Title:
STEM CELLS OBTAINED FROM PULP OF DECIDUOUS OR PERMANENT TEETH AND OF DENTAL GERM, ABLE TO PRODUCE HUMAN BONE TISSUE
Document Type and Number:
WIPO Patent Application WO2006010600
Kind Code:
A3
Abstract:
In this invention is described a method that foresees the isolation of a new subpopulation of stem cells derived from dental pulp, whose differentiation in osteoblasts lead to the subsequent production and employment of a bone tissue, called LAB (Living Autologous Bone). Specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and of dental germs, obtained from human subjects; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular sub-population, which, after differentiation in osteoblasts, is able to produce in vitro an extracellular matrix, identical to that detectable in bone tissue; 3) the use of this selected and differentiated cell population in order to produce autologous bone tissue in vitro, containing vital osteoblasts; 4) the preservation of the LAB under conditions which guarantee cellular vitality; 5) the use of the LAB in donor patients to reconstruct bone tissue, as required in the daily practice in dentistry, maxillo-facial surgery and orthopedics.
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Inventors:
CARINCI FRANCESCO (IT)
D AQUINO RICCARDO (IT)
DE ROSA ALFREDO (IT)
GRAZIANO ANTONIO (IT)
LAINO GREGORIO (IT)
PAPACCIO GIANPAOLO (IT)
D AQUINO RICCARDO (IT)
DE ROSA ALFREDO (IT)
GRAZIANO ANTONIO (IT)
LAINO GREGORIO (IT)
PAPACCIO GIANPAOLO (IT)
Application Number:
PCT/EP2005/008136
Publication Date:
June 22, 2006
Filing Date:
July 27, 2005
Export Citation:
Assignee:
CARINCI FRANCESCO (IT)
D AQUINO RICCARDO (IT)
DE ROSA ALFREDO (IT)
GRAZIANO ANTONIO (IT)
LAINO GREGORIO (IT)
PAPACCIO GIANPAOLO (IT)
D AQUINO RICCARDO (IT)
DE ROSA ALFREDO (IT)
GRAZIANO ANTONIO (IT)
LAINO GREGORIO (IT)
PAPACCIO GIANPAOLO (IT)
International Classes:
C12N5/077; A61K35/12
Foreign References:
| US5306305A | 1994-04-26 | |||
| US20040058442A1 | 2004-03-25 |
Other References:
TSUKAMOTO Y ET AL: "Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts.", ARCHIVES OF ORAL BIOLOGY. DEC 1992, vol. 37, no. 12, December 1992 (1992-12-01), pages 1045 - 1055, XP002373965, ISSN: 0003-9969
MIURA MASAKO ET AL: "SHED: stem cells from human exfoliated deciduous teeth", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 100, no. 10, 13 May 2003 (2003-05-13), pages 5807 - 5812, XP002279268, ISSN: 0027-8424
SHI SONGTAO ET AL: "Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp.", JOURNAL OF BONE AND MINERAL RESEARCH : THE OFFICIAL JOURNAL OF THE AMERICAN SOCIETY FOR BONE AND MINERAL RESEARCH. APR 2003, vol. 18, no. 4, April 2003 (2003-04-01), pages 696 - 704, XP008061980, ISSN: 0884-0431
GRONTHOS S ET AL: "Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 5 DEC 2000, vol. 97, no. 25, 5 December 2000 (2000-12-05), pages 13625 - 13630, XP002201506, ISSN: 0027-8424
LAINO GREGORIO ET AL: "A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB).", JOURNAL OF BONE AND MINERAL RESEARCH : THE OFFICIAL JOURNAL OF THE AMERICAN SOCIETY FOR BONE AND MINERAL RESEARCH., vol. 20, no. 8, August 2005 (2005-08-01), online 28.03.2005, pages 1394 - 1402, XP008061961, ISSN: 0884-0431
LAINO GREGORIO ET AL: "An approachable human adult stem cell source for hard-tissue engineering.", JOURNAL OF CELLULAR PHYSIOLOGY. MAR 2006, vol. 206, no. 3, March 2006 (2006-03-01), pages 693 - 701, XP002373968, ISSN: 0021-9541
MIURA MASAKO ET AL: "SHED: stem cells from human exfoliated deciduous teeth", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 100, no. 10, 13 May 2003 (2003-05-13), pages 5807 - 5812, XP002279268, ISSN: 0027-8424
SHI SONGTAO ET AL: "Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp.", JOURNAL OF BONE AND MINERAL RESEARCH : THE OFFICIAL JOURNAL OF THE AMERICAN SOCIETY FOR BONE AND MINERAL RESEARCH. APR 2003, vol. 18, no. 4, April 2003 (2003-04-01), pages 696 - 704, XP008061980, ISSN: 0884-0431
GRONTHOS S ET AL: "Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 5 DEC 2000, vol. 97, no. 25, 5 December 2000 (2000-12-05), pages 13625 - 13630, XP002201506, ISSN: 0027-8424
LAINO GREGORIO ET AL: "A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB).", JOURNAL OF BONE AND MINERAL RESEARCH : THE OFFICIAL JOURNAL OF THE AMERICAN SOCIETY FOR BONE AND MINERAL RESEARCH., vol. 20, no. 8, August 2005 (2005-08-01), online 28.03.2005, pages 1394 - 1402, XP008061961, ISSN: 0884-0431
LAINO GREGORIO ET AL: "An approachable human adult stem cell source for hard-tissue engineering.", JOURNAL OF CELLULAR PHYSIOLOGY. MAR 2006, vol. 206, no. 3, March 2006 (2006-03-01), pages 693 - 701, XP002373968, ISSN: 0021-9541
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