Title:
TRANSFORMED CELL PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/244895
Kind Code:
A1
Abstract:
Provided is a transformed cell production method comprising an introduction step of bringing an introduction liquid containing a molecule of interest into contact with a target cell to introduce the molecule of interest into the target cell. In the introduction step, after the molecule of interest is introduced into the target cell by endocytosis and a trait is conferred, deleted or maintained by the introduced molecule of interest, the foreign molecule of interest (e.g. vector) does not remain in the established cell.
Inventors:
JINNO MASAFUMI (JP)
KATO HIDEMASA (JP)
TOKUZAWA YOSHIMI (JP)
ONISHI AKIHITO (JP)
MYOJO JUNKO (JP)
SATOH SUSUMU (JP)
KATO HIDEMASA (JP)
TOKUZAWA YOSHIMI (JP)
ONISHI AKIHITO (JP)
MYOJO JUNKO (JP)
SATOH SUSUMU (JP)
Application Number:
PCT/JP2019/024134
Publication Date:
December 26, 2019
Filing Date:
June 18, 2019
Export Citation:
Assignee:
PEARL KOGYO CO LTD (JP)
YS CORP (JP)
UNIV NAT CORP EHIME UNIV (JP)
YS CORP (JP)
UNIV NAT CORP EHIME UNIV (JP)
International Classes:
C12N5/10; C12N15/87
Domestic Patent References:
| WO2002064767A1 | 2002-08-22 | |||
| WO2014208425A1 | 2014-12-31 | |||
| WO2017123789A1 | 2017-07-20 | |||
| WO2002064767A1 | 2002-08-22 |
Foreign References:
| JP2010220517A | 2010-10-07 | |||
| JP2017189158A | 2017-10-19 | |||
| JP2013255475A | 2013-12-26 | |||
| JP2013255474A | 2013-12-26 | |||
| JP2013255475A | 2013-12-26 | |||
| JP2010220517A | 2010-10-07 | |||
| JP2013255474A | 2013-12-26 |
Other References:
KANEKO, TOSHIRO; SHOTA, SASAKI; HIDEAKI, KONISHI; HIROYASU, ISHIDA; MAKOTO, KANZAKI: "29p-B7-4 Generation of hydroxyl radical controlled plasma and its application to gene transfection", 60TH JSAP SPRING MEETING; JAPAN SOCIETY OF APPLIED PHYSICS, KANAGAWA (JAPAN), 27-30 MARCH 2013, vol. 60, 27 March 2013 (2013-03-27), pages 08-128, XP009525335
SATO, SUSUMU: "30p-L-5: Practical Application of plasma technology in Molecular Biology - Research and Development of New molecular Delivery System", 56TH CONFERENCE OF THE JAPAN SOCIETY OF APPLIED PHYSICS AND THE RELATED SOCIETIES; 56 (TSUKUBA) : 2009.03.30-04.01, 30 March 2009 (2009-03-30), pages 25, XP009525334
YU ET AL., SCIENCE, 2009, pages 324
UEHARA ET AL., ANN CLIN TRANSL NEUROL., 2014, pages 361 - 369
See also references of EP 3808839A4
SATO, SUSUMU: "30p-L-5: Practical Application of plasma technology in Molecular Biology - Research and Development of New molecular Delivery System", 56TH CONFERENCE OF THE JAPAN SOCIETY OF APPLIED PHYSICS AND THE RELATED SOCIETIES; 56 (TSUKUBA) : 2009.03.30-04.01, 30 March 2009 (2009-03-30), pages 25, XP009525334
YU ET AL., SCIENCE, 2009, pages 324
UEHARA ET AL., ANN CLIN TRANSL NEUROL., 2014, pages 361 - 369
See also references of EP 3808839A4
Attorney, Agent or Firm:
FUKAMI PATENT OFFICE, P.C. (JP)
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