Title:
TEMPERATURE-RESPONSIVE SUBSTRATE FOR CELL CULTURE AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2012/029882
Kind Code:
A1
Abstract:
To form a temperature-responsive surface for cell culture by simple processes, said temperature-responsive surface for cell culture being capable of efficiently culturing cells. Cultured cells or a cell sheet can be efficiently removed from the temperature-responsive surface for cell culture by merely changing the temperature of a substrate surface. To cover a substrate surface with a block copolymer, which functions as a temperature-responsive polymer component, in an amount of 0.8-3.0 μg/cm2, said block copolymer having a structure wherein a water-insoluble polymer segment and a temperature-responsive polymer segment are bonded with each other.
Inventors:
NAKAYAMA MASAMICHI (JP)
OKANO TERUO (JP)
OKANO TERUO (JP)
Application Number:
PCT/JP2011/069839
Publication Date:
March 08, 2012
Filing Date:
August 31, 2011
Export Citation:
Assignee:
UNIV TOKYO WOMENS MEDICAL (JP)
NAKAYAMA MASAMICHI (JP)
OKANO TERUO (JP)
NAKAYAMA MASAMICHI (JP)
OKANO TERUO (JP)
International Classes:
C12M3/04; C08F293/00
Domestic Patent References:
| WO2010010837A1 | 2010-01-28 | |||
| WO2010027081A1 | 2010-03-11 |
Foreign References:
| JPH09169850A | 1997-06-30 | |||
| JPH02211865A | 1990-08-23 | |||
| JPH05192138A | 1993-08-03 | |||
| JP2007105311A | 2007-04-26 | |||
| JPH06104061A | 1994-04-15 |
Other References:
See also references of EP 2612902A4
SOFT MATTER, vol. 5, 2009, pages 2937 - 2946
INTERFACE, vol. 4, 2007, pages 1151 - 1157
SOFT MATTER, vol. 5, 2009, pages 2937 - 2946
INTERFACE, vol. 4, 2007, pages 1151 - 1157
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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Claims: