
Export Citation:
Assignee:
ZHANG HUIMIN (CN)
International Classes:
G01N21/64; G01N1/34
Domestic Patent References:
| WO2000006774A1 | 2000-02-10 |
Foreign References:
| ES2326061A1 | 2009-09-29 | |||
| US20110015529A1 | 2011-01-20 | |||
| CN113348356A | 2021-09-03 | |||
| CN103874913A | 2014-06-18 | |||
| US20160060678A1 | 2016-03-03 |
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HAGLUND MM ET AL., NEUROSURGERY, vol. 38, 1996, pages 308 - 17
MADAJEWSKI B. ET AL., CLIN CANCER RES, vol. 18, 2012, pages 5741 - 51
JIANG JX ET AL., AM J NUCL MED MOL IMAGING, vol. 5, 2015, pages 390 - 400
ZEH R. ET AL., PLOS ONE, vol. 12, 2017, pages e0182034
OKUDA T. ET AL., J CLIN NEUROSCI, vol. 19, 2012, pages 1719 - 22
ACERBI F. ET AL., NEUROSURG.FOCUS., vol. 36, 2014, pages E5
OKUDA T. ET AL., J CLIN NEUROSCI, vol. 17, 2010, pages 118 - 121
CHEN B. ET AL., INT J MED SCI, vol. 9, 2012, pages 708 - 714
FRANCAVIGLIA N. ET AL., SURG NEUROL INT, vol. 8, 2017, pages 145
BOWDEN SG ET AL., NEUROSURGERY, vol. 82, 2018, pages 719 - 27
SUEROMOLINAE ET AL., JNEUROSURG, vol. 128, 2018, pages 399 - 405
XU, H.RICE, B.W., JOURNAL OF BIOMEDICAL OPTICS, vol. 14, 2009, pages 064011
HARADA, K. ET AL., INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 14, 2013, pages 23140 - 23152
KOIZUMI, N. ET AL., ANN.SURG.ONCOL., vol. 20, 2013, pages 3541 - 3548
KONDO, Y. ET AL., INT.J.ONCOL., vol. 45, 2014, pages 41 - 46
COPEMAN SMCOKE FGOULDESBROUGH C, BR MED J, vol. 2, 1929, pages 233 - 42
HAMAMCIOGLU MK ET AL., CLIN NEUROL NEUROSURG, vol. 143, 2016, pages 39 - 45
O'GOSHI KSERUP J, SKI RES TECHNOL, vol. 12, 2006, pages 155 - 61
KOC K. ET AL., BR J NEUROSURG, vol. 22, 2008, pages 99 - 103
HARA T. ET AL., AM J OPHTHALMOL, vol. 126, 1998, pages 560 - 4
KUROIWA T. ET AL., SURG NEUROL, vol. 50, 1998, pages 41 - 8
CHO SS ET AL., FRONT.SURG., vol. 6, 2019, pages 11
HANSEN DA ET AL., SURG NEUROL, vol. 40, 1993, pages 451 - 6
HAGLUND MM. ET AL., NEUROSURGERY, vol. 35, 1994, pages 930 - 40
HAGLUND MM ET AL., NEUROSURGERY, vol. 38, 1996, pages 308 - 17
MADAJEWSKI B. ET AL., CLIN CANCER RES, vol. 18, 2012, pages 5741 - 51
JIANG JX ET AL., AM J NUCL MED MOL IMAGING, vol. 5, 2015, pages 390 - 400
ZEH R. ET AL., PLOS ONE, vol. 12, 2017, pages e0182034
OKUDA T. ET AL., J CLIN NEUROSCI, vol. 19, 2012, pages 1719 - 22
ACERBI F. ET AL., NEUROSURG.FOCUS., vol. 36, 2014, pages E5
OKUDA T. ET AL., J CLIN NEUROSCI, vol. 17, 2010, pages 118 - 121
CHEN B. ET AL., INT J MED SCI, vol. 9, 2012, pages 708 - 714
FRANCAVIGLIA N. ET AL., SURG NEUROL INT, vol. 8, 2017, pages 145
BOWDEN SG ET AL., NEUROSURGERY, vol. 82, 2018, pages 719 - 27
SUEROMOLINAE ET AL., JNEUROSURG, vol. 128, 2018, pages 399 - 405
Attorney, Agent or Firm:
NANJING CIPIC INTELLECTUAL PROPERTY FIRM (GENERAL PARTNERSHIP) (CN)
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