Title:
STEM CELL-DERIVED GASTRULOID MODEL, METHOD FOR CONSTRUCTING SAME, AND USE
Document Type and Number:
WIPO Patent Application WO/2024/060460
Kind Code:
A1
Abstract:
Provided are a stem cell-derived gastruloid model, a method for constructing same, and use. The gastruloid model is acquired by means of in vitro construction from a human pluripotent stem cell. The model can simulate biological events of early embryo development and the key structure of embryos to a certain extent, such as the development of the original endoderm, the establishment of the bilaminar embryonic disc, and the appearance of amnion cavity, amnion cells and primitive streak, and has been confirmed in both the protein level and the transcriptome level. The model can well reproduce key features of the embryo from the peri-implantation period to the gastrula stage, and can be produced by batch induction to serve as an early embryo screening model for medicines, environmental toxicants, and the like, thereby providing a safety test for medicine use and the like for certain early pregnant patients.
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Inventors:
SHA JIAHAO (CN)
YUAN YAN (CN)
YUAN GEGE (CN)
YUAN YAN (CN)
YUAN GEGE (CN)
Application Number:
PCT/CN2022/143274
Publication Date:
March 28, 2024
Filing Date:
December 29, 2022
Export Citation:
Assignee:
INNOVATION CENTER OF SUZHOU NANJING MEDICAL UNIV (CN)
International Classes:
C12N5/0735; C12N5/071; C12N5/074
Foreign References:
| CN113166720A | 2021-07-23 | |||
| CN113337459A | 2021-09-03 |
Other References:
VAN DEN BRINK SUSANNE C.; VAN OUDENAARDEN ALEXANDER: "3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation", TRENDS IN CELL BIOLOGY, ELSEVIER SCIENCE LTD., XX, vol. 31, no. 9, 22 July 2021 (2021-07-22), XX , pages 747 - 759, XP086731991, ISSN: 0962-8924, DOI: 10.1016/j.tcb.2021.06.007
ARIAS ALFONSO MARTINEZ; MARIKAWA YUSUKE; MORIS NAOMI: "Gastruloids: Pluripotent stem cell models of mammalian gastrulation and embryo engineering", DEVELOPMENTAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 488, 7 May 2022 (2022-05-07), AMSTERDAM, NL , pages 35 - 46, XP087099238, ISSN: 0012-1606, DOI: 10.1016/j.ydbio.2022.05.002
ZACHARY T. OLMSTED: "Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, UK, vol. 12, no. 1, UK, XP093155899, ISSN: 2041-1723, DOI: 10.1038/s41467-021-23294-7
WANG XIAOXIAO; XIANG YUNLONG; YU YANG; WANG RAN; ZHANG YU; XU QIANHUA; SUN HAO; ZHAO ZHEN-AO; JIANG XIANGXIANG; WANG XIAOQING; LU : "Formative pluripotent stem cells show features of epiblast cells poised for gastrulation", CELL RESEARCH, SPRINGER SINGAPORE, SINGAPORE, vol. 31, no. 5, 19 February 2021 (2021-02-19), Singapore , pages 526 - 541, XP037899887, ISSN: 1001-0602, DOI: 10.1038/s41422-021-00477-x
ARIAS ALFONSO MARTINEZ; MARIKAWA YUSUKE; MORIS NAOMI: "Gastruloids: Pluripotent stem cell models of mammalian gastrulation and embryo engineering", DEVELOPMENTAL BIOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 488, 7 May 2022 (2022-05-07), AMSTERDAM, NL , pages 35 - 46, XP087099238, ISSN: 0012-1606, DOI: 10.1016/j.ydbio.2022.05.002
ZACHARY T. OLMSTED: "Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, UK, vol. 12, no. 1, UK, XP093155899, ISSN: 2041-1723, DOI: 10.1038/s41467-021-23294-7
WANG XIAOXIAO; XIANG YUNLONG; YU YANG; WANG RAN; ZHANG YU; XU QIANHUA; SUN HAO; ZHAO ZHEN-AO; JIANG XIANGXIANG; WANG XIAOQING; LU : "Formative pluripotent stem cells show features of epiblast cells poised for gastrulation", CELL RESEARCH, SPRINGER SINGAPORE, SINGAPORE, vol. 31, no. 5, 19 February 2021 (2021-02-19), Singapore , pages 526 - 541, XP037899887, ISSN: 1001-0602, DOI: 10.1038/s41422-021-00477-x
Attorney, Agent or Firm:
SINO-CREATIVITY INTELLECTUAL PROPERTY LAW FIRM (CN)
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