LUO XUEHONG (CA)
TOMASZEWSKI MIROSLAW (CA)
WALPOLE CHRISTOPHER (CA)
CHENG YUN-XING (CA)
LUO XUEHONG (CA)
TOMASZEWSKI MIROSLAW (CA)
WALPOLE CHRISTOPHER (CA)
| US6114390A | 2000-09-05 |
| 1. | A compound of formula (I), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof: wherein R'and R2are independently selected from hydrogen, optionally substituted Ci. 12acyl, optionally substituted C, l2alkyloxycarbonyl, optionally substituted Cl 2alkyl, optionally substituted CI12heteroalkyl, optionally substituted C3 2cycloalkyl, optionally substituted C6 aryl and optionally substituted C2l2heterocyclyl ; W is a linking group that separates the groups linked thereto by one or two atoms; G is N or CH ; R3 is halogen, hydrogen or Cl 6alkyl ; Q is N or CH ; R4 isH or optionally substituted hydrocarbyl; X is a divalent group including first nitrogen atom and a second nitrogen atom, wherein a first group linked to X is linked to the first nitrogen and a second group linked to X is linked to the second nitrogen atom, and wherein the first and second nitrogen atoms are separated by either one carbon atom, or two carbon atoms wherein said two carbon atoms form a double bond therebetween; and Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said sevenmembered ring and aromatic rings, independently, optionally, contains one or more heteroatoms. |
| 2. | A compound of formula (I), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein Rl and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyloxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted arylCI 6alkyl, and optionally substituted heterocyclylCl 6alkyl ; W is a linking group selected fromC (=O),C (=O) OandS (=O) 2; G is N or CH ; R3 is halogen, or hydrogen Q is N or CH; R4 isH, optionally substituted hydrocarbyl, a single bond, or a divalent group; X is represented by (i), (ii), (iii), (iv), (v), (vi), (vii), (viii), (ix), (x), (xi), (xii), (xiii), (xiv), (xv), (xvi), or (xvii) below: (xvii) wherein R5 is selected fromH or optionally substituted alkyl, or a divalent C06group together with R4 to form a portion of a ring, wherein said divalent C06 group optionally contains one or more heteroatoms; R6 is independently selected fromH or optionally substituted alkyl ; Y is represented by formula (III) below : wherein R7 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted arylCs 6alkyl, optionally substituted heteroarylCI 6alkyl,C (=O) OR, C (=O) NHR9,C (=O) NR9R10, SO2NHR9, SO2NR9R10, R11NH2, R11NHR12, R11NR12R13, R11OH, R11OR12, R11SH, R11SR12, or a divalent C0 6group which together with R8 forms a portion of a ring, R isH, halogen, optionally substituted R,OR,SR,S (=O) R12, SO2R12, C (=O) RI2, or a divalent C0 6group which together with the divalent R7 forms the portion of the ring, wherein R9 and R'° are independently C112hydrocarbyl, R11 is C16alkylene, R12 and R'3 are independently Cl 6alkyl ; and Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneCI 6alkyl, or optionally substituted heteroaryleneC16alkyl. |
| 3. | A compound according to claim 2, wherein R'is selected from C16alkyl, C36cycloalkyl, aryl, arylCl 6alkyl or heterocyclyl, heterocyclylCl 6alkyl, wherein said Cl 8alkyl and C3 8cycloalkyl are optionally, independently, subsituted byR,C (=O) R, oxo (=O), sulfo (=S),OH,OR, phenyl, halogen, heterocyclyl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21 and C(=O)OR20, wherein said aryl is optionally substituted byR20, C (=O) R,OH,OR, phenyl, halogen, heterocyclyl, NR2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21 and C(=O)OR20, wherein said heterocyclyl is a five or sixmembered heterocyclyl, wherein said heterocyclyl is optionally substituted byR20, aryl, heteroaryl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21, C(=O)OR20, or oxo (=O) ; R2 is selected from Cl 6alkyl, C3 6cycloalkyl, wherein said Chalky ! and C3 6cycloalkyl are optionally, independently, subsituted by R20, C(=O)R20, oxo (=O), sulfo (=S), OH, OR20, phenyl, halogen, heterocyclyl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21 and C(=O)OR20 ; wherein R20 and R21 are independently Cl 6alkyl ; R3 is selected from bromo, chloro and fluoro; R4 isH, optionally substituted (ClC6) alkyl, or a divalent group together with R5 of X to form a portion of a first ring; W isC (=O), orS (=0) 2 ; GisNorCH ; Q is CH or N ; X is selected from Formulas (i) and (ii), below: (i) (ii) wherein R5 isH, optionally substituted Cl 6alkyl, a bond or a divalent group wherein said bond or divalent group together with R4 forms the portion of the first ring, wherein said first ring is selected from optionally substituted Formulas (a), (b) and (c), (a) (b) (c) wherein when R4 or R5 is substituted, substituents of R4 or R5 are selected from: OH, NH2,O (CIC3) alkyl, CN, oxo (=O),C (=O) O (CIC4) alkyl and halogen. Y is represented by formula (III) below: R7 is optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted arylene which together with R8 forms a portion of a second ring; R8 is R22, OR22, SR22, S(=O)R22, SO2R22, C(=O)R22, or a optionally substituted divalent C06group which together with the divalent R7 forms the portion of the second ring; wherein when R7 or R8 is substituted, substituents of R7 or R8 are halogen, nitro, cyano, R22, C(=O)R22, C(=O)OR22, OH, OR22, C(=O)NH2, C(=O)NHR22, C (=O) NR22R23, SO2NH2, SO2NHR22 or SO2NR22R23; Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneCI 6alkyl, or optionally substituted heteroaryleneCI 6alkyl ; and wherein R22 and R23 are independently Cl 6alkyl. |
| 4. | A compound according to claim 2, wherein Rl is selected from C16alkyl, C36cycloalkyl, aryl, arylCI 6alkyl or heterocyclyl, heterocyclylCI6alkyl, wherein said Cl 6alkyl and C3 6cycloalkyl and aryl are optionally, independently, subsituted byOH,C (=O) OR24,OR24 orNR24R25, wherein said heterocyclyl is derived from pyrrolidinone, fivemembered lactone, fivemembered thiolactone, pyrrolidine, tetrahyrofuran, thiophan, sulfolane, piperidine, piperazine, morpholine, thiomorpholine, dioxane, tetrahydropyran or tetrahydrothiopyran by removing a hydrogen therefrom, wherein said heterocyclyl is optionally substituted by oxo (=O) ; R2 is selected from Cl 6alkyl, C3 6cycloalkyl, wherein said Cl 6alkyl and C3 6cycloalkyl are optionally, independently, subsituted byOH,C (=O) OR24,OR24 and NR24R25; wherein R24 and R25 are independently Cl 6alkyl ; R3 is chloro; R4 isH ; GisNorCH ; QisNorCH ; W isC (=O) ; X is selected from Formulas (i) and (ii), below: wherein R5 isH or Cl 6alkyl ; Y is selected from formulas (d), (e), (f), (g), (h), (j) and (k), below: 030 wherein Z is selected fromC,C (=O),O,N (alkyl) ,NH,S,S (=O) and SO2; Ar1 and Ar2 are, independently, optionally substituted aryl, or optionally subsitituted heteroaryl; R30 is a C). 6hydrocarbyl ; and when Ar1 or Ar2 is represented by a threequarter cycle attached to a ring structure, Arl or Ar2 is fused with said ring structure. |
| 5. | A compound according to claim 4, wherein R'is selected from a group derived from dihydrothiophene2one, pyrrolidinone, fivemembered lactone, or fivemembered thiolactone by removing one hydrogen therefrom, wherein said group is optionally substituted by Chalky ! or phenyl, and CH2C (=O) OC2H5 ; R2 isH orCH3; R5 isH; and Y is selected from structures (1), (m), (n), (o), (p), (q), (r), (s), (t), (u), (v), (w), (x), (y), (z), (al), (bl), (cl), (dl), (el), (fl), (gl) and (hl) below, wherein Rl and R2 are independently selected from hydrogen, optionally substituted C112acyl, optionally substituted Cialkyloxycarbonyl, optionally substituted C112alkyl, optionally substituted C112heteroalkyl, optionally substituted C3t2cycloalkyl, optionally substituted C6 l2aryl and optionally substituted C2, 2heterocyclyl ; W is a linking group selected fromC (=O),C (=O) O and S(=O)2; and wherein R31 is a C16alkyl. |
| 6. | A compound of formula (I), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof: G is N or CH ; R3 is halogen, or hydrogen Q is N or CH ; R4 isH, or optionally substituted hydrocarbyl; X is selected from Formulas (i) and (ii), below wherein R5 isH, or optionally substituted C, 6alkyl ; and Y is optionally subsitituted aryl, or optionally substituted heteroaryl. |
| 7. | A compound selected from: N[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl]hydrazino]3pyridinyl]carbonyl]Nmethylglycine ethyl ester; N[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]3pyridinyl] carbonyl]Nmethylglycine ethyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] thioxomethyl] hydrazino]N(tetrahydro2oxo3thienyl)3pyridine carboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] thioxomethyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl)3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] carbonyl] hydrazino]N(tetrahydro2oxo3thienyl)3pyridinecarboxamide ; N[3chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] amino] methyl] benzoyl]Nmethylglycine ethyl ester; 3chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] amino] methyl]N[[(2S)1ethyl2pyrrolidinyl]methyl]benzamide ; 3chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyl]N [3 (2methyl1piperidinyl)propyl]benzamide ; 3chloro4[[[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] carbonyl] amino] methyl]N [2 (lmethyl2pyrrolidinyl)ethyl]benzamide ; 5chloroN(tetrahydro2oxo3thienyl)6[2[[[(1S,2R)1, 2,3, 4tetrahydro2phenyl1 naphthalenyl] amino] thioxomethyl] hydrazino]3pyridinecarboxamide ; 5Chloro6[2[[[(4chlorophenyl)phenylmethyl]amino]thioxomethyl]hydrazino]N (tetrahydro2oxo3thienyl) 3pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl]3 pyridinecarboxamide ; 5chloro6[2[[(1,2diphenylethyl) amino] thioxomethyl] hydrazino]N (tetrahydro2 oxo3thienyl) 3pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl]hydrazino]N((3S)tetrahydro2oxo3thienyl)3 pyridinecarboxamide ; 5chloro6[2[[(10, 1 1dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N(tetrahydro2oxo3furanyl)3 pyridinecarboxamide; N[[5chloro6[2[[(10,11dihydro5hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl]hydrazino]3pyridinyl] sulfonyl]nmethylglycine, ethyl ester; N[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]3pyridinyl] sulfonyl]Nmethylglycine, ethyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N[[(2S)1ethyl2pyrrolidinyl]methyl]3 pyridinecarboxamide ; N[[3(aminomethyl)cyclohexyl]methyl]5chloro6[2[[(10,11dihydro5H dibenzo [a, d] cyclohepten5yl) amino] carbonyl]hydrazino]3pyridinecarboxamide ; N[[3(aminomethyl)phenyl]methyl]5chloro6[2[[(10,11dihydro5H dibenzo [a, d] cyclohepten5yl) amino] carbonyl]hydrazino]3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [2 (lmethyl2pyrrolidinyl) ethyl] 3 pyridinecarboxamide; 5chloroN[2(diethylamino)ethyl]6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten 5yl) amino] carbonyl] hydrazino] 3pyridinecarboxamide ; 5chloroN[4(diethylamino)1methylbutyl]6[2[[(10,11dihydro5H dibenzo [a, d] cyclohepten5yl) amino] carbonyl]hydrazino]3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]N [2 (lpyrrolidinyl) ethyl]3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [3 (2methyl1piperidinyl)propyl] 3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [3 (dimethylamino) propyl]3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N[[(2R)1ethyl2pyrrolidinyl]methyl]3 pyridinecarboxamide; 3[[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]3pyridinyl] carbonyl]amino]1pyrrolidinecarboxylic acid, 1, 1dimethylethyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N[[(2S)1ethyl2pyrrolidinyl]methyl]3 pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [3 (4morpholinyl) propyl]3pyridinecarboxamide ; N[[3(aminomethyl)cyclohexyl]methyl]5chloro6[2[[(10,11dihydro5H dibenzo [a, d] cyclohepten5yl) amino] thioxomethyl]hydrazino] 3pyridine carboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [3 (4methyl1piperazinyl)propyl] 3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [2 (lmethyl2pyrrolidinyl)ethyl]3 pyridinecarboxamide; 5chloroN[2(diethylamino)ethyl]6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten 5yl)amino] thioxomethyl] hydrazino] 3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [2 (1pyrrolidinyl) ethyl] 3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [3 (2methyl1piperidinyl)propyl] 3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ ( (2R)1ethyl2pyrrolidinyl)methyl]3 pyridinecarboxamide; N[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] thioxomethyl]hydrazino]3pyridinyl] carbonyl]Nmethylglycine, methyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo[a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl)3 pyridinecarboxamide; N[[5chloro6[2[[(10,11dihydro5Hbidenzo [a, d] cyclohepten5 yl) amino] thioxomethyl]hydrazino]3pyridinyl] carbonyl] glycine, ethyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N[2[[2(dimethylamino)ethyl]methylamino]ethyl] 3pyridinecarboxamide ; 2[2chloro4[[(2hydroxyethyl)(phenylmethyl) amino] carbonyl]phenyl]N(10,11 dihydro5Hdibenzo [a, d] cyclohepten5yl) hydrazinecarboxamide ; 3[[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]3pyridinyl]carbonyl]amino]benzoic acid, ethyl ester; 5chloroN(4,4diethoxybutyl)6[2[[(10,11dihydro5Hdibenzo[a, d] cyclohepten5 yl) amino]carbonyl]hydrazino]3pyridinecarboxamide ; 3pyridinecarboxamide, 5chloro6[2[[(10,11dihydro5Hdibenzo[a,d]cyclohepten5 yl) amino]thioxomethyl] hydrazino]NmethylN[2(2pyridinyl) ethyl]; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [2 (lpiperidinyl) ethyl] 3pyridinecarboxamide ; 3pyridinecarboxamide, 5chloro6[2[[(10,11dihydro5Hdibenzo[a, d] cyclohepten5 yl) amino]carbonyl] hydrazino]NmethylN [2 (2pyridinyl) ethyl] ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]N [2 (dimethylamino) ethyl]N (phenylmethyl) 3 pyridinecarboxamide; N[3chloro4[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino] benzoyl]Nmethylglycine, ethyl ester; 2[3chloro5[[4(3chlorophenyl)1piperazinyl]carbonyl]2pyridinyl]N(10,11 dihydro5Hdibenzo [a, d] cyclohepten5yl)hydrazinecarboxamide ; (α1S)α[[[2[3chloro5[[(2hydroxyethyl) (phenylmethyl) amino] carbonyl]2pyridinyl] hydrazino] thioxomethyl] amino]benzeneacetic acid, 1,1dimethylethyl ester; 5chloro6 [2 [ [ (10, 11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [2 (1piperidinyl) ethyl] 3pyridinecarboxamide ; Nbutyl5chloroN(cyanomethyl)6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten 5yl)amino] carbonyl] hydrazino] 3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N[2(dimethylamino) ethyl]N(phenylmethyl)3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N (3fluorophenyl) 3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [3 (dimethylamino)2, 2dimethylpropyl] 3 pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] hydrazino]N [2 (dimethylamino)2 (4methoxyphenyl) ethyl] 3 pyridinecarboxamide; 3[[[5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl]hydrazino]3pyridinyl] carbonyl]amino]benzoic acid, ethyl ester; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino]carbonyl]hydrazino]N[[4[2(dimethylamino)ethoxy]phenyl]methyl 3 pyridinecarboxamide ; 5chloro6 [2 [ [(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N (3iodophenyl)3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3furanyl] 3 pyridinecarboxamide; N [ [5Chloro6 [ [ [ [ (10, 11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyl]3pyridinyl]carbonyl]Nmethylglycine ethyl ester; 5Chloro6[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyl]N(tetrahydro2oxo3thienyl) 3pyridinecarboxamide ; 3Chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyl]N [3 (4methyl1piperazinyl)propyl]benzamide ; 3Chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyllN(tetrahydro1, 1dioxido3thienyl)benzamide ; 3Chloro4[[[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5yl) amino] carbonyl] amino] methyl]N (3methyl5isothiazolyl)benzamide ; Schloro6[2[[(6, 11dihydroSHbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] carbonyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(6, 11dihydro5, 5dioxidodibenzo [b, e] thiepin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydrodibenzo[b,e]thiepin11yl) amino] thioxomethyl] hydrazino] N (tetrahydro2oxo3thienyl) 3pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2c] pyridinS yl) amino] carbonyl] hydrazino]N (tetrahydro2oxo3thienyl) 3pyridinecarboxamide ; 5chloor6[[[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] amino] methyl]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide ; 5chloro6[[[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] amino] methyl]N [ (3R)1methyl2oxo3pyrrolidinyl]3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] carbonyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(6, 11dihydro7methyl5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl]hydrazino]N[(3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[[[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] carbonyl] amino] methyl]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(3chloro10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ (3R)lmethyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(2chloro10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide ; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl]1methylhydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)lmethyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N [ (3R)lmethyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] carbonyl] hydrazino]N [ (3R)lmethyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(10,11dihydro5Hbenzo [4,5] cyclohepta[1, 2c] pyridin5 yl) amino] carbonyl] hydrazino]N (1methyl2oxo3pyrrolidinyl) 3 pyridinecarboxamide; 2[3chloro5[[[(3R)1methyl2oxo3pyrrolidinyl]amino]sulfonyl]2pyridinyl]N(7 fluoro6, 11dihydro5Hbenzo [5,6] cyclohepta[1, 2c] pyridin11yl) hydrazinecarbothioamide; 2[3chloro5[[[(3R)1methyl2oxo3pyrrolidinyl] amino] sulfonyl]2pyridinyl]N (10, 11dihydro5Hdibenzo [a, d] cyclohepten5yl) hydrazinecarbothioamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl]3 pyridinecarboxamide; 5chloro6 [2 [ [ (10, 11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] thioxomethyl] hydrazino]N [ (3R)1methyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)lmethyl5oxo3pyffolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N[(3S)1methyl5oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl)amino]carbonyl]hydrazino]N[(3R)tetrahydro2oxo3furanyl] 3 pyridinecarboxamide; 5chloro6 [2 [ [ (7fluoro10, 1 1dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N [ (3R)tetrahydro2oxo3furanyl] 3 pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d]cyclohepten5 yl) amino] thioxomethyl] hydrazino]N(2oxo1phenyl3pyrrolidinyl) 3 pyridinecarboxamide ; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (3R)2oxo1phenyl3pyrrolidinyl]3 pyridinecarboxamide ; 5chloro6[2[[(10,11dihydro5Hdibenzo [a, d] cyclohepten5 yl) amino] carbonyl] hydrazino]N(2oxo1phenyl3pyrrolidinyl) 3 pyridinecarboxamide; N[[5chloro6[[[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] amino] methyl]3pyridinyl] carbonyl]Nmethylglycine, ethyl ester; N[[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl] carbonyl]glycine, ethyl ester; 5chloro6[2[[(10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N [ (3R)lmethyl2oxo3pyrrolidinyl] 3 pyridinecarboxamide; chloro6[2[[(7fluoro10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N [ (3R)2oxo1phenyl3pyrrolidinyl] 3 pyridinecarboxamide ; 5chloro6[2[[(6, 11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] carbonyl] hydrazino]N [ (3R)tetrahydro2oxo3thienyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl]hydrazino]N[(3R)1methyl5oxo3pyrrolidinyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N[[(2R)tetrahydro2furanyl] methyl]3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N[[(2S)tetrahydro2furanyl] methyl]3 pyridinecarboxamide; 5chloroN[2(diethylamino)ethyl]6[2[[(7fluoro6,11dihydro5H benzo [5, 6] cyclohepta [1, 2c] pyridin11yl) amino] thioxomethyl]hydrazino] 3 pyridinecarboxamide ; 5chloroN[2(dimethylamino)ethyl]6[2[[(7fluoro6,11dihydro5H benzo [5, 6] cyclohepta [1, 2c] pyridin11yl) amino] thioxomethyl] hydrazino]3 pyridinecarboxamide ; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (lR*, 2R*)2hydroxycyclopentyl] 3 pyridinecarboxamide; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyndin11 yl) amino] thioxomethyl] hydrazino]N (2methoxyphenyl) 3pyridinecarboxamide ; 5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (2methoxy3pyridinyl) 3pyridinecarboxamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]Nmethyl acetamide ; [5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl]hydrazino]3pyridinyl]methyl carbamic acid, methyl ester; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]N, 3dimethyl butanamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]Nmethylcyclopropanecarboxamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]Nmethyl3pyridinecarboxamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]Nmethyl4pyridinecarboxamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]3fluoroNmethylbenzamide ; N[5chloro6[2[[(7fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl]4fluoroNmethylbenzamide ; 5chloro6[2[[(10,11dihydro5Hbenzo [4,5] cyclohepta [1, 2b] pyridin5 yl) amino] thioxomethyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl) 3 pyridinecarboxamide; 2[3chloro5[[(2hydroxyethyl)(phenylmethyl)amino]sulfonyl]2pyridinyl]N(10,11 dihydro5Hdibenzo [a, d] cyclohepten5yl) hydrazinecarbothioamide ; 2[3chloro5[[(2hydroxyethyl)(phenylmethyl) amino] sulfonyl]2pyridinyl]N (10, 11 dihydro5Hdibenzo [a, d] cyclohepten5yl) hydrazinecarboxamide ; 2[3chloro5[[[(1ethyl2pyrrolidinyl)methyl] amino] sulfonyl]2pyridinyl]N [7 ethenyl8, 9dihydro6[(lZ)1propenyl]SHbenzocycloheptenSyl] hydrazinecarbothioamide; 5chloro6 [2 [ [ (9fluoro6, 11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [2 (lmethyl2pyrrolidinyl) ethyl] 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [ 1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl) 3 pyridinecarboxamide; N[[5chloro6[2[[(6,11dihydrodibenzo[b,e]thiepin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl] carbonyl]Nmethylglycine, ethyl ester; N[[5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]3pyridinyl] carbonyl]Nmethylglycine, ethyl ester; SchloroN[2(diethylamino) ethyl]6[2[[(6, 11dihydrodibenzo [b, e] thiepin11 yl) amino] thioxomethyl] hydrazino] 3pyridinecarboxamide ; 5chloroN[(1ethyl2pyrrolidinyl)methyl]6[2[[(9fluoro6,11dihydro5H benzo [5, 6] cyclohepta [1, 2c] pyridin11yl) amino] thioxomethyl] hydrazino]3 pyridinecarboxamide ; 5chloroN[2(diethylamino)ethyl]6[2[[(9fluoro6,11dihydro5H benzo [5, 6] cyclohepta [1, 2c] pyridin11yl) amino] thioxomethyl]hydrazino] 3 pyridinecarboxamide; 5chloroN[2(diethylamino)ethyl]6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2 c] pyridin11yl) amino] thioxomethyl] hydrazino] 3pyridinecarboxamide ; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [2 (lpyrrolidinyl) ethyl] 3pyridinecarboxamide ; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (tetrahydro2oxo3furanyl) 3 pyridinecarboxamide ; Schloro6[2[[(6, 11dihydroSHbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [ (1ethyl2pyrrolidinyl) methyl] 3 pyridinecarboxamide ; Schloro6[2[[(6, 11dihydroSHbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (tetrahydro2oxo3furanyl) 3 pyridinecarboxamide ; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl) 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5, 6] cyclohepta[1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [3 (4methyl1piperazinyl) propyl] 3 pyridinecarboxamide; Schloro6[2[[(6, 11dihydroSHbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [3 (4methyl1piperazinyl) propyl] 3 pyridinecarboxamide; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino]thioxomethyl]hydrazino]NmethylN[2(2pyridinyl)ethyl] 3 pyridinecarboxamide; 5chloroN[[(2S)1ethyl2pyrrolidinyl]methyl]6[2[[[4(4 propylcyclohexyl) phenyl] amino] thioxomethyl] hydrazino] 3pyridinecarboxamide ; 5chloroN[[(2S)1ethyl2pyrrolidinyl]methyl]6[2[[[4(4 propylcyclohexyl) phenyl] amino] thioxomethyl] hydrazino]3pyridinecarboxamide ; 5chloro6[2[[(9chloro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N (2hydroxyethyl)N (phenylmethyl) 3 pyridinecarboxamide ; 5chloro6[2[[(9fluoro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [2 (1pyrrolidinyl) ethyl] 3pyridinecarboxamide ; Schloro6[2[[(6, 11dihydroSHbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl]hydrazino]NmethylN[2(2pyridinyl)ethyl] 3 pyridinecarboxamide ; Schloro6[2[[(6, 11dihydrodibenzo [b, e] thiepin11yl) amino] thioxomethyl] hydrazino] N [3 (4methyl1piperazinyl) propyl] 3pyridinecarboxamide ; 5chloroN[2(1methyl2pyrrolidinyl)ethyl]6[2[[[4(4 propylcyclohexyl) phenyl] amino] thioxomethyl]hydrazino] 3pyridinecarboxamide ; 2[3chloro5[[[(1ethyl2pyrrolidinyl)methyl] amino] sulfonyl]2pyridinyl]N [4 (4 propylcyclohexyl)phenyl] hydrazinecarbothioamide ; Schloro6[2[[(6, 11dihydrodibenzo [b, e] thiepin11yl) amino] thioxomethyl] hydrazino] N [2 (lmethyl2pyrrolidinyl) ethyl] 3pyridinecarboxamide ; 5chloro6[2[[(6,11dihydrodibenzo[b,e]thiepin11yl) amino] thioxomethyl] hydrazino] N [ (lethyl2pyrrolidinyl) methyl] 3pyridinecarboxamide ; 5chloro6[2[[(9chloro6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [3 (4methyllpiperazinyl) propyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydro6methyl5,5dioxidodibenzo[cf][1, 2] thiazepin11 yl) amino] thioxomethyl] hydrazino]N (tetrahydro2oxo3thienyl) 3 pyridinecarboxamide; 5chloroN[(1ethyl2pyrrolidinyl)methyl]6[2[[[4(4 propylcyclohexyl) phenyl] amino] thioxomethyl]hydrazino] 3pyridinecarboxamide ; 5chloro6[2[[(6,11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [2 (1methyl2pyrrolidinyl) ethyl] 3 pyridinecarboxamide; 5chloro6[2[[(6,11dihydrodibenzo[b,e]thiepin11yl) amino] thioxomethyl] hydrazino] N [2 (lpyrrolidinyl) ethyl] 3pyridinecarboxamide ; 2[3chloro5[[[(1ethyl2pyrrolidinyl) methyl] amino] sulfonyl]2pyridinyl]N (10, 11 dihydro5Hdibenzo [a, d] cyclohepten5yl) hydrazinecarboxamide ; 5chloroN[(3R)1methyl2oxo3pyrrolidinyl]6[2[[[(1R,2R)1, 2,3, 4tetrahydro2 phenyl1naphthalenyl] amino] thioxomethyl]hydrazino] 3pyridinecarboxamide ; 5chloro6 [2 [ [ (9chloro6, 11dihydro5Hbenzo [5,6] cyclohepta [1, 2c] pyridin11 yl) amino] thioxomethyl] hydrazino]N [2 (1methyl2pyrrolidinyl) ethyl] 3 pyridinecarboxamide; 2[3chloro5[[(2hydroxyethyl)(phenylmethyl) amino] sulfonyl]2pyridinyl]N (diphenylmethyl) hydrazinecarbothioamide; 5chloro6[2[[(6,11dihydro6methyl5,5dioxidodibenzo[c,f][1, 2] thiazepin11 yl) amino] thioxomethyl] hydrazino]N (lmethyl2oxo3pyrrolidinyl) 3 pyridinecarboxamide ; and pharmaceutically acceptable salts thereof. |
| 8. | A compound according to any one of claims 17 for use as a medicament. |
| 9. | The use of a compound according to any one of claims 17 in the manufacture of a medicament for the therapy of pain. |
| 10. | A pharmaceutical composition comprising a compound according to any one of claims 17 and a pharmaceutically acceptable carrier. |
| 11. | A method for the therapy of pain in a warmblooded animal, comprising the step of administering to said animal in need of such therapy a therapeutically effective amount of a compound according to any one of claims 17. |
| 12. | A compound according to any one of claims 17 for use as an antagonist of bradykinin at the B2 receptor. |
| 13. | A method for preparing compounds of Formula I, comprising the steps of: a) reacting a compound of general formula IV, with an isocyanate (YNCO) or thioisocyanate (YNCS), to give a compound of Formula V; b) reacting the compound of Formula V with an amine HNRIR2 in the presence of an amide coupling reagent and an acid scavenger to yield the compound of formula I, Wherein Rl and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyloxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted arylCl 6alkyl, and optionally substituted heterocyclylCl 6alkyl ; TisSorO ; X is represented by formula (i) or (ii), wherein Rs isH, or optionally substituted Cl 6alkyl ; QisN R4 isH ; G is CH or N; R3 is halogen; Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said sevenmembered ring and aromatic rings, independently, optionally, contains one or more heteroatoms; and W isC (=O). |
| 14. | A method of preparing a compound of formula VI comprising the step of reacting a compound of formula VII with a compound of YNCO or YNCS: to form the compound of formula VI: wherein Rl and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyloxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl ; optionally substituted heterocyclyl ; optionally substituted arylC, 6alkyl, and optionally substituted heterocyclylCl 6alkyl ; T is S or 0 ; X is represented by formula (i) or (ii), (i) (ii) wherein R isH, or optionally substituted Cul 6alkyl ; G is CH or N; R3 is halogen; Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said sevenmembered ring and aromatic rings, independently, optionally, contains one or more heteroatoms; and W isC (=O). |
| 15. | A compound of formula (V): wherein G is N or CH ; TisOorS ; R3 is halogen, hydrogen or Chalky ! ; and Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said sevenmembered ring and aromatic rings, independently, optionally, contains one or more heteroatoms. |
| 16. | @. |
| 17. | A compound of formula (VII) : wherein R'and R2 are independently selected from hydrogen, optionally substituted Cl 2acyl, optionally substituted C1_l2alkyloxycarbonyl, optionally substituted Cl 12alkyl, optionally substituted Cii2heteroalkyl, optionally substituted C3 2cycloalkyl, optionally substituted C6 2aryl and optionally substituted C2 2heterocyclyl ; W isC (=O) orS (=0) 2 ; G is N or CH; and R3 is halogen, hydrogen or Cl 6alkyl. |
| 18. | A compound of YNCO or YNCS, wherein Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said seven membered ring and aromatic rings, independently, optionally, contains one or more heteroatoms. |
| 19. | A compound of formula (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof: wherein Rl and R2 are independently selected from hydrogen, optionally substituted CIl2acyl, optionally substituted Cl 12alkyloxycarbonyl, optionally substituted C112alkyl, optionally substituted Cs l2heteroalkyl, optionally substituted C312cycloalkyl, optionally substituted C6l2aryl and optionally substituted C212heterocyclyl ; W is a linking group that separates the groups linked thereto by one or two atoms; G is N or CH ; R3 is halogen, hydrogen or Cl 6alkyl ; Q is N or CH ; R4 isH or optionally substituted hydrocarbyl; X is a divalent group including first nitrogen atom and a second nitrogen atom, wherein a first group linked to X is linked to the first nitrogen and a second group linked to X is linked to the second nitrogen atom, and wherein the first and second nitrogen atoms are separated by either one carbon atom, or two carbon atoms wherein said two carbon atoms form a double bond therebetween; and Y is a group that includes an optionally substituted sevenmembered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said sevenmembered ring, and wherein each of said sevenmembered ring and aromatic rings, independently, optionally, contains one or more heteroatoms. |
| 20. | A compound of formula (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein Rl and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyloxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted arylCl 6alkyl, and optionally substituted heterocyclylCl 6alkyl ; W is a linking group selected fromC (=O),C (=O) OandS (=O) 2; G is N or CH ; R3 is halogen, or hydrogen Q is N or CH ; R4 isH, optionally substituted hydrocarbyl, a single bond, or a divalent group; X is represented by (i), (ii), (iii), (iv), (v), (vi), (vii), (viii), (ix), (x), (xi), (xii), (xiii), (xiv), (xv), (xvi), or (xvii) below: (xvii) wherein R5 is selected fromH or optionally substituted alkyl, or a divalent Co6group together with R4 to form a portion of a ring, wherein said divalent Co6 group optionally contains one or more heteroatoms; R6 is independently selected fromH or optionally substituted alkyl ; Y is represented by formula (III) below: wherein R7 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted arylCl 6alkyl, optionally substituted heteroarylC16alkyl, C(=O)OR9, C (=O) NHR9, C(=O)NR9R10, SO2NHR9, SO2NR9R10, R11NH2, R11NHR12, R11NR12R13, R11OH, R11OR12, R11SH, R11SR12, or a divalent Group which together with R8 forms a portion of a ring, R8 isH, halogen, optionally substituted R12, OR12, SR12, S(=O)R12, SO2R12, C (=O) Ri2, or a divalent Group which together with the divalent R7 forms the portion of the ring, wherein R9 and Rl° are independently C112hydrocarbyl, R11 is C16alkylene, R12 and Rl3 are independently Cl 6alkyl ; and Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneCl 6alkyl, or optionally substituted heteroaryleneC16alkyl. |
| 21. | A compound according to claim 19, wherein R'is selected from C16alkyl, C36cycloalkyl, aryl, arylCl 6alkyl or heterocyclyl, heterocyclylCl 6alkyl, wherein said Cl 8alkyl and C38cycloalkyl are optionally, independently, subsituted byR20,C (O) R20, oxo (=O), sulfo (=S),OH,oR20, phenyl, halogen, heterocyclyl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21 andC (=O) OR20, wherein said aryl is optionally substituted byR20, C (=O) R20,OH,oR20, phenyl, halogen, heterocyclyl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C(=O)NR20R21 and C(=O)OR20, wherein said heterocyclyl is a five or sixmembered heterocyclyl, wherein said heterocyclyl is optionally substituted byR20, aryl, heteroaryl, NH2, NHR20, NR20R21, C(=O)NH2, C(=O)NHR20, C (=O) NR20R21,C (=O) OR20, or oxo (=O) ; R2 is selected from C16alkyl, C36cycloalkyl, wherein said Cl 6alkyl and C3 6cycloalkyl are optionally, independently, subsituted by R20,C (=O) R20, oxo (=O), sulfo (=S),OH,OR, phenyl, halogen, heterocyclyl,NH2,NHR20,NR2°R21, C(=O) NH2, C (=O) NHR20, C(=O)NR20R21 andC (=O) OR20 ; wherein R20 and R21 are independently Cl 6alkyl ; R3 is selected from bromo, chloro and fluoro; R4 isH, optionally substituted (C1C6) alkyl, or a divalent group together with R5 of X to form a portion of a first ring; W isC (=O), orS (=0) 2 ; GisNorCH ; Q is CH or N; X is selected from Formulas (i) and (ii), below: wherein R5 isH, optionally substituted Cl 6alkyl, a bond or a divalent group wherein said bond or divalent group together with R4 forms the portion of the first ring, wherein said first ring is selected from optionally substituted Formulas (a), (b) and (c), wherein when R4 or R5 is substituted, substituents of R4 or R5 are selected from: OH, NH2,O (CC3) alkyl, CN, oxo (=O), C(=O)O(C1C4)alkyl and halogen. Y is represented by formula (III) below: R7 is optionally substituted aryl, optionally substituted heterocyclyl, or optionally substituted arylene which together with R8 forms a portion of a second ring; R8 is R22, OR22, SR22, S(=O)R22, SO2R22, C(=O)R22, or a optionally substituted divalent Co6group which together with the divalent R7 forms the portion of the second ring; wherein when R7or R8is substituted, substituents of R7 or R8 are halogen, nitro, cyano, R22, C(=O)R22, C(=O)OR22, OH, OR22, C(=O)NH2, C(=O)NHR22, C (=O) NR22R23, SO2NH2, SO2NHR22 or SO2NR22R23 ; Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneC16alkyl, or optionally substituted heteroaryleneCI6alkyl ; and wherein R22 and R23 are independently C16alkyl. |
| 22. | A compound according to claim 19, wherein R'is selected from C16alkyl, C36cycloalkyl, aryl, arylC16alkyl or heterocyclyl, heterocyclylC16alkyl, wherein said Cl 6alkyl and C36cycloalkyl and aryl are optionally, independently, subsituted byOH,C (=O) OR24,OR24 or NR24R25, wherein said heterocyclyl is derived from pyrrolidinone, fivemembered lactone, fivemembered thiolactone, pyrrolidine, tetrahyrofuran, thiophan, sulfolane, piperidine, piperazine, morpholine, thiomorpholine, dioxane, tetrahydropyran or tetrahydrothiopyran by removing a hydrogen therefrom, wherein said heterocyclyl is optionally substituted by oxo (=O) ; R2 is selected from C16alkyl, C36cycloalkyl, wherein said Ci. 6alkyl and C36cycloalkyl are optionally, independently, subsituted byOH,C (=O) OR24,OR24 and ; wherein R24 and R25 are independently Cl 6alkyl ; R3 is chloro; R4 isH ; G is N or CH ; QisNorCH ; W isC (=O); X is selected from Formulas (i) and (ii), below: wherein R5 isH or Cl 6alkyl ; Y is selected from formulas (d), (e), (f), (g), (h), (j) and (k), below: wherein Z is selected fromC,C (=O),O,N (alkyl) ,NH,S,S (=O) and SO2 ; Ar'and Ar2 are, independently, optionally substituted aryl, or optionally subsitituted heteroaryl; R30 is a Cl 6hydrocarbyl ; and when Ar'or Ar2 is represented by a threequarter cycle attached to a ring structure, Ar'or Ar2 is fused with said ring structure. |
| 23. | A compound according to claim 21, wherein R1 is C13alkyl ; R2 is C). 6alkyl optionally substituted by halo or heteroaryl, or aryl optionally substituted by halo or heteroaryl; R5 isH; and Y is selected from structures (1), (m), (n), (o), (p), (q), (r), (s), (t), (u), (v), (w), (x), (y), (z), (al), (bl), (cl), (dl), (el), (fl), (gl) and (hl) below, and wherein R31 is a Cl 6alkyl. |
| 24. | A compound according to any one of claims 1822 for use as a medicament. |
| 25. | The use of a compound according to any one of claims 1822 in the manufacture of a medicament for the therapy of pain. |
| 26. | A pharmaceutical composition comprising a compound according to any one of claims 1822 and a pharmaceutically acceptable carrier. |
| 27. | A method for the therapy of pain in a warmblooded animal, comprising the step of administering to said animal in need of such therapy a therapeutically effective amount of a compound according to any one of claims 1822. |
| 28. | A compound according to any one of claims 1822 for use as an antagonist of bradykinin at the B2 receptor. |
| 29. | A method of preparing a compound of formula VI comprising the step of reacting a compound of formula VII with a compound of YNCO or YNCS: to form the compound of formula VI: wherein R'and R2are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyloxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted arylCl 6alkyl, and optionally substituted heterocyclylC, 6alkyl ; T is S or O ; X is represented by formula (i) or (ii), wherein R5 isH, or optionally substituted C). 6alkyl ; G is CH or N; R3 is halogen; Y is represented by formula (III) below: wherein R7is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted arylCl 6alkyl, optionally substituted heteroarylCz 6alkyl,C (=O) OR9, C (=O) NHR9,C (=O) NR9R10, SO2NHR9, SO2NR9R10, R11NH2, R11NHR12, R11NR12R13, R11OH, R11OR12, R11SH, R11SR12, or a divalent C06group which together with R8 forms a portion of a ring, R8isH, halogen, optionally substituted R12, OR12, SR12, S(=O)R12, SO2R12, C (=O) R12, or a divalent C0 6group which together with the divalent R7 forms the portion of the ring, wherein R9 and R10 are independently C112hydrocarbyl, R11 is C16alkylene, R12 and Rl3 are independently Cl 6alkyl ; and Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneC16alkyl, or optionally substituted heteroaryleneCl 6alkyl ; and W isC (=O). |
| 30. | A compound of formula (VII) : wherein R1 and R2 are independently selected from hydrogen, optionally substituted C 2acyl, optionally substituted C112alkyloxycarbonyl, optionally substituted C112alkyl, optionally substituted CiheteroalkyI, optionally substituted C3 2cycloalkyl, optionally substituted C612aryl and optionally substituted C212heterocyclyl ; W isC (=O) or S(=O)2; G is N or CH; and R3 is halogen, hydrogen or C16alkyl. |
| 31. | A compound of YNCO or YNCS, Y is represented by formula (III) below: wherein R7 is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted arylCl 6alkyl, optionally substituted heteroarylC16alkyl, C(=O)OR9, C (=O) NHR9,C (=O) NR9R10, SO2NHR9, SO2NHR9R10, R11NH2, R11NHR12, R11NR12R13, R11OH, R11OR12, R11SH, R11SR12, or a divalent Group which together with R forms a portion of a ring, R8 isH, halogen, optionally substituted 12, OR12, SR12, S(=O)R12, SO2R12, C (=O) R12, or a divalent Group which together with the divalent R7 forms the portion of the ring, wherein R9 and Rl° are independently C112hydrocarbyl, R11 is C16alkylene, R12 and Rl3 are independently Cl 6alkyl ; and Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted aryleneC16alkyl, or optionally substituted heteroaryleneC). 6alkyl. |
The compounds are useful in therapy, and in particular for the treatment of pain, septic shock, pancreatitis, edema, rhinitis, asthma, colitis, arthritis, hepatorenal syndrome, cancer, bacterial and viral infections, ulcerative colitis, and Alzheimer's Disease.
