Title:
TAL-EFFECTOR ASSEMBLY PLATFORM, CUSTOMIZED SERVICES, KITS AND ASSAYS
Document Type and Number:
WIPO Patent Application WO/2013/152220
Kind Code:
A3
Abstract:
The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.
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Inventors:
KATZEN FEDERICO (US)
CHESNUT JONATHAN (US)
NOTKA FRANK (DE)
ARNOLD MATTHIAS (DE)
HOFF KEVIN (US)
WHITESIDE STEPHEN (US)
CHESNUT JONATHAN (US)
NOTKA FRANK (DE)
ARNOLD MATTHIAS (DE)
HOFF KEVIN (US)
WHITESIDE STEPHEN (US)
Application Number:
PCT/US2013/035316
Publication Date:
March 20, 2014
Filing Date:
April 04, 2013
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP (US)
GENEART AG (DE)
GENEART AG (DE)
International Classes:
C12N9/22; C12N15/10
Domestic Patent References:
| WO2011072246A2 | 2011-06-16 | |||
| WO2011154393A1 | 2011-12-15 |
Foreign References:
| US20110301073A1 | 2011-12-08 |
Other References:
SANJANA NEVILLE E ET AL: "A transcription activator-like effector toolbox for genome engineering.", January 2012, NATURE PROTOCOLS JAN 2012, VOL. 7, NR. 1, PAGE(S) 171 - 192, ISSN: 1750-2799, XP009170390
ROBERT MORBITZER ET AL: "Assembly of custom TALE-type DNA binding domains by modular cloning", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 39, no. 13, 13 July 2011 (2011-07-13), pages 5790 - 5799, XP002689132, ISSN: 0305-1048, [retrieved on 20110318], DOI: 10.1093/NAR/GKR151
MICHELLE CHRISTIAN ET AL: "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 186, no. 2, 1 October 2010 (2010-10-01), pages 757 - 761, XP002632806, ISSN: 0016-6731, [retrieved on 20100726], DOI: 10.1534/GENETICS.110.120717
WEBER ERNST ET AL: "Assembly of Designer TAL Effectors by Golden Gate Cloning", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 6, no. 5, 19 May 2011 (2011-05-19), XP009162844, ISSN: 1932-6203, DOI: 10.1371/JOURNAL.PONE.0019722
TOMAS CERMAK ET AL: "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 39, no. 12, 1 July 2011 (2011-07-01), pages 1 - 11, XP002659530, ISSN: 1362-4962, [retrieved on 20110414], DOI: 10.1093/NAR/GKR218
ROBERT MORBITZER ET AL: "Assembly of custom TALE-type DNA binding domains by modular cloning", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 39, no. 13, 13 July 2011 (2011-07-13), pages 5790 - 5799, XP002689132, ISSN: 0305-1048, [retrieved on 20110318], DOI: 10.1093/NAR/GKR151
MICHELLE CHRISTIAN ET AL: "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 186, no. 2, 1 October 2010 (2010-10-01), pages 757 - 761, XP002632806, ISSN: 0016-6731, [retrieved on 20100726], DOI: 10.1534/GENETICS.110.120717
WEBER ERNST ET AL: "Assembly of Designer TAL Effectors by Golden Gate Cloning", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 6, no. 5, 19 May 2011 (2011-05-19), XP009162844, ISSN: 1932-6203, DOI: 10.1371/JOURNAL.PONE.0019722
TOMAS CERMAK ET AL: "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 39, no. 12, 1 July 2011 (2011-07-01), pages 1 - 11, XP002659530, ISSN: 1362-4962, [retrieved on 20110414], DOI: 10.1093/NAR/GKR218
Attorney, Agent or Firm:
WHITESIDE, Stephen (5791 Van Allen WayCarlsbad, CA, US)
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