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Title:
TRANSPOSON MEDIATED MULTIPLEX SEQUENCING
Document Type and Number:
WIPO Patent Application WO2002004674
Kind Code:
A3
Abstract:
The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

Inventors:
AUGUST PAUL R (US)
KEAGLE PAMELA J (US)
LONG HENRY (US)
WIENCIS ANNA (US)
CALL KATHERINE (US)
DRAPER MICHAEL (US)
Application Number:
PCT/US2001/021269
Publication Date:
April 17, 2003
Filing Date:
July 05, 2001
Export Citation:
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Assignee:
AVENTIS PHARMA INC (US)
AUGUST PAUL R (US)
KEAGLE PAMELA J (US)
LONG HENRY (US)
WIENCIS ANNA (US)
CALL KATHERINE (US)
DRAPER MICHAEL (US)
International Classes:
C12N15/09; C12N15/10; C12Q1/68; C12Q1/6806; C12Q1/6869; G01N33/50; C12Q; (IPC1-7): C12Q1/68; C12N15/10
Domestic Patent References:
WO1998037205A11998-08-27
WO1997046714A11997-12-11
WO2000026243A22000-05-11
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