2. Discussion of Relevant Art Two types of bradykinin receptor are known: The B 1 receptor and the B2 receptor. A number of reports indicate an important role for the B2 receptor in the pathophysiology of pain. [e. g. Hall, J. M. , Morton, I. K. M. The pharmacology and immunopharmacology of kinin receptors. In: Farmer SG (Ed). The kinin system. London: Academic Press, 1997; 9-44]. Hence, compounds that are B2 antagonists are useful in the relief of pain, including chronic pain and acute pain, e. g., chronic inflammatory pain, neuropathic pain, back pain, migraine, cancer pain, visceral pain, arthritis pain and post- operative pain.
DETAILED DESCRIPTION OF THE INVENTION Thus, the problem underlying the present invention was to find and obtain new compounds that are useful in treating pain.
Accordingly, in one aspect, the present invention provides compounds that are useful in treating pain.
In another aspect, the present invention provides compounds that are B2 antagonists.
Definitions Unless specified otherwise within this specification, the nomenclature used in this specification generally follows the examples and rules stated in Nomenclature of Organic Chemisty, Sections A, B, C, D, E, F, and H, Pergamon Press, Oxford, 1979, which is incorporated by references herein for its exemplary chemical structure names-and rules on
naming chemical structures. Optionally, a name of a compound may be generated using a chemical naming program: ACD/ChemSketch, Version 5.09/September 2001, Advanced Chemistry Development, Inc., Toronto, Canada.
The term"Cm-n"or"Cm-n group"used alone or as a prefix, refers to any group having m to n carbon atoms, and having 0 to n multivalent heteroatoms selected from 0, S, N and P, wherein m and n are 0 or positive integers, and n>m. For example,"C1_6" would refer to a chemical group having 1 to 6 carbon atoms, and having 0 to 6 multivalent heteroatoms selected from O, S, N and P.
The term"hydrocarbon"used alone or as a suffix or prefix, refers to any structure comprising only carbon and hydrogen atoms up to 14 carbon atoms.
The term"hydrocarbon radical"or"hydrocarbyl"used alone or as a suffix or prefix, refers to any structure as a result of removing one or more hydrogens from a hydrocarbon.
The term"alkyl"used alone or as a suffix or prefix, refers to monovalent straight or branched chain hydrocarbon radicals comprising 1 to about 12 carbon atoms.
The term"alkylene"used alone or as suffix or prefix, refers to divalent straight or branched chain hydrocarbon radicals comprising 1 to about 12 carbon atoms, which serves to links two structures together.
The term"alkenyl"used alone or as suffix or prefix, refers to a monovalent straight or branched chain hydrocarbon radical having at least one carbon-carbon double bond and comprising at least 2 up to about 12 carbon atoms.
The term"alkynyl"used alone or as suffix or prefix, refers to a monovalent straight or branched chain hydrocarbon radical having at least one carbon-carbon triple bond and comprising at least 2 up to about 12 carbon atoms.
The term"cycloalkyl,"used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical comprising at least 3 up to about 12 carbon atoms.
The term"cycloalkenyl"used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical having at least one carbon-carbon double bond and comprising at least 3 up to about 12 carbon atoms.
The term"cycloalkynyl"used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical having at least one carbon-carbon triple bond and comprising about 7 up to about 12 carbon atoms.
The term"aryl"used alone or as suffix or prefix, refers to a monovalent hydrocarbon radical having one or more polyunsaturated carbon rings having aromatic character, (e. g., 4n + 2 delocalized electrons) and comprising 5 up to about 14 carbon atoms.
The term"arylene"used alone or as suffix or prefix, refers to a divalent hydrocarbon radical having one or more polyunsaturated carbon rings having aromatic character, (e. g., 4n + 2 delocalized electrons) and comprising 5 up to about 14 carbon atoms, which serves to links two structures together.
The term"heterocycle"used alone or as a suffix or prefix, refers to a ring- containing structure or molecule having one or more multivalent heteroatoms, independently selected from N, O, P and S, as a part of the ring structure and including at least 3 and up to about 20 atoms in the ring (s). Heterocycle may be saturated or unsaturated, containing one or more double bonds, and heterocycle may contain more than one ring. When a heterocycle contains more than one ring, the rings may be fused or unfused. Fused rings generally refer to at least two rings share two atoms therebetween.
Heterocycle may have aromatic character or may not have aromatic character.
The term"heteroalkyl"used alon or as a suffix or prefix, refers to a radical formed as a result of replacing one or more carbon atom of an alkyl with one or more heteroatoms selected from N, O, P and S.
The term"heteroaromatic"used alone or as a suffix or prefix, refers to a ring- containing structure or molecule having one or more multivalent heteroatoms, independently selected from N, O, P and S, as a part of the ring structure and including at least 3 and up to about 20 atoms in the ring (s), wherein the ring-containing structure or molecule has an aromatic character (e. g., 4n + 2 delocalized electrons).
The term"heterocyclic group,""heterocyclic moiety,""heterocyclic,"or "heterocyclo"used alone or as a suffix or prefix, refers to a radical derived from a heterocycle by removing one or more hydrogens therefrom.
The term"heterocyclyl"used alone or as a suffix or prefix, refers a monovalent radical derived from a heterocycle by removing one hydrogen therefrom.
The term"heterocyclylene"used alone or as a suffix or prefix, refers to a divalent radical derived from a heterocycle by removing two hydrogens therefrom, which serves to links two structures together.
The term"heteroaryl"used alone or as a suffix or prefix, refers to a heterocyclyl having aromatic character.
The term"heterocylcoalkyl"used alone or as a suffix or prefix, refers to a heterocyclyl that does not have aromatic character.
The term"heteroarylene"used alone or as a suffix or prefix, refers to a heterocyclylene having aromatic character.
The term"heterocycloalkylene"used alone or as a suffix or prefix, refers to a heterocyclylene that does not have aromatic character.
The term"six-membered"used as prefix refers to a group having a ring that contains six ring atoms.
The term"five-membered"used as prefix refers to a group having a ring that contains five ring atoms.
A five-membered ring heteroaryl is a heteroaryl with a ring having five ring atoms wherein 1,2 or 3 ring atoms are independently selected from N, O and S.
Exemplary five-membered ring heteroaryls are thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, isoxazolyl, 1,2, 3-triazolyl, tetrazolyl, 1,2, 3- thiadiazolyl, 1,2, 3-oxadiazolyl, 1,2, 4-triazolyl, 1,2, 4-thiadiazolyl, 1,2, 4-oxadiazolyl, 1,3, 4-triazolyl, 1,3, 4-thiadiazolyl, and 1,3, 4- oxadiazolyl.
A six-membered ring heteroaryl is a heteroaryl with a ring having six ring atoms wherein 1,2 or 3 ring atoms are independently selected from N, 0 and S.
Exemplary six-membered ring heteroaryls are pyridyl, pyrazinyl, pyrimidinyl, triazinyl and pyridazinyl.
The term"substituted"used as a prefix refers to a structure, molecule or group, wherein one or more hydrogens are replaced with one or more Ct. nhydrocarbon groups, or one or more chemical groups containing one or more heteroatoms selected from N, O, S, F, Cl, Br, I, and P. Exemplary chemical groups containing one or more heteroatoms include-NO2,-OR,-Cl,-Br,-I,-F,-CF3,-C (=O) R, -C (=O) OH,-NH2,-SH,-NHR,-NR2, -SR,-SO3H,-SO2R,-S (=O) R, -CN,-OH,-C (=O) OR, -C (=O) NR2,-NRC (=O) R, oxo (=O), imino (=NR), thio (=S), and oximino (=N-OR), wherein each"R"is a Cl s2hydrocarbyl. For example, substituted phenyl may refer to nitrophenyl, methoxyphenyl, chlorophenyl, aminophenyl, etc. , wherein the nitro, methoxy, chloro, and amino groups may replace any suitable hydrogen on the phenyl ring.
The term"substituted"used as a suffix of a first structure, molecule or group, followed by one or more names of chemical groups refers to a second structure, molecule or group, which is a result of replacing one or more hydrogens of the first structure, molecule or group with the one or more named chemical groups. For example, a"phenyl substituted by nitro"refers to nitrophenyl.
The term"optionally substituted"refers to both. groups, structures, or molecules that are substituted and those that are not substituted.
Heterocycle includes, for example, monocyclic heterocycles such as: aziridine, oxirane, thiirane, azetidine, oxetane, thietane, pyrrolidine, pyrroline, imidazolidine, pyrazolidine, pyrazoline, dioxolane, sulfolan 2, 3-dihydrofuran, 2,5-dihydrofuran tetrahydrofuran, thiophane, piperidine, 1,2, 3,6-tetrahydro-pyridine, piperazine, morpholine, thiomorpholine, pyran, thiopyran, 2,3-dihydropyran, tetrahydropyran, 1,4- dihydropyridine, 1,4-dioxane, 1,3-dioxane, dioxane, homopiperidine, 2,3, 4,7-tetrahydro- lu-azepin homopiperazine, 1,3-dioxepane, 4, 7-dihydro-1, 3-dioxepin, and hexamethylene oxide.
In addition, heterocycle includes aromatic heterocycles, for example, pyridine, pyrazine, pyrimidine, pyridazine, thiophene, furan, furazan, pyrrole, imidazole, thiazole, oxazole, pyrazole, isothiazole, isoxazole, 1,2, 3-triazole, tetrazole, 1,2, 3-thiadiazole, 1,2, 3- oxadiazole, 1,2, 4-triazole, 1,2, 4-thiadiazole, 1,2, 4-oxadiazole, 1,3, 4-triazole, 1,3, 4- thiadiazole, and 1,3, 4- oxadiazole.
Additionally, heterocycle encompass polycyclic heterocycles, for example, indole, indoline, isoindoline, quinoline, tetrahydroquinoline, isoquinoline, tetrahydroisoquinoline, 1,4-benzodioxan, coumarin, dihydrocoumarin, benzofuran, 2,3- dihydrobenzofuran, isobenzofuran, chromene, chroman, isochroman, xanthene, phenoxathiin, thianthrene, indolizine, isoindole, indazole, purine, phthalazine, naphthyridine, quinoxaline, quinazoline, cinnoline, pteridine, phenanthridine, perimidine, phenanthroline, phenazine, phenothiazine, phenoxazine, 1,2-benzisoxazole, benzothiophene, benzoxazole, benzthiazole, benzimidazole, benztriazole, thioxanthine, carbazole, carboline, acridine, pyrolizidine, and quinolizidine.
In addition to the polycyclic heterocycles described above, heterocycle includes polycyclic heterocycles wherein the ring fusion between two or more rings includes more than one bond common to both rings and more than two atoms common to both rings.
Examples of such bridged heterocycles include quinuclidine, diazabicyclo [2.2. 1] heptane and 7-oxabicyclo [2.2. 1] heptane.
Heterocyclyl includes, for example, monocycle heterocyclyls, such as: aziridinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, pyrrolinyl, imidazolidinyl, pyrazolidinyl, pyrazolinyl, dioxolanyl, sulfolanyl, 2, 3-dihydrofuranyl, 2,5-dihydrofuranyl, tetrahydrofuranyl, thiophanyl, piperidinyl, 1,2, 3, 6-tetrahydro-pyridinyl, piperazinyl, morpholinyl, thiomorpholinyl, pyranyl, thiopyranyl, 2,3-dihydropyranyl, tetrahydropyranyl, 1,4-dihydropyridinyl, 1,4-dioxanyl, 1,3-dioxanyl, dioxanyl, homopiperidinyl, 2,3, 4, 7-tetrahydro-lH-azepinyl, homopiperazinyl, 1,3-dioxepanyl, 4,7- dihydro-1, 3-dioxepinyl, and hexamethylene oxidyl.
In addition, heterocyclyl includes aromatic heterocyclyls or heteroaryl, for example, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, thienyl, furyl, furazanyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, isoxazolyl, 1,2, 3-triazolyl, tetrazolyl, 1,2, 3-thiadiazolyl, 1,2, 3-oxadiazolyl, 1,2, 4-triazolyl, 1,2, 4-thiadiazolyl, 1,2, 4- oxadiazolyl, 1,3, 4-triazolyl, 1,3, 4-thiadiazolyl, and 1,3, 4 oxadiazolyl.
Additionally, heterocyclyl encompasses polycyclic heterocyclyls (including both aromatic or non-aromatic), for example, indolyl, indolinyl, isoindolinyl, quinolinyl, tetrahydroquinolinyl, isoquinolinyl, tetrahydroisoquinolinyl, 1, 4-benzodioxanyl, coumarinyl, dihydrocoumarinyl, benzofuranyl, 2, 3-dihydrobenzofuranyl, isobenzofuranyl, chromenyl, chromanyl, isochromanyl, xanthenyl, phenoxathiinyl, thianthrenyl, indolizinyl, isoindolyl, indazolyl, purinyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, pteridinyl, phenanthridinyl, perimidinyl, phenanthrolinyl, pherazinyl, phenothiazinyl, phenoxazinyl, 1,2-benzisoxazolyl, benzothiophenyl, benzoxazolyl, benzthiazolyl, benzimidazolyl, benztriazolyl, thioxanthinyl, carbazolyl, carbolinyl, acridinyl, pyrolizidinyl, and quinolizidinyl.
In addition to the polycyclic heterocyclyls described above, heterocyclyl includes polycyclic heterocyclyls wherein the ring fusion between two or more rings includes more than one bond common to both rings and more than two atoms common to both rings. Examples of such bridged heterocycles include quinuclidinyl, diazabicyclo [2.2. 1] heptyl; and 7-oxabicyclo [2. 2.1] heptyl.
The term"alkoxy"used alone or as a suffix or prefix, refers to radicals of the general formula-O-R, wherein R is selected from a hydrocarbon radical. Exemplary
alkoxy includes methoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, isobutoxy, cyclopropylmethoxy, allyloxy, and propargyloxy.
The term"amine"or"amino"used alone or as a suffix or prefix, refers to radicals of the general formula-NRR', wherein R and R'are independently selected from hydrogen or a hydrocarbon radical.
"Acyl"used alone, as a prefix or suffix, means-C (=O)-R, wherein R is an optionally substituted hydrocarbyl, hydrogen, amino or alkoxy. Acyl groups include, for example, acetyl, propionyl, benzoyl, phenyl acetyl, carboethoxy, and dimethylcarbamoyl.
Halogen includes fluorine, chlorine, bromine and iodine.
"Halogenated, "used as a prefix of a group, means one or more hydrogens on the group is replaced with one or more halogens.
"RT"or"rt"means room temperature.
A first ring group being"fused"with a second ring group means the first ring and the second ring share at least at least two atoms therebetween.
Description of Preferred Embodiments In one aspect, the invention provides a compound of formula (I) or (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof : wherein Rl and R2 are independently selected from hydrogen, optionally substituted Ci-i2acyl, optionally substituted Cl 12alkyl-oxycarbonyl, optionally substituted Cl 12alkyl, optionally substituted CtheteroalkyI, optionally substih. ited C3-l2cycloalkyl, optionally
substituted C6-) 2aryl, optionally substituted Czheterocyclyl ; optionally substituted aryl- Cl 6alkyl, and optionally substitutedheterocyclyl-C1 6alkyl ; W is a linking group that separates the groups linked thereto by one or two atoms ; GisNorCH ; R3 is halogen, hydrogen or Cl 6alkyl ; Q is N or CH; R4 is-H or optionally substituted hydrocarbyl ; X is a divalent group including first nitrogen atom and a second nitrogen atom, wherein a first group linked to X is linked to the first nitrogen and a second group linked to X is linked to the second nitrogen atom, and wherein the first and second nitrogen atoms are separated by either one carbon atom, or two carbon atoms wherein said two carbon atoms form a double bond therebetween; and Y is a group that includes an optionally substituted seven-membered ring and two optionally substituted aromatic rings, wherein each of the aromatic rings is independently fused with said seven-membered ring, and wherein each of said seven-membered ring and aromatic rings, independently, optionally, contains one or more heteroatoms.
In another aspect, the compounds of the present invention are those of formula (I) or (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein R and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyl-oxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted aryl-CI 6alkyl, and optionally substituted heterocyclyl-CI 6alkyl ; W is a linking group selected from-C (=O)-,.-C (=O) O-and-S (=O) 2-; G is N or CH ; R3 is halogen, or hydrogen Q is N or CH; R4 is-H, or optionally substituted hydrocarbyl; X is a divalent group including first nitrogen atom and a second nitrogen atom, wherein a first group linked to X is linked to the first nitrogen and a second group linked to X is linked to the second nitrogen atom, and wherein the first and second nitrogen atoms are separated by either one carbon atom, or two carbon atoms wherein said two carbon atoms form a double bond therebetween, more particularly X is selected from Formulas (i) and (ii), below
wherein RS is-H, or optionally substituted Cl. 6alkyl ; and Y is optionally substituted aryl, or optionally substituted heteroaryl; more particularly, Y is aryl optionally substituted by a Cl 12hydrocarbyl or heteroaryl optionally substituted by a Cl, 2hydrocarbyl ; most particularly, Y is Cl. 6alkyl-cyclohexyl-phenyl.
In a further aspect, the compounds of the present invention are those of formula (1) or (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein Rl and R2 are independently selected from hydrogen, optionally substituted acyl, optionally substituted alkyl-oxycarbonyl, optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted cycloalkyl, optionally substituted aryl; optionally substituted heterocyclyl; optionally substituted aryl-Cz 6alkyl, and optionally substituted heterocyclyl-Cl 6alkyl ; W is a linking group selected from-C (=O)-,-C (=O) O- and-S (=0) 2- ; G is N or CH ; R3 is halogen, or hydrogen QisNorCH ; R4 is-H, optionally substituted hydrocarbyl, a single bond, or a divalent group; X is represented by (i), (ii), (iii), (iv), (v), (vi), (vii), (viii), (ix), (x), (xi), (xii), (xiii), (xiv), (xv), (xvi), or (xvii) below:
wherein R5 is selected from-H or optionally substituted alkyl, or a divalent C0-6group together with R4 to form a portion of a ring, wherein said divalent Co-6 group optionally contains one or more heteroatoms ; R6 is independently selected from-H or optionally substituted alkyl ; Y is represented by formula (III) below:
wherein Ra is optionally substituted aryl, optionally substituted heteroaryl, optionally substituted aryl-Cl 6alkyl, optionally substituted heteroaryl-Cl-6alkyl,-C (=O) O-R9, -C(=O)NHR9, -C(=O)NR9R10, -SO2NHR9, -SO2NR9R10, -R11NH2, -R11NHR12, -R11NR12R13, -R11OH, -R11OR12, -R11SH, -R11SR12, or a divalent C0-6group which together with R8 forms a portion of a ring, Re is-H, halogen, optionally substituted R12, -OR12, -SR12, -S (=O) R'2,-S02R -C(=O)R12, or a divalent C0-6group which together with the divalent R7 forms the portion of the ring, wherein R9 and Rlo are independently C1-12hydrocarbyl, R11 is C1-6alkylene, R12 and R13 are independently Cl 6aLkyl ; and Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted arylene-Cl 6alkyl, or optionally substituted heteroarylene-Cl 6alkyl.
Particularly, the compounds of the present invention are those of formula (I) or (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein Ri is selected from Cl 6alkyl, C3 6cycloalkyl, aryl, aryl-C1-6alkyl or heterocyclyl, heterocyclyl-Cl 6alkyl, wherein said Cl 6alkyl and C3-6cycloalkyl are optionally, independently, subsituted by R20,-C (=O) R20, oxo (=O), sulfo (=S),-OH,-OR, phenyl, halogen, heterocyclyl, -NH2, -NHR20, -NR20R21, -C(=O) NH2, -C (=O) NHR20, -C(=O)NR20R21 and -C(=O)OR20, wherein said aryl is optionally substituted by-R20, -C(=O)R20, -OH, -OR20, phenyl, halogen, heterocyclyl, -NH2, -NHR20, -NH20R21, -C (=O) NH2, -C (=O) -NHR20, -C (=O) NR20R21 and-C (=O) OR20, wherein said heterocyclyl is a five or six-membered heterocyclyl, wherein said heterocyclyl is optionally substituted by-R20, aryl, heteraryl, -NH2, -NHR20, -NR20R21, -C(=O) NH2, -C (=O) NHR20, -C(=O)NR20R21, -C(=O)OR20, or oxo (=O) ;
R2 is selected from Cl. 6alkyl, C3-6cycloalkyl, wherein said Ci. 6alkyl and C3 6cycloalkyl are optionally, independently, subsituted by R20,-C (=O) Rt°, oxo (=O), sulfo (=S), -OH, -OR20, phenyl, halogen, heterocyclyl, -NH2, -NHR20, -NR20R21, -C(=O)NH2, -C(=O)NHR20, -C(=O)NH20R21 and-C (=O) OR20 ; wherein R20 and R21 are independently C1-6alkyl; R3 is selected from bromo, chloro and fluoro; R4 is-H, optionally substituted (Cl-C6) alkyl or optionally substituted alkenyl, or a divalent group together with R5 of X to form a portion of a ring; W is-C (=O)-, or-S (=0) 2-; GisNorCH ; Q is CH or N ; X is selected from Formulas (i) and (ii), below:
wherein R5 is -H, optionally substituted Cl 6alkyl, a bond or a divalent group wherein said bond or divalent group together with R forms the portion of the ring, wherein the ring is selected from optionally substituted Formula (a), (b) and (c),
wherein when R4 or R5 is substituted, substituents thereof are preferably selected from : -OH, NH2, -O-C1-3alkyl, -CN, oxo (=O),-C (=O) 0--alkyl and halogen.
Y is represented by formula (III) below:
wherein R7 is optionally substituted aryl, optionally substituted heterocyclyl, or. optionally substituted arylene which together with le forms a portion of a ring; Rs is -R22, -OR22, -SR22, -S(=O)R22, -SO2R22, -C(=O)R22, or an optionally substituted divalent C0-6group which together with the divalent R7 forms the portion of the ring; wherein when R7 or e is substituted, preferable substituents thereof are halogen, nitro, cyano, R22, -C(=O)R22, -C(=O)OR22, -OH, -OR22, -C(=O)NH2, -C(=O)NHR22, - C (=O) NR22R23, -SO2NH2, -SO2NHR22 and -SO2NR22R23 ; Ar is optionally substituted arylene, optionally substituted heteroarylene, optionally substituted arylene-Cl. 6alkyl, or optionally substituted heteroarylene-Cl 6alkyl ; and wherein R22 and R23 are independently Cl 6alkyl.
More particularly, the compounds of the present invention are those of formula (I) or (II), pharmaceutically acceptable salts thereof, diasteriomers thereof, enantiomers thereof, or mixtures thereof, wherein Rl is selected from C1-6alkyl, C3-6cycloalkyl, aryl, aryl-C1-6alkyl or heterocyclyl, heterocyclyl-Cs 6alkyl, wherein said Cl 6alkyl and C3-6cycloalkyl and aryl are optionally, independently, subsituted by-OH,-C (=O) OR24,-OR24 and-NR24R25, wherein said heterocyclyl is derived from pyrrolidinone, five-membered lactone, five-membered thiolactone, pyrrolidine, fetrahyrofuran, thiophan, sulfolan, piperidine, piperazine, morpholine, thiomorpholine, dioxane, tetrahydropyran or tetrahydrothiopyran by removing a hydrogen therefrom, wherein said heterocyclyl is optionally substituted by oxo (=O) ; R2 is selected from Cl 6alkyl, C3 6cycloalkyl, wherein said Alkyl and C3 6cycloalkyl are optionally, independently, subsituted by-OH,-C (=O) ORz4,-OR24 and -NR24R25; wherein R24 and R5 are independently Cl 6alkyl ; When the compound of the present invention is represented by formula (I), most particularly, RI is a group derived from dihydrothiophene-2-one, pyrrolidinone, five- membered lactone, or five-membered thilactone by removing a hydrogen therefrom, wherein said group is optionally substituted by C1-3alkyl or phenyl, or -CH2C(=O)OC2H5 ; R2 is-H or-CH3;
When the compound is represented by formula (II), most particularly, R1 is C1- 3alkyl and R is C1-6alkyl optionally substituted by halo or heteroaryl, or aryl optionally substituted by halo or heteroaryl; R3 is chloro; R4 is-H ; GisN ; Q is N or CH ; W is-C (=O)- ; X is selected from Formulas (i), and (ii), below: wherein Rs is -H or Cl 6alkyl. R5 is most particularly-H.
Y is selected from formulas (d), (e), (f), (g), (h), (j) and (k), below:
wherein Z is selected from-C-,-C (=O)-,-0-,-N (-alkyl) -,-NH-,-S-,-S (=0)- and -SO2-; Arl and Ar2 are, independently, optionally substituted aryl, or optionally substituted heteraryl; R30 is a C, 6hydrocarbyl ; when Ar'or Ar2 is represented by a three- quarter cycle attached to a ring structure, Arl or Ar2 is fused with said ring structure.
Y is even more particularly represented by structure (1), (m), (n), (o), (p), (q), (r), (s), (t), (u), (v), (w), (x), (y), (z), (al), (bl), (cl), (dl), (el), (fl), (gl), or (hl) below; Y is most particularly represented by structure (n), (s), (t), (z), (al), (bl), (c1), (dl), (fl), (gl), or (hl) below.
(gel) (h1) wherein R31 is a C1-6alkyl.
Specific examples of compounds of the present invention that may be used in 5 practicing the present invention are listed in Table 1, below.
Table 1 : Compounds. Xw 1 3 N-[[5-chloro-6-[2-[[(10, 11-538 , C rriical aiiie- : M-ass : r : Cmpd.. ; h, ernical Szuc uxe r", he N a A-d t T 'I 4 1f A p-. qy P. kt^ y, 4'-kr_ 1 "F h, 1/\ N- ( [5-chloro-C- [2- [ [ (10, 11- 538 SNH Cl H3C, 0 dihydro-SH-dibenzo [a, d] cyclo- NH ci H3C'0 S HN N Q hepten-5-yl) amino] thioxomethyl]- hydrazino]-3-pyridinyl]-carbonyl]- N-methyl-glycine ethyl ester o mmt c' : r' - , Chemical xme . l ! ! Iass,,. as'. i$&°t. f e :. yr t c y'v S tx Y . '.. r x.. d-. y. £ s # a = _, z ; Spec E 2 N- [ [5-chloro-6- [2- [ [ (10, 11- 522 0/, CH3 dihydro-5H-dibenzo [a, d] cyclo- f (f-/- hepten-5-yl) amino] carbonyl]- 0 hydrazino]-3-pyridinyl] carbonyl]- N-methyl-glycine ethyl ester 3 5-chloro-6-[2-[t (10, 11-dihydro- 538 0 5H-dibenzo [a, d]-cyclohepten-5- ()-NH a)-s NH NH HN 5 yl) amino] thioxomethyl] hydrazino] H N o-N- (tetrahydro-2-oxo-3-thienyl)-3- pyridine-carboxamide 4 5-cbloro-6- [2- [ [ (l 0, 11-dibydro-572 _ HO Ç> 5H-dibenzo [a d]-cyclohepten-5- N-NH C'N yl) amino] thioxomethyl] hydrazino] -han N N- (2-hydroxyethyl)-N- (phenyl- methyl)-3-pyridinecarboxamide 5 ¢) 5-chloro-6-[2-[[(10, 1 1-dihydro-522 o 5H-dibenzo [a, d]-cyclohepten-5- NH s N--NH C'HN-C I yl) amino] carbonyl] hydrazino]-N- o HN o (tetrahydro-2-oxo-3-thienyl)-3- - J pyridinecarboxamide 6 N- [3-chloro-4- [111 (1011 1-dihydro-520 NH Cl H3C. _Or 5H-dibenzo [a, d] cyclohepten-5- yl) amino] carbonyl] amino] methyl] O benzoyl]-N-methyl-glycine ethyl ester 7 3-chloro-4- [ [ [ [ (10, 11-dihydro-5H- 531 NH I benzo [a, d] cyclo-hepten-5- NH CI < HN-H3 yl) amino] carbonylamino] methyl]- hic <'ò N-[[(2S)-I-ethyl-2-pyrrolidinyl]- methyl]-benzamide mp in d. _ :. Ch"'ica Sructuxe,, Chemrcalam. x, : x va ; 8 ./\ 3-chloro-4- [ [ [ [ (10, lI-dihydro-5H- 559 N NH cl cH3 dibenzo [a, d] cyclo-hepten-5-yl)- amino] carbonyl] aminojmethyl]-N- [3-(2-methyl-1-piperidinyl)- propyl]-benzamide 9 O H3C 3-chloro-4-[[[[(10, 11-dihydro-5H-531 L NH ci N dibenzo [a, d]-cyclohepten-5- Ho -NH \, HN yl) amino] carbonyl] amino] methyl]- 0 N- [2- (l-methyl-2-pyrrolidinyl)- ethyl]-benzamide 10 5-chloro-N- (tetrahydro-2-oxo-3- 552 thienyl)-6- [2- [ [ [ (l S, 2R)-1, 2, 3, 4- 0 N c --5 tetrahydro-2-phenyl-1- + (WNH < HN* (I W HN/HN J naphthalenyl] amino] thioxomethyl] ND/0 hydrazino]-3-pyridinecarboxamide 11 cs 5-Chloro-6-[2-[[[(4-chloro-546 phenyl)-phenyl-methyl]-amino]- 0 c . s thioxomethyl]-hydrazino]-N- v WNH < HNA 6__} S//HXN<\ +6 9 (tetrahydro-2-oXo-3-thienyl)-3- pyridine- pyridine- carboxamide 12 5-chloro-6- [2- [ [ (l 0, 11-dihydro-538 ''o 5H-dibenzo [a, d] cyclohepten-5- NH CI I --NH HN yl) amino] tliioxomethyl] liydrazino] lit HN N-N- [ (3R)-tetraliydro-2-oxo-3- thienyl]-3-pyridinecarboxamide 13/\ S-chloro-6- [Z- [ [ (1, 2-diphenyl- 526 ethyl) amino] thioxomethyl] hydrazi 0 . SNH Cl) ~s no]-N-(tetrahydro-2-oxo-3- --NH HN-- S H/V thienyl)-3-pyridinecarboxamide N W m mg . sf. 1 '7 e51. Y . p rv"h,.- . :-'= ; a'x ., r. : ., ? 0 au d- : eriiical'Structur.... , v . : Chriiical., me f :.. =1'Tass : Gmp Ch e s N 14 5-chloro-6- [2- [ [ (10, ll-dihydro- 538 0 5H-dibenzo [a, d] cyclohepten-5- ce NH Hr4l yl) amino] thioxomethyl] hydrazino] i/v \ l HN 0-N- ( (3S)-tetrahydro-2-oxo-3- thienyl)-3-pyridinecarboxamide 15 5-chloro-6- [2- [ [ (10, 11-dihydro-522 0 5H-dibenzo [a, d] cyclohepten-5- yl) amino] thioxomethyl] hydrazino] - N- (tetrahydro-2-oxo-3-furanyl)-3- pyridinecarboxamide 16 2, ßJk J N-[[5-chloro-6-[2-[[(10, 11-574 zizi ¢> cs> o dihydro-Sh-dibenzo [a, d] cyclo- N hepten-5-yl) amino] thioxomethyl]- - hydrazino]-3-pyridinyl] sulfonyl]- n-methyl-glycine, ethyl ester 17oSJ N- [ [5-chloro-6- [2- [ [ (10, ll-58 ce /o dihydro-5H-dibenzo [a, d] cyclo- N NwN I N hepten-5-yl) amino] carbonyl]- zozo hydrazino]-3-pyridinyl] sulfonyl]- N-methyl-glycine, ethyl ester 18 5-chloro-6- [2- [ [ (10, 11-dihydro- 533 SH-dibenzo [a, d] cyclohepten-5- NH CI NH H IN yt) amino] carbonyl] hydrazino]-N- o HN- < \) [ [ (2S)-l-ethyl-2-pyrroUdinyl]- methyl]-3-pyridinecarboxamide 19 N- [ [3- (aminomethyl) cyclohexyl]- 547 NH cl NH, methyl]-5-chloro-6- [2- [ [ (10, 11- NH CI NHp \t o » HXN dihydro-5H-dibenzo [a, d] cyclo- han hepten-5-yl) amino] carbonyl]- hydrazino]-3-pyridinecarboxamide P'C'l in d :. , : heii 1 r ture_.. Chem C ca S uc ,- q,. r. ie 1 ame". ;. ass 20 N- [ [3- (ammomethyl) phenyl]- 541 NH CI NHz methyl]-5-chloro-6- [2- [ [ (10, 11- l H » HN dihydro-5H-dibenzo [a, d] cyclo- NEO hepten-5-yl) amino] carbonyl]- . hydrazino]-3-pyridinecarboxamide 21 n H3C 5-ehloro-6-[2-[[(10, 1 l-dihydro-533 NH ci N 5H-dibenzo [a, d] cyclohepten-5- HAN > NN-i"yl) amino] carbonyl] hydrazino]-N- o HN-- N O [2-(1-methyl-2-pyrrolidinyl) ethyl]-. 3-pyridinecarboxamide 22 5-chloro-N- [2- (diethylamino)- 521 IANH Cl rCH3 ethyl]-6-[2-[[(10, 1 1-dihydro-5H- -NH HN \-CH3 HN dibenzo [a, d] cyclohepten-5-yl)- N O amino] carbonyl] hydrazino]- 3- pyridinecarboxamide 23/--CH3 5-chloro-N- [4- (diethylamino)-l- 563 NH \--CH methylbutyl]-6- [2- [ [ (10, 1 1- N_CN HN dihydro-5H-dibenzo [a, d] cyclo- 1 hepten-5-yl) amino] carbonyl]- hydrazino]-3-pyridinecarboxamide 24 5-chloro-6-[2-[[(10, 11-dihydro-519 SH-dibenzo [a, d] cyclohepten-5-yl)- amino] carbonyl] hydrazino]-N- [2- -HN \/ o (1-pyrrolidinyl) ethyl]-3-pyridine- carboxamide 25\A\ 5-chloro-6- [2- [ [ (10, l 1-dihydro-'61 ci q 5H-dibenzo [a, d] cyclohepten-5- _/ HN yl) amino] carbonyl] hydrazino]-N- [3- (2-methyl-l-piperidinyl)- propyl]-3-pyridinecarboxamide 4. K z- ;-,, 1- : st--'il'., t' : r. j t, . y , :. .,.. ' w x. :, r-., -.-- ,. c d. rv 1 S. uc,. t,,-errii : ca, Cin <Che. ua tur. e. Y 7. .. .,. G Naine, ; Ma s : ; ; ; . weTT'f z". j 1 h.,. t . l. a t . : e.. i S l. Cnt 26 \ 5-chloro-6- [2- [ [ (10, l 1-dihydro-507 nc NH -c"'5H-dibenzo [a, d] cyclohepten-5- NH CI HN yl) amino] carbonyl] hydrazino]-N- [3- (dimethylamino) propyl]- 3- pyridinecarboxamide 27 5-ehloro-6-[2-[[(10, 11-dihydro-533 SH-dibenzo [a, d] cyclohepten-5- NH CI N\H-HN yl) amino] carbonyl] hydrazino]-N- o HN-/H3C U [ [ (2R)-1-ethyl-2-pyrrolidinyl]- methyl]-3-pyridinecarboxamide 28"3c 3- [ [ [5-chloro-6- [2- [ [ (10J 1-'59 cli3 \ L dihydro-5H-dibenzo [a, d] cyclo- NH CI/ N _O . \g) okHN<NX hepten-5-yl) atnino] carbonyll- zehn hydrazino]-3-pyridinyl] carbonyl]- amino]-1-pyrrolidinecarboxylic acid, 1, 1-dimethylethyl ester 29 5-chloro-6- [2- [ [ (10, 1 1-dibydro-549 SH-dibenzo [a, d] cyclohepten-5- NH CI -N \H HIN--') yl) amino] thioxomethyl] hydrazino) S HN /H3C o-N- [ [ (ZS)-1-ethyl-2-pyrrolidinyl]- methyl]-3-pyridinecarboxamide 30 9 5-chloro-6-[2-[[(10, 1 l-dihydro-565 ANH Cl. g/5H-dibenzo [a, d] cyclohepten-5- yl) amino] thioxomethyl] hydrazino] Nô - N- [3- (4-morpholinyl) propyl]- 3- pyridinecarboxamide dz d r,-heiical. Stiuture. k _, C. n 1 mex , rMassy ; , l. y -, f t t. 1.. x a f t n i v r. r t f fW : . : _i : N , . F . i, t rt, xtt '. ..