Title:
CRISPR/CAS SYSTEM-BASED SARS-COV-2 DOUBLE-TARGET RAPID DETECTION METHOD AND KIT
Document Type and Number:
WIPO Patent Application WO/2022/257659
Kind Code:
A1
Abstract:
A CRISPR/Cas system-based SARS-CoV-2 double-target rapid detection method and kit. The method comprises: performing nucleic acid extraction to obtain nucleic acid to be detected; simultaneously amplifying two sites to be detected by using two groups of RT-RAA primers to obtain nucleic acid products, namely a Cas12a targeted N gene amplification product and a Cas13a targeted S gene amplification product; and preparing a CRISPR reaction mixed solution, adding 2 μL of a nucleic acid amplification product into the CRISPR reaction mixed solution, incubating at 37°C for 30 min, and reading fluorescence to obtain a detection result.
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Inventors:
DU YINAN (CN)
XING CHEN (CN)
LIU YAN (CN)
ZHOU JING (CN)
ZHU YATING (CN)
WANG XIAOFENG (CN)
LI QIAN (CN)
ZHANG ZHIKANG (CN)
XING CHEN (CN)
LIU YAN (CN)
ZHOU JING (CN)
ZHU YATING (CN)
WANG XIAOFENG (CN)
LI QIAN (CN)
ZHANG ZHIKANG (CN)
Application Number:
PCT/CN2022/091168
Publication Date:
December 15, 2022
Filing Date:
May 06, 2022
Export Citation:
Assignee:
UNIV ANHUI MEDICAL (CN)
International Classes:
C12Q1/6809; C12Q1/6844; C12Q1/6876
Foreign References:
| CN112877410A | 2021-06-01 | |||
| CN112111602A | 2020-12-22 | |||
| CN110452966A | 2019-11-15 | |||
| CN112795625A | 2021-05-14 |
Other References:
GUO LU, SUN XUEHAN, WANG XINGE, LIANG CHEN, JIANG HAIPING, GAO QINGQIN, DAI MOYU, QU BIN, FANG SEN, MAO YIHUAN, CHEN YANGCAN, FENG: "SARS-CoV-2 detection with CRISPR diagnostics", CELL DISCOVERY, vol. 6, no. 1, 1 December 2020 (2020-12-01), XP055815836, DOI: 10.1038/s41421-020-0174-y
JONATHAN S. GOOTENBERG, OMAR O. ABUDAYYEH, MAX J. KELLNER, JULIA JOUNG, JAMES J. COLLINS, FENG ZHANG: "Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 360, no. 6387, 27 April 2018 (2018-04-27), US , pages 439 - 444, XP055538780, ISSN: 0036-8075, DOI: 10.1126/science.aaq0179
JONATHAN S. GOOTENBERG, OMAR O. ABUDAYYEH, MAX J. KELLNER, JULIA JOUNG, JAMES J. COLLINS, FENG ZHANG: "Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 360, no. 6387, 27 April 2018 (2018-04-27), US , pages 439 - 444, XP055538780, ISSN: 0036-8075, DOI: 10.1126/science.aaq0179
Attorney, Agent or Firm:
BEIJING SHENGXUN IP AGENT LLC (CN)
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