Title:
TRANSAMINASE MUTANT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/123589
Kind Code:
A1
Abstract:
Disclosed are a transaminase mutant and the use thereof, wherein the transaminase mutant has a sequence formed after an amino acid mutation occurs in the sequence shown in SEQ ID NO: 1, wherein the site at which the amino acid mutation occurs includes a V242W site. On the basis of transaminase shown in SEQ ID NO: 1, the transaminase mutant undergoes mutation by means of a site-directed mutation method, thereby changing the amino acid sequence of transaminase and achieving changes in protein structure and function, and then the transaminase with the above-mentioned mutation site is obtained by means of a directed screening method. The obtained transaminase has relatively high catalytic activity, specific selectivity, wide substrate spectra and broad industrial prospects.
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Inventors:
HONG HAO (US)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
LI RUI (CN)
YANG YIMING (CN)
ZHANG YANQING (CN)
JAMES GAGE (US)
ZHANG NA (CN)
JIAO XUECHENG (CN)
LI RUI (CN)
YANG YIMING (CN)
ZHANG YANQING (CN)
Application Number:
PCT/CN2022/073561
Publication Date:
July 06, 2023
Filing Date:
January 24, 2022
Export Citation:
Assignee:
ASYMCHEM LAB TIANJIN CO LTD (CN)
International Classes:
C12N9/10; C12N1/19; C12N1/21; C12N15/54; C12P13/00; C12P17/00; C12P17/04
Domestic Patent References:
| WO2006063336A2 | 2006-06-15 | |||
| WO2020237552A1 | 2020-12-03 |
Foreign References:
| CN110205310A | 2019-09-06 | |||
| CN112888780A | 2021-06-01 | |||
| CN111549011A | 2020-08-18 | |||
| CN110592042A | 2019-12-20 | |||
| CN110628742A | 2019-12-31 |
Other References:
MARTIN, A. R. ET AL.: "Improved activity and thermostability of (S)-aminotransferase by error-prone polymerase chain reaction for the production of a chiral amine", BIOCHEMICAL ENGINEERING JOURNAL, vol. 37, 31 December 2007 (2007-12-31), pages 246 - 255, XP002473281, DOI: 10.1016/j.bej.2007.05.001
SVEDENDAHL, M. ET AL.: "Reversed Enantiopreference of an ω-Transaminase by a Single-Point Mutation", CHEMCATCHEM, vol. 2, 22 July 2010 (2010-07-22), pages 976 - 980, XP002730784, DOI: 10.1002/cctc.201000107
GAO, XINXING ET AL.: "Advances in Molecular Modification of ω-transaminase", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 7, 25 July 2018 (2018-07-25), pages 1057 - 1068, XP055762590, DOI: 10.13345/j.cjb.170455
JIA, HONGHUA ET AL.: "Progress in ω-transaminase in Chiral Amine Synthesis", MODERN CHEMICAL INDUSTRY, vol. 32, no. 3, 31 March 2012 (2012-03-31), pages 16 - 22, XP008183346
SVEDENDAHL, M. ET AL.: "Reversed Enantiopreference of an ω-Transaminase by a Single-Point Mutation", CHEMCATCHEM, vol. 2, 22 July 2010 (2010-07-22), pages 976 - 980, XP002730784, DOI: 10.1002/cctc.201000107
GAO, XINXING ET AL.: "Advances in Molecular Modification of ω-transaminase", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 34, no. 7, 25 July 2018 (2018-07-25), pages 1057 - 1068, XP055762590, DOI: 10.13345/j.cjb.170455
JIA, HONGHUA ET AL.: "Progress in ω-transaminase in Chiral Amine Synthesis", MODERN CHEMICAL INDUSTRY, vol. 32, no. 3, 31 March 2012 (2012-03-31), pages 16 - 22, XP008183346
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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