- b.-'y S t i -t. 31/ N- [ [3- (aminomethyl) cyclohexyl]- 563 /methyl]-5-chloro-6- [2- [ [ (10, 11- - s'HN HN dihydro-5H-dibenzo [a, d] cyclo- 0 hepten-5-yl) amino] thioxomethyl]- hydrazino]-3-pyridine- carboxamide 32, CH3 5-chloro-6-[2-p [(10, 11-dihydro-578 SH-dibenzo [a, d] cyclohepten-5- J N-NH C1 HN-J yl) amino] thioxomethyl] hydrazino] . S HN--o _N_ 3-4-meth 1-1-i erazin 1)- Y PP Y propyl]-3-pyridinecarboxamide 33'5-chloro-6- [2- [ [ (10, l 1-dihydro-"549 \/ SH-dibenzo [a, d] cyclohepten-5- NH CI -NH_ HIN-/- yl) aminolthioxomethyllhydrazino] S HN-- N O-N-[2-(1-methyl-2-pyrrolidinyl)- ethyL]-3-pyridinecaIboxamide 34 5-chloro-N- [2- (diethylamino)- 537 NH NH ci rCH3 ethyl]-6-[2-[[(10, 1 l-dihydro-SH- -HN '-cH'dibenzo [a, dJcyclohepten-5-yl)- N O amino] thioxomethyl] hydrazino]- 3-pyridinecarboxamide 35 n 5-chloro-6-[2-[[(10, 11-dihydro-535 SNH Cl ~N/3 5H-dibenzo [a, d] cyclohepten-5- NH ci-N yl) amino] thioxomethyl] hydrazino] s- N N- [2- (1-pyrrolidinyl) etliyl]- 3- pyridinecarboxamide ., ass a I F f n v "d : : Chei a ° ur C in 1 a e, . m , f ic 1 ru. c. e,,. he ac N me Mass, ; 36 5 hloro-6- [2- [ [ (10, 11-dihydro- 577 NH ci FNH HN/5H-dibenzo [a, d] cyclohepten-5- -NH N/N /S HN--' yl) amino] thioxomethyl] hydrazino] N O - N- [3- (2-methyl-1-piperidinyl)- propyl]-3-pyridinecarboxamide 37y. 5-chloro-6-j. 2- [ [ (10, 11-dihydro- 549 NH y N 5H-dibenzo [a, d] cyclohepten-5- HN t S HN<N H3) yl) amino] thioxomethyl] hydrazino] Ho Hz .. [ ( (2R)-l-ethyl-2-pyrrolidinyl)- methyl]-3-pyridinecarboxamide 38 N- [ [5-chloro-6- [2- [ [ (10, 1 I-524 (SNH Ci H3G\ 0/dihydro-5H-dibenzo [a, d] cyclo- Nii cl H3C\---0 S\HN-N hepten-5-yl) amino] thioxomethyl]- N hydrazino]-3-pyridinyllcarbonyl]- N-methyl-glycine, methyl ester 39 110 5-chloro-6- [2- [ [ (l 0, 11-dihydro-556 5H-dibenzo [a, d] cyclohepten-5- NH CI NH NH cl yl) amino] carbonyl] hydrazino]-N- O HN N (2-hydroxyethyl)-N-(phenyl- methyl)-3-pyridinecarboxamide 40 n N-[[5-ehloro-6-[2-[[(10, 1 1-524 0/-CH3 dihyciro-5H-dibenzo [a, d] cyclo- __i NH HN---hepten-5-yl) amino] thioxomethyl]- S>-N hydrazino]-3-pyridinyl] carbonyl]- glycine, ethyl ester . SihGtm S', .. : G. m d.. C eiiiical. Stru tuire.... r eimical Name a s : : """41" \-CH, 3-cMoro-6- [2- [ [ (10, ll-dihydro- 566 NH cl--N 5H-dibenzo [a, d] cyclohepten-5- >, >NH) =\ HNJ CH3 yl) amino] thioxomethyl] hydrazino] N 0 - N- [2- [ [2- (dimethylamino) ethyl]- methylamino]-ethyl]-3-pyridine- carboxamide 42/2- [2-chloro-4- [ [ (2-hydroxyethyl)-'55 (phenylmethyl) amiilo] carbonyl] ph NH ci enyl]-N- (10, 11-dihydro-SH- O HN U dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarboxamide 43 \ o °"'3- [ [ [5-chloro-6- [2- [ [ (10, 11- 570 NH dihydro-SH-dibenzo [a, d] cyclo- 11"in he \/hepten-5-yl) amino] carbonyl]- .. hydrazmo]-3-pyridinyl]-carbonyl]- amino]-benzoic acid, ethyl ester 44/-CH3 5-chloro-N- (4, 4-diethoxybutyl)-6- 566 0 NH CI [2-j [ (10, 11-dihydro-SH-dibenzo- \<.) HN--' O HN N o [a, d] cyclohepten-5-yl) amino]- carbonyl] hydrazino]- 3-pyridine- carboxamide 45 3-pyridinecarboxamide, S-chloro-557 1 NH ci H3CX 6-[2-[[(10, 11-dihydro-5H-dibenzo- \O 5HNHENGN N [aNd] cyclohepten-5-yl) amino]- thioxomethyl] hydrazino]-N- methyl-N- [2- (2-pyridinyl) ethyl]- o m m , . lleiTiicalt GL111't=F'. C'm d : Chexritc I. St ucttire N., VIass « : : 46 O S-chloro-6-[2-[[(10, 1 l-dihydro-533 NH CI . N ! SH-dibenzo [a, d] cyclohepten-5- \t OkH\N<NJw yl) amino] carbonyl] hydrazino]-N- [2- (1-piperidinyl) ethyl]- 3- pyridinecarboxamide 47 3-pyridinecarboxamide, 5-chloro-541 NH Cl H3C 6- [2- [ [ (l 0, 11-dihydro-5H-dibenzo- -NH N N a, d] cyclohepten-5-yl) amino]- O HN carbonyl] hydrazino]-N-methyl-N- [2- (2-pyridinyl) ethyl]- 48/5-chloro-6- [2- [ [ (10, l 1-dihydro-'583 H C-N 5H-dibenzo [a, d] cyclohepten-5-yl)- amino] carbonyl] hydrazino]-N- [2- 0 HN N 0 (dimethylamino) ethyll-N- (phenyl- methyl)-3-pyridinecarboxamide 49 N- [3-chloro-4- [2- [ [ (10, 11- 521 NH C'l13C 0-0/-CH3 dihydro-5H-dibenzo [a, d] cyclo- 0 N-bll hepten-5-yl) amino] carbonyl]- hydrazino] benzoyl]-N-methyl- glycine, ethyl ester 50 2- [3-chloro-5- [ [4- (3-chloro- 601 phenyl)-l-piperazinyl] carbonyl]-2- ci pyridinyl]-N- (l 0, 11-d --N td . o dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarboxamide m Ch.., a..-trtic'r, .. GheiW eal aiire Cy d. ennuc 1. S £u e >,., N "Mass : : ; P 51 HO (a'S)-a-1112- [3-chloro-5- [ [ (2- 570 c ; hydroxyethyl) (phenylmethyl) amin H3'H3g NH ci C-NH'IN-2-pyridinyl]- x- hydrazino] thioxomethyl] amino]- benzeneacetic acid, 1, 1- dimethylethyl ester 52 5-chloro-6- [2- [ [ (10, 1 1-dihydro-549 mNH cl,/NX 5H-dibenzo [a, d] cyclohepten-5- -NH HN I S HN N yl) amino] thioxomethyl] hydrazino] -N-[2-(1-piperidinyl) ethyl]-3- pyridinecarboxamide 53 H3 N-butyl-5-chloro-N- (cyano- 517 methyl)-6- [2- [ [ (10, 11-dihydro-5H- i dibenzo [a, d] cyclohepten-5-yl)- NH U HN HO amino] carbonyl] hydrazino]-3- pyridinecarboxamide 54/CH3 5-chloro-6- [2- [ [ (10, 1 1-dihydro-599 HIC-N su-H-dibenzo [a, d] cyclohepten-5- NH Cl \--NH C yl) amino] thioxomethyl] hydrazino] _/S HN o-N- [2- (dimethylamino) ethyl]-N- (phenylmethyl)-3-pyridine- carboxamide SS ß S-chloro-6-[2-[[(10, 1 l-dihydro-532 F 5H-dibenzo [a, d] cyclohepten-5- Nu ci -N H HN--d yt) aminolthioxomethyl] hydrazino] S HN- N-)-'O-N- (3-fluorophenyl)- 3-pyridine- carboxamide 1'7 De . u c i a% s S . m d'.. herucal'tru, c y' ;' herii'cal. a ,. r. C S ure a z. N me k ;, lYla_s ; : 56/3 5-chtoro-6- [2- [ [ (10, l 1-dihydro-"535 H3C-N SH-dibenzo [a, d] cyclohepten-5- NH C1. CH3 OHN/HN yl) amino] carbonyl] hydrazino]-N- O-HN . N o [3- (dimethylamino)-2, 2-dimethyl- propyl]-3-pyridinecarboxamide 57. 5-chloro-6- [2- [ [ (l 0, 11-dihydro-'599 NH CI H3C ?- ( iOCH3 SH-dibenzo [a, d] cyclohepten-5-yl)- - HHIN U HN8 ; H amino] carbonyl] hydrazino]-N- [2- (dimethylamino)-2- (4-methoxy- phenyl) ethyl]-3-pyridine- carboxamide 58 0 3- [ [ [5-chloro-6- [2- [ [ (10, 1 1-586 " c dihydro-SH-dibenzo [a, d] cyclo- NU CI SHN N /hepten-5-yl) amino] thioxomethyl]- S-HN N O hydrazino]-3-pyridinyl] carbonyl]- amino]-benzoic acid, ethyl ester 59 n"3 S-chloro-6- [2- [ [ (10, 11-dihydro- 599 HIC-N ~\ 5H-dibenzo [a, d] cyclohepten-5- o yl) amino] carbonyl] hydrazino]-N- NH ci- [ [4- [2- (dimethylamino) ethoxy]- -NH_b HN phenyl] methyl]- 3-pyridine- N O carboxamide 60 n 5-chloro-6-[2-[[(10, 1 1-dihydro-640 I 5H-dibenzo [a, d] cyclohepten-5- Nu ci \. HIN yl) aminolthioxomethyllhydrazinol han o-N (3 odophenyl) 3 pyridme carboxamide vs : : ; r.. # erii"al :-a Cm d'. : Chertucal : S-ructtire.". . :. Gh zc me M ss'v 613-chloro-6- [2- [ [ (l 0, 11-dihydro-'522 'o 5H-dibenzo [a, d] cyclohepten-5- NH 0 N--NHNC'HN yl) amino] thioxomethyl] hydrazino] -N- [(3 R)-tetrahydro-2-oxo-3- furanyl]-3-pyridinecarboxamide 62 N [ [5-Chloro-6- [ [ [ [ (10, 11- 521 '-o d--cH3 dihydro-5H-dibenzo [a, d] cyclo- NH CI H3C o ' hepten-5-yl) amino] carbonyl]- amino] methyl]-3-pyridinyl]- carbonyl]-N-methyl-glycine ethyl ester 63 | 5-Chloro-6-[[[[(10n11-dihydro-5H-521 < o dibenzo [a, cl] cyclohepten-5-yl)- NH ci S ---Nli HN-C 1 amino] carbonyl] amino] methyl]-N- o (tetrahydro-2-oxo-3-thienyl)-3- pyridinecarboxamide 64, CH3 3-Chloro-4-[[[[(10, 11-dihydro-5H-560 n rX \jy dibenzo [a, d] cyclohepten-5-yl)- NH CI j X y-NH HN-J amino] carbonyl] amino] methyl]-N- 0 [3- (4-methyl- 1-piperazinyl)- propyl]-benzamide 65 < 3-Chloro-4-[[[[(10, 11-dihydro-5H-538 0 dibenzo (a, ccyclohepten-5-yt)- so amino] carbonyl] amino] methyl]-N- \/_ _ _ o (tetrahydro 1-dioxido-3-thienyl)- benzamide n mt si Gm d ;-,,. . ucal : Stru'ure i- ; CheW cal : c 5 , Name ; Mas.. 66 3-Cbloro-4- [ [ [ [ (l 0, 11-dih ydro-5H-517 mNH Cl CH3 dibenzo [a, dgcyclohepten-S-yl)- arninolearbonyllamino] methyl]-N- l \l (3-methyl-5-isothiazolyl)- benzamide 67 C \§ 5-chloro-6-[2-[[(6, l 1-dihydro-5H-539 ci. 0 benzo [5, 6] cyclohepta [1, 2- H CI N/S j)-N.. o.,)-s ') r-H \= R- (j c] pyridm-ll- H Yl) amino] thioxomethyl] hydrazino]. - N- [ ( R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide 68 N 5-chloro-6- [2- [ [ (10, 1 1-dihydro-539 o SH-benzo [4, 5] cyclohepta [1, 2- H c b] pyridin-5- H yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide 69 v 5-chloro-6- [2- [j (7-fluoro-6, 11- 557 o dihydro-5H- N Hcl H < kN\HA 6kJ benzo [S, 6] cyclohepta [1, 2- N o c] pyridin-l l- yl) amino] thioxomethyl] hydrazino] - N- [ (3 R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide . g > mt ! Cm : >Cem c St u. ckue : C m Na ",. :, j Mass :' . fT, r I n-ra r k,, s. 7 v 1 0 , a. i t t, wEs, 70 <N\ S-chloro-6-[2-[[(7-fluoro-6, 11-541 o dihydro-5H- N H CI H S r .- benzo [5, 6] cyclohepta [1, 2- F 0-J"-A N o c] pyridm-11- yl) amino] carbonyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-thienyl]- 3-pyridinecarboxamide 71 S-chloro-6-[2-[[(6, 11-dihydro-5, 5-588 ° dioxidodibenzo [b, e] thiepin-11- 0--S N Hci yl) amino] thioxomethyl] hydrazino] HAN [ (3R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide 72 3 S-chloro-6-[2-([(6, 11-556 . dihydrodibenzo [b, e] thiepin-ll-. cl yl) amino] thioxomethyl] hydrazino] - N-tetrah dro-2-oxo-3-thien 1- w o Y y) 3-pyridinecarboxamide 73 n S-chloro-6-[2-[[(10, 11-dihydro-523 o SH-benzo [4, 5] cyclohepta [1, 2- N ci H N\ c] pyridin-5- 0 N H yl) amino] carbonyl] hydrazino]-N- o Y) Y Y (tetrahydro-2-oxo-3-thienyl)-3- pyridinecarboxamide 74 5N S-chloro-6-[[[[(7-fluoro-6, 11-556 H o dihydro-SH- N Hci H s N\, benzo [5, 6] cyclohepta [1, 2- F cpyridm 1 yl) amino] thioxomethyl] amino] met hyl]-N- [ (3R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide X m<X r Cm : hemical,-Sructu're, u. ; Cheriiical Na e,, ;,, ;. a 75 (\i 5-chloro-6-[[[[(7-fluoro-6, 11-553 AN H Cl WNw dihydro-5H- ''N --N N--- b5, 6] cyclohepta [1, 2- V F \ I S IPYridin-11- yl) amino] thioxomethyl] amino] met hyl]-N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 76 N S-chloro-6-[2-[[(7-fluoro-6, 11-554 ° dihydro-5H- N n ci H N -. N N benzo [5, 6] cyclohepta [1, 2- s N H N/0 c] pymdm-11- yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 77 N 5-chloro-6- [2- [ [ (7-fluoro-6, 1 1-538 H dihydro-5H- N ci N -N \ benzo [5, 6] cyclohepta [1, 2- N c] pyridm 11 yl) amino] carbonyl] hydrazino]-N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide m < m X zip ,. v 'a <s t : : m d, CheriiicalStructure e p :. .., , Ch myal lane, u r, lIass : : t : r. a, f i 1 i 4 . f 4, 7 ; ue, ,, S 78/5-chloro-6- [2- [ [ (6, l l-dihydro-7-550 ci O methyl-5H- zon N s cl benzo [5, 6] cyclohepta [1, 2- S H N e N c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 79 (\% 5-chloro-6-[2-[[(6, 11-dihydro-5H-536 AH ci 0 N benzo [5, 6] cyclohepta [1, 2- H N /c] pyridin-11- s yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 80 (NV S-chloro-6-[[[[(7-fluoro-6, 11-537 dihydro-SH- H ho beiizo [5, 6] cyclohepta [1, 2- F l N O c] pyridin-11- yl) amino] carbonyl] amino] methyl- N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 81 X o S-chloro-6-[2-[[(3-chloro-10, 11-569 H H dihydro-5H-.. N H cri H dibenzo [a, d] cyclohepten-5- N O yl) amino] thioxomethyl] hydrazino] ci -N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide . rye' r ; r t,. d. y m thexW c 1 S.. : u e >,, Cemicl Name r lV. as'''- : 2 t 4 t . : 45 i, K7 1", p.. NLr.' ; y 82 "' 5-chloro-6- [2- [ [ (2-chloro-10Jl- 569 dihydro-SH- N, H CI Ni dibenzo [a, d] cyclohepten-5- /S,"i v/ "o yl) amino] thioxomethyl] hydrazino] cri -N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 83 6- [2- [ [ (7-fluoro-6, 11-dihydro-5H- 520 (% benzo [5, 6] cyclohepta [1, 2- N - SNN N c] pyridin-1l- N O yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 84 N 5-chloro-6- [2- [ [ (7-fluoro-6, 1 I-568 (H dihydro-5H- N cri \N benzo [5, 6] cyclohepta [1, 2- F \ I/N I yl) amino] thioxomethyl]-1- methylhydrazino]-N- [ (3R)-l- methyl-2-oxo-3-pyrrolidinyl]-3- pyridinecarboxamide 85 S-chloro-6-[2-[[(9-fluoro-6, 11-554 H ° dihydro-SH- N H CI H N /-N 'IN benzo [5, 6] cyclohepta [1, 2- c] pyridm-11- H yl) amino] thioxomethyl] hydrazino] -N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide MiLeal : ' me .,. r..., C : ii d. .. r : Cheriiica'Sruct ve K-°A y. r=. p,., u. s, * ? ChemLCal Naine y Mass. :. , r X, ; d n fY'V e. 86 Nn. 5-chloro-6-[2-[[(7-fluoro-10, 11-554 H dihydro-5H- N H CI H N/ benzo [4, 5] cyclohepta [1, 2- s N F yl) amino] thioxomethyl] hydrazino] -N-[(3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 87 N 5-chloro-6- [2- [ [ (9-fluoro-10, 1 I-538 . N NV dihydro-5H- N cri zu benzo [4, 5] cyclohepta [1, 2- H N/O N O b] pyridin-5- yl) amino] carbonyl] hydrazino]-N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 88 n 5-chloro-6-[2-t [(10, 11-dihydro-520 o 5H-benzo [4, 5] cyclohepta [1, 2- N CI ' a--N N- (I c] pyridin-5- -, /1 amino carbon 1 h drazino-N- N)'< u (1-methyl-2-oxo-3-pyrrolidinyl)- 3-pyridinecarboxamide 89 N 2- [3-chloro-5- [ [ [ (3R)-l-methyl-2-590 oxo-3- N Hci H N Ft Ht/y s\ pyrrolidinyl] amino] sulfonyl]-2- F-\ M/' pyridinyl]-N- (7-fluoro-6, 11- dihydro-SH- benzo [5, 6] cyclohepta [1, 2- c] pyridin-11-yl)- hydrazinecarbothioamide g m v X m c1"Ghemical S'tucure. : : Chmiiical.., > e =., : r, . P, k= n Na. m w lYlasa _ 1 ,, x a S ec : 90 3 2-[3-chloro-5-[[[(3R)-1-methyl-2-571 oxo-3- N H ci H pyrrolidinyl] amino] sulfonyl]-2- \/H v/ss N o o pyridinyl]-N- (10, 11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarbothioamide 91. 5-chloro-6- [2- [ [ (10, l 1-dihydro-'519 'H o 5H-dibenzo [a, d] cyclohepten-5- N Hci H N, /-N \ N-, b yl) amino] carbonyl] hydrazino]-N- or H NO [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 92 5-ehloro-6-[2-[[(10, 11-dihydro-535 ''H o 5H-dibenzo [a, d] cyclohepten-5- Cl N \ yl) amino] thioxomethyl] hydrazino] H N o-N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 93 C) 5-chloro-6-[2-[[(6, 11-dihydro-5H-536 benzo [5, 6] cyclohepta [1, 2- N > S H 40 c] pyridin-11- S l N/O O yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-5-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide e m dx ;.... Chemuca : S'tructiii-e Cherriical Na,'. e x .. x 1 ! Iass ' ass 94 '"5-chloro-6- [2- [ [ (6, ll-dihydro-5H- 536 H benzo [5, 6] cyclohepta [1, 2- N H cl'H N cl pyridin- I I- \/H /o yl) amino] thioxomethyl] hydrazino] - N- [ (3 S)-1-methyl-5-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 955-cMoro-6- [2- [ [ (9-fluoro-10, H-"525 ci ° dihydro-5H- N ti ci H benzo [4, 5] cyclohepta [1, 2- F H b] pyridm-5- yl) amino] carbonyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-furanyl]- 3-pyridinecarboxamide 96 ND 5-chloro-6-[2-[[(7-fluoro-10, 11-541 ° dihydro-5H- N Nt benzo [4, 5] cyclohepta [1, 2- H N b P pyridin-5- F yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-tetrahydro-2-oxo-3- furanyl]-3-pyridinecarboxamide 97 5-chloro-6- [2- [ [ (10, 1 1-dihydro-597 ci 5H-dibenzo [a, d] cyclohepten-5- -NN'IN X SH H yl) amino] thioxemethyl] hydrazino] I H - N- (2-oxo-1-phenyl-3- pyrrolidinyl)-3- pyridinecarboxamide r X mX Cn d a-. A' Checal. S : ructiire GheriiicaI Naine : .. a- ;. ° : ass-TH ; 1' n t f. j. ., t. t i ? 4 n < t, f vn- , 1^' r7 G.,-i ; 'i. J a 1 7 x. 5 1 x'9 't ;'r 1 1 9. 98 (Nxd S-chloro-6-[2-[[(7-fluoro-6, 11-616 ci dihydro-5H- IN benzo [5, 6] cyclohepta [1, 2- F c] pyridin-l l- yl) amino] thioxomethyl] hydrazino] '-N- [ (3R)-2-oxo-l-phenyl-3- pyrrolidinyl]-3- pyridinecarboxamide 99 5-chloro-6- [2- [ [ (10, l 1-dihydro-581 H 5H-dibenzo [a, d] cyclohepten-5- yl) amino] carbonyl] hydrazino]-N- (2-oxo-l-phenyl-3-pyrrolidinyl)- 3-pyridinecarboxamide 100 X H N-[[S-chloro-6-[[[[(7-fluoro-6, 11-556 H dihydro-5H- N o benzo [5, 6] cyclohepta [1, 2- c] pyridin-11- yl) amino] thioxomethyl] amino] met hyl]-3-pyridinyl] carbonyl]-N- methyl-glycine, ethyl ester 101 N-[[S-chloro-6-[2-[[(7-fluoro-543 ci. 6, 11-dihydro-5H- N cri S _R N_-N benzo [5, 6] cyclohepta [1, 2- N 0 c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinyl] carbonyl]- glycine, ethyl ester o mmX , r. Je Z , C : d, Cheuucal : Str, wcture... Ceinical ame.. ... s,. wr i x ', t s .. l. : i 0. , c y, c it. ; ns , _ k s a d z. i fYG.., S''De'. irr ; . 102 Nn 5-chloro-6-[2-[[(10, 11-dihydro-536 SH-benzo [4, 5] cyclohepta [1, 2- N Hci H N -N 11 b] pyridin-5- v l s H vl . N O yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-1-methyl-2-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide 103 N4) 5-chloro-6-[2- [(7-Ruoro-10 11-616 0 dihydro-SH- benzo [4, 5] cyclohepta [1, 2- N bd pyridin-5- yl) amino] thioxomethyl] hydrazino] - N- [ (3R)-2-oxo-1-phenyl-3- pyrrolidinyl]-3- pyridinecarboxamide 104 5-chloro-6- [2- [ [ (6, 11-dihydro-5H- 523 -' H o benzo [5, 6] cyclohepta [1, 2- N Hci H c] pyridin-11- \ o H / tb H NJ O yl) amino] carbonyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-thienyl]- 3-pyridinecarboxamide 105 (\8 5-chloro-6-[2-[[(7-fluoro-6, 11-554 dihydro-SH- N cul '>-N N benzo [5, 6] cyclohepta [ 1, 2- F \/H o c] pyridin-11-. yl) amino] thioxomethyl] hydrazino] -N- [ (3R)- I-methyl-5-oxo-3- pyrrolidinyl]-3- pyridinecarboxamide r mmg Ch, emcal Sfiructure r , % Chemical.'arne ". i. , s F 7. f't .. i, j 1 1 if . 5 . I 106 N 5-chloro-6- [2- [ [ (7-fluoro-6, 1 I-541 dihydro-SH- N Hci F, 6] cyclohepta H, 2- - /-'"' benzo [5, 6] cyclohepta [1, 2- o ,./c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - N- [ [ (2R)-tetrahydro-2- furanyl] methyl]- 3- pyridinecarboxamide 107 N 5-chloro-6- [2- [ [ (7-fluoro-6, 1 1-541 (N H Cl dihydro-5H- N Hcl IH benzo [5, 6] cyclohepta [1, 2- N N O O c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - N- [ [(2 S)-tetrahydro-2- furanyl] methyl]- 3- pyridinecarboxamide 108) S-chlolo-N-[2-556 (diethylamino) ethyl]-6- [2- [ [ (7- N Hci >-t4\ N-\- fluoro-6, 11-dihydro-SH- s N N O benzo [5, 6] cyclohepta [1, 2- cpyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinecarboxamide 109 g 5-chloro-N-[2-528 H (dimethylamino) ethytl-6- [2- ( [ (7- N ci H IIH fluoro-6, 1 1-dihydro-5H- zon N benzo [5, 6] cyclohepta [1, 2- c) pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinecarboxamide zg d :. =.. C eiuucal :. Structuire -Chemiical aine. as-. - Cm 1,, r. r , 1T" vVI s.,. w : k fi ^y,'4 s F r c. , a, S ec 110 C 5-chloro-6-[2-[[(7-fluoro-6, 11-541 X dihydro-SH- N ci N\ IN benzo [5, 6] cyclohepta [1, 2- F I S H . N O OH c] pyridin-l l- yl) amino] thioxomethyl] hydrazino] - N- [ (1R*, 2R*)-2- hydroxycyclopentyl]-3- pyridinecarboxamide 111 2 9/5-chloro-6-[2-[[(7-fluoro-6, 11-563 ° dihydro-SH- H ho -N \ N benzo [5, 6] cyclohepta [1, 2- S N N O c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - N- (2-methoxyphenyl)- 3- pyridinecarboxamide 112 CN/5-chloro-6-[2-[[(7-fluoro-6, 11-564 N ci °-N dihydro-5H- N >-N\ IN benzo [5, 6] cyclohepta [1, 2- F \ I c] pyridm-11- yl) amino] thioxomethyl] hydrazino] -N- (2-methoxy-3-pyridinyl)- 3- pyridinecarboxamide 113 N N- [5-chloro-6- [2- [ [ (7-fluoro-6, 11- 485 tNH H cl o dihydro-5H- N H ci 0 benzo [5, 6] cyclohepta [1, 2- s N N c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinyl]-N-methyl-acetamide ,-C " r i.-, e t, : 1 4 Y 4 C'in d : . C. henii al. t-"rea. , . Che" ic. l. c S ructur u ; m Name, Tlass : 114 ! [5-chloro-6- [2- [ [ (7-fhioro-6, ll- 501 dihydro-5H- H/ t4NN N 0 benzo [5, 6] cyclohepta [1, 2- l S c] pyridin-1l- yl) aminothioxomethyl] hydrazino - 3-pyridinyl] methyl- carbamic acid, methyl ester 115/ ; N- [5-chloro-6- [2- [ [ (7-fluoro-6, 11- 527 AN H dihydro-SH- N ici benzo [5, 6] cyclohepta [1, 2- s N N N c] pyridin-l l- yl) amino] thioxomethyl] hydrazino] - 3-pyridinyl]-N, 3-dimethyl- butanamide N 116N- [5-chloro-6- [2- [ [ (7-auoro-6, 11- 511 dihydro-5H- N Hcl 0 benzo [5, 6] cyclohepta [1, 2- F S H N c] pyridin-11- yl) amino] thioxomethyl] hydrazino] -3-pyridinyl]-N-methyl- cyclopropanecarboxamide 117/ ; N- [5-chloro-6- [2- [ [ (7-fluoro-6, 11- 548 H dihydro-5H- N ici t \ benzo [5, 6] cyclohepta [1, 2- F \ I S H c] Pyridin-11- yl) amino] thioxomethyl] hydrazino] -3-pyridinyl]-N-methyl-3- pyridinecarboxamide . ;. : y : $' _.. n 9.. 3 . T. _.. 1 r i. i : f Gin d : : :. Ghemic. IS'txuctii' ; e Chemi c1 aine r 'Mas : , e s f a . ' t a 1, , u : <TP f {', . ,"'13 Bq ; o ', 4irz 7y I1, y, a.."u. r. i 4,"r ! , 118 N N- [5-chloro-6- [2- [ [ (7-fluoro-6, 1 1-548 dihydro-5H- N H ci 0 benzo [5, 6] cyclohepta [1, 2- s N c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinyl]-N-methyl-4- pyridinecarboxamide 119iN- [5-chloro-6- [2- [ [ (7-Quoro-6, ll- 565 (WNH \ H dihydro-5H- 4 \ benzo [5, 6] cyclohepta [1, 2- s 'F /S H \/N F c] pyridm-11- yl) amino] thioxomethyl] hydrazino] -3-pyridinyl]-3-fluoro-N-methyl- benzamide 120 N N- [5-chloro-6- [2- [ [ (7-fluoro-6, 11- 565 dihydro-5H- benzo [5, 6] cyclohepta [1, 2- N c] pyridin-11- yl) amino] thioxomethyl] hydrazino] -3-pyridinyl]-4-fluoro-N-methyl- benzamide 121 5-chloro-6- [2- [ [ (10, 11-dihydro- 573 ho SH-benzo [4, 5] cyclohepta [1, 2- H N N ci pyridin-5- N O s N yl) ammo] thioxomethyl] hydrazmo] - N- (2-hydroxyethyl)-N- (phenylmethyl)-3- pyridinecarboxamide X . C ; m d Chemical Structure,- m N r 122 2- [3-chloro-5- [ [ (2- 608 ho hydroxyethyl) (phenylmethyl) amin N H cl o] sulfonyl]-2-pyridinyl]-N- (10, 11- dihydro SH N :) Z 0 dihydro-5H- dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarbothioamide 123 2- [3-chloro-5- [ [ (2- 592 Ha hydroxyethyl) (phenylmethyl) amin N H C o] sulfonyl]-2-pyridinyl]-N- (10, 11- zozo dihydro-SH- dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarboxamide 124 2- [3-chloro-5- [ [ [ (1-ethyl-2- 585 pyrrolidinyl) methyl] amino] sulfony -N \ t]-2-pyridinyll-N- [7-ethenyl-8, 9- s N s=o dihydro-6- [ (lZ)-l-propenyl]-5H- benzocyclohepten-5-yl]- hydrazinecarbothioamide 125 CNNb 5-chloro-6-[2-[[(9-fluoro-6, 11-568 H dihydro-5H- N H. CI -N \ benzo [5, 6] cyclohepta [ 1, 2- S H \ O _ _ c] pyndm 11 yl) amino] thioxomethyl] hydrazino] - N [2- (1-methyl-2- pyrrolidinyl) ethyl]- 3- pyridinecarboxamide moWA h. C ; m C uc. r ; : t he c 1 N. me, Mass- 126 '/Fr5-chloro-6- [2- [ [ (9-fluoro-6, l 1-"591 dihydro-SH N Hcl /-N \-N-\, OH benzo [5, 6] cyclohepta [1, 2- s N o c] pyndm-11- F yl) amino] thioxomethyl] hydrazino] - N (2-hydroxyethyl)-N- (phenylmethyl)-3- pyridinecarboxamide 127 N [ [5-chloro-6- [2-jj (6, 11- 556 s H dihydrodibenzo [b, e] thiepin-l l- N H CI yl) amino] thioxomethyl] hydrazino] l S H /o > H o/-3-pyridinyl] carbonyl]-N-methyl- glycine, ethyl ester 128 (\ N-[[5-chloro-6-[2-[[(6, 11-dihydro-539 AN 5H-benzo [5, 6] cyclohepta [1, 2- N ici cpYndin-11- I S H I N o yl) amino] thioxomethyl] hydrazino] -3-pyridinyl] carbonyl]-N-methyl- glycine, ethyl ester 129 3 5-chloro-N-[2-555 s H (diethylamino) ethyl]-6-[2-[[(6, 11- N hui HN dihydrodibenzo [b, e] thiepin-1l- S H o yl) amino] thioxomethyl] hydrazino] - 3-pyridinecarboxamide Ma d : :. :. Cheucal- : Str iir l. w " Che icaI Name c as. : * t P 130 "5-chloro-7V- [ (l-ethyl-2-'568 H pyrrolidinyl) methyl]-6- [2- [ [ (9- N H ci < kN\ Nt) fluoro-6, 11-dihydro-5H- Y\ N N Nô benz [5, 6] cyclohepta [1, 2- c] pyridin-11- yl) amino] thioxomethyl] hydrazino] -3-pyridinecarboxamide 131 ; S-chloro-N [2-556 N ci (diethylamino) ethyl]-6- [2- [j (9- N cri HN fluoro-6, 11-dihydro-SH H benzo [5, 6] cyclohepta [1, 2- F c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinecarboxamide 132 N 5-chloro-A'- [2- 538 (diethylamino) ethyl]-6- [2- [ [ (6, 11- N H CI > > NX HN~N dihydro-5H- s N N o benzoj5, 6] cyclohepta [1, 2- c] pyridin-11- yl) amino] thioxomethyl] hydrazino] - 3-pyridinecarboxamide 133 C ENN 5-chloro-6-[2-[[(6, 11-dihydro-5H-536 benzo [5, 6] cyclohepta [1, 2- clpyridin-I I- N H N o yl) amino] thioxomethyl] hydrazino) - N [2- (1-pyrrolidinyl) ethyl]- 3- pyridinecarboxamide ,.,"t.-.. k x_ , f T... :.. t.., Y. y. 5 : : '.' d s :,. r.. V 4 ur, he ic 1- a-in C :, chennica Struc d, e a-G.. ncl a. N e as :,. :- , a : 1"Y. s f. r y i : t, a ?, z... a-r x, <.. 1Ty t r o 139 5-chloro-6- [2- [ [ (9-iluoro-6, ll- 541 H dihydro-5H- N C1 zon \ N benzo [5, 6] cyclohepta [1, 2- "N-' o IpYi'idin-11- F yl) amino] thioxomethyl] hydrazino] - N (tetrahydro-2-oxo-3-furanyl)- 3-pyridinecarboxamide 140 CN 5-chloro-6-[2-[[(6, 11-dihydro-5H-550 benzo [S, 6] cyclohepta [1, 2- ci c] pyridin-11- S H. N/ o C yl) amino] thioxomethyl] hydrazmo] -N- [ (I-ethyl-2- pyrrolidinyl) methyl]- 3- pyridinecarboxamide 141/"5-chloro-6- [2- [ [ (6, 11-dihydro-SH-523 H benzo [5, 6] cyclohepta [1, 2- N cri c pyridin-11- s N-J o yl) amino] thioxomethyl) hydrazino] - N (tetrahydro-2-oxo-3-furanyl)- 3-pyridinecarboxamide 142 CN\8 (5-chloro-6-[2-[[(6, 11-dihydro-5H-573 H benzo [5, 6] cyclohepta [1, 2- N H CI N c] pyridin-11- S H ! N o yl) amino] thioxomethyl] hydrazino] - N (2-hydroxyethyl)-N (phenylmethyl)-3- pyridinecarboxamide Wmm g zip d r,'enixca . T u, : : N, s e° ic 1 a' Cin Ch. 1 St uc : : e, - : : Gh,. m.. ., me : 11Ta55 : S S 3 . . tt n r.. a : : i s r-js s ;,, : u e + t eC." 143 y" 5-chloro-6- [2- [ [ (9-fluoro-6, ll- 597 dihydro-SH N H Cl H 1., rv N benzo [5, 6] cyclohepta [1, 2- /S N/ wF N O tX capyridin-11- N yl) aminolthioxomethyljhydrazino] - N [3- (4-methyl-1- piperazinyl) propyl]- 3- pyridinecarboxamide 144 _ _ 5-chloro-6-[2-[[(6, 11 dihydro-5H-579 benzo [5, 6] cyclohepta [1, 2- N H cl H c] pyridin-11- s N o 9 H J 8 yl) aminoathioxomethyl] hydrazino] - N [3- (4-methyl-1- piperazinyl) propyl]- 3- pyridinecarboxamide 145 N 5-chloro-6- [2- [ [ (9-fluoro-6, 1 1-576 dihydro-5H N H CI -N N benzo [5, 6] cyclohepta [1, 2- s . N O c] pyridin-l l- yl) amino] thioxomethyl] hydrazino] -N-methyl-N-[2-(2- pyridinyl) ethyl]- 3- pyridinecarboxamide 146 (aX Nx HN 5-chloro-N-[[(24-1-ethyl-2-557 pyrrolidinyl] methyl]-6- [2- [ [ [4- (4- propylcyclohexyl) phenyl] amino] th ioxomethyl] hydrazino]- 3- pyridinecarboxamide Wm m ' , Cna d : ., C. hemicaL St uctuive C eriuca-a P x", f : N me lIass : =-. : 147 C HN 5-chloro-N-[[(2R)-l-ethyl-2-0557 /-/. \==/\H) =. -, H pyrrolidinyl] methyl] propylcyclohexyl) phenyl] amino] th ioxomethyl] hydrazino]- 3- pyridinecarboxamide . 148 V-5-chloro-6- [2- [ [ (9-chloro-6, l 1-'607 dihydro-5H N H CI --N N'pH benzo [5, 6] cyclohepta [1, 2- s N N H c] pyndm-11- ci yl) amino] thioxomethyl] hydrazino] - N (2-hydroxyethyl)-N (phenylmethyl)-3- pyridinecarboxamide 149 N 5-chloro-6- [2- [ [ (9-fluoro-6, 1 I-554 dihydro-SH N ci -M, N HIN-NND benzo [5, 6] cyclohepta [1, 2- X c] pyridin-11- F yl) amino] thioxomethyl] hydrazino] - N [2- (1-pyrrolidinyl) ethyl]- 3- pyridinecarboxamide 150, 5NNb S-chloro-6-[2-[[(6, 11-dihydro-SH-558 AN H Cl \ N benzo [5, 6] cyclohepta [1, 2- H -N N , pyridin-11- S N H N o yl) amino] thioxomethyl] hydrazino] -N-methyl-N-[2-(2- pyridinyl) ethyl]- 3- pyridinecarboxamide X ". n, :. a Czn d, : xChemi-a t tc ure, a . 3... m a : E , 11 iy r a , n, e r ; 6 Y n., -,'. k. i' 151 \ 5-chloro-6- [2- [ [ (6, ll- 596 s H dihydrodibenzo [b, e] thiepin-11- N H ct H N yl) amino] thioxomethyl] hydrazino] /S H / H tNz-N-[3-(4-methyl-1- N . piperazinyl) propyl]-3- pyridinecarboxamide 152 (HN 5-chloro-N-[2-(1-methyl-2-557 (-"")"")-=\"''\ pyrrolidinyl) ethyl]-6- [2- [ [ [4- (4- . propylcyclohexyl) phenyl] amino] th ioxomethyl] hydrazino]- 3- pyridinecarboxamide 153-/.... yH, 2- [3-chloro-5- [ [ [ (l-ethyl-2-593 ci pyrrolidinyl) methyl] amino] sulfony 1]-2-pyridinyl]-N [4- (4- propylcyclohexyl) phenyl]- hydrazinecarbothioamide 154 ¢). 5-chloro-6-[2-[[(6, 11-567 s H dihydrodibenzo [b, e] thiepin-11- N ci H N---/'--C yl) amino] thioxomethyl] hydrazino] s N N \J'/ -- [2- (l-methyl-2- pyrrolidinyl) ethyl]- 3- pyridinecarboxamide 155 n 5-chloro-6-[2-[[(6, 11-567 H dihydrodibenzo [b, e] thiepin-l 1- N H ci H yl) amino] thioxomethyl] hydrazino] s N \ N No-N- [ (I-ethyl-2- pyrrolidinyl) methyl]- 3- pyridinecarboxamide dy ! ; : 5"Y f'. . F, 5 1 m n. ;. 4 1. f ., M f tvF., :., ; LIS :.. u Crri d s,, : , . ezxucal Sructu e. ; . r, ;. k , r, t, = -. r kroy . tu... fi ;. : s , Yig6 '/ [. 5-chloro-6- [2- [ [ (9-chloro-6, 11- 613 dihydro-SH meX N H CI H (XN H Cl dlhydrO-5X-. benzo [5, 6] cyclohepta [1, 2- 5 H ci N 0 clpyridin-11- ce yl) aminolthioxomethyllhydrazinol - N [3- (4-methyl-1- piperazinyl) propyl]- 3- pyridinecarboxamide 157/5-chloro-6- [2- [ [ (6, 1 l-dihydro-6-603 0 o S1 methyl-5, 5- N//H Ci N\/7 dioxidodibenzo [cf] [1, 2] thiazepin- s N---\\ N O yl) amino] thioxomethyl] hydrazino] ..-N-(tetrahydro-2-oxo-3-thienyl)- 3-pyridinecarboxamide 158 ci 5-chloro-N- [ (I-ethyl-2- 557 HN pyrrolidinyl) methyl]-6- [2- [ [ [4- (4- propylcyclohexyl) phenyl] amino] th ioxomethyl] hydrazino]- 3- pyridinecarboxamide 159 C \ 5-chloro-6-[2-[[(6, 11-dihydro-SH-550 benzo [5, 6] cyclohepta [1, 2- 0--C c] pyridin- I I- rv N-. I H amino thioxometh 1 h drazmo N o Y) Y Y - N [2- (1-methyl-2- pyrrolidinyl) ethyl]- 3- pyridinecarboxamide M, z ; : : Cm d'-. CheniacalStr. uc ure . : , _. t., h mac 1 m Mas" : ; r y. ,,. a t v t r f , s, S7n-1EC : ; e 160 y5-chloro-6- [2- [ [ (6, ll-'553 s H dihydrodibenzo [b, e] thiepin- 11- N-hui t HN''N'J yl) amino] thioxomethyl] hydrazino] s Hz N O-N-[2-(l-pyrrolidinyl) ethyl]-3- pyridinecarboxamide 161 2- [3-chloro-5- [ [ [ (I-ethyl-2- 569 H pyrrolidinyl) methyl] amino] sulfony N Hci H >-N \, N 1]-2-pyhdirlyl]-N- (l 0, 11-dihydro- I O H \ Sv/ o o ów \ 5H-dibenzo [a, d] cyclohepten-5-yl)- hydrazinecarboxamide 162 5-chloro-N-[(3R)-1-methyl-2-oxo-549 oNl, 3-pyrrolidinyl]-6- [2- [ [ [ (1R, 2R)- ci 1, 2, 3, 4-tetrahydro-2-phenyl-1- s N o naphthalenyl] amino] thioxomethyl] hydrazino]-3- pyridinecarboxamide 163 ; 5-chloro-6- [2- [ [ (9-chloro-6, 11- 584 H dihydro-5H- -N \ IN benzo [5, 6] cyclohepta [1, 2- N N zu N o c] pyndm-1l- C) yl) amino] thioxomethyl] hydrazino] - N [2- (1-methyl-2- pyrrolidinyl) ethyl]- 3- pyridinecarboxamide 164 2- [3-chloro-5- [ [ (2- 582 ci hydroxyethyl) (phenylmethyl) amin N H CI 0\, NOH o] sulfonyl]-2-pyridinyl]-1ç- I S H \ I Sv N 0 (diphenylmethyl)- hydrazinecarbothioamide . :. : F .,, y 'ei - : ; su :. , tu ; re : Gh°. ical.. a : u C : m. I d'<. Y. Cheiiucal : S ru m_ N me , :. : Mass : : 1655-chloro-6- [2- [ [ (6, ll-dihydro-6-'600 o I methyl-5, 5- I N ci N/-0\-H dioxidodibenzo [cA [1, 2] azepin- s N- N D yl) amino] thioxomethyl] hydrazino] - N (1-methyl-2-oxo-3- pyrrolidinyl)-3- pyridinecarboxamide
It will be understood that when compounds of the present invention contain one or more chiral centers, the compounds of the invention may exist in, and be isolated as, enantiomeric or diastereomeric forms, or as a racemic mixture. The present invention includes any possible enantiomers, diastereomers, racemates or mixtures thereof, of a compound of Formula I or II. The optically active forms of the compound of the invention may be prepared, for example, by chiral chromatographic separation of a racemate, by synthesis from optically active starting materials or by asymmetric synthesis based on the procedures described thereafter.
It will also be appreciated that certain compounds of the present invention may exist as geometrical isomers, for example E and Z isomers of alkenes. The present invention includes any geometrical isomer of a compound of Formula I or II. It will further be understood that the present invention encompasses tautomers of the compounds of the formula I or II.
It will also be understood that certain compounds of the present invention may exist in solvated, for example hydrated, as well as unsolvated forms. It will further be understood that the present invention encompasses all such solvated forms of the compounds of the formula I or II.
Within the scope of the invention are also salts of the compounds of the formula I or II. Generally, pharmaceutically acceptable salts of compounds of the present invention may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound, for example an alkyl amine with a suitable acid, for example, HCl or acetic acid, to afford a physiologically acceptable anion. It may also be
possible to make a corresponding alkali metal (such as sodium, potassium, or lithium) or an alkaline earth metal (such as a calcium) salt by treating a compound of the present invention having a suitably acidic proton, such as a carboxylic acid or a phenol with one equivalent of an alkali metal or alkaline earth metal hydroxide or alkoxide (such as the ethoxide or methoxide), or a suitably basic organic amine (such as choline or meglumine) in an aqueous medium, followed by conventional purification techniques.
In one embodiment, the compound of formula I or II above may be converted to a pharmaceutically acceptable salt or solvate thereof, particularly, an acid addition salt such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, methanesulphonate orp-toluenesulphonate.
The novel compounds of the present invention are useful in therapy, especially for the treatment of various pain conditions such as chronic pain, neuropathic pain, acute pain, cancer pain, pain caused by rheumatoid arthritis, migraine, visceral pain etc. This list should however not be interpreted as exhaustive.
Compounds of the invention are useful in disease states where degeneration or dysfunction of Bradykinin receptors is present or implicated in that paradigm. This may involve the use of isotopically labelled versions of the compounds of the invention in diagnostic techniques and imaging applications such as positron emission tomography (PET).
Compounds of the invention are useful for the treatment of septic shock, pancreatitis, edema, rhinitis, asthma, colitis, arthritis, hepatorenal syndrome, cancer, (including but not restricted to SCLC, prostrate cancer), bacterial and viral infections, ulcerative colitis, and Alzheimer's Disease.
Compounds of the invention are useful as an analgesic agent for use during general anaesthesia and monitored anaesthesia care. Combinations of agents with different properties are often used to achieve a balance of effects needed to maintain the anaesthetic state (e. g. amnesia, analgesia, muscle relaxation and sedation). Included in this combination are inhaled anaesthetics, hypnotics, anxiolytics, neuromuscular blockers and opioids.
Also within the scope of the invention is the use of any of the compounds according to the formula I or II above, for the manufacture of a medicament for the treatment of any of the conditions discussed above.
A further aspect of the invention is a method for the treatment of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to the formula I or II above, is administered to a patient in need of such treatment.
Thus, the invention provides a compound of formula I or II, or pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined for use in therapy.
In a further aspect, the present invention provides the use of a compound of formula I or II, or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined in the manufacture of a medicament for use in therapy.
In the context of the present specification, the term"therapy"also includes "prophylaxis"unless there are specific indications to the contrary. The term"therapeutic" and"therapeutically"should be contrued accordingly. The term"therapy"within the context of the present invention further encompasses to administer an effective amount of a compound of the present invention, to mitigate either a pre-existing disease state, acute or chronic, or a recurring condition. This definition also encompasses prophylactic therapies for prevention of recurring conditions and continued therapy for chronic disorders.
The compounds of the present invention are useful in therapy, especially for the therapy of various pain conditions including, but not limited to: acute pain, chronic pain, neuropathic pain, acute pain, back pain, cancer pain, and visceral pain.
In use for therapy in a warm-blooded animal such as a human, the compound of the invention may be administered in the form of a conventional pharmaceutical composition by any route including orally, intramuscularly, subcutaneously, topically, intranasally, intraperitoneally, intrathoracially, intravenously, epidurally, intrathecally, intracerebroventricularly and by injection into the joints.
In one embodiment of the invention, the route of administration may be orally, intravenously or intramuscularly.
The dosage will depend on the route of administration, the severity of the disease, age and weight of the patient and other factors normally considered by the attending physician, when determining the individual regimen and dosage level at the most appropriate for a particular patient.
For preparing pharmaceutical compositions from the compounds of this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form
preparations include powders, tablets, dispersible granules, capsules, cachets, and suppositories.
A solid carrier can be one or more substances, which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, or table disintegrating agents; it can also be an encapsulating material.
In powders, the carrier is a finely divided solid, which is in a mixture with the finely divided compound of the invention, or the active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.
For preparing suppository compositions, a low-melting wax such as a mixture of fatty acid glycerides and cocoa butter is first melted and the active ingredient is dispersed therein by, for example, stirring. The molten homogeneous mixture in then poured into convenient sized moulds and allowed to cool and solidify.
Suitable carriers are magnesium carbonate, magnesium stearate, talc, lactose, sugar, pectin, dextrin, starch, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, a low-melting wax, cocoa butter, and the like.
The term composition is also intended to include the formulation of the active component with encapsulating material as a carrier providing a capsule in which the active component (with or without other carriers) is surrounded by a carrier which is thus in association with it. Similarly, cachets are included.
Tablets, powders, cachets, and capsules can be used as solid dosage forms suitable for oral administration.
Liquid form compositions include solutions, suspensions, and emulsions. For example, sterile water or water propylene glycol solutions of the active compounds may be liquid preparations suitable for parenteral administration. Liquid compositions can also be formulated in solution in aqueous polyethylene glycol solution.
Aqueous solutions for oral administration can be prepared by dissolving the active component in water and adding suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous suspensions for oral use can be made by dispersing the finely divided active component in water together with a viscous material such as natural synthetic gums, resins, methyl cellulose, sodium carboxymethyl cellulose, and other suspending agents known to the pharmaceutical formulation art.
Depending on the mode of administration, the pharmaceutical composition will preferably include from 0.05% to 99% w (per cent by weight), more preferably from 0.10 to 50% w, of the compound of the invention, all percentages by weight being based on total composition.
A therapeutically effective amount for the practice of the present invention may be determined, by the use of known criteria including the age, weight and response of the individual patient, and interpreted within the context of the disease which is being treated or which is being prevented, by one of ordinary skills in the art.
Within the scope of the invention is the use of any compound of formula I or II as defined above for the manufacture of a medicament.
Also within the scope of the invention is the use of any compound of formula I or II for the manufacture of a medicament for the therapy of pain.
Additionally provided is the use of any compound according to Formula I or II for the manufacture of a medicament for the therapy of various pain conditions including, but not limited to: acute pain, chronic pain, neuropathic pain, acute pain, back pain, cancer pain, and visceral pain.
A further aspect of the invention is a method for therapy of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to the formula I or II above, is administered to a patient in need of such therapy.
Additionally, there is provided a pharmaceutical composition comprising a compound of Formula I or II, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier.
Particularly, there is provided a pharmaceutical composition comprising a compound of Formula I or II, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier for therapy, more particularly for therapy of pain.
Further, there is provided a pharmaceutical composition comprising a compound of Formula I or II, or a pharmaceutically acceptable salt thereof, in association with a pharmaceutically acceptable carrier use in any of the conditions discussed above.
In a further aspect, the present invention provides a method of preparing a compound of formula I or II.
Compounds of formula I or II have been prepared as single compound syntheses and/or using parallel synthetic protocols.
In one embodiment, the present invention provides a process for preparing compounds of Formula I wherein X is represented by formula (i) or (ii), comprising reacting a compound of general formula IV, wherein G is CH or N and R3 is halogen, with an isocyanate (Y-NCO) or thioisocyanate (Y-NCS), wherein Y is as defined above; to give a compound of general formula V, wherein T is S or O ; which is further coupled with a primary or secondary amine HNRIR2, wherein R'and R2 are as defined above, using a standard amide coupling reagent such as HATU and an acid scavenger such as diisopropylethyl amine (DIPEA), to yield a compound of formula I, wherein W is-C (=O)-.
In another embodiment, the present invention provides a method of preparing a compound of formula VI, wherein the method includes the step of reacting a compound of formula VII with a compound of Y-NCO or Y-NCS : to form the compound of formula VI:
wherein T is O or S; and W, Y, G, Rl and R2 are as defined above.
In an even further aspect, the present invention provides useful reaction intermediates Y-NCS, Y-NCO, and the compounds of formulas (V) and (VII), wherein W, Y, G, Rl and R2 are as defined above.
Compounds of the present invention may also be prepared by combinatorial methods.
General combinatorial protocol for plates : The corresponding acid (0.04 m in DMA, 0. 5 ml, 20 mol), 80 different amines (0. 5m in DMA, 50 pt1, 25 pmol), and DIPEA (1m in DMA, 50 p1, 50, umol) are successively distributed to a 96-well format plate, then HATU (0.25m in DMA, 100 u. I, 25 umol) is added to the wells. The plate is shaken overnight at room temperature and worked up by removing DMA under reduced pressure, and adding dichloromethane (500 1ll) to the wells, and washing with H20 (3 x 500 µl), then dichloromethane is evaporated in vacuo to provide a-10 mg per well plate with most of the compound purity in the range of 50-90%.
It will be understood by those of ordinary skill in the art that a chemical reaction which fails to efficiently yield the desired product within the context of a combinatorial protocol may nonetheless efficiently yield the desired product when the reaction is performed in a single reaction or parallel reaction format, without undue experimentation on the part of the chemist. In this regard, several of the compounds which were not prepared efficiently in the combinatorial array, were subsequently prepared in separate syntheses.
Particularly, the compounds of the present invention can be prepared according to the synthetic routes as exemplified in Schemes 1-9 and further detailed in the Examples, wherein Y and R'and R2 are as defined above.
Scheme 1 : Synthesis of semicarbazides and thiosemicarbazides for Examples 1-15, 18-21,24-25, 27-32,35, 38-39,41-43, 49:
0 0 ,, OH Solvent OH + or H H H, 2N, Y, z Y-NCS y Ny N z Y-NCS T Xi=H, a ; Y1=NH, NMe, CH2 Z'=N, CH 0 H HATU N R2 x N-R 2 HATU Y) Z T T=S, orO Scheme 2: Alternative Approach to the synthesis of semicarbazides and thiosemicarbazides for Examples 26,27, 38,40, 50 : Scheme 3: Synthesis of sulfonamide compounds for Examples 16, 17, 48: Scheme 4: Synthesis of reverse amide compounds for Examples 45-47:
11 0 ri 1-BOC20 R2 ci Cl Ny R -- -- ci N N tertiary mine C ; ruz R1 Y-NCO, R1 NH CI N'R2 Y-NCS H H C N R -nus yR Y H 0 microwave H2N>N) 0 y N N H T= O, or S T= 0, or S Scheme 5: Synthesis of benzyl ureas for Examples 11-14 : CI CN CtCCN C » CN C » o TFA C » CN cl c 2 3 V Br N (Bocj NH2 nez Nco p cone. HCI ci OH H H OH conc. 2, k healing r-O NH2. HCI 0 /1 C/Rz N 'N N \ I R' HATU O Scheme 6: Synthesis of pyridylmethyl ureas and pyridylmethyl thioureas for Examples 15,42, 43:
0 0 cl ci OMe NACN, DM, 4p cl OMe Rariey Ni, H2 cl OMe Cl EtCN, reflux NC MeOH NH2 Y-NEO cl Y-NCS OMe NaOH C I OH Y-NCS H H l H H l Solvent N I N' YN N Solvent T 0 0 T=O, orS hotu I I T Scheme 7. Synthesis of (aza) dibenzosuberane, dibenzothiepine thioisocyanate for Examples 20,22-50 R 40 R40 40 40 CS2. EDC, TEA , or 1/ 7 NHZOH. HCI w Zn, NH40Ac W77'CSCS2. NaHC03 NOH '" solvent, A NOH NH40H, ETOH NH2 Solvent 7 7 CH2, A S°2 'w \J Q' Y7-\y7 _ Y7 X7=CH or N Y7=CH or N Z7=CH or N R40=H, F, Me, Cl Scheme 8. Alternative approach to substituted dibenzosuberane thioisocyanate for Examples 19 and 21: . R48 = H, halogen, alkyl, aryl Scheme 9. Synthesis of azabenzosuberane urea and semicarbazide for compounds 22-23,33-34, 36-37 and 44: R R R rusa CDI N H H 0 H 2 N ci R so _ Souvent X X I I \Ys_ Ys_Zs X\Ys_Zs
X9 = CH, or N ; Y9=CHorN ; Z9 = CH or N ; R49 = H, halogen, alkyl, or aryl ; W9 = NH or CH2 R50 = H, halogen, alkyl, or aryl.
Biological Evaluation I. B2 bradykinin A. Human Bradvkinin B2 (hB2) receptor expression and membrane preparation The cloned human Bradykinin B2 (hB2) receptor in the pCIN vector was purchased from Receptor Biology. The hB2 receptor was stably transfected into HEK 293 S cells and a clonal cell line was generated. Cells were grown in T-flasks with DMEM culture media containing 10% FBS, 2 mM glutamine, 600ug/ml neomycin and an antibiotic cocktail (100 IU penicillin, 100pg/ml streptomycin, 0. 25ug/ml amphotericin B). Membranes, expressing the hB2 receptor, were prepared from this cell line according this protocol: Cells are harvested at 1 to 1.2 million cells/ml, pelleted, and resuspended in ice-cold lysis buffer (50 mM Tris, pH 7.0, 2.5 mM EDTA, with PMSF added just prior to use to 0.5 mM from a 0.5 M stock in DMSO. After lysis on ice for 15 min, the cells are homogenized with a polytron for 10 sec. The suspension is spun at 1000g for 10 min at
4°C. The supernatant is saved on ice and the pellets resuspended and spun as before. The supernatants from both spins are combined and spun at 46, 000g for 10-30 min. The pellets are resuspended in cold Tris buffer (50 mM Tris/Cl, pH 7.0) at a dilution of 0. 2-1 ml per 40 million cells and spun again. The final pellets are resuspended in membrane buffer (50 mM Tris, 0.32 M sucrose, pH 7.0). Aliquots are frozen in dry ice/ethanol and stored at-70°C until use. The protein concentrations are determined by a modified Lowry with SDS.
B. hB2 receptor binding Membranes expressing the hB2 receptor are thawed at 37°C, passed 3 times through a 25- gauge blunt-end needle, diluted in the bradykinin binding buffer (50 mM Tris, 3mM MgCl2, and 1 mg/ml BSA, pH 7.4, 0.02 mg/ml Phenanthroline, 0.25 mg/ml Pefabloc) and 80 uL aliquots containing the appropriate amount of protein (final concentration of 0. 25pg'ml) are distributed in 96-well polystyrene plates (Treff Lab). The ICso of compounds are evaluated from 10-point dose-response curves, where the serial dilutions are done on a final volume of 1501lu, with 70StL of 125I-Desamino-TyrHOE140 (Kd=0. 05) at 50,000 to 60,000 dpm per well (0.03-0. 04nM) in a final volume of 300, u1.
The total and non-specific binding are determined in the absence and presence of 0.1 M (150µL) of Bradykinin respectively. The plates are vortexed and incubated for 60 minutes at room temperature, filtered through Unifilters-96 GF/B (Canberra Packard), which were presoaked in 0.1 % polyethyleneimine, with a harvester using 3ml of wash buffer (50 mM Tris, pH 7.0, 3mM MgCl2). The filters are dried for 1 hour at 55°C. The radioactivity (cpm) is counted in a TopCount (Canberra Packard) after adding 65 gl/well of MS-20 scintillation liquid (Canberra Packard). Compounds of the present invention have demonstrated hB2 receptor binding at concentrations less than 10 0 pLM.
Based on the above assays, the dissociation constant (Ki) for a particular compound of the invention towards a particular receptor is determined using the following equation: Ki = IC50/(1+[rad]/Kd), Wherein IC50 is the concentration of the compound of the invention at which 50% displacement has been observed; [rad] is a standard or reference radioactive ligand concentration at that moment; and
Kd is the dissociation constant of the radioactive ligand towards the particular receptor.
GTP [Y] 35S binding experiments on Bradykinin (B2) receptors A. General Information The procedures below describe how to perform and interpret GTP [y] 35s binding experiments designed to determine the activity of new compounds on the human B2 receptor.
B. General procedure of the assay Human Bradykinin-2 GTP [y] 35S Binding Human Bradykinin-2 membranes (hB2 293s) are thawed at 37°C, passed 3 times through a 25-gauge blunt-end needle and diluted in the GTPyS binding buffer for the assay (50 mM Hepes, pH 7.4 ; 200 mM NaCl ; 1 mM EDTA; 5 mM MgCl2. To this added freshly prepared 1 mM DTT, 0.5% BSA, 1 pM GDP. The EC50 and Emax of compounds are evaluated from 10- point dose-response curves done in 300gui with the appropriate amount of membrane protein and 100,000-120, 000 dpm of GTPy35S per well (0.11-0. 14 nM). Bradykinin (1-9) is used as the standard agonist at hB2. The ranges of concentrations tested should include a maximal concentration off. lux bradykinin in order to establish the Emax.
The plates are vortexed and incubated for 60 minutes at room temperature, filtered on GF/B Unifilters (presoaked in water) with the Packard harvester using 4 ml/well of wash buffer (50 mM Tris, 5 mM MgCl2, 50 mM NaCl, pH 7.0), minimum. The filters are dried for 1 hour at 55°C. The radioactivity (cpm) is counted in a TopCount (Packard) after adding 65 ullwell of MS-20 scintillation liquid.
Antagonist reversal studies are done in a similar manner except that the compound dose-response curve's are performed in the presence of a constant concentration of agonist (approx. 80% bradykinin EmaX ; ~ 5 nM). A standard B2 Antagonist is used as the reference antagonist at hB2. The ranges of antagonist concentrations tested should include a maximal concentration of 3, uM of the standard B2 Antagonist in order to establish the maximal displacement (Dmax) C. Radioligand : Preparation of GTPlrl35S
GTP [y] 35S is acquired from Perkin-Elmer (250, pCi/20, ul). It is diluted from with 10 mM DTT, 50 mM Tris, pH 7 (dilute in 2 ml, 1. 0 mCI/20µ). Sonicate the solution, filter through a 0. 45 µm filter, and freeze aliquots at-70°C. For the experiment, use 3 nM dilution of this tracer in the GTP binding buffer.
D. Data analysis The EC50 and Emax of compounds are evaluated from 10-point dose-response curves done in 300go with the appropriate amount of membrane protein and GTPγ35S per well and are calculated in Activity base with ExcelFit. The basal and maximal stimulated binding are determined in absence and presence of standard reference compounds, respectively.
The stimulation (Stim) in the presence of compounds is expressed as the percentage of DmaX of the reference antagonist. Values of IC5o, Ki'and DmaX for ligands capable of competing for agonist stimulated binding are calculated in Activity Base.
Mean S. E. M. values of IC5o, Ki'and % Dmax are reported for ligands tested in at least three dose-response curves.
Biological data for certain compounds of the invention are listed in Table 2 below.
Table 2 Compound Nos. Ki (hB2) (nM) 1-165 5-5000 EXAMPLES The invention will further be described in more detail by the following Examples which describe methods whereby compounds of the present invention may be prepared, purified, analyzed and tested, and which are not to be construed as limiting the invention.
Example 1: 5-Chloro-6-[2-[[(10,11-dihydro-5H-dibenzo [a, d] cyclohepten-5- yl) amino]-thioxomethyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-thienyl]-3- pyridinecarboxamide.
1A : 10, 11-dihydro-5-isothiocyanato-SH-dibenzoEatdlcycloheptene.
CS2 (51.7 mL, 860 mmol) and EDC (30.2 g, 157.5 mmol) was added to the suspension of 10, 11-dihydro-5H-dibenzo [a, d] cyclohepten-5-amine (30.0 g, 143.3 mmol) (prepared according to P. Melloni et al J. Med. Chem. 1979,22, 183-191; WO 0160826) in Et2O (900 mL) at-10 °C. The reaction mixture was stirred at this temperature for 15 min, then Et3N (22 mL, 157 mmol) was added at such rate that the temperature was maintained between-10 and-5 °C. The resulting mixture was stirred at-10 °C for 3 hrs, and then room temperature overnight. The reaction mixture was then filtered and the filtrate was concentrated. The residue was dissolved in EtOAc (600 mL), and washed sequentially with 5% HCl (50 mL), H20 (50 mL), 5% aq. NaHC03 (50 mL), and brine (2x50 mL). The organic phase was dried over MgS04, filtered and concentrated in vacuo.
The residue was recrystallized in hexanes/EtOAc to produce the title compound (24.7 g, 69%).
1B : 5-chloro-6-E2-Er (10, 11-dihydro-5H-dibenzoFadlcyclohePten-5-yl) aminolthioxo- methylLvdrazino]pyridinecarboxylic acid. 0 o/\/\ ci i \ pH 2 eq. pyridine (S--N HN XOH H2Ns + u DMA 41 S H N N DMA H' /_
A mixture of 5-chloro-6-hydrazino-3-pyridinecarboxylic acid (1. 91 g, 10.02 mmol) (prepared from commercial 5,6-dichloronicotinic acid and hydrazine according to: Graf J. Prakt. Chem. 1933,138, 244-256, which is incorporated by reference herein for its disclosure in the preparation of 5-chloro-6-hydrazino-3-pyridinecarboxylic acid) and 10, 11-dihydro-5-isothiocyanato- 5H-dibenzo[a, d] cycloheptene (2.57 g, 10.2 mmol) in DMA (80 mL) containing 1.7 mL of pyridine was stirred at room temperature overnight.
The solvent was removed in vacuo, and the residue was triturated with Et20 (30 mL). The white solid was collected and dried in vacuo to afford a white solid (5.30 g, quantitative) as a 1: 1 complex of the title compound with DMA. 1HMR (DMSO-d6): 8 13.00 (s, 1H), 9.67 (s, 1H), 9.41 (s, 1H), 8.93 d, J=8.8 Hz, 1H), 8.57 (s, 1H), 8.03 (s, 1H), 7. 52 (brs, 1H), 7.27 (d, J = 6Hz, 2H), 7.09 (br, 4H), 7.01 (br, 2H), 3.24 (m, 2H), 2.94 (m, 2H), 2. 91 (s, 3H, DMA), 2.75 (s, 3H, DMA), 1.92 (s, 3H, DMA) ppm. MS (ESI) (M+1) += 439.
A small fraction of the product was recrystallized in EtOH to give DMA-free title compound.'HNMR (DMSO-d6): 5 13.00 (s, 1H), 9.67 (s, 1H), 9.41 (s, 1H), 8.93 d, J=8.8 Hz, 1H), 8.57 (s, 1H), 8.03 (s, 1H), 7.52 (brs, 1H), 7.27 (d, J = 6Hz, 2H), 7.09 (br, 4H), 7.01 (br, 2H), 3.24 (m, 2H), 2.94 (m, 2H) ppm.
1 C : 5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl)amino]- thioxomethyl]hydrazino]-N-[(3R)-tetrahydro-2-oxo-3-thienyl]- 3-pyridinecarboxamide.
HATU (190 mg, 0.50 mmol) was added to a mixture of 5-chloro-6- [2- [ [ (10, 11- dihydro-5H-dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino] -3- pyridinecarboxylic acid. DMA (180 mg, 0.34 mmol), D-homocysteine thiolactone hydrochloride (65 mg, 0.42 mmol) and DIPEA (0.30 mL) in DMA (5 mL) at room temperature. The reaction mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure. Water (5 mL) was added to the residue and the solid was collected and dried in vacuo to give crude product (215 mg). The product was recrystallized twice in EtOH to provide the title compound (60 mg, 33%) as light gray
solid. Me +23. 7° (c 0.23, DMA).'HNMR (DMSO-d6): 8 9.64 (s, 1H), 9.27 (s, 1H), 8. 89 (brs, 1H), 8.69 (d, J = 6.8 Hz, 1H), 8.54 (s, 1H), 8. 10 (s, 1H), 7.48 (br, 1H), 7.26 (d, J = 6.4 Hz, 2H), 7. 20-6.90 (m, 6H), 4. 81 (m, 1H), 3. 43 (m, 1H), 3.32 (m, 1H), 2.93 (m, 1H), 2.52 (m, 4H), 2.23 (m, 1H) ppm. MS (ESI) (M+1) + = 538. Anal. Calcd for C26H24C1N502S2. H20 : C, 56.16 ; H, 4.67 ; N, 12.59. Found: C, 56.33 ; H, 4. 39 ; N, 12.41.
Example 2: 5-Chloro-6-[2-[[(10,11-dihydro-5H-dibenzo [a, d] cyclohepten-5- yl) amino]-thioxomethyl] hydrazino]-N-[(3S)-tetrahydro-2-oxo-3-thienyl]-3- pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1C: the title compound (110 mg, 23%) was prepared from 5-chloro-6-[2-[[(10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino]-3-pyridinecarboxylic acid. DMA (460 mg) and L-homocysteine thiolactone hydrochloride (180 mg).
'HNMR (DMSO-d6): 8 9.64 (s, 1H), 9.27 (s, 1H),. 8.89 (brs, 1H), 8.69 (d, J = 6.8 Hz, 1H), 8.54 (s, 1H), 8.10 (s, 1H), 7.48 (br, 1H), 7.26 (d, J = 6.4 Hz, 2H), 7.20-6. 90 (m, 6H), 4.81 (m, 1H), 3.43 (m, 1H), 3.32 (m, 1H), 2.93 (m, 1H), 2.52 (m, 4H), 2.23 (m, IH) ppm.
MS (ESI) (M+1) + = 538. Anal. Calcd for C26H24ClN502S2. 1. 25H20: C, 55.71 ; H, 4. 76 ; N, 12.49. Found: C, 55.71 ; H, 4.40 ; N, 12.55. [α] D-22. 8° (c 0.52, DMA).
Example 3 : 5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) aminol thioxomethyl]-hydrazino]-N-(tetrahydro-2-oxo-3-furanyl)-3- pyridinecarboxamide.
Following general HATU coupling procedure of Example 1C : The title compound (50 mg, 55%) was obtained from 5-chloro-6- [2- [ [ (10, 11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino]-3-pyridinecarboxylic acid. DMA (92 mg, 0.175 mmol) and cc-amino-y-butyrolactone hydrobromide (40 mg, 0.22 mmol).'HNMR (DMSO-d6): 8 9.65 (9.29) (brs, 1H), 8.94 (br, 2H), 8.55 (s, 1H), 8.11 (s, 1H), 7.49 (br, 1H), 7.29 (br, 2H), 7.20-6. 90 (m, 7H), 4.80-4. 66 (m, 1H), 4.44-4. 36 (m, 1H), 4.30-4. 18 (m, 1H), 3.35-3. 16 (br, 2H), 3.02-2. 86 (br, 2H), 2.50-2. 40 (m, 1H), 2. 36-2.24 (m, 1H) ppm. MS (ESI) (M+1) += 522. Anal. Calcd for C26H24CIN503S. 1. 75H20 : C, 56.42 ; H, 5.01 ; N, 12.65. Found: C, 56.40 ; H, 4.72 ; N, 12.42.
Example 4: N-[[5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo [a, d] cyclohepten-5- yl) amino]-thioxomethyl] hydrazino]-3-pyridinyl] carbonyl]-N-methylglycine ethyl ester.
Following general procedure of HATU coupling of Example 1C : HATU (40 mg, 0.105 mmol) was added to a mixture of 5-chloro-6-[2-[[(10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino]-3-pyridinecarboxylic acid. DMA (50 mg, 0.095 mmol), sarcosine ethyl ester hydrochloride (17 mg, 1.10 mmol), and DIPEA (0.25 mmol) in DMA (4 mL) at room temperature. The reaction mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure.
Water (5 mL) was added to the residue and the solid was collected and dried'in vacuo to give crude product. The crude product was purified by flash chromatography on silica gel (EtOAc: CH2Cl2 1 : 4) to provide the title compound (30 mg, 59%). 1HNMR (DMSO-d6): 8 9.60 (9. 18) (br, 1H), 8.20 (8.05) (br, 1H), 7.79 (7.67) (br, 1H), 7.49 (br, 1H), 7.26 (br, 2H), 7.20-6. 90 (m, 7H), 4.16 (s, 2H), 4.10 (m, 2H), 3.05 (s, 3H), 2.94 (m, 4H), 1.18 (m, 3H) ppm. MS (ESI) (M + : 538.
Compound 5:5-chloro-6-[2-[[ (10, 11-dihydro-5H-dibenzola, d] cyclohepten-5- yl) amino] thioXómethyl] hydrazinol-N-(2-hydroxyethyl)-N-(phenylmethyl)-3- pyridinecarboxamide.
HATU (42 mg, 0.11 mmol) was added to a mixture of 5-chloro-6-[2-[[(10,11- dihydro-SH-dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino] -3- pyridinecarboxylic acid. DMA (53 mg, 0.10 mmol), N-benzylethanolamine (17 mg, 0.11 mmol), and DIPEA (39 mg, 0.3 mmol) in DMA (4 mL) at room temperature. The reaction mixture was stirred at room temperature for 2 h, worked up by adding H20 (10 mL), extracted with EtOAc (2x30 mL). The extracts were washed with H20 (10 mL), saturated NaHCO3 (5 mL), brine (5 mL), and dried over sodium sulfate. Removal of solvent gave a yellow semi-solid which was triturated with H20 to give a yellow solid.
The solid was collected and dried in vacuo and then purified by. preparative TLC plate (EtOAc: CH2Cl2 1 : 1) to give the title compound (12 mg, 21%).'HNMR (DMSO-d6) : 8 9.56 (9. 08) (s, 1H), 8.87 (br, 1H), 8.22 (brs, 1H), 7.88 (brs, 1H), 7.46 (br, 1H), 7.40-6. 80 (m, 14H), 4. 66 (s, 2H), 3.60-3. 40 (m, 2H), 3. 38-3. 10 (m, 4H, overlap with H20 in DMSO), 3.00-2. 80 (m, 2H) ppm. MS (ESI) (M+1) + : 572.
Example 6: 5-chloro-6- [2- [ [ (10, 11-dihydro-5H-dibenzo [a, d] cyclohepten-5- yl) amino] carbonyl]-hydrazino]-N- (tetrahydro-2-oxo-3-thienyl)-3- pyridinecarboxamide.
6A: 5-chloro-6-f2-ff ((10, 11-dihYdro-5H-dibenzo [a, dlcyclohepten-5-ylaminolcarbonyll- hydrazinol-3-pvridinecarboxylic acid.
A mixture of 5-chloro-6-hydrazino-3-pyridinecarboxylic acid (1.88 g, 10.01 mmol) and 10, 11-dihydro-5-isocyanato-5H-dibenzo[a,d]cycloheptene (2. 38 g, 10.11 mmol) (prepared according to: M. A. Davis et al J. Med. Chem. 1964,7, 88-94, which is incorporated by reference herein for its disclosure of the preparation of 10, 11-dihydro-5- isocyanato-5H-dibenzo [a, d] cycloheptene) and pyridine (1.65 mL, 20 nunot) in DMA (60 mL) was stirred at room temperature overnight. The solvent was removed in vacuo, and the residue was triturated with MeOH (3 mL) and washed with Et20 (50 mL) and dried to give a white solid (4.60 g, 90.4%) as a 1: 1 complex of the title compound and DMA.' IHNMR (DMSO-d6) : 8 9.01 (brs, 1H), 8. 48 (s, 1H), 7.95 (s, 1H), 7.94 (brs, 1H), 7.58- 7. 52 (m, 1H), 7.33 (d, J= 7.6 Hz, 2H), 7.18-7. 05 (m, 6H), 6.24 (br, 1H), 3.24-3. 04 (m, 4H), 2. 91 (s, 3H, DMA), 2.75 (s, 3H, DMA), 1.92 (s, 3H, DMA) ppm. MS (ESI) (M+1) + : 423.
6B: 5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl)amino]carbonyl]- hydrazinol-N-(tetrahydro-2-oxo-3-thienyl)-3-pyrìdinecarboxa mide.
Following general HATU coupling procedure of Example 1 C : HATU (76 mg, 0.20 mmol) was added to a mixture of 5-chloro-6-[2-[[(10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl]-hydrazino]-3-pyridinecarboxylic acid.
DMA complex (82 mg, 0.16 mmol), homocysteine thiolactone hydrochloride (31 mg, 0. 20 mmol), and DIPEA (0.15 mL) in DMA (4 mL) at room temperature. The reaction
mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure. Water (10 mL) was added to the residue and the solid was collected.
The product was recrystallized in EtOH and EtOAc to provide the title compound (54 mg, 64%). 1HNMR (DMSO-d6) : 8 8. 88 (7.92) (s, 1H), 8. 65 (d, J = 8.0 Hz, 1H), 8.47 (d, J = 1. 2 Hz, 1H), 8.05 (s, 1H), 7.60-7. 50 (m, 1H), 7.34 (d, J= 6. 0 Hz, 2H), 7.13 (br, 6H), 6.25 (br, 1H), 4.85-4. 76 (m, 1H), 3.48-3. 38 (m, 1H), 3.34-3. 26 (m, 1H, overlap with H20 in DMSO-d6), 3.22-3. 06 (m, 4H), 2.50-2. 40 (m, 1H, overlap with DMSO), 2.28-2. 16 (m, 1H) ppm. MS (ESI) (M+1) + : 522. Anal. Calcd for C26H24ClN503S. 0. 25lI20 : C, 59.30 ; H, 4.70 ; N, 13.29. Found: C, 59.39 ; H, 4.67 ; N, 13.19.
Example 7: N-[[5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepte n-5- yl) amino] carbonyl]-hydrazino]-3-pyridinyl] carbonyl]-N-methyl-glycine ethyl ester.
Following general HATU coupling procedure of Example 1 C : HATU (40 mg, 0. 11 mmol) was added to a mixture of 5-chloro-6-[2-[[(10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl]-hydrazino]-3-pyridinecarboxylic acid DMA complex (51 mg, 0.10 mmol), homocysteine thiolactone hydrochloride (17 mg, 0.11 mmol), and DIPEA (50 pL) in DMA (4 mL) at room temperature. The reaction mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure. Water (10 mL) was added to the residue and the solid was collected.
The crude product was purified by preparative TLC (MeOH: CH2Cl2 1: 9) to provide the title compound (15 mg, 29%). 1HNMR (DMSO-d6): 5 8.75 (s, 1H), 8.09 (s, 0. 75H), 7.95 (s, 0.25H), 7.86 (s, 1H), 7.71 (s, 0.75H), 7.57 (s, 0.25H), 7.51 (d, J = 8Hz, 1H), 7. 31 (d, J =7.2Hz, 2H), 7.18-7. 04 (m, 6H), 6.25 (s, 1H), 4.12 (s, 2H), 4.06 (q, J = 7.2Hz, 2H), 3.20- 285 (m, 7H), 1.25-1. 05 (m, 3H, combination of two sets of triplets from two rotamers).
MS (ESI) (M+1) + : 522.
Example 8 : 5-chloro-N-(tetrahydro-2-oxo-3-thienyl)-6-[2-[[[(1R*,2S*)-1, 2,3, 4- tetrahydro-2-phenyl-1-naphthalenyl] amino] thioxomethyl] hydrazino]-3- pyridinecarboxamide.
8A: (lR*, 2S*)-1, 2, 3, 4-tetrahydro-1-isothiocyanato-2-phenyl-naphthalene.
To the vigorously stirred reaction mixture of (lR*, 2S*)-1, 2,3, 4-tetrahydro-2- phenyl-1-naphthalenamine hydrochloride (52 mg, 0.20 mmol) (prepared according to: E.
J. Pribyl et al, US patent No. 3388121 and J. W. Clader et al, Eur. Pat. Appl. No. EP 508425 Al, which are incorporated by reference herein for their disclosures in the preparation of (1R*, 2S*) -1,2, 3, 4-tetrahydro-2-phenyl-1-naphthalenamine hydrochloride) in 1, 2-dichloroethane (3 mL) and saturated aqueous NaHC03 solution (3 mL) was added thiophosgene (0.10 mL) at room temperature. The reaction mixture was stirred at room temperature for 1h, diluted with EtOAc (30 mL). The organic layer was separated and washed with saturated NaHCO3 (10 mL), brine (10 mL), and dried over Na2S04. Removal of solvent and excess thiophosgene in vacuo gave the title compound, which was used without purification in the next step.
8B: 5-chloro-6-[2-[[[(1R*,2S*)-1,2,3,4-tetrahydro-2-phenyl-1- naphthalenvllaminolthioxomethyl] hydrazinol- 3-pridinecarboxylic acid.
5-chloro-6-hydrazino-3-pyridinecarboxylic acid (37 mg, 0.20 mmol) was added to the solution of (IR*, 2S*)-1, 2,3, 4-tetrahydro-l-isothiocyanato-2-phenyl-naphthalene in
DMA (4 mL), and the reaction mixture was stirred at room temperature overnight. The resulting solution was used in the next step without further purification.
8C : 5-chloro-N-(tetrahydro-2-oxo-3-thienyl)-6-[2-[[[(1R*,2S*)-1, 2,3,4-tetraydro-2- phenyl-1-naphthalenvllaminolthioxomethvllhvdrazinol-3-pyridi necarboxamide.
To 5-chloro-6-[2-[[[(1R*,2S*)-1, 2,3, 4-tetrahydro-2-phenyl-1- naphthalenyl] amino]-thioxomethyl] hydrazino]-3-pyridinecarboxylic acid (0.20 mmol) in DMA (4mL) was added homocysteine thiolactone hydrochloride (38 mg, 0.25 mmol), DIPEA (0.15 mL), followed by HATU (95 mg, 0.25 mmol). The reaction mixture was stirred at room temperature for 2 h, and concentrated in vacuo. Added H20 (5 mL) to the residue and the solid was collected and recrystallized from EtOH to give the title compound (50 mg, 45% in 3 steps). IHNMR (DMSO-d6) : 8 9.29 (s, 1H), 8.87 (s, 1H), 8. 67 (d, J = 7.2 Hz, 1H), 8.43 (s, 1H), 8.00 (s, 1H), 7.39 (brs, 1H), 7.25-6. 75 (m, 9H), 6.00 (m, 1H), 4.79 (m, 1H), 3.45-3. 36 (m, 1H), 3.34-3. 18 (m, 3H, overlap with H20 in DMSO), 2.80-2. 64 (m, 1H), 2.30-2. 15 (m, 1H), 2.08-1. 88 (m, 2H) ppm. MS (ESI) (M+1) + : 552.
Example 9: 5-chloro-6-[2-[[[(4-chlorophenyl)phenylmethyl]amino] thioxomethyl] - hydrazino]-N- (tetrahydro-2-oxo-3-thienyl)-3-pyridinecarboxamide. 9A: 5-chloro-6-[2-[[[(4-chlorophenyl)phenylmethyl]amino]-thioxom ethyl]hydrazino]- 3- pyridinecarboxylic acid.
Following the general procedure of Example 8B : 5-chloro-6-hydrazino-3- pyridinecarboxylic acid (37 mg, 0.20 mmol) was added to the solution of 1-chloro-4-
(isothiocyanatophenylmethyl) benzene (0.20 mmol) [prepared from 4-chloro-a-phenyl- benzenemethanamine HC1 salt and thiophosgene in dichloroethane and saturated NaHC03 following general procedure Example 8A] in DMA (4 mL), and the mixture was stirred at room temperature overnight. The pyridinecarboxylic acid solution was used in the next step without further purification.
9B: 5-chloro-6-[2-[[[(4-chlorophenyl)phenylmethyl]amino]thioxome thyl]hydrazino]-N- (tetrahydro-2-oxo-3-thienyl)-3-pvridinecarboxamide.
To the above prepared 5-chloro-6-[2-[[[(4-chlorophenyl)phenylmethyl] amino] - thioxomethyl] hydrazino]-3-pyridinecarboxylic acid (0.20 mmol) in DMA (4mL) from Example 9A was added homocysteine thiolactone hydrochloride (38 mg, 0. 25 mmol), DIPEA (0.15 mL), followed by HATU (95 mg, 0.25 mmol). The reaction mixture was stirred at room temperature for 2 h, and concentrated in vacuo. H20 (5 mL) was added to the residue and the solid was collected and recrystallized from EtOH to give the title compound (51 mg, 47% in two steps).'HNMR (DMSO-d6): 8 9.54 (brs, 1H), 9.09 (brs, 1H),. 85-8. 72 (m, 1H), 8.68 (d, J = 8 Hz, 1H), 8.55 (s, 1H), 8.05 (s, 1H), 7.40-7. 05 (m, 9H), 6.95 (d, J = 8.8 Hz, 1H), 4.85-4. 75 (m, 1H), 3. 46-3. 39 (m, 1H), 3.35-3. 20 (m, 1H, overlap with H20 in DMSO) 2.50-2. 40 (m, 1H, overlap with DMSO), 2. 30-2. 16 (m, 1H) ppm. MS (ESI) (M+1) + : 546.
Example 10: 5-chloro-6- [2- [ [ (1, 2-diphenylethyl) amino] thioxomethyl]-hydrazinol-N- (tetrahydro-2-oxo-3-thienyl)-3-pyridinecarboxamide.
10A : 5-chloro-6-[2-[[(1,2-diphenylethyl)amino]thioxomethyl]hydraz ino]- 3- pvridinecarboxylic acid.
Following the general procedure of Example 8B: The solution of 5-chloro-6- hydrazino-3-pyridinecarboxylic acid (37 mg, 0.20 mmol) and 1,2-diphenylethylisothio- cyanate (0.20 mmol) [prepared from 1,2-diphenylethylamine and thiophosgene in dichloroethane and saturated NaHC03 following general procedure Example 8A] in DMA (4 mL) was stirred at room temperature overnight. The pyridinecarboxylic acid solution was used in the next step without further purification.
10B : 5-chloro-6-[2-[[(1,2-diphenylethyl)amino]thioxomethyl]hydraz ino]-N-(tetrahydro- 2-oxo-3-thienyl)- 3-pyridinecarboxamide.
To the above prepared 5-chloro-6-[2-[[(1, 2-diphenylethyl) amino] iioxomethyl]- hydrazino] -3-pyridinecarboxylic acid (0.20 mmol) in DMA (4mL) from Step 10A was added homocysteine thiolactone hydrochloride (38 mg, 0. 25 mmol), DIPEA (0.15 mL), followed by HATU (95 mg, 0.25 mmol). The reaction mixture was stirred at room temperature for 2 h, and concentrated in vacuo. H20 (5 mL) was added to the residue and the solid was collected and recrystallized from EtOH to give the title compound (36 mg, 34% in two steps).'HNMR (DMSO-d6): 8 9.32 (d, J = 3.2 Hz, 1H), 9.09 (brs, 1H), 8.72 (d, J = 8.0 Hz, 1H), 8.48 (s, 1H), 8.19 (brs, 1H), 8. 11 (s, 1H), 7.35-7. 05 (m, 10H), 5.76- 5.66 (m, 1H), 4.88-4. 78 (m, 1H), 3.50-3. 40 (m, 1H), 3. 36-3. 26 (m, 1H, overlap with H20 in DMSO), 3.13 (dd, J = 9.0, 14. 0 Hz, 1H), 2.96 (dd, J = 6.0, 14.0 Hz, 1H), 2.52-2. 42
(m, 1H, overlap with DMSO), 2.34-2. 20 (m, 1H) ppm. MS (ESI) (M+1) + : 526. Anal.
Calcd for C2sH24ClNsO2S2. 0.25H2O : C, 56.59 ; H, 4.65 ; N, 13.20. Found : C, 56. 52; H, 4.33 ; N, 13.23.
Example 11: N-[3-Chloro-4-[[[[(10,11-dihydro-5H-dibenzo [a, d] cyclohepten-5- yl) aminolcarbonyl] amino] methyl] benzoyl]-N-methyl-glycine, ethyl ester.
1 lA : 4- (Bromomethvl)-3-chlorobenzonitrile CI CON Cl CN NBs/benzoyl peroxide vCN / /CCI4, reflux Br To a stirred suspension of 3-chloro-4-methylbenzonitrile (4.55 g, 30 mmol) and NBS (5.52 g, 31 mmol) in CCL (40 mL) was added benzoyl peroxide (110 mg). The reaction mixture was heated to reflux for 3 h, allowed to cool to room temperature and filtered through Celite. The Celite pad was washed with 10 mL of CCL4, and the filtrate was concentrated in vacuo. The product was purified by recrystallization from ethanol and hexanes to yield the title compound as white crystals (3. 46 g, 50%). H NMR (CDC13) : 8 7.69 (d, J=1. 5Hz, 1H), 7. 62-7. 54 (m, 2H), 4.58 (s, 2H) ppm.
11B : 1,1-dimethylethyl-[(2-chloro-4-cyanophenyl)methyl]-[(1,1dime thylethoxy]- carbamate.
To a stirred solution of 4-(bromomethyl)-3-chlorbenzonitrile (1.1 g, 4. 76 mmol) in DMF (15 mL) was added HN (Boc) 2 (1. 14 g, 5.24 mmol) followed by Cs2CO3 (1.71 g,
5.24 mmol). The resulting mixture was stirred at room temperature overnight. The light brown reaction mixture was then concentrated in vacuo,. and the residue was taken up into EtOAc (50 mL). The organic phase was washed with water (2 x 10 mL), brine (1 x 10 mL), and then concentrated in vacuo to provide the title compound as a light brown oil (1.67 g, 96%). This material was used in the step without further purification.'H-NMR (CDC13) : 8 7.49 (s, 1H), 7.41 (d, J=6.3 Hz, 1H), 7.14 (d, J=6.3 Hz, 1H), 4.77 (s, 2H), 1.30 (s, 18H) ppm.
11 C : 4-(AminomethY11-3-chlorobenzonitrile.
To a solution of 1, 1-dimethylethyl-[(2-chloro-4-cyanophenyl) methyl] [ (l, l- dimethylethoxy] carbamate (1.67 g, 4.55 mmol) in CH2C12 (5 mL) was added TFA (2.8 mL, 36.4 mmol). The resulting mixture was stirred at room temperature overnight. The reaction mixture was then concentrated in vacuo, and the residue was taken up into DCM (80 mL). The organic phase was washed with saturated NaHCO3 (2 x 20 mL), brine (1 x 20 mL), dried over Na2S04, filtered, and concentrated in vacuo to provide the title compound as a light brown oil (0.76 g, quantitative). This material was used in the following step without further purification.'H-NMR (CD30D): 8 7.89 (s, 1H), 7.76-7. 70 (m, 2H), 5.29 (s, 2H) ppm.
1 nid : 4- (Aminomethyl)-3-chloro benzoic acid hydrochloride. 0 ci CN ci C conc HCI XOH sealed tube NH2 heat NH2 CIH 2 2 4- (Aminomethyl)-3-chlorobenzonitrile (0.76 g, 4.55 mmol) was suspended in cone. HCl (3 mL) and the mixture was stirred at 150°C in a sealed tube for 8 h, allowed to cool to room temperature. The solid was collected and washed with Et20 to afford the title compound (0.87 g, 87%). This material was used in the step without further purification. MS (ESI) (M+H) + 6.
1 IE : 3-Chloro-4-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5-y l)amino]carbonyl- laminolmethvll-benzoic acid. 0 ci NCO ''"YTOH ci C OH N\/NH pyridine/DMA ci- N"z
Pyridine (1.21 mL, 15 mmol) was added to a solution of 4-(Aminomethyl)-3- chloro benzoic acid hydrochloride (666 mg, 3.0 mmol) in DMA (25 mL). The mixture was stirred at room temperature for 30 min, and then 10, 11-dihydro-5-isocyanato-5H- dibenzo [a, d] cycloheptene (777 mg, 3.3 mol) was added and the mixture was stirred at room temperature overnight. The reaction mixture was concentrated in vacuo, and water (10 mL) was added. The precipitate was isolated and washed with ethyl acetate, diethyl ether, and dried to give the title compound (1.02 g, 82%). This material was used in the next step without further purification. MS (ESI) (M+H) + = 421.
11F : N-[3-Chloro-4-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten- 5-yl)amino]- carbonlamino] methyl] benzoyll-N-methyl-glycine ethyl ester.
To a stirred solution of 3-Chloro-4-[[[[(10, 11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl] amino] methyl] -benzoic acid (60 mg, 0.143 mmol) in DMA (2 mL) was added HATU (65 mg, 0.172 mmol) followed by DIPEA (34 µL, 0.172 mmol). The mixture was stirred at room temperature for 10 min, and then sarcosine ethyl ester hydrochloride (26.4 mg, 0. 172 mmol) was added. The resulting mixture was stirred at room temperature for 4 h, and concentrated in vacuo. The residue was taken up in CH2Cl2 (20 mL), and washed with saturated NaHC03 (2 x 10 mL) and
then brine (1 x 10 mL). The material obtained after removal of the solvent was purified by reverse-phase HPLC (20-70% MeCN in water). Isolated fractions containing the pure product were treated with NaHC03 (excess) powder. The supernatants were all combined, concentrated in vacuo and extracted with ethyl acetate (2 x 20 mL). The ethyl acetate extract was washed with brine, dried over Na2S04 and concentrated to provide the title compound (23. 8 mg, 32%). 1H-NMR (CD3OD) : 8 7.45-7. 35 (m, 1H), 7.34-7. 26 (m, 4H), 7.14-7. 05 (m, 6H), 6.20 (s, 1H), 4.84 (s, 2H), 4.40 (s, one rotamer, 1. 5H), 4.38 (s, one rotamer, 0. 5H), 4.22-4. 10 (m, 2H), 3.28-3. 08 (m, 4H), 3.03 (s, one rotamer, 1H), 2.96 (s, one rotamer, 2H), 1.26 (t, one rotamer, J=7.6 Hz, 2H), 1.17 (t, one rotamer, J=7.6 Hz, 1H) ppm. MS (ESI) (M+H) + = 520.
Example 12: 3-Chloro-4- [ [ [(10, 11-dihydro-5H-dib enzo [a, d] cyclohepten-5- yl) amino] carbonyl] amino] methyl]-N-[[(2R)-1-ethyl-2-pyrrolidinyl] methyl]- benzamide.
Following general procedure 11F : To a stirred solution of 3-Chloro-4-[[[[(10,11- dihydro-5H-dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl] amino] methyl] -benzoic acid (60 mg, 0.143 mmol) in DMA (2 mL) was added HATU (65 mg, 0. 172 mmol) followed by DIPEA (34 iL, 0.172 mmol). The mixture was stirred at room temperature for 10 min, then (S)- (-)-2-aminomethyl-1-ethylpyrrolidine (22.0 mg, 0.172 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. After work-up, the crude product was purified by reverse-phase HPLC (20-70% MeCN in water) affording the title compound (29.6 mg, 37%).'H-NMR (CD30D): 8 7.74 (s, 1H), 7.49 (d, J = 6. 0 Hz, 1H), 7.37 (d, J = 6.0 Hz, 2H), 7.29-7. 24 (m, 3H), 7.22-7. 11 (m, 4H), 5.93 (d, J = 4. 2 Hz, 1H), 5.41 (s, br, 1H), 5.11 (s, br, 1H), 4.41 (d, J = 4.8 Hz, 2H), 3.66-3. 59 (m, 1H), 3.45-3. 26 (m, 4H), 3.11-2. 99 (m, 2H), 2.95-2. 76 (m, 2H), 2.43-2. 25 (m, 2H), 1.84-1. 70 (m, 2H), 1.16 (t, J = 5.4 Hz, 3H) ppm. MS (ESI) (M+H)'*'=531.
Example 13 : 3-Chloro-4-[[[[(10,11-diydro-5H-dibenzo[a,d]cyclohepten-5-yl )- amino] carbonyl] amino] methyl]-N- [2- (l-methyl-2-pyrrolidinyl) ethyl]-benzamide.
Following general procedure Example 11F : To a stirred solution of 3-Chloro-4- [[[[(10,11-dihydro-5H-dibenzo[a, d] cyclohepten-5-yl) amino] carbonyl] amino] methyl]- benzoic acid (60 mg, 0.143 mmol) in DMA (2 mL) was added HATU (65 mg, 0.172 mmol) followed by DIPEA (34 1L, 0.172 mmol). The mixture was stirred at room temperature for 10 min, then 2-(2-aminoethyl)-1-methylpyrrolidine (22.0 mg, 0.172 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. After work-up, the crude product was purified by reverse-phase HPLC (20-70% MeCN in water) to provide the title compound (16.4 mg, 21c/o). lH-NMR (CD30D) : 8 9.00 (d, J= 4. 2 Hz, 1H), 7. 50 (d, J= 1. 5 Hz, 1H), 7. 38 (d, J=5. 7Hz, 2H), 7. 26 (m, 1H), 7. 14-7.02 (m, 6H), 6.64 (d, J= 6. 0 Hz, 1H), 6.24 (d, J= 6.0 Hz, 1H), 6.06 (t, J= 4.8 Hz, 1H), 4.31 (d, J= 4.8 Hz, 2H), 3.58-3. 48 (m, 1H), 3. 33-3. 18 (m, 2H), 3.15-3. 03 (m, 2H), 2.44-2. 37 (m, 1H), 2.32 (s, 3H), 2.23-2. 15 (m, 1H), 1.98 (s, br, 1H), 1.91-1. 78 (m, 1H), 1.77-1. 58 (m, 4H) ppm. MS (ESI) (M+H) + = 531.
Example 14: 3-Chloro-4-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) aminolcarbonyl] amino] methyl]-N- [3-(2-methyl-1-piperidinyl)propyl]- benzamide.
Following general procedure Example 1 IF : To a stirred solution of 3-Chloro-4- [ [ [[(10,11-dihydro-5H-dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl] amino] methyl] - benzoic acid (60 mg, 0.143 mmol) in DMA (2 mL) was added HATU (65 mg, 0.172 mmol) followed by DIPEA (34 yL, 0.172 mmol). The mixture was stirred at room
temperature for 10 min, and then 1- (3-aminopropyl)-2-pipecoline (26.9 mg, 0.172 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. After work-up, the crude product was purified by reverse-phase HPLC (20-70% MeCN in water) to provide the title compound (24. 8 mg, 31%).'H-NMR (CD30D): 5 9.14 (s, 1H), 7. 57 (d, J= 1.2 Hz, 1H), 7.42-7. 34 (m, 3H), 7.15-7. 01 (m, 6H), 6.91 (d, J= 6.0 Hz, 1H), 6.29 (m, 2H), 4. 34 (d, J= 4.5 Hz, 2H), 3.54-3. 43 (m, 1H), 3.26-3. 06 (m, 4H), 2.94-2. 84 (m, 2H), 2.31-2. 17 (m, 2H), 2.04-1. 94 (m, 1H), 1.79-1. 51 (m, 4H), 1.49-1. 38 (m, 1H), 1. 36-1. 21 (m, 2H), 1.02 (d, J= 4. 8 Hz, 3H) ppm. MS (ESI) (M+H) += 559.
Example 15: N-[[5-chloro-6-[ [ [l (10, 11-dihydro-SH-dibenzo la, d] cyclohepten-5- yl) amino] carbonyl] amino] methyl]-3-pyridinyl] carbonyll-N-methyl-glycine ethyl ester.
15A : Methyl 5, 6-dichloro-3-pyridinecarboxylate.
To a stirred solution of 5, 6-dichloro-3-pyridinecarboxylic acid (1.92 g, 10 mmol) in MeOH (30 mL) cooled to 0°C, thionyl chloride (0.726 mL, 10 mmol) was added dropwise and the resulting mixture was stirred at room temperature overnight. The reaction mixture was then concentrated in vacuo, and the residue was taken up in CH2Cl2 (50 mL). The organic phase was washed with saturated NaHC03 (2 x 10 mL) and brine (1 x 10 mL), dried over MgS04, filtered, and concentrated in vacuo to provide the title compound as a white solid (1.39 g, 67%). This material was used in the next step without further purification.'H-NMR (CDC13) : 6 8. 89 (s, 1H), 8.37 (s, 1H), 3.98 (s, 3H) ppm.
15B: Methyl 5-chloro-6-cvano-3-pvridinecarboxvlate. 0 0 Cl A) t NaCN/DMAP (cat.) ci 0 Y EtCN, reflux CI N NC N
To a stirred solution of methyl 5,6-dichloro-3-pyridinecarboxylate (824 mg, 4.0 mmol) in EtCN (40 mL) was added NaCN (294 mg, 6. 0 mmol) and then DMAP (80 mg, 10% w. t. ). The resulting mixture was stirred at 99°C overnight and cooled to room temperature. The reaction mixture was then concentrated in vacuo, water (30 mL) was added and extracted with CH2C12 (3x 20 mL). The organic layers were combined, washed with water (2 x 10 mL), brine (1 x 10 mL), dried over MgS04, and concentrated in vacuo.
The product was purified by flash chromatography (10-15% EtOAc in Hexanes) to provide the title compound as a white solid (508 mg, 65%). 1H-NMR (CDCl3) : 8 9.17 (s, 1H), 8.46 (s, 1H), 4.03 (s, 3H) ppm. MS (ESI) (M+H)+ = 197.
15C : Methyl 6-(aminomethyl)-5-chloro-3-pandinecarboxylate. 0 0 Raney Ni/H2 Cl) t MeOH, r. t., 48h NC NJ NJ NH2 Raney nickel (slurry in water, cat. ) was added to a solution of methyl 5-chloro-6- cyano-3-pyridinecarboxylate (393 mg, 2.0 mmol) in MeOH (40 mL). The resulting mixture was hydrogenated at 40 psi until the reaction was complete by LC/MS (48 h).
After filtration, the green filtrate was concentrated in vacuo, and the residue was used directly for the next step without further purification. MS (ESI) (M+H) = 201.
15D : Methyl 5-chloro-6-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) aminolcarbonvllamino] methyll-3-pyridinecarboxylate. o ci UNCO cri 0 H _ N'NH NJ pyridine/DMA II MHz
Following general procedure Example HE : To a solution of the above crude methyl 6- (aminomethyl)-5-chloro-3-pyridinecarboxylate (from 2.0 mmol of precursor) in DMA (10 mL) was added pyridine (0.81 mL, 10 mmol). The mixture was stirred at room temperature for 30 min, and then 10, 11-dihydro-5-isocyanato-5H-dibenzo [a, d] cyclo- heptene (565 mg, 2.4 mol) was added and mixture was stirred at room temperature overnight. The reaction mixture was then concentrated in vacuo, and triturated with water (10 mL). The precipitate was collected by filtration, washed with methanol, and then dried to give the title compound as a white solid (620 mg, 72%). This material was used for the next step without further purification. MS (ESI) (M+H) + = 436.
15F: 5-chloro-6-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5-y l)amino]carbonyl]- aminolmethyll-3-pyridinecarboxvlic acid. 0 0 cl - H ! NaOH //f""N 1 0 OH I j N NU MeOHlTHF/H20 N'NH < reflux, 30 min II O zu To a stirred suspension of methyl 5-chloro-6-[[[[(10,11-dihydro-5H-dibenzo [a, d]- cyclohepten-5-yl) amino] carbonyl] amino] methyl]-3-pyridinecarboxylate (620 mg, 1.43 mmol) in MeOH/THF/H20 (1 : 1 : 1,45 mL) was addedNaOH (284 mg, 7. 1 mmol). The resulting mixture was refluxed for 30 min during which time the solution became clear.
After cooling of the reaction mixture to room temperature, the organic solvents were removed in vacuo, and the aqueous slurry was neutralized with 10% acetic acid to pH = 6-7. The precipitate was collected by filtration, washed with water and dried to give the title compound as a white solid (580 mg, 97%). This material was used in the next step without further purification. MS (ESI) (M+H) + = 422.
15G : N-[[5-Chloro-6-[[[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten -5-yl)amino]- carbonvl] amino] methyll-3-pyridinlcarbonyl]-N-methyl-glycine ethyl ester.
Following general procedure Example 11F : To a stirred solution of 5-chloro-6- [ [ [ [ (10, ll-dihydro-5H-dibenzo [a, d] cyclohepten-5-yl) amino] carbonyl] amino] methyl]-3- pyridinecarboxylic acid (84 mg, 0.20 mmol) in DMA (3 mL) was added HATU (91 mg, 0.24 mmol) followed by DIPEA (47 µL, 0.24 mmol). The mixture was stirred at room temperature for 10 min, and then sarcosine ethyl ester hydrochloride (36. 8 mg, 0.24 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. After work-up, the crude product was purified by preparative TLC (5% Hexanes in EtOAc) to provide the title compound (8.8 mg, 8. 5%). 1H-NMR (CDCI3) : 8 8.40 (s, 0.75H, one rotamer), 8.34 (s, 0.25H, one rotamer), 7.74 (s, 0.75H, one rotamer), 7.66 (s, 0.25H, one rotamer), 7.40 (d, J= 6. 0 Hz, 2H), 7.18-7. 07 (m, 6H), 5.97 (s, br, 1H), 5.90 (s, br, 1H), 5.58 (s, br, 1H), 4.60-4. 53 (m, 2H), 4.25-4. 16 (m, 4H), 3.48-3. 37 (m, 2H), 3.10-3. 04 (m, 2H), 3.03 (s, one rotamer, 1H), 3.01 (s, one rotamer, 2H), 1.30-1. 24 (m, 3H) ppm. MS (ESI) (M+H) += 521.
Example 16: N-[[5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepte n-5- yl) amino] thioxomethyl] hydrazino]-3-pyridinyl] sulfonyl]-N-methyl-glycine, ethyl ester.
16A: N-[(5,6-dichloro-3-pyridinyl0sulfonyl]-N-methyl- glycine, ethyl ester.
A solution of 5, 6-dichloro-3-pyridinesulfonyl chloride (Prepared according to: M. W. Crawley, European Patent Application EP0147105A2 (1985), which is incorporated by reference herein for its disclosure in the preparation of 5, 6-dichloro-3-pyridinesulfonyl chloride) (246 mg, 1.00 mmol) in CH2C12 (5 mL) was added to a mixture of sarcosine ethyl ester hydrochloride (191 mg, 1.25 mmol) and DIPEA (0.60 mL, 3.5 mmol) in CH2CI2 at room temperature. The reaction mixture was stirred at room temperature for 3 h, diluted with EtOAc (50 mL), washed with 5% HC1, H20, and brine, dried over Na2S04. Removal of solvent gave the title compound (302 mg, 92%), MS (ESI) (M+1) + : 327.
16B: N-[(5-chloro-6-hydrazino-3-pyridinyl)sulfonyl]-N-methyl- glycine, ethyl ester.
1 M hydrazine (2 mL, 2 mmol) in EtOH was added to the solution of N- [ (5, 6- dichloro-3-pyridinyl) sulfonyl]-N-methyl-glycine ethyl ester (302 mg) in EtOH (5 mL), and the mixture was heated to reflux for 3 h, allowed to cool to room temperature, and concentrated in vacuo. The residue was triturated with H20 (3 mL), and the light yellow solid (230 mg, 77%) was collected and dried. MS (ESI) (M+l) = 323.
16C: N-[[5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepte n-5- yl) aminolthioxomethvllhvdrazinol-3-pyridinvllsulfonyl-N-methyl- glycine, ethyl ester.
The mixture of the hydrazine compound from 16B (52 mg, 0.16 mmol) and 10,11- dihydro-5-isothiocyanato-5H-dibenzo [a, d] cycloheptene (50 mg, 0.20 mmol), pyridine (50 , ut) in DMA (2 mL) was stirred at room temperature overnight. The solvent was
evaporated and the residue was triturated with Et20 (5 mL). The solid was collected and recrystallized in MeOH to the title compound (65 mg, 71%). IHNMR (DMSO-d6): 8 9.74 (9.60) (s, 1H), 9.02 (br, 1H), 8.45 (s, 1H), 8.08 (s, 1H), 7. 55 (br, 1H), 7.40-7. 05 (m, 8H), 7.03 (brs, 1H), 4.05 (s, 2H), 4.05 (q, J = 6. 8 Hz, 2H), 3.40-3. 10 (m, 2H, overlap with H20 in DMSO), 3.00-2. 85 (m, 2H), 2.79 (s, 3H), 1.14 (t, J = 6.8 Hz, 3H) ppm. MS (ESI) (M+1) + : 574.
Example 17 : N-[[5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepte n-5- yl) amino] carbonyl] hydrazino]-3-pyridinyl] sulfonyl]-N-methyl-glycine, ethyl ester.
The mixture of N- [ (5-chloro-6-hydrazino-3-pyridinyl) sulfonyl]-N-methyl- glycine, ethyl ester from Example 16B (32 mg, 0.10 mmol) and 10, 11-dihydro-5- isocyanato-SH-dibenzo [a, d] cycloheptene (28 mg, 0.12 mmol), pyridine (50 µL) in DMA (2 mL) was stirred at room temperature overnight. The solvent was evaporated and the residue was triturated with Et20 (5 mL). The solid was collected and recrystallized in MeOH twice to the title compound (25 mg, 45%). 1HNMR (DMSO-d6): 8 9.19 (s, 1H), 8.32 (d, J = 2 Hz, 1H), 7. 99 (s, 1H), 7.61 (d, J = 4Hz, 1H), 7.59 (d, J = 4 Hz, 1H), 7.33 (d, J = 7.6 Hz, 2H), 7.20-7. 05 (m, 6H), 6. 26 (br, 1H), 4.03 (s, 2H), 3.98 (q, J = 7. 2 Hz, 2H), 3.25-3. 05 (m, 4H), 2.76 (s, 3H), 1. 12 (t, J = 7.2 Hz, 3H) ppm. MS (ESI) (M+I) + : 558.
Example 18: 5-Chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) aminol thioxomethyl] hydrazino] -N- [ (3R)-l-methyI-2-oxo-3-pyrroIidinyI]-3- pyridinecarboxamide. 18A : fflR)-l-r (methvlamino) carbonyll-3- (methylthio) propyll-carbamic acid, l, l- dimethylethyl ester.
HATU (9.203 g, 24. 22 mmol) was added in one portion to a solution of Boc-D- methionine (5.023 g, 20.15 mmol), MeNH2. HCl (2.035 g, 30. 14 mmol), and DIPEA (11 ml, 63.8 mmol) in DMF (100 ml) at 0°C. The mixture was then stirred at room temperature for 2 h, and concentrated in vacuo to remove DMF and excess DIPEA, and H2O (100 ml) was added. The mixture was then extracted with CH2C12 (2x150 ml), the combined extracts were washed with saturated NaHC03 (30 ml) and brine (30 ml), dried over Na2S04. Removal of solvent produced product which was purified by flash chromatography on silica gel pretreated with 1% Et3N in CH2C12 (eluent MeOH : CH2Cl2 = 1 : 30) to give the title compound (3.40 g, 63%).
18B: [ 3R)-1-methyl-2-oxo-3-pyrrolidinyll-carbamic acid, l, l-dimethylethyl ester.
[(1R)-1-[(methylamino)carbonyl]-3-(methylthio) propyl] -carbamic acid, 1,1- dimethylethyl ester from step 18A (1. 521 g, 5,80 mmol) was dissolved MeI (20 ml), and
the mixture was stirred at room temperature overnight. Excess MeI was removed in vacuo, and the residue was kept under vacuum pump for 2 h to give the intermediate [(3R)-3-[[(1,1-dimethylethoxy) carbonyl] amino]-4- (methylamino)-4-oxobutyl] dimethyl- sulfonium, iodide salt (2.65 g, quantitatively). The sulfonium salt (816 mg, 2.02 mmol) was dissolved in anhydrous THF (40 ml), and the solution was cooled to 0°C in ice-bath.
LiHMDS (1M, 2.05 ml, 2.05 mmol) was added dropwise to the above cold solution. The reaction mixture was stirred at room temperature for 2 h, and Hz0 (5 ml) was added. The majority of THF was evaporated, and the residue was dissolved CH2Cl2 (100 ml) and washed with H20 (20 ml), the aqueous phase were extracted with CH2Cl2 (50 ml). The combined CH2CI2 solution were dried and concentrated in vacuo. The residue was purified on silica gel pretreated with 1% Et3N in CH2Clz (eluent MeOH : CH2C12 1: 25) to afford a light yellow solid. The light yellow solid was washed with Et20 to produce a white solid (220 mg).'HNMR (CDCl3) : 8 5.12 (br, 1H), 4.14 (m, 1H), 3.28-3. 38 (m, 2H), 2. 89 (s, 3H), 2.60-2. 70 (m, 1H), 1.82-1. 93 (m, 1H), 1.45 (s, 9H) ppm.
18C: (3R)-amino-1-methyl-2-pyrrolidinone, monohydrochloride.
[ (3R)-l-methyl-2-oxo-3-pyrrolidinyl]-carbamic acid, 1,1-dimethylethyl ester (220 mg, 1.02 mmol) from Example 18B was dissolved in EtOAc (20 ml), and HCl (g) was bubbled into the solution for 10 min, the mixture was then stirred at room temperature for 2 h, and the solvent was evaporated to give the compound (3R)-amino-1-methyl-2- pyrrolidinone, HCI salt (103 mg).
18D: 5-chloro-6-[2-[[(10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-pyrro lidinyl]- 3- pyridinecarboxamide,.
Following the general HATU coupling procedure of Example 1C : the title compound (110 mg, 23%) was prepared from 5-chloro-6-[2-[[(10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] thioxo-methyl] hydrazino]-3-pyridinecarboxylic acid. DMA (460 mg) and (3R)-amino-l-methyl-2-pyrrolidinone, HC1 salt (180 mg).
1HNMR (DMSO-d6): 6 9.67 (s, 1H), 9.25 (brs, 1H), 8.89 (brs, 1H), 8. 69 (d, J = 6. 8 Hz, 1H), 8.57 (s, 1H), 8.13 (s, 1H), 7.51 (br, 1H), 7.29 (d, J = 6. 4 Hz, 2H), 7.20-6. 90 (m, 6H), 4.45-4. 60 (m, 1H), 3.10-3. 40 (m, 4H), 2.96 (m, 2H), 2.75 (s, 3H), 2.31 (br, 1H), 1.90 (m, 1H) ppm. MS (ESI) (M+1)+ = 535. 17. Anal. Calcd for C27H27ClN6O2S.H2O : C, 58.63 ; H, 5.28 ; N, 15.15. Found: C, 58.76 ; H, 5.15 ; N, 14.83.
Example 19 : 5-chloro-6- [2- [ [ (3-chloro-10, 11-dihydro-5H-dibenzo [a, d]cyclohepten-5- yl) amino] thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-pyrrolidiny l]-3- pyridinecarboxamide.
19A: 2-f2- (4-chlorophenyl) ethyll- benzoic acid.
The mixture of Phthalic anhydride (10.80 g, 72.97 mmol) and 4-Cl-phenylacetic acid (9. 40 g, 55.10 mmol), NaOAc (0.56 g, 6.83 mmol) was heated at 210-230°C for 2 h, allowed to cool to 80-90°C, EtOH (50 ml) was added, the solid was collected and washed with EtOH (30 ml), followed by heane/EtOH (9 : 1, 20 ml), dried to give the intermediate (11. 50 g).
The above intermediate (11.50 g) was added to 57% HI (60 ml) and red phosphorus (4.50 g) was added, the mixture was then heated at 160-165 °C for 16 h, allowed to cool to room temperature, poured into crushed ice (400 g), then neutralized with 50% KOH to pH-14. The solid was filtered off, the filtrate was acidified with 12 N HCl to pH#1, the white solid was collected and dried to give the title compound (6.50 g).
MS (ESI) (M+1+CH3CN)+=302.
19B: 3-chloro-10, 11-dihydro-5H-dibenzoSa, dlcyclohepten-5-one.
Thionyl chloride (10 ml) was added to a solution of 2- [2- (4-chlorophenyl) ethyl] - benzoic acid (3.50 g) in CH2Cl2 (100 ml). The mixture was stirred at reflux overnight, and the solvent and excess SOC12 was evaporated in vacuo. The residue was re-dissolved in CH2CI2 (100 ml) and AlCl3 (4. 50 g) was added, the reaction mixture was stirred at room temperature for 3 h, ice water (30 ml) was added. After stirring for another 20 min, the organic phase was separated and washed with H20 (20 ml), IN NaOH (2x20ml), brine (20 ml), dried over Na2SO4. The solvent was removed in vacuo and the residue was chromatographed on silica gel (EtOAc: CH2C12 1: 4) to afford the title compound (2.65 g).
19C: 3-chloro-10, dihvdro-5H-dibenzo [asdlcvclohepten-5-ol.
NaBH4 (86 mg, 2.26 mmol) was added portionwise to a solution of 3-chloro- 10, 11-dihydro-5H-dibenzo [a, d] cyclohepten-5-one (590 mg, 2.43 mmol) in MeOH (20 ml)
at room temperature overnight. MeOH was evaporated and H20 (20 ml) was added, the solid was collected and washed with H2O (20 ml), dried to give the title compound (590 mg).
19D: 3-chloro-10, 11-dihydro-SH-dibenzora, dlcyclohepten-5-amine.
SOC12 (1 ml) was added to a solution of 3-chloro-10, 11-dihydro-SH- dibenzo [a, d] cyclohepten-5-ol (590 mg, 2.42 mmol) in CH2Cl2 (10 ml), and the mixture was heated at reflux for 1 h, and CH2CI2 and excess SOC12 was evaporated in vacuo. The residue was re-dissolved in CHC13, and NH3 (g) was bubbled into the solution for 20 min, and the reaction mixture was stirred at room temperature for 2 h. (CHCI3 was evaporated in vacuo and the residue was dissolved in IN HCl (5ml) and washed with Et20 (20 ml).
Then the aqueous solution was neutralized with 1 N NaOH to pH-9-10, extracted with Et20 (50 ml), and the Et2O solution was washed brine (10 ml), dried over Na2SO4 and concentrated in vacuo to produce the title compound (281 mg, 48%).
19E: 3-chloro-10,11-dihydro-5-isothiocyanato-5H-dibenzo[a,d]cyclo heptene.
CSCl2 (0.5 ml) was added in one portion to a vigorously stirred solution of 3- chloro-10, 11-dihydro-5H-dibenzo [a, d] cyclohepten-5-amine (281 mg) in CH2Cl2 (15 ml) and saturated NaHCO3 (5 ml) at room temperature, the mixture was stirred at room temperature for 2 h and diluted with CH2Cl2 (30 ml), then the organic phase was separated and washed with brine (10 ml), dried over Na2SO4, and concentrated in vacuo to give the title compound (246 mg, 75%). 19F : 5-chloro-6- [2-[[(3-chloro-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5- yl) aminolthioxomethvllhhydrazinol-3-pridinecarboxylic acid.
A mixture of S-chloro-6-hydrazino-3-pyridinecarboxylic acid (37 ml, 0.20 mmol) and 3-chloro-10, 11-dihydro-5-isothiocyanato-5H-dibenzo [a, d] cycloheptene (58 mg, 0.20 mmol) in DMA (3 ml) was stirred at room temperature overnight. The title compound (0.20 mmol) as its DMA solution was ready for use.
19G : 5-chloro-6-[2-[[(3-chloro-10,11-dihydro-5H-dibenzo[a,d]cyclo hepten-5- yl) aminolthioxomethvllhvdrazinol-N-r (3R)-1-methyl-2-oxo-3-pvrrolidinyll- 3- pvridinecarboxamide.
