Title:
TEMPERATURE-RESPONSIVE CELL CULTURE BEADS AND METHOD FOR MANUFACTURING SAME
Document Type and Number:
WIPO Patent Application WO/2012/029877
Kind Code:
A1
Abstract:
Temperature-responsive cell culture beads in which a polymer having an electrical charge and exhibiting a varying hydration force within a temperature range of 0−80°C is fixed on the surface of particles comprising a material having a specific gravity of 1.2 or less. Using these beads makes it possible to culture a large number of cells in an efficient manner and to detach the cells efficiently merely by changing the temperature of a substrate surface.
Inventors:
KOBAYASHI JUN (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
Application Number:
PCT/JP2011/069830
Publication Date:
March 08, 2012
Filing Date:
August 31, 2011
Export Citation:
Assignee:
CELLSEED INC (JP)
UNIV TOKYO WOMENS MEDICAL (JP)
KOBAYASHI JUN (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
UNIV TOKYO WOMENS MEDICAL (JP)
KOBAYASHI JUN (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
International Classes:
C12M3/00; C12N5/02
Foreign References:
| JPS6439983A | 1989-02-10 | |||
| JP2005224787A | 2005-08-25 |
Other References:
KIM, M. R. ET AL.: "Swelling Induced Detachment of Chondrocytes Using RGD-Modified Poly(N-isopropylacrylamide) Hydrogel Beads", BIOTECHNOLOGY PROGRESS, vol. 18, 2002, pages 495 - 500
HAN, J. ET AL.: "Immobilization of Coleus blumei plants cells in temperature-sensitive hydrogel", BIOTECHNOLOGY TECHNIQUES, vol. 10, no. 5, 1996, pages 359 - 362
XIA, Y. ET AL.: "Thermal Response of Narrow-Disperse Poly(N-isopropylacrylamide) Prepared by Atom Transfer Radical Polymerization", MACROMOLECULES, vol. 38, 2005, pages 5937 - 5943
KIKUCHI, A. ET AL.: "Temperature-Responsive, Polymer-Modified Surfaces for Green Chromatography", MACROMOLECULAR SYMPOSIA., vol. 207, 2004, pages 217 - 227
HAN, J. ET AL.: "Immobilization of Coleus blumei plants cells in temperature-sensitive hydrogel", BIOTECHNOLOGY TECHNIQUES, vol. 10, no. 5, 1996, pages 359 - 362
XIA, Y. ET AL.: "Thermal Response of Narrow-Disperse Poly(N-isopropylacrylamide) Prepared by Atom Transfer Radical Polymerization", MACROMOLECULES, vol. 38, 2005, pages 5937 - 5943
KIKUCHI, A. ET AL.: "Temperature-Responsive, Polymer-Modified Surfaces for Green Chromatography", MACROMOLECULAR SYMPOSIA., vol. 207, 2004, pages 217 - 227
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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Claims: