Title:
THERMOSTABLE B-FAMILY DNA POLYMERASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/247055
Kind Code:
A1
Abstract:
Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant. Compared with non-mutant DNA polymerases, several groups of mutant DNA polymerases exhibit a strong polymerization capability in catalyzing 3' blocking-modified nucleotides and provide a basis for the sustainable development of high-throughput sequencing.
Inventors:
ZHAI LILI (CN)
XIE QINGQING (CN)
CAO RUYIN (CN)
ZHENG YUE (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU XUN (CN)
CHEN ZHUO (CN)
XIE QINGQING (CN)
CAO RUYIN (CN)
ZHENG YUE (CN)
DONG YULIANG (CN)
ZHANG WENWEI (CN)
XU XUN (CN)
CHEN ZHUO (CN)
Application Number:
PCT/CN2021/117113
Publication Date:
December 01, 2022
Filing Date:
September 08, 2021
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12N9/12; C12Q1/68
Domestic Patent References:
| WO2020048329A1 | 2020-03-12 | |||
| WO2018126470A1 | 2018-07-12 |
Foreign References:
| CN107922929A | 2018-04-17 | |||
| CN107002051A | 2017-08-01 | |||
| CN108018270A | 2018-05-11 | |||
| US20070048748A1 | 2007-03-01 |
Other References:
HOSHINO, H. ET AL.: "DNA Polymerase Variants with High Processivity and Accuracy for Encoding and Decoding Locked Nucleic Acid Sequences", J. AM. CHEM. SOC., vol. 142, 11 December 2020 (2020-12-11), XP093002921, DOI: 10.1021/jacs.0c10902
LIU CHAO, COZENS CHRISTOPHER, JAZIRI FATEN, ROZENSKI JEF, MARÉCHAL AMANDINE, DUMBRE SHRINIVAS, PEZO VALÉRIE, MARLIÈRE PHILIPPE, PI: "Phosphonomethyl Oligonucleotides as Backbone-Modified Artificial Genetic Polymers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 140, no. 21, 30 May 2018 (2018-05-30), pages 6690 - 6699, XP093007481, ISSN: 0002-7863, DOI: 10.1021/jacs.8b03447
LIU CHAO, COZENS CHRISTOPHER, JAZIRI FATEN, ROZENSKI JEF, MARÉCHAL AMANDINE, DUMBRE SHRINIVAS, PEZO VALÉRIE, MARLIÈRE PHILIPPE, PI: "Phosphonomethyl Oligonucleotides as Backbone-Modified Artificial Genetic Polymers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 140, no. 21, 30 May 2018 (2018-05-30), pages 6690 - 6699, XP093007481, ISSN: 0002-7863, DOI: 10.1021/jacs.8b03447
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
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