(3R)-amino-1-methyl-2-pyrrolidinone, monohydrochloride (33 mg, 0.22 mmol) from Example 18C, followed by DIPEA (0. 2 ml) and HATU (5 mg, 0.25 mmol) was added the solution of 5-chloro-6-[2-[[(3-chloro-10,11-dihydro-5H- dibenzo [a, d] cyclohepten-5-yl) amino] thioxomethyl] hydrazino]- 3-pyridinecarboxylic acid (0.20 mmol) in DMA (3 ml) from Example 19F. The reaction mixture was stirred at room temperature for 2 h and then H20 (10 ml) was added, the precipitate was collected and recrystallized from MeOH to produce the title compound (56 mg).'HNMR (DMSO-d6) : 8 9.74 (s, 1H), 9.31 (s, 1H), 9.18 (d, J = 9.2 Hz, 1H), 8.73 (d, J = 8. 2 Hz, 1H), 8.63 (s, 1H), 8.16 (s, 1H), 7.67 (d, J = 8.2 Hz, 1H), 7.25-7. 45 (m, 2H), 5. 95-7.25 (m, 5H), 4.50- 4.65 (m, 1H), 3.34 (br, 4H), 2.95-3. 00 (m, 2H), 2.76 (s, 3H), 2.24-2. 39 (m, 1H), 1. 83-1.89 (m, 1H) ppm. MS (ESI) (M+1) +-569. HRMS (M+1) + Calcd for C27H26C12N602S : 569.1293 ; Found: 569.1205.
Example 20 : 5-chloro-6-[2-[[(6, 11-dihydrodibenzotb, e] thiepin-11- yl) amino] thioXomethyl] hydrazino]-N-[(3R)-tetrahydro-2-oxo-3-thienyl]-3- pyridinecarboxamide.
20A: 6,11-dihydro- dibenzo[b,e]thiepin-11-amine. // f NH20H. HCI OH Zn X Pyridine X X pyridine, \ NH20H. HCl (1.20 g) was added to a solution of dibenzo [b, e] thiepin-11 (6H) -one (1.20 g) in pyridine (20 ml), and the mixture was heated at reflux for 3 days, allowed to cool to room temperature, and pyridine was evaporated, and the residue was dissolved in CH2CI2 (100 ml), and washed with 2N HCl (2x20 ml), saturated NaHC03 (20 ml), brine, dried over Na2S04. Evaporation of solvent gave the oxime intermediate (605 mg).
The oxime intermediate (520 mg) was dissolved in EtOH (20 ml), and 5N NH40H (20 ml), NH40Ac and Zn (1.10 g) were added. The reaction mixture was heated at reflux overnight, allowed to cool to room temperature, diluted with EtOH (100 ml), and then filtered through celite. The solvent was evaporated and the residue was dissolved in Et20 (200 ml), washed with 1 N NaOH (20 ml), brine, dried over Na2S04. The solvent was removed the in vacuo and the crude product was re-dissoved in Et20 (50 ml), and 1 N HCl in Et2O was added until no more precipitae was formed. The solid was collected to give the title compound (260 mg) as its HCl salt.
20B: 6, 11-dihydro-11-isothiocyanato- dibenzo[b,e]thiepin.
-Following the general procedure of Example 19E : CSC12 (0. 15 ml) was added in one portion to a vigorously stirred solution of 6,11-dihydro- dibenzo[b, e] thiepin-11- amine, HCl salt (86 mg) in CH2Cl2 (5 ml) and saturated NaHCO3 (5 ml) at room temperature, the mixture was stirred at room temperature for 2 h and diluted with CH2Cl2 (20 ml), then the organic phase was separated and washed with brine (10 ml), dried over Na2SO4, and concentrated in vacuo to give the title compound (91 mg).
20C: 5-chloro-6-[2-[[(6,11-dihydrodibenzo[b,e]thiepin-11- vl) amino thioxomethvllhzinol-3-vridinecarboxvlic acid.
A mixture of 5-chloro-6-hydrazino-3-pyridinecarboxylic acid (37 ml, 0.20 mmol) and 6, 11-dihydro-11-isothiocyanato-dibenzo [b, e] thiepin (53 mg, 0. 20 mmol) in DMA (3 ml) was stirred at room temperature overnight. The title compound (0.20 mmol) as its DMA solution was ready for use.
20D: 5-chloro-6-[2-[[(6,11-dihydrodibenzo[b,e]thiepin-11- yllaminolthioxomethyllhvdrazino]-N- [(3R)-tetrahydro-2-oxo-3-thienvll- 3- pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1C : the title . compound (30. mg) was prepared from 5-chloro-6- [2- [ [ (6, 11-dihydrodibenzo [b, e] thiepin- 11-yl) amino] thioxomethyl] hydrazino]-3-pyridinecarboxylic acid (0.1 mmol) and racemic homocysteine thiolactone hydrochloride (17 mg).'HNMR (DMSO-d6) : # 9.78 (s, 1H), 9.29 (s, 1H), 8.87 (m, 1H), 8.71 (m, 1H), 8.49 (s, 1H), 8.14 (s, 1H), 7.00-7. 60 (m, 9H), 4.75-4. 85 (m, 1H), 4.68 (d, J = 14.1 Hz, 1H), 4.02 (d, J = 14.1 Hz, 1H), 3.35-3. 50 (m, 1H), 3.25-3. 35 (m, 1H), 2.40-2. 50 (m, 1H),, 2.15-2. 30 (m, 1H) ppm. MS (M+1) +=556.
HRMS (M+1) + Calcd for C25H22CINsO2S3 : 556.0702. Found: 556.0795.
Example 21: 5-Chloro-6-[2-[[ (6, 11-dihydro-6-methyl-5, 5- dioxidodibenzo [c, fl [1, 2] thiazepin-11-yl) amino] thioxomethyl] hydrazino]-N-1 (3R)- tetrahydro-2-oxo-3-thienyl]-3-pyridinecarboxamide.
21A: 6, 11-Dihydro-6-methyl-5, 5-dioxide-dibenzo (c, f] l, 21thiazepin-1l-ol.
NaBH4 (0.5 g, 13.16 mmol) was added to a solution of 6-methyl- dibenzo [c, fl [1, 2] thiazepin-11 (6H) -one, 5,5-dioxide (1.5 g, 5.49 mmol) (prepared according to the method described in Abramovitch, R. A. , Azogu, C. I., McMaster, I. T. and Vanderpool, D. P., J. Org. Chem. , 1978,43, 1218, which is incorporated by reference herein for its disclosure in the preparation of 6-methyl-dibenzo [c, f] [1, 2] thiazepin-
11 (6H) -one, 5,5-dioxide) in MeOH/CH2C12 (50/15 ml) and the reaction was stirred for 2 hours. The solvents were then removed under reduced pressure and the residue was diluted in CH2CI2 and was washed with saturated NaHCO3, water, and brine, dried and concentrated under reduced pressure to provide alcohol which was used directly for the next step (1.5 g). 1H NMR (400 MHz, CDC13) : 8 7.98 (d, J = 7.6 Hz, 1H), 7.70-7. 58 (m, 3H), 7.55-7. 49 (m, 1H), 7.42-7. 25 (m, 3H), 5.91 (d, J = 10. 3 Hz, 1H), 4.42 (d, J = 10. 3 Hz, 1H), 3.13 (s, 3H) ppm. MS (ESI) (M+1) + : 276.
21B: 6,11-Dihydro-6-methyl-5,5-dioxide-dibenzo[c,f][1,2]thiazepin -11-amine.
Following the same procedure of Example 19D : the title compound (200 mg, 13% in two steps) was prepared from 6, 11-dihydro-6-methyl-5, 5-dioxide- dibenzo [c, f] [1, 2] thiazepin-l l-ol (1. 5 g, 5.49 mmol).'H NMR (400 MHz, CDC13) : 8 7.98 (d, J = 7. 8 Hz, 1H), 7. 62-7.51 (m, 2H), 7.49 (d, J = 7.4 Hz, 1H), 7.44 (t, J = 7.2 Hz, 1H), 7.38-7. 24 (m, 3H), 5.45 (s, 1H), 3.26 (s, 3H), 2. 23 (bs, 2H) ppm. MS (ESI) (M+1) + : 275.
21 C : 6, 11-Dihydro-11-isothiocyanato-6-methyl-55-dioxide-dibenzorcs 2lthiazepine.
Following the general procedure of Example 19E : To a virgorously stirred solution of 6, 11-dihydro-6-methyl-5, 5-dioxide-dibenzo [c, f] [1, 2] thiazepin-11-amine (0.16 g, 0. 58 mmol) in CH2C12 (8.7 ml) and saturated NaHCO3 solution (8.7 ml) was added thiophosgene (0.29 mL, 3.8 mmol) in one portion at room temperature. The mixture was stirred for 2 hours and then was diluted in EtOAc. Aqueous phase was then separated and the organic phase was washed several times with saturated NaHC03 and then with brine, dried and concentrated under reduced pressure. The product was directly used for the next step.
21D: 5-Chloro-6-[2-[[(6,11-dihydro-6-methyl-5,5-dioxidodibenzo[c, f][1,2]thiazepin-11- vI) aminolthioxomethyl] hydrazinol-3-pyridinecarboxylic acid.
5-Chloro-6-hydrazino-3-pyridinecarboxylic acid (115 mg, 0. 62 mmols, 1.1 eq) was added to a solution of 6, 11-dihydro-11-isothiocyanato-6-methyl-5, 5-dioxide- dibenzo [c, f] [1, 2] thiazepine (0.58 mmols, 1 eq. ) in DMA (3 mL). The reaction was stirred overnight and then concentrated under reduced pressure. The residue was diluted in CH2Cl2 and was washed with saturated NaHCO3, water and brine, dried and concentrated.
The product was then used directly for the next step. MS (ESI) (M+1) + : 504.
21E : 5-Chloro-6-[2-[[(6,11-dihydro-6-methyl-5,5-dioxidodibenzo[c, f][1,2]thiazepin-11- ylaminolthioxomethyl hvdrazinol-N-F (3R)-tetrahydro-2-oxo-3-thienyl-3- pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1C : HATU (27 mg, 0.7 mmol) was added to a mixture of D-homocysteine thiolactone. HCl (11 mg, 0.07 mmol), 5-chloro-6-[2-[[(6,11-dihydro-6-methyl-5,5-dioxidodibenzo[c, f][1, 2] thiazepin-11- yl) aminothioxomethylghydrazino]-3-pyridinecarboxylic acid (30 mg, 0.06 mmol), and DIPEA (25 FL, 0.14 mmol) in DMA (3ml). The mixture was stirred at room temperature for 2 hours and then DMA was removed under reduced pressure. The residue was diluted in CH2Cl2 and was washed successively with saturated NaHCO3, water and brine, dried and concentrated. The product was then purified by flash chromatography (CH2C12- MeOH) to provide the title compound (11 mg, 31%). 1H NMR (400 MHz, CD30D): 8 8.99 (bd, J = 7. 6 Hz, 2H), 8.23 (d, J = 1.95 Hz, 1H), 8.16 (d, J = 1.95 Hz, 1H), 8.00 (dd, J
= 7.62 and 2.93 Hz, 2H), 7.95 (dd, J = 5. 1 and 1.95 Hz, 2H), 7.92-7. 84 (m, 2H), 7.82- 7.76 (m, 2H), 7.62 (t, J = 8.3 Hz, 2H), 7. 59-7.49 (m, 2H), 7.45 (dd, J = 9.7 and 3.4 Hz, 2H), 7.37-7. 30 (m, 2H), 7.29-7. 21 (m, 2H), 7.16 (dd, J = 7.4 and 5.08 Hz, 2H), 6.86-6. 74 (m, 2H), 4.74-4. 66 (m, 2H), 3.48-3. 29 (m, 4H), 2.96 (s, 3H). MS (ESI) (M+I) + : 603.
HRMS (M+1) + Calcd for C25H23C1N604S3 : 603.0710 ; Found: 603.0806.
Example 22: 5-Chloro-6-[2-[[(10,11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-c] pyridin- 5-yl) ammo] carbonyl] hydrzino]-N-K3R)-l-methyt-2-oxo-3-pyrrolidinyl]-3- pyridinecarboxamide.
22A: 4-Methyl-3-(phenylethynyl)-pyridine.
All the liquid reagents were degassed with argon for 15-20 minutes. A mixture of phenylacetylene (110 u, L, 1 mmol, 3.6 eq. ), Pd (Ph3) 2Cl2 (24 mg, 0.03 mmol), CuCl (14 mg, 0.14 mmol) and Et3N (1 mL, 7.1 mmol) in DMF (1 ml) was stirred 15 minutes at room temperature. The yellow solution was then treated drop by drop with a solution of 3-bromo-4-methyl-pyridine (30 p. L, 0.28 mmol) in DMF (0.5 ml) and then refluxed at 150 °C for 3 hours. The resulting brown solution was cooled and concentrated under reduced pressure then diluted in CH2CI2 and washed successively with saturated aqueous NaHCO3, water, and brine, dried and concentrated under reduced pressure. The product was purified by column chromatography (ether-hexane) to obtain the title compound (35 mg, 65 %).'H NMR (400 MHz, CDCI3) : # 8.69 (bs, 1H), 8.38 (bs, 1H), 7.57-7. 51 (m, 2H), 7.39-7. 33 (m, 3H), 7.16 (bs, 1H), 2.50 (s, 3H) ppm. MS (ESI) (M+l) : 194.
22B: 4-Methvl-3- (2-phenylethyl)-pyridine.
To a solution of 4-methyl-3- (phenylethynyl)-pyridine in MeOH was added Pd/C and the mixture was stirred at room temperature for two days. The catalyst was then removed by filtration and the mixture concentrated under reduced pressure. The obtained . product was directly used for the next steps NMR (400 MHz, CDC13) : 8 8.30 (bs, 2H), 7.31-7. 14 (m, 3H), 7.15 (d, J = 7.2 Hz, 2H), 7.03 (bd, J = 4.1 Hz, 1H), 2.95-2. 80 (M, 4H), 2.23 (s, 3H) ppm. MS (ESI) (M+1) + : 198.
22C: 3- (2-Phenylethyl)-4-pvridinecarboxylic acid.
Following similar method reported in literature Villani, F. J. , Daniels, P. J. L., Ellis, C. A. , Mann, T. A. , Wang, K-C. J. Heterocyl. Chem. 1971,73-78 : SeO2 (4.8 g, 43. 24. mmol) was added to a solution of 4-methyl-3- (2-phenylethyl)-pyridine (3.28g, 16.62 mmol) in pyridine (21 mL) and the mixture was heated with stirring under reflux for 3 hours. Chloroform was then added and the mixture was filtered and then concentrated under reduced pressure. The residue was dissolved in dilute ammonium hydroxide and extracted with ether. The organic phase was discarded and the aq. phase was acidified with acetic acid and the product was allowed to crystallize. The acid was collected by filtration (2.3 g, 61 %). 1H NMR (400 MHz, CD30D): 8 8.37 (d, J = 5.1 Hz, 1H), 8.23 (s, 1H), 7.66 (d, J = 5.3 Hz, 1H), 7.17-7. 01 (m, 5H), 3.22-3. 10 (m, 2H), 2. 84-2. 76 (m, 2H) ppm. MS (ESI) (M+1) + : 228 22D: 10,11-Dihydro-5H-benzo[4,5]cyclohepta[1,2-c]pyridin-5-one.
PPA (96 g) was added to the 3- (2-phenylethyl)-4-pyridinecarboxylic acid (2.3 g, 10.12 mmol) and the mixture was heated at 170 °C for 2 hours. The solution was cooled to about 60 °C and ice water was added with stirring. The mixture was then made basic by the gradual addition of solid NaOH and the aq. phase was extracted several times with AcOEt. The combined organic layers were washed with brine, dried and concentrated under reduced pressure to obtain the product which was purified by a short chromatography column to provide the title compound (1.9 g, 90%). IH NMR (400 MHz, CDCl3) : # 8.63-8. 56 (m, 2H), 7. 99 (d, J = 8.0 Hz, 1H), 7.79 (d, J = 5.1 Hz, 1H), 7.46 (t, J = 7.1 Hz, 1H), 7.33 (t, J = 7.6 Hz, 1H), 7.24 (d, J = 7.6 Hz, 1H), 3.25-3. 15 (m, 4H) ppm.
MS (ESI) (M+1) + : 210.
22E: 10, 1 l-Dihydro-oxime, (5E)-5H-benzof4, 5lcycloheptarl, 2-cgpyridin-5-onew To a solution of 10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-c] pyridin-5-one (1.6 g, 7.65 mmol) in 70 mL pyridine was added hydroxylamine hydrochloride (3.5 g, 50.72 mmol) at rt. The mixture was then refluxed for 3 hours. More hydroxylamine hydrochloride 1.1 g (15.94 mmol) was added and the heating was continued overnight.
The mixture was then cooled and condensed under reduced pressure. Saturated NaHCO3 was then added and the aq. phase was extracted several times with CH2Cl2. The combined organic layers were washed with brine and then concentrated under reduced pressure to provide the oxime intermediate (1.40 g) which was used directly for the next step. MS (ESI) (M+1) + : 225.
22F: 10, 11-Dihydro-5H-benzo[4,5]cyclohepta[1,2-c]pyridin-5-amine.
A solution of 10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-c] pyridin-5-one oxime (1.4 g, 6.25 mmol) in EtOH/DMF (42/7 mL) was treated with Zn (2.1 g, 32. 30 mmol),
NH40H (28 mL) and NH40Ac (0.46 g, 5.97 mmol). The mixture was refluxed for one hour and then cooled to the room temperature and concentrated under reduced pressure.
The residue was then diluted in CH2Cl2 and washed with a saturated solution of NaHC03.
The aqeous phase was extracted several times with CH2Cl2. The combined organic phases were washed with brine, dried and concentrated under reduced pressure to provide 1.3 g of amine which was directly used for the next step.'H NMR (400 MHz, CDC13) : 8 8.26 (bd, J = 4.7, 1H), 8.16 (s, 1H), 7.30 (dd, J = 5.2, 3.6 Hz, 1H), 7.26 (d, J = 5.1 Hz, 1H), 7.14-7. 03 (m, 3H), 5.43 (s, 1H), 3.25-3. 15 (m, 2H), 3.15-3. 07 (m, 2H), 1.54 (bs, 2H) ppm.
MS (ESI) (M+1)+: 211.
22G: 5-Chloro-6-[2-[[(10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-c ]pyridin-5- yl) aminolcarbonvllhydrazinol-3-pyridinecarboxelic acid.
A solution of 10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-c] pyridin-5-amine (22 mg, 0.1 mmol) in DMF (0.7 ml) was added drop by drop to a stirred solution of CDI (16 mg, 0.1 mmol) in DMF (1 ml) at 0 °C and then allowed to warm to room temperature with stirring. After all the amine was consumed, 5-chloro-6-hydrazino-3- pyridinecarboxylic acid was added and stirring was continued for 2 hours. The mixture was then concentrated under reduced pressure and then diluted in CH2Cl2, washed with saturated NaHC03, water and brine, and then dried and concentrated. The dark-yellow residue was trituated with MeOH. The solid was collected and used directly for the next step. MS (ESI) (M+1) + : 424.
22H: 5-Chloro-6-[2-[[(10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-c ]pyridin-5- yl) amino1carbonyl] hydrazinol-N-[(31-methyl-2-oxo-3-pyrrolidinyll-3- pyridinecarboxamide.
HATU (29 mg, 0. 07 mmol) was added to a mixture 5-chloro-5-[2-[[(10,11- . dihydro-5H-benzo [4, 5] cyclohepta [1, 2-c] pyridin-5-yl) amino] carbonyl] hydrazino] -3- pyridinecarboxylic acid (25 mg, 0.06 mmol), (3R)-3-amino-1-methyl-2-pyrrolidinone, monohydrochloride (11 mg, 0.07 mmol) and DIPEA (24 L, 0.14 mmol) in 2 mL DMA.
The mixture was stirred at room temperature for 2 hours and then DMA was removed under reduced pressure. The residue was diluted in CH2C12 and washed successively with saturated NaHCO3, water, brine, dried and concentrated. The residue was then purified by flash chromatography (CH2CI2-MeOH) to provide the title compound (6 mg).'H NMR (400 MHz, CD30D): 8.44 (s, 1H), 8.15 (bs, 2H), 7.98 (s, 1H), 7.37 (d, J = 4.9 Hz, 1H), 7.25 (d, J = 7.0 Hz, 1H), 7.15-7. 01 (m, 2H), 6.41 (s, 1H), 4.59-4. 49 (m, 1H), 3. 35 (dd, J = 9.0, 4.7 Hz, 3H), 3.30-2. 97 (m, 3H), 2.79 (s, 3H), 2.42-2. 32 (m, 1H), 2.02-1. 87 (m, 1H), 1.20-1. 10 (m, 1H) ppm. MS (ESI) (M+1) + : 520.
Example 23: 5-chloro-6- [2- [ [ (10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-c] pyridin- 5-yl) aminolcarbonyllhydrazino]-N-[(3R)-tetrahydro-2-oXo-3-thienyl ]-3- pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1C: HATU (35 mg, 0.09 mmol) was added to a mixture of 5-Chloro-6-[2-[[(10,11-dihydro-5H- benzo [4,5] cyclohepta [1, 2-c] pyridin-5-yl) amino] carbonyl] hydrazino]-3- pyridinecarboxylic acid (0.08 mmol), D-homocysteine thiolactone. HCl (20 mg, 0.13 mmol) and DIEPA (31 µL, 0.18 mmol) in 2 mL DMA. The mixture was stirred at rt for 2 hours and then DMA was removed under reduced pressure. The residue was then diluted
in CH2C12 and washed with a saturated solution of NaHC03, water and a saturated solution of NaCl, dried and concentrated. The product was then purified by column chromatography (CH2Cl2-MeOH) to provide the title compound (6 mg).'H NMR (400 MHz, CD30D) 8 : 8.44 (s, 1H), 8. 23-8. 13 (m, 2H), 8.00 (s, 1H), 7.37 (d, J = 5.5 Hz, 1H), 7.25 (d, J = 7.0 Hz, 1H), 7.14-7. 02 (m, 2H), 6.41 (s, 1H), 4.59-4. 49 (m, 1H), 3. 35-2. 95 (m, 6H), 2.57-2. 47 (m, 1H), 2.25-2. 12 (m, 1H), 1.20 (t, J = 7.3 Hz, 1H) ppm. MS (ESI) (M+1) + : 523. HRMS (M+1) + Calcd for C25H23C1N603S : 532. 1319 ; Found: 523.1417.
Example 24: 5-chloro-6-l2-[[(6, 11-dihydro-5H-benzo [5,6] cycloheptat1, 2-c] pyridin- 11-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-1-methyl-2-oxo-3-pyrrolidinyl]- 3- pyridinecarboxamide..
24A: 5, 6-dihydro-11H-benzo[5,6]cyclohepta[1,2-c]pyridin-11-one, oxime. 2. 5 eq H2NOH. HCI N pyridine N J | 1 ootc N OH OH To a stirred solution of 5, 6-dihydro-1 lH-benzo [5,6] cyclohepta [1, 2-c] pyridin-11- one (2.7 g, 12.9 mmol) (prepared according to literature method described in Villani F. J. , et al, J. Heterocyclic Chem. 1971,8, 73, which is incorporated by reference herein for its disclosure of the preparation of 5, 6-dihydro-11H-benzo [5,6] cyclohepta [1, 2-c] pyridin-11- one) in pyridine (30 mL) at 100°C was added NH2OH. HCl (32.25 mmol, 2.24 g) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuo, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give product as pale yellow powder 2.74 g (yield 95%), which was used without further purification. MS (ESI) (M+H) + = 225.
24B: 6. 11-dihydro-5H-benzo[5,6]cyclohepta[1,2-c]pyridin-11-amine. 5 eq Zn powder 1 eq NH40Ac EtOH/DMF/28% NH40H reflux NH2 <Jn
To a mixture of 5, 6-dihydro-1 lH-benzo [5,6] cyclohepta [1, 2-c] pyridin-11-one oxime (2.24 g, 10 mmol) in EtOH (60 mL), DMF (10 mL) and 28% NH40H (40 mL) was added Zn powder (3.25 g, 50 mmol) followed by NH40Ac (0.77 g, 10 mmol). The mixture was stirred at reflux for 3 h. After cooled to room temperature, Et20 (200 mL) was added and stirred for 20 min, then IN NaOH (50 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et2O (3 x 50 mL). The combined organic layer was washed with water and then brine, dried over Na2S04, evaporated to give white solid 1.93 g (92%), which can be used without further purification. MS (ESI) (M+H) + = 211.
24C: 6, 11-dihydro-11-isothiocyanato-5H-benzo[5,6]cyclohepta[1,2-c]p yridine. CS2/EDC triethyl amine Nez THF NCS To a solution of 6, 11-dihydro-SH-benzo [5,6] cyclohepta [1, 2-c] pyridin-11-amine (210 mg, 1.0 mmol) in dry THF (12 mL) was added CS2 (0.60 ml, 10 mmol) at-10°C, then EDC (383 mg, 2.0 mmol) was added in one portion. After being stirred for 15 minutes, TEA (279 I1L, 2.0 mmol) was added dropwise. The mixture was allowed to slowly warm up to room temperature and stirred for 4 hours. After filtration, the filtrate was concentrated and the residue was taken up in ethyl acetate (20 mL), extracted with saturated NaHCO3 (1x10 mL), water (1x10 mL) and then brine (1x10 mL), dried over Na2SO4, then concentrated in vacuo. The residue was purified by passing through a short pad of silica gel (30% EtOAc in DCM) to give the title compound as a pale yellow oil (238 mg, 94%). MS (ESI) (M+H) + = 253.
24D: 5-chloro-6-[2-[[(6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-c] pyridin-11- ylBamino] thioxomethyl] hydrazinol-3-pyridinecarboxylic acid.
To a solution of 6, 11-dihydro-11-isothiocyanato-5H-benzo [5,6] cyclohepta [1, 2- c] pyridine (138 mg, 0.54 mmol) in DMA (3 mL) was added 5-chloro-6-hydrazino-3- pyridinecarboxylic acid (102 mg, 0.54 mmol), the mixture was stirred at room temperature for 2 hours, the solvent was concentrated in vacuo and water was added, the precipitate was collected by filtration, dried to give the title compound as off-white powders (225 mg, 94%). MS (ESI) (M+H) + = 440.
24E : 5-chloro-6-[2-[[(6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-c] pyridin-11- yl)amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-py rrolidinyl]-3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6-[2-[[(6, 11-dihydro-5H- benzo [5,6] cyclohepta [1, 2-c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-3- pyridinecarboxylic acid (265 mg, 0.6 mmol) in DMA (5 mL) was added HATU (276 mg, 0.72 mmol) followed by DIPEA (124 I1L, 0.72 mmol). The mixture was stirred at room temperature for 10 min, and then (3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride (108 mg, 0.72 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (50 mL), and washed with conc. NaHCO3 (2 x 20 mL) and then brine (1 x 20 mL). The residue obtained after removal of the solvent was purified by flash chromatography
(5#10% MeOH in DCM) to provide the title compound (106 mg, 33%).'H-NMR (400MHz, CD30D) : 8 8.54 (s, 1H), 8.52-8. 46 (m, 1H), 8. 19 (d, J = 5Hz, 1H), 8.06-7. 98 (m, 1H), 7. 44-7. 24 (m, 2H), 7. 23-7. 05 (m, 4H), 4. 73-4. 56 (m, 1H), 3. 48-3. 38 (m, 1H), 3. 31- 3.01 (m, 4H), 2.85 (s, 3H), 2.55-2. 36 (m, 1H), 2.13-1. 95 (m, 1H) ppm. MS (ESI) (M+1) += 536. HRMS (M+H)+ Calcd for C26H26ClN702S : 536.1635 ; Found : 536. 1736.
Example 25 : 5-chloro-6-[2-[[(6, 11-dihydro-5H-benzo [5, 6lcyclohepta [1, 2-c] pyridin- 11-yl) amino] thioxomethyl] hydrazinol-N- [ (3R)-tetrahydro-2-oxo-3-thienylj- 3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6-[2-[[(6, 11-dihydro-5H- benzo [5, 6] cyclohepta [1, 2-c] pyridin-11-yl) amino] thioxomethyl] hydrazino]- 3- pyridinecarboxylic acid (198 mg, 0.45 mmol) in DMA (4 mL) was added HATU (207 mg, 0.54 mmol) followed by DIPEA (93 µL, 0.54 mmol). The mixture was stirred at room temperature for 10 min, and then D-homocysteine thiolactone hydrochloride (83 mg, 0.54 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated. in vacuo, the residue was taken up in DCM (40 mL), and washed with conc. NaHCO3 (2 x 20 mL) and then brine (1 x 20 mL). The residue obtained after removal of the solvent was purified by flash chromatography (0#5% MeOH in DCM) to provide the title compound (108 mg, 45%).'H-NMR (400MHz, CD30D) : 8 8.58 (s, 1H), 8. 52-8. 48 (m, 1H), 8. 29-8.21 (m, 1H), 8. 13-8. 03 (m, 1H), 7.45-7. 32 (m, 2H), 7.22-7. 15 (m, 3H), 7. 13 (d, J= 5Hz, 1H), 4.93-4. 80 (m, 1H), 3.53- 3.40 (m, 1H), 3.37-3. 28 (m, 1H), 3.28-3. 06 (m, 4H), 2.68-2. 52 (m, 1H), 2.40-2. 21 (m, 1H) ppm. HRMS (M+H) + Calcd for C25H23ClN6O2S2 : 539.1091 ; Found: 539.1192.
Example 26: 5-chloro-6-[2-[[(6,11-dihydro-7-methyl-5H-benzo [5,6] cyclohepta [1,2- c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyll-3-pyridinecarboxamide.
26A : ethyl 4-f (E)-2- (2-methylphenyllethenyll-3-pyridinecarboxylate. if o oEt'o w o 1 N AcO heat OEt N
To a solution of ethyl 4-methylnicotinate (3.30 g, 20 mmol) in Ac20 (6 mL) was added o-tolualdehyde (4.81 g, 40 mmol) and the mixture was stirred at 140°C for 24h under N2. After cooled to room temperature, the mixture was concentrated in vacuo, water (12 mL) was added and the mixture was extracted with Et2O (3 x 20 mL), the combined organic solution was extracted with 5% HCI (12 mL), and the aqueous solution was then neutralized with 28% NH40H to pH >9, then extracted with Et20 (2 x 20 mL), dried over Na2S04 and concentrated. The crude product (a dark brown oil,-3. 8 g) was used directly for the next step without further purification. MS (ESI) (M+H) + = 268.
26B : ethyl 4-[2-(2-methylphenyl)ethyl]-3-pyridinecarboxylate.
The solution of ethyl 4-[(E)-2-(2-methylphenyl) ethenyl]-3-pyridinecarboxylate (#3. 8 g) dissolved in MeOH (20 mL) was hydrogenated at #40 psi for 3 h in the presence
of 10% Pd/C at room temperature. After work-up, the crude product was purified by passing through a short pad of silica gel (20% EtOAc in hexanes) to give the title compound as a light yellow oil (2.3 g, yield 42% for two steps). MS (ESI) (M+H) + = 270.
26C: 4-r2-(2-methylphenyl) (2-methylphenyl)ethyl]-3-pyridinecarboxylic acid.
Ethyl 4- [2- (2-methylphenyl) ethyl] -3-pyridinecarboxylate (2. 3 g, 8.5 mmol) was mixed with 12 mL of H2O/EtOH (1: 4). KOH (1.90 g, 34 mmol) was added and the mixture was stirred at reflux for 4h. After cooled to room temperature, most solvents were removed and water (8 mL) was added. The solution was carefully neutralized to pH 5#6, the precipitate was collected and dried to give the title compound as white powders (1.8 g, 88%). MS (ESI) (M+H) + = 242.
26D: 5, 6-dihydro-7-methyl-11H-benzo[5,6]cyclohepta[1,2-c]pyridin-11 -one. I polyphosphorcus acid heat O N/ --z : N N N 1.8 g of 4- [2- (2-Methylphenyl) ethyl]-3-pyridinecarboxylic acid (7.44 mmol) was added to stirred PPA (70 g) at 100°C arid the mixture was stirred at 160°C for another 6 h.
After cooling to 100°C, the mixture was poured into crashed ice (70 g) and the solution was carefully basified with KOH to pH >10. After extraction with EtOAc (4 x 40 mL), dried over Na2SO4 and evaporation, the title compound was obtained in 38% yield (0.63 g) and was used for the next step without further purification. (M+H) + = 224.
26E: 5, 6-dihydro-7-methyl-11H-benzo[5,6]cyclohepta[1,2-c]pyridin-11 -one, oxime. 2. 5 eq H2NOH. HCI N 1 PY N 0 100C N t\J pyridine N<J OH
To a stirred solution of 5, 6-dihydro-7-methyl-1 lH-benzo [5,6] cyclohepta [1, 2- c] pyridin-11-one (0.63 g, 2.84 mmol) in pyridine (4 mL) at 100°C was added H2NOH. HCl (0.49 g, 7.1 mmol) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuo, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give the title compound as pale yellow powders (0. 63g, 93%), which was used without further purification. MS (ESI) (M+H) + = 239.
26F: 6, dihvdro-7-methyl-SH-benzo [5, 6lcvGloheptaU12-clPvrídin amine. 5 eq Zn powder \ 1 eq NH40Ac, \ \C EtOH/DMFl28° ! o NH40H N N7 reflux NH2 OH To a mixture of 5, 6-dihydro-7-methyl-11H-benzo [5,6] cyclohepta [1, 2-c] pyridin- 11-one oxime (0.6 g, 2.5 mmol) in EtOH (15 mL), DMF (2.5 mL) and 28% NH40H (10 mL) was added Zn powder (0.82 g, 12.5 mmol) followed by NH40Ac (0.20 g, 2.5 mmol). The mixture was stirred at reflux for 3 h. After cooled to room temperature, Et20 (50 mL) was added and stirred for 20 min, then IN NaOH (15 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et20 (4 x 20 mL). The combined organic layer was washed with water and then brine, dried over Na2S04, evaporated to give the title compound as white solids (0.53 g, 94%), which was used without further purification.
MS (ESI) (M+H) + = 225.
26G: 6, 11-dihvdro-7-methyl-1l-isothiocvanato-5H-benzo (5, 6] cyclohepta [1, 2-c] pyridine. \ CS2/EDC N l triethyl amine NHZ THF NCS
To a solution of 6, 11-dihydro-7-methyl-5H-benzo [5,6] cyclohepta [1, 2-cupyridin- 11-amine (22.4 mg, 0. 1 mmol) in dry THF (1.2 mL) was added CSa (60 µL, 1.0 mmol) at -10°C, then EDC (38.2 mg, 0.2 mmol) was added in one portion. After being stirred for 15 minutes, TEA (28 µL, 0.2 mmol) was added dropwise. The mixture was allowed to slowly warm up to room temperature and stirred for 4 hours. After filtration, the filtrate was concentrated and the residue was taken up in ethyl acetate (10 mL), extracted with saturated NaHCO3 (1x10 mL), water (1x10 mL) and then brine (1x10 mL), dried over Na2S04, then concentrated in vacuo. The residue was purified by passing through a short pad of silica gel (30% EtOAc in DCM) to give the title compound as white solids (21 mg, 79%). MS (ESI) (M+H) + = 267.
26H : 5, 6-dichloro-N-[(3R)-1-methyl-2-oxo-3-pyrrolidinyl]- 3-pyridinecarboxamide.
Following general HATU coupling procedure of Example 1 C : The title compound (108 mg, 74%) was prepared from 5,6-dichloronicotic acid and (3R)-amino-1-methyl-2- pyrrolidinone HCl salt. 1HNMR (DMSO-d6) : # 9.07 (d, J = 8.2 Hz, 1H), 8.76 (s, 1H), 8.45 (s, 1H), 4.55 (q, J = 9.0 Hz, 1H), 3. 25-3.35 (m, 2H), 2.73 (s, 3H), 2.25-2. 35 (m, 1H), 1.85-1. 95 (m, 1H) ppm. MS (ESI) (M+1) + : 289.
26I : 5-chloro-6-hydrazino-N= [(3R)-1-methyl-2-oxo-3-pyrrolidinvll- 3- pvridinecarboxamide.
1 M NH2NH2 in EtOH (1 ml) was added to a solution of 5, 6-dichloro-N-[(3R)-l-methyl- 2-oxo-3-pyrrolidinyl]-3-pyridinecarboxamide (98 mg, 0. 34mmol) in EtOH (2 ml), the . reaction mixture was heated at reflux for 8 h, allowed to cool to room temperature. The white solid was collected and washed with MeOH (3 ml) to afford the title compound (66 mg).
26J: 5-chloro-6-[2-[[(6,11-dihydro-7-methyl-5H-benzo[5,6]cyclohep ta[1,2-c]pyridin-11- yamino] thioxomethYl] hvdrazino]-N- [ (3R)-1-methvl-2-oxo-3-pyrrolidinvll-3- pyridinecarboxamide.
To a stirred solution of 6, 11-dihydro-7-methyl-11-isothiocyanato-5H- benzo [5, 6] cyclohepta [1, 2-c] pyridine (21 mg, 0.08 mmol) in DMA (2 mL) was added 5- chloro-6-hydrazino-N- [ (3R)-1-methyl-2-oxo-3-pyrrolidinyl]-3-pyridinecarboxamide (22.7 mg, 1.0 mmol). The mixture was stirred at room temperature for 2 hours and then concentrated in vacuo, the residue was purified by flash chromatography (5-10% MeOH in DCM) to provide the title compound (15 mg, 34%). 1H-NMR (400Mhz, CD3OD) : 8 8.60-8. 55 (m, 1H), 8. 52 (s, 1H), 8.27-8. 21 (m, 1H), 8. 10 (d, J = 2Hz, 1H), 7.40 (d, J = 2Hz, 1H), 7.19 (d, J = 2Hz, 1H), 7.14 (d, J = 2Hz, 1H), 7. 11-6. 97 (m, 2H), 4.73-4. 60 (m, 1H), 3.52-3. 39 (m, 2H), 3.34-3. 27 (m, 4H), 2.88 (s, 1H), 2.54-2. 42 (m, 1H), 2. 12-1. 96 (m, 1H) ppm. MS (ESI) (M+1)+=550. HRMS (M+H) + Calcd for C27H28ClN702S : 550.1792 ; Found: 550.1763. Example 27: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cyclohepta [1, 2- c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3-pyridinecarboxamide.
27A: ethyl 4-[(E)-2-(2-fluorophenyl)ethenyl]-3-pyridinecarboxylate. if F I OEt AC20-___ 0 ARCHE N heat I OEt J N'
To a solution of ethyl 4-methylnicotinate (8.25 g, 50 mmol) in AC20 (16 mL) was added 2-flurobenzaldehyde (12.4 g, 100 mmol) and the mixture was stirred at 140°C for 24h under N2. After cooled to room temperature, the mixture was concentrated in vacuo, water (35 mL) was added and the mixture was extracted with Et20 (3 x 40 mL), the combined organic solution was extracted with 5% HCl (30 mL), and the aqueous solution was then neutralized with 28% NH40H to pH >9, then extracted with Et20 (2 x 40 mL), dried over Na2SO4 and concentrated. The crude product (a dark brown oil,-14 g) was used directly for the next step without further purification. MS (ESI) (M+H) + = 274.
27B: ethyl 4-[(2-(2-fluorophenyl)ethyl]-3-pyridinecarboxylate.
The solution of ethyl 4-[(E)-2-(2-fluorophenyl) ethenyl]-3-pyridinecarboxylate (~14 g) dissolved in MeOH (50 mL) was hydrogenated at-40 psi for 3 h in the presence of 10% Pd/C at room temperature. After work-up, the crude product was purified by passing through a short pad of silica gel (20% EtOAc in hexanes) to give the title compound as a light yellow oil (7.2 g, yield 53% for two steps).'H-NMR (400MHz, CDCl3) : 8 9.08 (s, 1H), 8.54 (d, J = 5 Hz, 1H), 7.25-6. 93 (m, 5H), 4.40 (q, J = 8 Hz, 2H), 3.36-3. 19 (m, 2H), 3.02-2. 81 (m, 2H), 1.41 (t, J = 8Hz, 3H) ppm. MS (ESI) (M+H) += 272.
27C: 4-[2-(2-fluorophenyl)ethyl]-3-pyridinecarboxylic acid. W F KOH L KOH t EtOHIH20 reflux OEt I OH "N" N N Ethyl 4- [2- (2-fluorophenyl) ethyl] -3-pyridinecarboxylate (7.1 g, 26 mmol) was mixed with 35 mL of H20/EtOH (1: 4). KOH (5.83 g, 4 eq) was added and the mixture was stirred at reflux for 4h. After cooled to room temperature, most solvents were removed and water (30 mL) was added. The solution was carefully neutralized to pH 5-6, the precipitate was collected and dried to give the title compound as white powders 6.3 g (yield 99%). MS (ESI) (M+H) + = 246.
27D: 7-fluoro-5, 6-dihydro-11H-benzo[5,6]cyclohepta[1,2-c]pyridin-11-one. if "I polyphosphorous acid heart \ 0 nô,' 0 oh Fizz
6. 3 g of 4- [2- (2-Fluorophenyl) ethyl]-3-pyridinecarboxylic acid (25.7 mmol) was . added to stirred PPA (240 g) at 100°C and the mixture was stirred at 160°C for another 6 h. After cooling to 100°C, the mixture was poured into crashed ice (200 g) and the solution was carefully basified with KOH to pH >10. After extraction with EtOAc (4x150 mL), dried over Na2S04 and evaporation, the title compound was obtained in 92% yield (5.38 g) and was used for the next step without further purification. MS (ESI) (M+H) + 228.
27E: 7-fluoro-5, 6-dihydro-11H-benzo[5,6]cyclohepta[1,2-c]pyridin-11-one. oxime. 2. 5 eq F ; 4 H2NOH. HCI O pyridine NS OH OH To a stirred solution of 7-fluoro-5, 6-dihydro-1 lH-benzo [5, 6] cyclohepta [1, 2- c] pyridin-11-one (5. 38 g, 23.7 mmol) in pyridine (30 mL) at 100°C was added H2NOH. HC1 (4.12 g, 59.3 mmol) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuum, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give the title compound as pale yellow powders (5. 51g, yield 96%), which was used without further purification. MS (ESI) (M+H) + = 243.
27F: 7-fluoro-6, 11-dihydro-5H-benzo[5,6]cyclohepta[1,2-c]pyridin-11-amine. F F 5 eq Zn powder \ 1 eq NH40Ac -/ OH EtOH/DMF/28°o NH40H N reflux nez Un
To a mixture of 7-fluoro-5,6-dihydro-11H-benzo[5, 6] cyclohepta [1, 2-c] pyridin-11- one oxime (1.21 g, 5 mmol) in EtOH (30 mL), DMF (5 mL) and 28% NH40H (20 mL) was added Zn powder (1.63 g, 25 mmol) followed by NH40Ac (0.39 g, 5 mmol). The mixture was stirred at reflux for 3 h. After cooled to room temperature, Et20 (100 mL) was added and stirred for 20 min, then 1N NaOH (30 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et20 (4 x 50 mL). The combined organic layer was washed with water and then brine, dried over Na2S04, evaporated to give the title compound as white solids (1.07 g, 94%), which was used without further purification.
'H-NMR (400MHz, CDCl3) : 5 8.55 (s, 1H), 8.39 (d, J = 5 Hz, 1H), 7.28-7. 16 (m, 2H), 7.05 (d, J = 5 Hz, 1H), 7.00-6. 91 (m, 1H), 5.40 (s, 1H), 3.69-3. 46 (m, 2H), 3.21-2. 93 (m, 2H), 1. 85 (s, br, 2H) ppm. MS (ESI) (M+H) + = 229.
27G: 7-fluoro-6, 11-dihydro-11-isothicoyanato-5H-benzo[5,6]cyclohepta[1,2-c]p yridine, F F CS2/EDC N triethyl amine N NH2 THF NCS To a solution of 7-fluoro-6, 11-dihydro-SH-benzo [5, 6gcycloheptar1, 2-c] pyridin- 11-amine (571 mg, 2.5 mmol) in dry THF (30 mL) was added CS2 (1-5 mol, 25 mmol) at -1 10°C, then EDC (956 mg, 5.0 mmol) was added in one portion. After being stirred for 15 minutes, TEA (697 WL, 5. 0 mmol) was added dropwise. The mixture was allowed to slowly warm up to room temperature and stirred for 4 hours. After filtration, the filtrate was concentrated and the residue was taken up in ethyl acetate (50 mL), extracted with saturated NaHC03 (1x20 mL), water (1x20 mL) and then brine (1x20 mL), dried over Na2S04, then concentrated in vacuo. The residue was purified by passing through a short pad of silica gel (30% EtOAc in DCM) to give the title compound as white solids (578 mg, 86%). 1H-NMR (400MHz, CDCl3) : 8 8.62 (s, 1H), 8.49 (d, J = 5 Hz, 1H), 7. 25-7. 18 (m, 2H), 7.12 (d, J = 5 Hz, 1H), 7. 08-7. 02 (m, 1H), 6.22 (s, 1H), 3. 48-3. 35 (m, 2H), 3. 18-3. 05 (m, 1H) ppm. MS (ESI) (M+H) += 271.
27H : 5-chloro-6-[2-[[(7-fluoro-6,11-dihydr-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl) aminolthioxomethyllhydrazinol- 3-pyridinecarboxylic acid.
To a solution of 7-fluoro-6, 11-dihydro-11-isothiocyanato-5H- benzo [5, 6] cyclohepta [1, 2-c] pyridine (270 mg, 1.0 mmol) in DMA (5 mL) was added 5- chloro-6-hydrazino-3-pyridinecarboxylic acid (188 mg, 1.0 mmol), the mixture was stirred at room temperature for 2 hours, the solvent was concentrated in vacuo and water was added, the precipitate was collected by filtration, dried to give the title compound as off-white powders (428 mg, 93%). MS (ESI) (M+H) 458.
27I : 5-chloro-6-[2-[[(7-fluoro-6,11-dihydr-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-pyrro lidinyl]- 3- pyridinecarboxamide Method 271 (A): To a stirred solution of 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H- benzo [5, 6] cyclohepta [1, 2-c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-3- pyridinecarboxylic acid (69 mg, 0.15 mmol) in DMA (2 mL) was added HATU (69 mg, 0.18 mmol) followed by DIPEA (31 1L, 0.18 mmol). The mixture was stirred at room temperature for 10 min, and then (3R)-3-amino-l-methyl-2-pyrrolidinone hydrochloride (27 mg, 0. 18 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with conc. NaHC03 (2 x 10 mL) and then brine (1 x 10 mL). The
residue obtained after removal of the solvent was purified by flash chromatography (5~10% MeOH in DCM) to provide the title compound (43 mg, 52%).'H-NMR (400MHz, CD30D) : # 8.60-8. 46 (m, 2H), 8.24 (d, J = 5Hz, 1H), 8.09-7. 98 (m, 1H), 7.46- 7. 24 (m, 1H), 7.26-7. 05 (m, 3H), 4.71-4. 55 (m, 1H), 3. 52-3. 33 (m, 2H), 3.24-3. 03 (m, 4H), 2. 85 (s, 3H), 2.54-2. 36 (m, 1H), 2.13-1. 92 (m, 1H) ppm. HRMS (M+H) + Calcd for C26H2sClFN702S : 554.1541 ; Found: 554. 1548.
Method 271 (B): To a stirred solution of 7-fluoro-6, 11-dihydro-11-isothiocyanato-5H- benzo [5,6] cyclohepta [1, 2-c] pyridine (27 mg, 0.1 mmol) in DMA (2 mL) was. added 5- chloro-6-hydrazino-N- [ (3R)-l-methyl-2-oxo-3-pyrrolidinyl]-3-pyridinecarboxamide (28.4 mg, 1.0 mmol). The mixture was stirred at room temperature for 2 hours and then concentrated in vacuo, the residue was treated with small amount of MeOH and diethyl ether, the precipitate was collected by filtration and then dried to give the title compound as white solid (54 mg, 97%).
Examples 28 and 29 The two diastereoisomers of Example 27 were produced via chiral separation from compound 27 using semi-preparative column (Chiralpak AD 21 x 250 mm column), with 23% isopronaol/77% hexane (containing 0. 1% diethyl amine in each solvent) as a mobile phase (flow rate 9 ml/min, run of 120 min). Two diastereomers were successfully isolated: Example 28: Isomer A (earlier fraction) :'H-NMR (400MHz, CD30D) : 8 8.45 (d, J = 8Hz, 2H), 8.18 (d, J-SHz, IH), 7.98 (s, 1H), 7. 43-7. 32 (m, 1H), 7.26-7. 08 (m, 3H), 6.87 (t, J = 8Hz, 1H), 4.56 (t, J = IOHz, IH), 3.47-3. 38 (m, 2H), 3.24-3. 07 (m, 4H), 2.79 (s, 3H), 2.51-2. 40 (m, 1H), 2.10-2. 96 (m, 1H) ppm. [a] 25D+ 6. 6° (c 0. 098, methanol).
Example 29: Isomer B (later fractions) :'H-NMR (400MHz, CD30D): 8 8. 45 (s, 2H), 8.18 (d, J = 5Hz, 1H), 7.99-7. 97 (m, 1H), 7.27 (s, 1H), 7.19-7. 00 (m, 3H), 6.88 (t, J = 8Hz, 1H), 4.56 (t, J = lOHz, 1H), 3. 39-3. 30 (m, 2H), 3.16-2. 99 (m, 4H), 2.79 (s, 3H), 2.44-2. 31 (m, 1H), 2.04-1. 88 (m, 1H) ppm. [a] 25D-1. 2° (c 0.102, methanol).
Example 30: 5-chloro-6- [2- [ [ (7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta [1, 2- cl pyridin-11-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3- thienyl]-3-pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1 C : To a stirred solution of 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo [5,6] cyclohepta [1, 2- c]pyridin-11-yl) amino] thioxomethyl] hydrazino]- 3-pyridinecarboxylic acid (105 mg, 0.23 mmol) in DMA (3 mL) was added HATU (107 mg, 0.28 mmol) followed by DIPEA (48 pL, 0.28 mmol). The mixture was stirred at room temperature for 10 min, and then D- homocysteine thiolactone hydrochloride (43 mg, 0.28 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with cone. NaHCO3 (2 x 10 mL) and then brine (1 x 10 mL). The residue obtained after removal of the solvent was purified by flash chromatography (0-5% MeOH in DCM) to provide the title compound (44 mg, 34%). 1H-NMR (400MHz, CD3OD) : 6 8.61-8. 50 (m, 2H), 8. 34-8.25 (m, 1H), 8.13-8. 04 (m, 1H), 7.40 (s, br, 1H), 7.28-7. 20 (m, 2H), 7. 19-7.11 (m, 1H), 7.03-6. 94 (m, 1H), 4.92-4. 79 (m, 1H), 3.52-3. 40 (m, 1H), 3.36-3. 26 (m, 1H), 3.26-3. 08 (m, 4H), 2.71- 2.56 (m, 1H), 2.40-2. 22 (m, 1H) ppm. MS (ESI) (M+l) +-557. HRMS (M+H) + Calcd for C25H22CIFN602S : 557.0996 ; Found: 557.0910. Anal. Calcd for C2sH22C1FN602S. 2.3H2O : C, 50.17 ; H, 4.48 ; N, 14.04 ; Found: C, 50.28 ; H, 4.77 ; N, 14.07.
Example 31: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo [5,6] cycloheptal1, 2- cjpyridin-11-yl) aminolthioxomethyllhydrazino]-N- [ (3R)-2-oxo-l-phenyl-3- pyrrolidinyl]-3-pyridinecarboxamide.
To a stirred solution of 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H- benzo [5,6] cyclohepta [1, 2-c] pyridin-11-yl)amino]thioxomethyl]hydrazino]- 3- pyridinecarboxylic acid (92 mg, 0.2 mmol) in DMA (3 mL) was added HATU (92 mg, 0.24 mmol) followed by DIPEA (41 µL, 0.24 mmol). The mixture was stirred at room temperature for 10 min, and then (3R)-3-amino-1-phenyl-2-pyrrolidinone hydrochloride (51 mg, 0.24 mmol) (made according to the procedure disclosed in Ian M. Bell et al., J.
Med. Chem. , 2001,44, 2933, which is incorporated by reference herein for its disclosure in the preparation of (3R)-3-amino-1-phenyl-2-pyrrolidinone hydrochloride) was added.
The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with concentrated NaHC03 (2 x 10 mL) and then brine (1 x 10 mL). The residue obtained after removal of the solvent was purified by flash chromatography (0-5% MeOH in DCM) to provide the title compound (59 mg, 48%). 1H-NMR (400MHz, CD3OD) : 8 8.62-8. 57 (m, 1H), 8.54 (s, 1H), 8.28-8. 24 (m, 1H), 8.08 (d, J = 2Hz, 1H), 7.64 (d, J = 8Hz, 2H), 7.42- 7.38 (m, 3H), 7.26-7. 10 (m, 4H), 6.97 (t, J = 8 Hz, 1H), 4.94-4. 84 (m, lI-), 3.99-3. 82 (m, 2H), 3.27-3. 07 (m, 4H), 2.65-2. 53 (m, 1H), 2.29-2. 15 (m, 1H) ppm. MS (ESI) (M+l) + 616. HRMS (M+H) + Calcd for C3, H27ClFN702S : 616. 1698; Found: 616.1691. Anal.
Calcd for C31H27CIFN702S. 2H20 : C, 57.09 ; H, 4.79 ; N, 15.03 ; Found: C, 56.98 ; H, 4.72 ; N, 14.94.
Example 32: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo [5, 6] cyclohepta [1, 2- c] pyridin-ll-yl) amino] thioxomethyl] hydrazino]-N- [ [ (2R)-tetrahydro-2- furanyl] methyl]-3-pyridinecarboxamide.
Following the general HATU coupling procedure of Example 1C : HATU (50 mg) was added to a solution of 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H- benzo [5,6] cycloheptal1, 2-c] pyridin-11-yl)amino]thioxomethyl]hydrazino]-3- pyridinecarboxylic acid (52 mg), (2R)-tetrahydro-2-furanmethanamine (15 mg) and DIPEA (0.1 ml) in DMA (3 ml), and the reaction mixture was stirred at room temperature for 2 h. DMA was stripped off in vacuo and the residue was treated with H20 and the solid was collected and purified by prep-HPLC to give the title compound (15 mg) as a TFA salt.'H NMR (400MHz, CD30D) 8 : 8.83 (s, 1H), 8. 61 (d, J=6 Hz, 1H), 8.57 (s, 1H), 8.14 (s, 1H), 7. 89 (d, J=6Hz, 1H), 7.43 (br, 1H), 7.20-7. 33 (m, 2H), 7.05-7. 10 (m, 1H), 4.05-5. 15 (m, 1H), 3.89 (dd, J =7.2, 14.5 Hz, lH), 3.79 (dd, J = 10.0, 14.5 Hz, 1H), 3. 30- 3.60 (m, 5H), 3.10-3. 20 (m, 1H), 1.98-2. 08 (m, 1H), 1.88-1. 97 (m, 1H), 1.59-1. 70 (m, 1H) ppm. MS (ESI) (M+1) += 541. HRMS (M+1) + Calcd for C26H26C1FN602S : 541.1589 ; Found: 541. 1669.
Example 33: 5-chloro-6- [2- [[(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cyclohepta [1, 2- c] pyridin-1 1-yl) amino] carb onyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-thienyll- 3- pyridinecarboxamide.
33A: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohep ta[1,2-c]pyridin-11- Yl) aminolcarbonyllhydrazinol-3-pyridinecarboxylic acid.
To a solution of CDI (24 mg, 0.15 mmol) in dry DMF (1.5 mL) was added a solution of 7-fluoro-6, 11-dihydro-5H-benzo [5, 6] cyclohepta [1, 2-c] pyridin-11-amine (34.5 mg, 0.15 mmol) in dry DMF (1.5 mL) at 0°C. After being stirred for 1 hour, the mixture was allowed to warm up to room temperature and 5-chloro-6-hydrazino-3- pyridinecarboxylic acid (28 mg, 0.15 mmol) was added. The mixture was stirred at room temperature for another 2 hours. The solvent was concentrated in vacuo and the residue was treated with small amount of MeOH and diethyl ether, the white solid was collected and dried to afford the title comound (51 mg, 77%). MS (ESI) (M+H) + = 442.
33B: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohep ta[1,2-c]pyridin-11- yl)amino]carbonyl]hydrazino]-N-[(3R)-tetrahydro-2-oxo-3-thie nyl]- 3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6- [2- [ [ (7-fluoro-6, 11-dihydro-5H- benzo [5,6] cyclohepta [1, 2-c] pyridin-11-yl) amino] carbonyl] hydrazino]- 3- pyridinecarboxylic acid (51 mg, 0.115 mmol) in DMA (2 mL) was added HATU (54 mg, 0.14 mmol) followed by DIPEA (24 uL, 0.14 mmol). The mixture was stirred at room temperature for 10 min, and then D-homocysteine thiolactone hydrochloride (22 mg, 0.14 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with cone. NaHC03 (2 x 10 mL) and then brine (1 x 10 mL). The residue
obtained after removal of the solvent was purified by flash chromatography (0-5% MeOH in DCM) to provide the title compound (15 mg, 24%).'H-NMR (400MHz, CD30D): # 8.46 (s, 1H), 8.43-8. 39 (m, 1H), 8.20 (d, J = 5 Hz, 1H), 7.95 (s, 1H), 7.20-7. 04 (m, 3H), 6.91 (t, J = 8Hz, 1H), 6.28 (s, 1H), 4.86-4. 72 (m, 1H), 3.42-3. 30 (m, 1H), 3.28- 3.00 (m, 5H), 2.52-2. 47 (m, 1H), 2.28-2. 12 (m, 1H) ppm. MS (ESI) (M+1)+=541. HRMS (M+H) + Calcd for C25H22ClFN603S : 541.1225 ; Found: 541.1207.
Example 34: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cyclohepta [1, 2- clpyridin-ll-yl) amino] carbonyljhydrazino]-N- [ (3R)-l-methyl-2-oxo-3-pyrrolidinyll- 3-pyridinecarboxamide.
To a solution of CDI (31 mg, 0.19 mmol) in dry DMF (2 mL) was added a solution of 7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta 1, 2-c]pyridin-11-amine (44 mg, 0.19 mmol) in dry DMF (2 mL) at 0°C. After being stirred for I hour, the mixture was allowed to warm up to room temperature and 5-chloro-6-hydrazino-N-[(3R)-1- methyl-2-oxo-3-pyrrolidinyl]-3-pyridinecarboxamide (54 mg, 0.19 mmol) was added.
The mixture was stirred at room temperature for another 2 hours. The solvent was concentrated in vacuo, the residue was taken up into DCM (20 mL) and washed with water (lx 10 mL), dried over NazS04, concentrated and purified by flash chromatography (5-10% MeOH in DCM) to give the title compound as white solids (54 mg, 53%). 1h- NMR (400MHz, CD30D) : 8 8.53 (s, 1H), 8.46-8. 42 (m, 1H), 8. 28-8. 30 (m, 1H), 7.91 (d, J = 2Hz, 1H), 7.22 (d, J = 8Hz, 1H), 7. 18-7. 08 (m, 2H), 6.94 (t, J = 8Hz, 1H), 6.32 (d, J = 2Hz, 1H), 4. 63 (t, J = 8Hz, 1H), 3.44-3. 36 (m, 2H), 3.31-3. 23 (m, 2H), 3.16-3. 00 (m, 2H), 2.84 (s, 3H), 2.50-2. 36 (m, 1H), 2.09-1. 90 (m, 1H) ppm. MS (ESI) (M+1) +=538. HRMS (M+H) + Calcd for C2sH2sClFNO3 : 538.1770 ; Found : 538.1729.
Example 35: 5-chloro-6-[2-[[ (10, 11-dihydro-5H-benzo [4, 5]cyclohepta[1,2-b]pyridin- 5-yl) amino] thi6xomethyl] hydrazino]-N-K3R)-l-niethyl-2-oxo-3-pyrroIidmyl]- 3- pyridinecarboxamide.
35A: 10, 11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin-5-one, oxime.
25eq N +} H2NOH. HCI ¢ - //. pyridine OH OH OH To a stirred solution of 10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-b] pyridin-5- one (837 mg, 4.0 mmol) (prepared according to Villani F. J. , et al, J. Heterocyclic Chem., 1971,8, 73) in pyridine (10 mL) at 100°C was added H2NOH. HCl (10 mmol, 695 mg) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuo, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give product as pale yellow powder (843 mg, 94%), which was used without further purification. MS (ESI) (M+H) + = 225.
35B: 10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin-5-amine. 5 eq Zn powder 1 eq NH40Ac ll EtOH/DMF/28% NH40H A NH reflux z To a mixture of 10, 11-dihydro-5H-benzo [4, 5]cyclohepta[1,2-b]pyridin-5-one oxime (843 mg, 3.76 mmol) in EtOH (23 mL), DMF (3 mL) and 28% NH40H (15 mL) was added Zn powder (1.22 g, 18. 8 mmol) followed by NH40Ac (0 29 g, 3.76 mmol).
The mixture was stirred at reflux for 3 h. After cooled to room temperature, Et20 (80 mL) was added and stirred for 20 min, then IN NaOH (20 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et2O (3 x 20 mL). The combined organic layer was washed with water and then brine, dried over Na2S04, evaporated to give the title compound as a white solid (700 mg, 88%), which was used without further purification.
MS (ESI) (M+H) + = 211.
35C: 10, 11-dihydro-5-isothiocyanato-SH-benzoC4, 51cyclohepta [1, 2-bl p'. CS2/EDC triethyl amine NH2 THF NCS To a solution of 10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-b] pyridin-5-amine (210 mg, 1.0 mmol) in dry THF (12 mL) was added CS2 (601 LL, 10 mmol) at-10°C, then EDC (383 mg, 2.0 mmol) was added in one portion. After being stirred for 15 minutes, TEA (279 pL, 2.0 mmol) was added dropwise. The mixture was allowed to slowly warm up to room temperature and stirred for 4 hours. After filtration, the filtrate was concentrated and the residue was taken up in ethyl acetate (20 mL), extracted with saturated NaHCO3 (1x10 mL), water (1x10 mL) and then brine (1x10 mL), dried over Na2SO4, then concentrated in vacuo. The residue was purified by passing through a short pad of silica gel (30% EtOAc in DCM) to give the title compound as a pale yellow oil (230 mg, 91%). MS (ESI) (M+H) +=253.
35D: 5-chloro-6-[2-[[(10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b ]pyridin-5- vl) arrazino]-3-pyridinecarboxylic acid.
To a solution of 10, 11-dihydro-5-isothiocyanato-5H-benzo [4,5] cyclohepta [1, 2-b] pyridine (128 mg, 0.5 mmol) in DMA (3 mL) was added 5-chloro-6-hydrazino-3- pyridinecarboxylic acid (94 mg, 0.5 mmol), the mixture was stirred at room temperature for 2 hours, the solvent was concentrated in vacuo and water was added, the precipitate was collected by filtration, dried to give the title compound as off-white powders (211 mg, 96%). MS (ESI) (M+H) + = 440.
35E : 5-chloro-6-[2-[[(10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b ]pyridin-5- yl)amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-py rrolidinyl]- 3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6-[2-[r (10, 11-dihydro-5H- benzo [4,5] cyclohepta [1, 2-b] pyridin-5-yl) amino] thioxomethyl] hydrazino]-3- pyridinecarboxylic acid (88 mg, 0.2 mmol) in DMA (2 mL) was added HATU (92 mg, 0.24 mmol) followed by DIPEA (41 µL, 0.24 mmol). The mixture was stirred at room temperature for 10 min, and then (3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride (36 mg, 0. 24 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with concentrated NaHCO3 (2 x 10 mL) and then brine (1 x 10 mL).
The residue obtained after removal of the solvent was purified by preparative TLC (8% MeOH in DCM) to provide the title compound (23 mg, 21%). 1H-NMR (400MHz, CD30D) : 8 8.57 (s, 1H), 8.33-8. 25 (m, 1H), 8.17-8. 09 (m, 1H), 7.87 (d, J = 5Hz, 1H), 7.37 (s, br, 1H), 7.33 (d, J = 5Hz, 1H), 7.24-7. 10 (m, 4H), 4.71-4. 60 (m, 1H), 3.52-3. 41 (m, 2H), 3.39-3. 06 (m, 4H), 2. 89 (s, 3H), 2.56-2. 44 (m, 1H), 2. 13-2. 04 (m, 1H) ppm. HRMS (M+H) + Calcd for C26H26ClN702S : 536.1635 ; Found : 536.1689.
Example 36: 5-chloro-6-[2-[[(9-fluoro-10,11-dihydro-5H-benzo[4, 5] cyclohepta [1, 2- b] pyridin-5-yl) amino] carbonyl] hydrazino]-N-[(3R)-l-methyl-2-oxo-3-pyrrolidinyl]- 3-pyridinecarboxamide.
36A : 2-r (E)-2- (2-fluorophenvl) ethenyll- 3-p, vridinecarboxylic acid, sodium salt.
The mixture of NaH (2.64 g, 60% in mineral oil, 110 mmol), t-BuOH (8.62 mL, 90 mmol) in DMF (60 mL) was stirred at room temperature for 10 minutes and then warm up to 50°C until gas evolution ceased. The mixture was cooled to 0°C and a solution of ethyl 4-methylnicotinate (5. D g, 30 mmol) in DMF (10 mL) was added. After being stirred at the same temperature for 1 hour, 2-fluorobenzaldehyde (3.86 mL, 36 mmol) in DMF (10 mL) was added and the mixture was warmed up to room temperature and stirred for overnight. After being concentrated, the residue was used for the next step without further purification. MS (ESI) (M+H) + = 244.
36B: 2- [2- (2-fluorophenyllethvll- 3-pyridinecarboxvlic acid, sodium salt.
The above crude sodium salt of 2-[(E)-2-(2-fluorophenyl)ethenyl]- 3- pyridinecarboxylic acid was dissolved in MeOH (100 mL) and the mixture was hydrogenated at ~40 psi for 3 h in the presence of 10% Pd/C at room temperature. After filtration, the filtrate was concentrated dried in vacuo and the solid was used for the next step without further purification. MS (ESI) (M+H) + = 246.
36C : 9-fluoro-10, 11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin-5-one. i ont heart- beat 0 I,- Nay ZONA To the above crude sodium salt of 2- [2- (2-fluorophenyl) ethyl]-3- pyridinecarboxylic acid was added PPA (150 g) and the mixture was stirred at 160°C for 6 h. After cooling to 100°C, the mixture was poured into crashed ice (100 g) and the solution was carefully basified with KOH to pH >10. After extraction with EtOAc (4x 80 mL), dried over Na2S04 and evaporation, the title compound was obtained as pale yellow solids (3.2g, 47% for 3 steps) and was used for the next step without further purification.
MS (ESI) (M+H) + = 228.
36D: 9-fluoro-10, 11-dihydro-SH-benzo [4*51cycloheptarl, 2-blpyridin-5-one oxime. F 2. 5 ex N H2NOH. HCI 1 \ r PY--. I i oc OH
To a stirred solution of 9-fluoro-10, 11-dihydro-5H-benzo [4, 5] cyclohepta [1, 2-b] pyridin-5- one (1. 98 g, 8. 68 mmol) in pyridine (10 mL) at 100°C was added H2NOH. HC1 (1.51 g, 21.7 mmol) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuum, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give the title compound as pale yellow powders (2.05 g, 98%), which was used without further purification. MS (ESI) (M+H) + = 243.
36E : 9-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin -5-amine, F F 5 eq Zn powder /\ 1 eq NH40Ac I EtOHlDMF/28% NH40H OH reflux Un To a mixture of 9-fluoro-10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-b] pyridin- 5-one oxime (2.05 g, 8.48 mmol) in EtOH (18 mL), DMF (8 mL) and 28% NH40H (32 mL) was added Zn powder (2.76 g, 42.3 mmol) followed by NH40Ac (0.66 g, 8.48 mmol). The mixture was stirred at reflux for 3 h. After cooled to room temperature, EtzO (120 mL) was added and stirred for 20 min, then IN NaOH (48 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et20 (4 x 60 mL). The combined organic layer was washed with water and then brine, dried over Na2SO4, evaporated to give the title compound as white solids (1.83 g, 95%), which was used without further purification. MS (ESI) (M+H)+ = 229.
36F : 5-chloro-6-[2-[[(9-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohe pta[1,2-b]pyridin-5- yl) aminolcarbonyl] hydrazinol-N-f (3Rmethyl-2-oxo-3-pvrrolidinvl]- 3- pyridinecarboxamide.
To a solution of CDI (15.4 mg, 0.095 mmol) in dry DMF (1 mL) was added a solution of : 9-fluoro-10, 11-dihydro-5H-benzo [4,5] cyclohepta[1, 2-b] pyridin-5-amine (22 mg, 0. 095 mmol) in dry DMF (1 mL) at 0°C. After being stirred for 1 hour, the mixture was allowed to warm up to room temperature and 5-shloro-6-hydrazino-N-[(3R)-l-meiyl-2-oxo-3- pyrrolidinyl]-3-pyridinecarboxamide (27 mg, 0.095 mmol) was added. The mixture was stirred at room temperature for another 2 hours. The solvent was concentrated in vacuo, the residue was taken up into DCM (20 mL) and washed with water (1x10 mL), dried over Na2S04, concentrated and purified by flash chromatography (5-10% MeOH in DCM) to give the title compound as white solids (29 mg, 54%). 1H-NMR (400MHz, CD30D) : 8 8.62-8. 58 (m, 2H), 8.49-8. 52 (m, 1H), 8. 11 (d, J =2 Hz, 1H), 7.82 (t, J =8 Hz, 1H), 7. 30-7. 22 (m, 2H), 7.13-7. 04 (m, 1H), 6.53 (s, 1H), 4.70-4. 60 (m, 1H), 3.60-3. 35 (m, 6H), 2.55-2. 41 (m, 1H), 2.12-1. 98 (m, 1H) ppm. MS (ESI) (M+1)+=538. HRMS (M+1)+ Calcd for C26H2sClFN703 : 538.1769 ; Found: 538.1727. Anal. Calcd for C26H25CIFN703. 0. 2H20. 2.5TFA : C, 45.04 ; H, 3. 40 ; N, 11.86 ; Found: C, 45.08 ; H, 3.41 ; N, 11.80.
Example 37: 5-chloro-6- [2-[[(9-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohepta[1, 2- b] pyridin-5-yl) amino] carbonyl] hydrazino]-N- [ (3R)-tetrahydro-2-oxo-3-furanyl]- 3- pyridinecarboxamide. 37A: 5-chloro-6[2-[[(9-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohep ta[1,2-b]pyridin-5- yl) amino] carbonyljihydrazino7- 3-pvridinecarboxvlic acid.
To a solution of CDI (61 mg, 0.38 mmol) in dry DMF (4 mL) was added a solution of 9-fluoro-10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-b] pyridin-5-amine (87 mg, 0. 38 mmol) in dry DMF (4 mL) at 0°C. After being stirred for 1 hour, the mixture was allowed to warm up to room temperature and 5-chloro-6-hydrazino-3- pyridinecarboxylic acid (71 mg, 0.38 mmol) was added. The mixture was stirred at room temperature for another 2 hours. The solvent was concentrated in vacuo, the residue was treated with small amount of MeOH and diethyl ether, the solid was collected and dried to give the title compound (139 mg, 83%). MS (ESI) (M+H) += 442.
37B: 5-chloro-6-[2-[[(9-fluoro-10,11-dihydro-5H-benz9o[4,5]cycloh epta[1,2-b]pyridin-5- yl aminolcarbonyl] hrdrazinol-N-ff3R)-tetrahydro-2-oxo-3-furanvl]- 3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6-[2-[[(9-fluoro-10,11-dihydro-5H- benzo [4, 5] cyclohepta [1, 2-b] pyridin-5-yl) amino] carbonyl] hydrazino]- 3- pyridinecarboxylic acid (84 mg, 0.19 mmol) in DMA (2 mL) was added HATU (87 mg, 0.23 mmol) followed by DIPEA (39 p. L, 0.23 mmol). The mixture was stirred at room temperature for 10 min, and then (R)-(+)-a-amino--butyrolactone hydrochloride (32 mg, 0. 23 mmol) was added. The resulting mixture was stirred at room temperature for 4 h.
The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with concentrated NaHCO3 (2 x 10 mL) and then brine (1 x 10 mL).
The residue obtained after removal of the solvent was purified by reverse-phase HPLC (10-40% MeCN in water) to provide the title compound (37 mg, 31%) as a TFA salt. 1H-
NMR (400MHz, CD30D) : 8 8.59 (s, 1H), 8.57 (s, 1H), 8. 55-8. 51 (m, 1H), 8.10 (d, J = Hz, 1H), 7.82-7. 77 (m, 1H), 7.30-7. 20 (m, 2H), 7.13-7. 04 (m, 1H), 6.54 (s, 1H), 4.81-4. 75 (m, 1H), 4.52-4. 48 (m, 1H), 4. 39-4. 31 (m, 1H), 3. 62-3. 36 (m, 4H), 2.65-2. 56 (m, 1H), 2.46- 2.36 (m, 1H) ppm. MS (ESI) (MA-525. Calcd for C25H22ClFN6O4 : HRMS (M+H) + 525.1453 ; Found: 525.1307.
Example 38: 5-chloro-6-[2-[[(7-fluoro-110,11-dihydro-5H-benzo [4, 5] cyclohepta [1, 2- b pyridin-5-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-l-methyl-2-oxo-3-<BR> pyrrolidinyl]-3-pyridinecarboxamide.
38A : 24 (E)-2-(4-fluorophenyl)ethenyl]- 3-pyridinecarboxylic acid, sodium salt.
The mixture of NaH (1.32g, 60% in mineral oil, 55 mmol), t-BuOH (4.31 mL, 45 mmol) in DMF (30 mL) was stirred at room temperature for 10 minutes and then warm up to 50°C until the formation of air bubbles ceased. The mixture was cooled to 0°C and a solution of ethyl 4-methylnicotinate (2.5 g, 15 mmol) in DMF (5 mL) was added. After being stirred at the same temperature for 1 hour, 4-fluorobenzaldehyde (1.93 mL, 18 mmol) in DMF (5 mL) was added and the mixture was warmed up to room temperature and stirred for overnight. After being concentrated, the residue was used for the next step without further purification. MS (ESI) (M+H) + = 244.
38B: 2- [-4-fluorophenyl) ethvll-3-p, vridinecarboxylic acid, sodium salt.
The above sodium salt of 2-[(E)-2-(4-fluorophenyl) ethenyl3-3-pyridinecarboxylic acid was dissolved in MeOH (60 mL) and the mixture was hydrogenated at-40 psi for 3 h in the presence of 10% Pd/C at room temperature. After filtration, the filtrate was concentrated dried in vacuo and the solid was used for the next step without further purification. MS (ESI) (M+H) += 246.
38C : 7-fluoro-10, 11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin-5-one. F I polyphosphorous acid heat O/," O F N ONa 0 F To the above sodium salt of 2- [2- (2-fluorophenyl) ethyl]- 3-pyridinecarboxylic acid was added PPA (100 g) and the mixture was stirred at 160°C for 6 h. After cooling to 100°C, the mixture was poured into crushed ice (100 g) and the solution was carefully basified with KOH to pH >10. After extraction with EtOAc (4x 50 mL), dried over Na2S04 and evaporation, the title compound was obtained as pale yellow solids (2.1 g, 61% for 3 steps) and was used for the next step without further purification. MS (ESI) (M+H) + = 228.
38D : 7-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin -5-one, oxime,
2. 5 ex H2NOH. HCI \--,/ pyridin- S F 1000C N, F OH To a stirred solution of 7-fluoro-I0, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2- b] pyridin-5-one (1. 9 g, 8.36 mmol) in pyridine (10 mL) at 100°C was added H2NOH. HCl (1.45 g, 20.9 mmol) and the resulting mixture was stirred at the same temperature overnight. After the reaction was completed, pyridine was removed in vacuum, the residue was added water and the precipitate was collected by filtration, washed with water, dried to give the title compound as pale yellow powders (2.01 g, 99%), which was used without further purification. MS (ESI) (M+H) + = 243.
38B : 7-fluoro-10, 11-dihydro-5H-benzo[4,5]cyclohepta[1,2-b]pyridin-5-amin. 5 eq Zn powder \ 1 eq NH40Ac N F EtOH/DMF/28% NH40H | F OH reflux Un To a mixture of 7-fluoro-10, 11-dihydro-5H-benzo [4,5] cyclohepta [1, 2-b] pyridin- 5-one oxime (2.01 g, 8.31 mmol) in EtOH (18 mL), DMF (8 mL) and 28% NH40H (32 mL) was added Zn powder (2.71 g, 41.5 mmol) followed by NH40Ac (0.65 g, 8. 31 mmol). The mixture was stirred at reflux for 3 h. After cooled to room temperature, Et20 (120 mL) was added and stirred for 20 min, then IN NaOH (48 mL) was added and stirred for another 10 min. After filtered through Celite, the organic layer was isolated, and the basic aqueous solution was extracted with Et20 (4 x 60 mL). The combined organic layer was washed with water and then brine, dried over Na2S04, evaporated to give the title compound as white solids (1.84 g, 97%), which was used without further purification. MS (ESI) (M+H) + = 229.
38F : 7-fluoro-10* dihydro-5-isothiocyanato-5H-benzof4. 5lcacloheptarl *2-blparidine. CS2lEDC triethyl amine NH2 F THF NCS
To a solution of 7-fluoro-6, 11-dihydro-5H-benzot5, 6] cyclohepta [1, 2-c] pyridin- 11-amine (228 mg, 1.0 mmol) in dry THF (8 mL) was added CS2 (0.6 mL, 10 mmol) at- 10°C, then EDC (383 mg, 2.0 mmol) was added in one portion. After being stirred for 15 minutes, TEA (279 pLL, 2.0 mmol) was added dropwise. The mixture was allowed to slowly warm up to room temperature and stirred for 4 hours. After filtration, the filtrate was concentrated and the residue was taken up in ethyl acetate (30 mL), extracted with saturated NaHC03 (1x10 mL), water (1x10 mL) and then brine (1x10 mL), dried over Na2SO4, then concentrated in vacuo. The residue was purified by passing through a short pad of silica gel (30% EtOAc in DCM) to give the title compound as white solids (229 mg, 85%). MS (ESI) (M+H) + = 271.
3 8G : 5-chloro-6-[2-[[(7-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohe pta[1,2-b]pyridin-5- yl) amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-pyrro lidinyl]- 3- pyridinecarboxamide.
To a stirred solution of 7-fluoro-10, 11-dihydro-5-isothiocyanato-SH- benzo [4,5] cyclohepta [1, 2-b] pyridine (27 mg, 0.1 mmol) in DMA (2 mL) was added 5- chloro-6-hydrazino-N-[(3R)-1-methyl-2-oxo-3-pyrrolidinyl]-3- pyridinecarboxamide (28.4 mg, 1.0 mmol). The mixture was stirred at room temperature for 2 hours and then concentrated in vacuo, the residue was purified by flash chromatography (5-10% MeOH in DCM) to give the title compound as white solids (18 mg, yield 32%).'H-NMR (400MHz, CD30D): 8 8.62 (s, 1H), 8. 31 (d, J = 5Hz, 1H), 8.15 (d, J = 2Hz, 1H), 7.90 (d, J = 8Hz, 1H), 7.53 (s, br, 1H), 7.29-7. 20 (m, 2H), 7.13 (d, J = 8Hz, 1H), 6.96-6. 90 (m, 1H), 4. 70-4.61 (m, 1H), 3.48-3. 42 (m, 2H), 3.42-3. 05 (m, 4H), 2.89 (s, 3H), 2.53-2. 43 (m, 1H), 2.11-2. 00 (m, 1H) ppm. MS (M+H) + = 554. HRMS (M+1) + Calcd for C26H2sClFN7O2S : 554.1541 ; Found : 554.1533.
Example 39: 5-chloro-6-[2-[[(7-fluoro-10,11-dihydro-5H-benzo[4, 5] cycloheptal1, 2- b] pyridin-5-yl) amino] thioxomethyl] hydrazino]-N- [ (3R)-2-oxo-1-phenyl-3- pyrrolidinyl]-3-pyridinecarboxamide. 39A: 5-chloro-6-[2-[[(7-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohe pta[1,2-b]pyridin-5- yl)amino]thioxomethyl]hydrazino]- 3-pyridinecarboxylic acid.
To a solution of 7-fluoro-10, 11-dihydro-5-isothiocyanato-5H- benzo [4,5] cyclohepta [1, 2-b] pyridine (141 mg, 0.52 mmol) in DMA (3 mL) was added 5- chloro-6-hydrazino-3-pyridinecarboxylic acid (98 mg, 0.52 mmol), the mixture was stirred at room temperature for 2 hours, the solvent was concentrated in vacuo and water was added, the precipitate was collected by filtration, dried to give the title compound as off-white powders (213 mg, 90%). MS (ESI) (M+H) + = 458.
39B: 5-chloro-6-[2-[[(7-fluoro-10,11-dihydro-5H-benzo[4,5]cyclohe pta[1,2-b]pyridin-5- yl) thioxomethvllhvdrazino]-N- (3R)-2-oxo-l-phenyl-3-pyrrolidinyll- 3- pyridinecarboxamide.
To a stirred solution of 5-chloro-6- [2- (j (7-fluoro-10, 11-dihydro-SH- . benzo (4, 5] cyclohepta (1, 2-b] pyridin-5-yl) amino] thioxomethyl] hydrazino]- 3- pyridinecarboxylic acid (115 mg, 0.25 mmol) in DMA (4 mL) was added HATU (114 mg, 0. 3 mmol) followed by DIPEA (51 µL, 0.3 mmol). The mixture was stirred at room temperature for 10 min, and then (3R)-3-amino-1-phenyl-2-pyrrolidinone hydrochloride (64 mg, 0.3 mmol) was added. The resulting mixture was stirred at room temperature for 4 h. The product mixture was concentrated in vacuo, the residue was taken up in DCM (20 mL), and washed with conc. NaHC(O3 (2 x 10 mL) and then brine (1 x 10 mL). The residue obtained after removal of the solvent was purified by flash chromatography (0-5% MeOH in DCM) to provide the title compound (67 mg, 44%). 1H-NMR (400MHz, CD30D): # 8.64 (s, 1H), 8.25 (d, J = 5Hz, 1H), 8.10 (d, J = 2Hz, 1H), 7.81 (d, J = 5Hz, 1H), 7.63 (d, J = 8Hz, 2H), 7.49 (s, br, 1H), 7.37 (t, J = 8Hz, 2H), 7. 26-7.06 (m, 4H), 6.97- 6.83 (m, 1H), 4. 98-4. 82 (m, 1H), 4.01-3. 70 (m, 2H), 3. 46-3. 25 (m, 2H), 3. 24-3.01 (m, 2H), 2.65-2. 46 (m, 1H), 2.29-2. 11 (m, 1H) ppm. MS (ESI) (M+H) + =616. HRMS (M+1) + Calcd for C3lH27ClFN702S : 616.1698 ; Found: 616. 1641.
Example 40: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydr-5H-benzo[5,6]cyclohept a[1, 2- c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-N- (2-methoxy-3-pyridinyl)-3- pyridinecarboxamide.
40A : 5. 6-dichloro-N-(2-methoxy-3-pyridinyl)-3-pyridinecarboxamide
2 M Me3Al in heptane (0.5 ml, 1.0 mmol) was added dropwise to a solution of 2- methoxy-3-aminopyridine (124 mg, 1.0 mmol) in CH2C12 (5 ml) at 0°C. The mixture was stirred at room temperature for 30 min, then methyl 5, 6-dichloro-3-pyridinecarboxylate (204 mg, 1.00 mmol) from Experiment 15A was added. The mixture was stirred at room temperature for 3 h, quenched with H20 (lml), diluted with CH2Cl2 (30 ml), washed with 10% citric acid (5 ml), saturated NaHC03, brine, dried over Na2S04, concentrated in vacuo to give 5, 6-dichloro-N-(2-methoxy-3-pyridiyl)-3-pyridinecarboxamide (303 mg, quantitatively). MS (ESI) (M 298 (300).
40B: 5-chloro-6-hydrazino-N-(2-methoxy-3-pyridinyl)-3-pyridinecar boxamide 5, 6-dichloro-N- (2-methoxy-3-pyridinyl)- 3-pyridinecarboxamide (300 mg, lmmol) from step A and hydrazine monohydrate (0.1 ml) in EtOH (5 ml) was heated at reflux for 2 h, allowed to cool to room temperature, and the solid was collected (102 mg).
MS (ESI) (M+1) +-294.
40C: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohep ta[1,2-c]pyridin-11- yl)amino]thioxomethyl]hydrazino]-N-(2-methoxy-3-pyridinyl)-3 -pyridinecarboxamide
7-fluoro-6, 11-dihydro-11-isothiocyanato-5H-benzo [5,6] cyclohepta [1, 2-c] pyridine (27 mg, 0.10 mmol) from 4970X and 5-chloro-6-hydrazino-N- (2-methoxy-3-pyridinyl)- 3-pyridinecarboxamide (29 mg, 0.10 mmol) from 920B were dissolved in DMA (1 ml), and the reaction mixture was stirred at room temperature for 3 h, and H20 (10 ml) was added, and the solid was collected and dried. The crude product was further purified on silica gel (eluent MeOH : CH2Cl2 1: 25) to give 5-chloro-6-[2-[[(7-fluoro6,11-dihydro- 5H-benzo [5,6] cyclohepta [1, 2-c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-N- (2- methoxy-3-pyridinyl)-3-pyridinecarboxamide (47 mg, 83%).'HNMR (40OMHz, CDCI,) : 8 8. 58-8. 62 (m, 3H), 8.33 (s, 1H), 8.32 (s, 1H), 8. 11 (s, 1H), 8.00-8. 10 (br, 1H), 7.91 (d, J=1.7 Hz, 1H), 7.89 (d, J=1. 7 Hz, 1H), 7.21 (d, J=7.6 Hz, 1H), 7.05-7. 15 (m, 2H), 7.01 (d, J=6. 1Hz, 1H), 6.88-6. 98 (m, 2H), 4.05 (s, 3H), 3.14 (m, 4H) ppm. MS (ESI) (M+1) += 564.
HRMS (M+1) + Calcd for C21H23ClFN702S : 564.1385 ; Found: 564.1302.
Example 41 : 6- [2- [ [ (7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta [1, 2-c] pyridin- 11-yl) amino]thioxomethyl]hydrazino]-N-[(3R)-1-methyl-2-oxo-3-pyrro lidinyl]- 3- pyridinecarboxamide.
7-fluoro-6, 11-dihydro-11-isothiocyanato-SH-benzo [5,6] cyclohepta [ 1, 2-c] pyridine (27 mg, 0.10 mmol) and 6-hydrazinonicotinic acid (15 mg, 0.10 mmol) prepared from hydrazine and nicotinic acid were dissolved in DMA (2 ml), the reaction mixture was stirred at room temperature for 16 h, and the acid intermediate as its DMA solution was prepared. MS (ESI, M+1) + : 423.
(3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride (30 mg), DIPEA (0. 1 ml), HATU (65 mg) was added successively to the above acid intermediate. The mixture was stirred at room temperature for 2h, the reaction mixture was subject to preparative LCMS without work-up and the desired product (10 mg) was obtained as TFA salt.
IHNMR (400MHz, CD30D) : 8 8.81 (m, 1H), 8.64 (s, 1H), 8.59 (d, J-5. 9 Hz, 1H), 8.07 (m, 1H), 7.85 (d, J=3.9 Hz, 1H), 7.37 (br, 1H), 7. 22 (m, 2H), 7.04-7. 10 (m, 1H), 6.77 (d, J=8.8 Hz, 1H), 4.67 (m, 1H), 3.40-3. 50 (m, 4H), 3.33 (m, 1H), 3.15 (m, 1H), 2.90 (s, 3H), 2.40-2. 50 (m, 1H), 2.00-2. 10 (m, 1H) ppm. MS (ESI) (M+1) += 520. HRMS (M+1) + Calcd for C26H26FN702S : 520. 1931 ; Found: 520. 1981.
Example 42: 5-chloro-6-[[[[ (7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta [1, 2- <BR> <BR> <BR> <BR> c] pyridin-11-yl) amino] thioxomethyl] aminolmethyll-N-1 (3R)-tetrahydro-t oxo-3-<BR> <BR> <BR> <BR> <BR> <BR> <BR> thienyl]-3-pyridinecarboxamide.
42A: 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl) aminolthioxomethvllaminolmethylL 3-pvridinecarboxylic acid, methyl ester 7-fluoro-6, 11-dihydro-11-isothiocyanato-5H-benzo [5, 6] cyclohepta [1, 2-c] pyridine (270 mg, 1. 0 mmol) and methyl 6- (aminomethyl)-5-chloro-3-pyridinecarboxylate (220 mg, #1. 10 mmol) from Example 15C was dissolved in DMA (6 ml), the reaction mixture was stirred at room temperature overnight, and DMA was stripped off in vacuo. The residue was subjected to flash chromatography on silica gel (EtOAc: CH2CI2 1: 3) to give the desired thiourea compound (220 mg).
42B: 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl)amino]thioxomethyl]amino]methyl]- 3-pyridinecarboxylic acid.
1 N NaOH (1 ml, 1.0 mmol) was added to a solution of 5-chloro-6-[[[[(7-fluoro- 6, 11-dihydro-SH-benzo [5,6] cyclohepta [1, 2-cpyridin-11- yl) amino] thioxomethyl] amino] methyl]-3-pyridinecarboxylic acid methyl ester (220 mg,
0. 468 mmol) in a solvent mixture of MeOH/THF/H2O (7.5 ml, 1: 1: 1), the reaction mixture was heated at 50 °C for 30 min, then concentrated in vacuo, and then re-dissolved H20 (10 ml). 1N HC1 (1 ml) was added, the precipitate was collected and dried to afford the acid intermediate (210 mg), MS (M+1)+=457.
42C: 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl) aminolthioxomethyl] amino] methyll-N-f (3R)-tetrahydro-2-oxo-3-thienyl]-3- pyridinecarboxamide.
Following general HATU coupling procedure of 1C : HATU (42 mg, 0.11 mmol) . was added to a mixture of 5-chloro-6- [[(7-fluoro-6, 11-dihydro-SH- benzo [5,6] cyclohepta- [1, 2-c] pyridin-11-yl) amino] thioxomethyl] amino] methyl]- 3- pyridinecarboxylic acid (46 mg, 0.10 mmol) from step B, (D)-homocysteine thiolactone hydrochloride (22 mg), DIPEA (0.1 ml) in DMA (3 ml) at room temperature. The reaction mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure. H20 (10 ml) was added, and the precipitate was collected and dried. The product was purified on silica gel (EtOAc : CH2Cl21 : 3 to 5% MeOH in CH2Cl2) to give the title compound (48 mg, 86%). 1HNMR (400MHz, CD30D): 8 8.87 (br. s, 1H), 8.64 (s, 1H), 8.34 (d, J=5.1 Hz, 1H), 8. 23 (d, J = 1.9 Hz, 1H), 7.33 (d, J = 5.1 Hz, 1H), 7.31 (d, J = 7.6 Hz, 1H), 7.19-7. 26 (m, 1H), 7. 10 (br. s, 1H), 7. 00-7. 06 (m, 1H), 4.97 (s, 1H), 4.84-4. 90 (m, 2H), 3.40-3. 50 (m, 1H), 3.10-3. 35 (m, 4H), 2.60-2. 67 (m, 1H), 2.23-2. 33 (m, 1H) ppm. MS (ESI) (M+1) + : 556. HRMS (M+1) + Calcd for C26 H23 Cl F Ns 02 S2 : 556. 1044; Found: 556.1091.
Example 43: 5-chloro-6- [[[[(7-fluoro-6,11-dihydro-5H-benzo [5,6] cycloheptafl, 2- c] pyridin-11-yl) amino] thioxomethyl] amino] methyl]-N-[(3R)-1-methyl-2-oxo-3- pyrrolidinyl]- 3-pyridinecarboxamide.
Following general HATU coupling procedure of Example 1C : HATU (95 mg, 0.25 mmol) was added to a mixture of 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H- benzo [5,6] cyclohepta-[l, 2-c] pyridin-11-yl) aminogthioxomethyl] amino] methyl]-3- pyridinecarboxylic acid (92 mg, 0.20 mmol), (3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride (60 mg), DIPEA (0.2 ml) in DMA (5 ml) at room temperature. The reaction mixture was stirred at room temperature for 2 h, and then DMA was removed under reduced pressure. H20 (5 ml) was added, and the precipitate was collected and dried. The product was purified by preparative HPLC and lypholized to give the title compound (25 mg) as its TFA salt.'HNMR (CD30D) : 5 8.97 (br. s, 1H), 8.85 (s, 1H), 8.59 (d, J=5. 5 Hz, 1H), 8.23 (m, 1H), 7.89 (d, J = 5.9 Hz, 1H), 7.21-7. 44 (m, 3H), 7.05- 7.20 (m, 1H), 6.97 (s, 1H), 4.93 (s, 1H), 4.68 (m, 1H), 3.78 (m, 1H), 3.44 (m, 4H), 3.12 (m, 1H), 2.89 (s, 3H), 2.40-2. 55 (m, 1H), 2.00-2. 10 (m, 1H) ppm. MS (ESI) (M+l)"= 553. HRMS (M+1) + Calcd for C27H26ClFN6O2S : 553.1589 ; Found: 553.1575. CHN Calcd for C27 H26C1FN602S. 2CF3CO2H. 0. 5H2O : C, 47.12 ; H, 3.70 ; N, 10.64. Found: C, 46.70 ; H, 3.63 ; N, 11.14.
Example 44: 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cyclohepta [1, 2- c] pyridin-ll-yl) amino] carbonyl] amino] methyl]-N- [ (3R)-l-methyl-2-oxo-3- pyrrolidinyl]-3-pyridinecarboxamide.
44A: 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benz9o[5,6]cyclohep ta[1,2-c]pyridin-11- yl)amino]carbonyl]amino]methyl]-3-pyridinecarboxylic acid, methyl ester.
7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta [1, 2-c] pyridin-11-amine (120 mg, 0.52 mmol) was added to a solution of CDI (90 mg, 0.55 mmol) in DMF (5 ml) at room temperature and the reaction mixture was stirred at room temperature for 3 h. And then methyl 6- (aminomethyl)-5-chloro-3-pyridinecarboxylate (120 mg, mmol) was added and the reaction mixture was heated at 70°C for 3 h, DMF was evaporated in vacuo, and the residue was dissolved in CH2CI2 (30 ml), washed with H20 (2x5 ml), dried, and concentrated in vacuo. The product was purified on silica gel (5% MeOH in CH2C12) to give the title compound (150 mg, 63%). MS (ESI) (M+1)+= 455.
44B 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- ylaminolcarbonyl] aminoLmethyl]- 3-pyridinecarboxylic acid.
1 N NaOH (0. 8 ml, 0.8 mmol) was added to a solution of 5-chloro-6-[[[[(7-fluoro-
6, 11-dihydro-5H-benzo[5,6]cyclohepta[1, 2-c] pyridin-l 1- yl) amino] carbonyl] amino] methyl]- 3-pyridinecarboxylic acid methyl ester (150 mg, 0.330 mmol) from 928A in a solvent mixture of MeOH/THF/H20 (7. 5 ml, 1 : 1: 1), the reaction mixture was heated at 50 °C for 30 min, then concentrated in vacuo, and then re- dissolved H20 (5 ml). 1N HC1 (0.8 ml) was added, the precipitate was collected and dried to afford the acid intermediate (120 mg), MS (M+1) +=445.
44C : 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohept a[1,2-c]pyridin-11- yl)amino]carbonyl]amino]methyl]-N-[(3R)-1-methyl-2-oxo-3-pyr rolidinyl]-3- pyridinecarboxamide.
Following the general HATU coupling procedure in Example 1C : The title compound (42 mg, 52%) was obtained from 5-chloro-6-[[[[(7-fluoro-6,11-dihydro-5H- benzo [5,6] cyclohepta [1, 2-c] pyridin-11-yl) amino] carbonyl] amino] methyl]- 3- pyridinecarboxylic acid and (3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride.
'HNMR (400MHz, CD30D) : # 8.87 (s, 1H), 8.70-8. 80 (m, 2H), 8.52 (br, 1H), 8.22 (s, 1H), 7.70 (br, 1H), 7.20-7. 35 (m, 2H), 7. 08 (dd, J = 7.6, 8.0 Hz, 1H), 6.31 (s, 1H), 4.68 (m, 1H), 4.59 (s, 2H), 3.67 (m, 1H), 3.30-3. 50 (m, 4H), 3.10-3. 20 (m, 1H), 2. 89 (s, 3H), 2.45-2. 55 (m, 1H), 1.95-2. 10 (m, 1H) ppm. MS (ESI) (M+1) +=537. HRMS (M Calcd for C27H26ClFN603 : 537.1817 ; Found: 537. 1875.
Example 45: [5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo [5,6] cyclohepta [1, 2- c] pyridin-11-yl) amino] thioXomethyl] hydrazino]-3-pyridinyl] methyl-carbamic acid, methyl ester.
45A: (5, 6-dichloro-3-pvridinvl)-carbamic acid 1s1-dimethylethyl ester. H Cl t NHz goc O C I N1/O I' z Cl4 zozo I-0 Cl dioxane Cl Boc2O (3.50 g, 16.0 mmol) was added to a solution of 5, 6-dichloro-3- pyridinamine (2.00 g, 12.34 mmol) in dioxane, and the mixture was heated at reflux for 8 h, allowed to cool to room temperature. The solvent was stripped off at the reduced pressure, and the residue was dissolved in CH2Cl2 (50 ml) and washed with saturated NaHC03, brine, dried over Na2S04, concentrated in vacuo. The product was purified on silica gel (CH2CI2 : EtOAc 1: 2) to give the title compound (1.05 g), MS (ESI) (M+1) +=263.
45B : 5,6-dichloro-N-methyl-3-pyridinamine.
60% NaH (240 mg, 6.0 mmol) was added to the solution of (5,6-dichloro-3- pyridinyl) -carbamic acid 1, 1-dimethylethyl ester (1.00 g, 3.80 mmol) in THF (30 ml) at 0°C, and the reaction mixture was stirred at this temperature for 30 min, then MeI (0. 40 ml, 6.4 mmol) was added, and the mixture was stirred at room temperature for 2 h. THF was distilled in vacuo and the residue was dissolved in CH2Cl2, and washed with brine, dried over Na2S04. Removal of solvent gave the crude N-Me product that was used without purification, MS (ESI) (M+1) +=277. The N-Methyl compound was dissolved in EtOAc (30 ml), and HCl (g) was bubbled into the solution for 10 min, and the mixture was stirred at room temperature for 2 h and EtOAc was evaporated in vacuo to give the title compound (640 mg).
45C: (5-chloro-6-hvdrazino-3-pyridinyl) methvl- carbamic acid, methyl ester. H Me I el 1 0111. cl N-, CICO o NH, NH,-."Y I",--0 1-*"0 N DIPEA c microwave HN, NT NH2
Methyl chlorotormate (40 mg, 0. 42 mmol) was added to a mixture of 5,6- dichloro-N-methyl-3-pyridinamine (36 mg, 0.20 mmol) and DIPEA (0.15 ml) in CH2C12 (1 ml) at 0°C. The reaction mixture was stirred at room temperature overnight, diluted with CH2Cl2 10 ml), washed with NaHCO3, brine and dried. Removal of solvent gave the methyl carbamate intermediate. 1 M NH2NH2 solution in pentanol (2 ml) was added to the intermediate, and the reaction mixture was heated in a Smith station microwave instrument at 180°C for 10 min. Removal of solvent and excess hydrazine afforded the title compound which was used without purification. MS (ESI) (M+1) +=231.
45D : [5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohe pta[1,2-c]pyridin-11- yl) aminolthioxomethyllhvdrazinol-3-pvridinYllmethyl-carbamic acid, methyl ester.
The mixture of 7-fluoro-6, 11-dihydro-11-isothiocyanato-5H- benzo [5,6] cyclohepta [1, 2-c] pyridine (54 mg, 0. 20 mmol) and methyl (5-chloro-6- hydrazino-3-pyridinyl) methyl-carbamate (-0. 2 mmol) from 925C in DMF (2 ml), and the mixture was stirred at room temperature overnight and purified on prep-LCMS to give the title compound (25 mg) as its TFA salt.'HNMR (CD30D) : 8 8. 83 (d, J= 4.1 Hz, 1H), 8.45-8. 53 (m, 1H), 7.76 (dd, J=6.0, 7.3 Hz, 1H), 6. 90-7. 40 (m, 6H), 3.19 (s, 3H), 3.15 (s, 3H), 2. 90-3. 60 (m, 4H) ppm. MS (ESI) (M+1) += 501. HRMS (M+1) + Calcd for C23H22CIFN602S : 501.1276. Found: 501. 1211. Example 46: N-l5-chloro-6-l2-lL (7-fluoro-6, 11-dihydro-5H-benzo [5, 6] cycloheptal1, 2- c] pyridin-11-yl) amino] thioxomethyl] hydrazino]-3-pyridinyl]-N-methyl- cyclopropanecarboxamide.
46A: N-(5-chloro-6-hydrazino-3-pyridinyl)-N-methyl-cyclopropaneca rboxamide.
Following the procedure of Example 45C: cyclopropanecarbonyl chloride (40 mg, 0.40 mmol) was added to a mixture of 5, 6-dichloro-N-methyl-3-pyridinamine (36 mg, 0.20 mmol) and DIPEA (0.15 ml) in CH2C12 (1 ml) at 0°C. The reaction mixture was stirred at room temperature overnight, diluted with CH2Cl2 10 ml), washed with NaHCO3, brine and dried. Removal of solvent gave the amide intermediate, MS (ESI) (M+1) +=245. 1 M NH2NH2 solution in pentanol (2 ml) was added to the intermediate, and the reaction mixture was heated in a Smith station microwave at 180°C for 10 min. Removal of solvent and excess hydrazine afforded the title compound that was used without purification. MS (ESI) (M+1) +=241.
46B : N-[5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclo hepta[1,2-c]pyridin- 1 1-yl) aminolthioxomethvllhvdrazino]-3-pyridinvll-N-methyl-cyclopro panecarboxamide.
Following the procedure Example 45D: The mixture of 7-fluoro-6, 11-dihydro-11- isothiocyanato-5H-benzo [5,6] cyclohepta [1, 2-c] pyridine (54 mg, 0.20 mmol) and N- (5- chloro-6-hydrazino-3-pyridinyl)-N-methyl-cyclopropanecarboxa mide (-0. 2 mmol) from 923A in DMF (2 ml), and the mixture was stirred at room temperature overnight and purified on prep-LCMS to give the title compound (26 mg) as its TFA salt. 1HNMR (400MHz, CD30D): 8 8.75 (s, 1H), 8.40-8. 52 (m, 2H), 7.98 (s, 1H), 7.60-7. 75 (m, 2H), 6.90-7. 25 (m, 4H), 3.14 (s, 3H), 3.00-3. 50 (m, 4H), 0.50-1. 25 (m, 5H) ppm.
(MS (ESI) (M+1) +=511. HRMS (M+l) +Calcd for C25H24CIFN60S : 511.1483. Found: 511.1412.
Example 47: N- [5-chloro-6-12-1 [ (7-fluoro-6, 11-dihydro-5H-benzo [5, 6] cyclohepta[1, 2- clpyridin-ll-yl) amino] thioxomethyl] hydrazino]-3-pyridinyl]-N-methyl-3- pyridinecarboxamide.
47A: N-(5-chloro-6-hydrazino-3-pyridinyl)-N-methyl-3-pyridinecarb oxamide. N - zizi N I I i O H PEA microwave Cl DIPEA Cul N cri N NH2 Following the procedure of Example 45C: nicotinoyl chloride hydrochloride (71
mg, 0.40 mmol) was added to a mixture of 5, 6-dichloro-N-methyl-3-pyridinamine (36 mg, 0.20 mmol) and DIPEA (0.25 ml) in CH2CI2 (1 ml) at 0°C. The reaction mixture was stirred at room temperature overnight, diluted with CH2CI2 10 ml), washed with NaHCO brine and dried. Removal of solvent gave the amide intermediate, MS (ESI) (M+1) +=282. 1 M NH2NH2 solution in pentanol (2 ml) was added to the intermediate, and the reaction mixture was heated in a Smith station microwave at 180°C for 10 min. Removal of solvent and excess hydrazine afforded the title compound that was used without purification. MS (ESI) (M+1)+=278. <BR> <BR> <BR> <BR> <BR> <P>47B : N- 5-chloro-6-r2- [ [7-fluoro-6. 11-dihvdro-5H-benzof5, 61cvclohepta [l, 2-clpyridin- 11-yl)amino]thioxomethyl]hydrazino]-3-pyridinyl]-N-methyl-3- pyridinecarboxamide.
Following the procedure of Example 45D: The mixture of 7-fluoro-6, 11-dihydro- 11-isothiocyanato-5H-benzo [5, 6] cyclohepta [1, 2-c] pyridine (54 mg, 0.20 mmol) andN- [5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohe pta[1,2-c]pyridin-11- yl) arnino] thioxomethyl] hydrazino]-3-pyridinyl]-N-methyl-3-pyridinecarboxamide (-0. 2 mmol) from step 47A in DMF (2 ml), and the mixture was stirred at room temperature overnight and purified on prep-LCMS to give the title compound (27mg).
'HNMR (400MHz, CD30D) : # 8.78 (s, 1H), 8.62 (d, J = 5. 8 Hz, 1H), 8.47 (br, 1H), 7.80- 8.00 (m, 4H), 7. 00-7.50 (m, 7H), 3.43 (s, 3H), 3.25-3. 50 (m, 3H), 3.05-3. 20 (m, 1H) ppm.
MS (ESI) (M+1)+=548. HRMS (M+1) + Calcd for C27H23CIFN70S : 548.1435 ; Found: 548. 1475.
Example 48: 2-[3-chloro-5-[[[(3R)-1-methyl-2-oxo-3-pyrrolidinyl]amino]su lfonyl]-2- pyridinyl]-N-(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cyclohepta [1,2-c]pyridin-11-yl)- hydrazinecarbothioamide.
48A : 5, 6-dichloro-N- [3R)-1-methvl-2-oxo-3-pvrrolidinvl]- 3-pyridinesulfonamide.
A solution of 5, 6-dichloro-3-pyridinesulfonyl chloride (Prepared according to: M. W. Crawley, European Patent Application No. EP0147105A2 (1985) ) (34 mg, 0.14 mmol) in CH2Cl2 (3 mL) was added to a mixture of (3R)-3-amino-1-methyl-2- pyrrolidinone hydrochloride (60 mg, 0.40 mmol) and DIPEA (0.20 mL, ) in CH2C12 (3 ml) at-30°C, the reaction mixture was stirred at this temperature for 30 min, allowed to warm to room temperature, diluted with EtOAc (20 mL), washed with 5% HCl, H2O, and brine, dried over Na2SO4. Removal of solvent gave the title compound (15 mg), MS (ESI) (milz + : 324.
48B: 5-chloro-6-hydrazino-N-[(3R)-1-methyl-2-oxo-3-pyrrolidinyl]- 3- pyridinesulfonamide. O O O'H N, O H/ CI S\o NHzNN2 Ci S N _ EtOH, microwave ce HN N I NH2 A solution of 5, 6-dichloro-N- [ (3R)-1-methyl-2-oxo-3-pyrrolidinyl]- 3- pyridinesulfonamide (15 mg, 0.046 mmol), and 1M NH2NH2 in pentanol (0.30 ml, 0.30 mmol) in EtOH (0. 50 ml) was heated in microwave for 10 min, allowed to cool to room temperature. The solid was collected and the mother liquid was concentrated in vacuo and
lipholized. The product was combined to give the title compound (12 mg, 80%). MS (ESI) (M+l) + = 320.
48C: 2-[3-chloro-5-[[[(3R)-1-methyl-2-oxo-3-pyrrolidinyl]amino]su lfonyl]-2-pyridinyl]- N- (7-fluoro-6, 11-dihvdro-5H-benzo [5, 6]'cycloheptaf 1, 2-clpvridin-11-yl)- hydrazinecarbothioamide.
5-chloro-6-hydrazino-N-[(3R)-1-methyl-2-oxo-3-pyrrolidinyll- 3- pyridinesulfonamide (6.4 mg, 0.02 mmol) and 7-fluoro-6, 11-dihydro-11-isothiocyanato- 5H-benzo [5,6] cyclohepta [1, 2-c] pyridine (5.4 mg, 0.02 mmol) was dissolved in DMA (1 ml) at room temperature and the reaction mixture was stirred at room temperature overnight. DMA was stripped off and the residue was lipholized to afford the title compound (12 mg, quantitatively).'HNMR (CD30D) : 8 8.55 (s, 1H), 8.51 (d, J = 2 Hz, 1H), 8.33 (m, 2H), 8.08 (m, 1H), 6. 90-7. 55 (m, 6H), 4.06-4. 16 (m, 1H),, 3.00-3. 50 (m, 6H), 2.68 (s, 1. 5H), 2.65 (s, 1. 5H) (2.65 and 2. 68 peaks from the N-Me of the two diastereoisomers), 2.32-2. 44 (m, 1H), 1.75-1. 90 (m, 1H) ppm. MS (ESI) (M+1)+=590.
HRMS (M+1) +Calcd for C25H25ClFN7O3S2 : 590. 1211 ; Found: 590.1141.
Example 49: 5-chloro-6- [2- [ [ (7-fluoro-6, 11-dihydro-5H-benzo [5,6] cyclohepta [1, 2- c] pyridin-11-yl) amino]thioxomethyl]-1-methylhydrazino]-N-[(3R)-1-methyl-2-ox o-3- pyrrolidinyl]-3-pyridinecarboxamide.
49A : 5-chloro-6- (l-methylhydrazino 3-pvridinecarboxylic acid.
The mixture of 5, 6-dichloronicotic acid (2.00 g, 10. 42 mmol) and methylhydrazine (2. 50 ml) in EtOH (15 ml) was heated at reflux for 3 days, then EtOH was striped off, and then H20 (10 ml) was added, and the solution was neutralized with HOAc to pH#7. The precipitate was collected and dried to give the title compound (1.80 g, 86%).
49B: 5-chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5,6]cyclohep ta[1,2-c]pyridin-11- yl) aininolthioxomethyll- 1-methylhydrazinol-N-r (3R) pyridinecarboxamide.
7-fluoro-6, 11-dihydro-11-isothiocyanato-SH-benzo [5, 6] cyclohepta [1, 2-c] pyridine (41mg, 0.15 mmol) and 5-chloro-6-(1-methylhydrazino)- 3-pyridinecarboxylic acid (30 mg, 0. 15 mmol) were dissolved in DMA (3 ml), the reaction mixture was stirred at room temperature for 16 h, and the acid intermediate as its DMA solution was prepared.
MS (ESI, M+1)+: 427.
(3R)-3-amino-1-methyl-2-pyrrolidinone hydrochloride (45 mg), DIPEA (0.15 ml), HATU (65 mg) was added successively to the above acid intermediate. The mixture was stirred at room temperature for 2h, DMA was stripped off in vacuo, and the residue was treated with H20 (5 ml), the solid was collected and washed with H20 (2x5 ml), dried in vacuo to give the title compound (53 mg).'HNMR (CD30D) : 8 8. 75 (s, 1H), 8.62 (d, J = 3.9 Hz, 1H), 8. 55 (d, J = 4.3 Hz, 1H), 8.28-8. 40 (m, 2H), 8.03 (s, 1H), 7.48 (m, 1H), 6.90- 7. 40 (m, SH), 4.56 (dd, J = 9.0, 9.1 Hz, 1H), 3.00-3. 50 (m, 6H), 3.10 (s, 3H), 2.79 (s, 3H), 3. 30-2.45 (m, 1H), 1.85-2. 05 (m, 1H) ppm. MS (M+1)+=568. HRMS (M+1) + Calcd for C27H27C1FN702S : 568. 1698; Found : 568.1772.
Example 50: 5-Chloro-6-[2-[[(7-fluoro-6,11-dihydro-5H-benzo[5, 6] cycloheptall, 2- <BR> <BR> <BR> c] pyridin-11-yl) amino] carbonyl] hydrazino]-N- [ (3S)-1-methyl-5-oxo-3-pyrrolidinyl]- 3-pyridinecarboxamide.
50A : 2-[[(1,1-Dimehtylethoxy)carbonyl]amino]-3-(methylamino)-3-ox o-phenylmethyl ester, (2S !-propanoic acid.
HATU (7.05 g, 18.55 mmol) was added to a mixture of Boc-L-Asp (OBz) -OH (5 g, 15.46 mmol), CH3NH2. HCl (3.3 g, 48.87 mmol) and DIPEA (13.42 mL, 77.07 mmol) in DMA (20 ml). The mixture was stirred at room temperature overnight and then DMA was removed under reduced pressure. The residue was then diluted in CH2C12 and washed with saturatedNaHCO3, water and brine, dried and concentrated. The product was then purified by column chromatography (CH2Cl2-MeOH) to afford the title product (2.10 g, 40%). 1H NMR (400 MHz, CDCl3) : 8 7.41-7. 30 (m, 5H), 6.41 (bs, 1H), 5.64 (bs, 1H), 5.17-5. 07 (m, 2H), 4.84 (s, 1H), 3.11-3. 02 (m, 1H), 2.77 (d, J = 4.9 Hz, 3H), 2.69 (dd, J = 17.3 and 6.1 Hz, 1H), 1. 43 (s, 9H) ppm. MS (ESI) (M+1) + : 337.
50B : N- [ (1, 1-Dimethylethox) carbonvl-3- (methvlamino)-3-thioxo-phenylmethyl ester- L-alanine.
A mixture of 0. 2 g (0. 59 mmols, 1 eq) of 2-[[(1,1- dimethylethoxy) carbonyl] amino]-3- (methylamino)-3-oxo-phenylmethyl ester, (2S)- propanoic acid and phosphorus pentasulfide (0. 41 g, 0.92 mmols, 1.6 eq. ) in THF (5 mL) was heated to 60 °C for 1 hour. The mixture was then cooled to the room temperature and AcOEt was added. The organic phase was then washed with saturated NaHC03, water and brine, dried and concentrated. The product was directly used for the next step. MS (ESI) (M+1) + : 353.
50C: [ (3S)-1-Methyl-5-oxo-3-p. vrrolidinyll-carbamic acid, 1, 1-dimethylethyl ester.
NiC12. 6H20 (0. 21 g, 5.6 mmol) and N-[(1,1-dimethylethoxy)carbonyl]-3- (methylamino)-3-thioxo-phenylmethyl ester-L-alanine (85 mg, 0.24 mmol) were dissolved in EtOH/THF (1/1 mL) and the mixture was cooled at 0 °C. NaBH4 (0. 1 lg, 2.9 mmol) was then added and the reaction was stirred 30 minutes. The mixture was then filtered over celite and the filtrate evaporated under reduced pressure. The residue was dissolved in CH2Cl2 and was washed with 5% NaHCO3. The organic layer was separated and washed with water and brine, dried and concentrated under reduced pressure. The crude product was then purified by column chromatography (CH2Clz-MeOH). 1H NMR (400 MHz, CDC13) : 8 4.93 (bs, 1H), 4.26 (bs, 1H), 3.71-3. 62 (m, 1H), 3.21 (dd, J = 10. 5 and 3.8 Hz, 1H), 2.82 (s, 3H), 2 : 71 (dd, J = 17. 3 and 8. 3 Hz, 1H), 2.22 (dd, J = 17.0 and 4.5 Hz, 1H), 1.42 (s, 9H) ppm. MS (ESI) (M+1) + : 215.
50D : 4-Amino-1-methvl-monohydrochloride, (4S)-vrrolidinone.
A solution of [ (3S)-1-Methyl-5-oxo-3-pyrrolidinyl]-1, 1-dimethylethyl ester-carbamic acid (0. 14 g, 0. 65 mmols) in AcOEt (3 mL) was treated with HCl (gas) at 0 °C for 1 hour. The stirring was then continued for 3 hours. The solvent was removed under reduced pressure and the crude product was directly used for the next step.
50E : 5. 6-Dichloro-N-f (3 S)-1-methyl-5-oxo-3-pvrrolidinyl-3-pyridinecarboxamide.
HATU (0. 46 g, 1.21 mmols, 1.2 eq. ) was added to a mixture of 5,6- dichloronicotinic acid (0.23 g, 1.20 mmols, 1 eq. ), 4-amino-l-methyl- monohydrochloride, (4S) -2-pyrrolidinone (0. 3 g, 1. 99 mmols, 1 eq. ) and DIPEA (0.8 mL, 4.59 mmols, 2.3 eq.) in 10 mL DMA. The mixture was stirred at rt overnight and then DMA was removed under reduced pressure. The residue was then diluted in CH2CI2 and washed with saturated NaHC03, water and brine, dried and concentrated. The crude product was then purified by column chromatography (CH2Cl2-MeOH) to afford the title compound (0.14 g, 40%). MS (ESI) (M+1) + : 289.
50F : 5-Cloro-6-hydrazino-N-[(3S)-1-methyl-5-oxo-3-pyrrolidinyl]- 3- p Yridinecarboxamide.
A solution of 5, 6-dichloro-N- [ (3S)-l-methyl-2-oxo-3-pyrrolidinyl]-3- pyridinecarboxamide (80 mg, 0.28 mmol) in EtOH (1.5 mL) was treated with hydrazine. monohydrate (60 L, 4.4 eq. ) and the mixture was refluxed for 3 hours. The reaction was then cooled to room temperature and the solid was filtered and washed with EtOH to provide the title compound (76 mg, 96 %).
50G : 5-Chloro-6-r2- [ [ (7-fluoro-6, 11-dihvdro-5H-benzor5, 61cycloheptarl, 2-clP32idin-11- yl)aminocarbonyl]hydrazino]-N-[(3S)-1-methyl-5-oxo-3-pyrroli dinyl]-3- pyridinecarboxamide.
To a solution of 7-fluoro-6, 11-dihydro-11-isothiocyanato-SH- benzo [5,6] cyclohepta [1, 2-c] pyridine (54 mg, 0.2 mmols, 1 eq) in 3 mL DMF was added 5-chloro-6-hydrazino-N- [ (3S)-1-methyl-5-oxo-3-pyrrolidinyl]-3-pyridinecarboxamide (76 mg, 0.2 mmols, 1 eq) and the mixture was stired overnight. The solvent was then removed under reduced pressure and the residue was then diluted in CHzCl2 and washed with a saturated NaHC03, water and brine, dried and concentrated under reduced pressure. The product was then purified by column chromatography (CH2Cl2-MeOH) to afford the title product (22 mg, 27%). 1H NMR (400 MHz, CDC13) : 8 7.27 (bs, 1H), 7.14 (bs, 1H), 7.05 (d, J = 6.0 Hz, 1H), 6.59 (s, 1H), 6.34 (d, J = 6.0 Hz, 1H), 5.99 (s, 1H), 5.75-5. 63 (m, 2H), 5.50 (t, J = 8.5 Hz, 1H), 3.15-3. 03 (m, 1H), 2.27 (dd, J = 10.5 and 7.4 Hz, 1H), 2.02-1. 84 (m, 3H), 1.79-1. 60 (m, 2H), 1.30 (s, 3H), 1.30-1. 18 (m, 2H), 0.96 (dd, J = 17.4 and 4.5 Hz, 1H) ppm. MS (ESI) (M 555.
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