Title:
TWO-DIMENSIONAL CELL ARRAY DEVICE AND APPARATUS FOR GENE QUANTIFICATION AND SEQUENCE ANALYSIS
Document Type and Number:
WIPO Patent Application WO/2014/141386
Kind Code:
A1
Abstract:
To express a number of genes in a number of cells, it was necessary to separate the cells, extract the genes therefrom, then amplify nucleic acids, and analyze the expression of the genes by sequence analysis. However, separation of cells seriously injures the cells and an expensive system is required therefor.
According to the present invention, the expression of genes in individual cells is highly accurately analyzed by: arranging a pair of structures including a cell-trapping section and a nucleic acid-trapping section in a vertical direction to extract individual genes in cells; synthesizing cDNAs in the nucleic acid-trapping section; amplifying nucleic acids; and then analyzing the sequences thereof with a next generation sequencer.
Inventors:
SHIRAI MASATAKA (JP)
KAMBARA HIDEKI (JP)
TANIGUCHI KIYOMI (JP)
TANABE MAIKO (JP)
KAMBARA HIDEKI (JP)
TANIGUCHI KIYOMI (JP)
TANABE MAIKO (JP)
Application Number:
PCT/JP2013/056818
Publication Date:
September 18, 2014
Filing Date:
March 12, 2013
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12N15/09; C12M1/00; C12Q1/68
Domestic Patent References:
| WO2011068088A1 | 2011-06-09 | |||
| WO2009016842A1 | 2009-02-05 |
Foreign References:
| JP2007089566A | 2007-04-12 | |||
| JP2008539711A | 2008-11-20 | |||
| JP2009276883A | 2009-11-26 |
Other References:
WHITE, A.K. ET AL.: "High-throughput microfluidic single- cell RT-qPCR.", PROC. NATL. ACAD. SCI. USA, vol. 108, no. 34, 23 August 2011 (2011-08-23), pages 13999 - 14004, XP055152556
YIN, H. ET AL.: "Microfluidics for single cell analysis.", CURR. OPIN. BIOTECHNOL., vol. 23, no. 1, February 2012 (2012-02-01), pages 110 - 119, XP055279650
HSIAO, A.P. ET AL.: "Microfluidic device for capture and isolation of single cells.", PROC. OF SPIE, vol. 7759, 2010, pages 77590W-1 - 77590W-9, XP055279658
LINDSTROM, S. ET AL.: "Overview of single- cell analyses: microdevices and applications.", LAB ON A CHIP, vol. 10, no. 24, 21 December 2010 (2010-12-21), pages 3363 - 3372, XP055000729
NATURE METHOD, vol. 6, no. 7, 2009, pages 503 - 506
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 34, 2011, pages 13999 - 14004
BIOMATERIALS, 2009, pages 4930 - 4938
LAB CHIP, vol. 7, 2007, pages 199 - 206
YIN, H. ET AL.: "Microfluidics for single cell analysis.", CURR. OPIN. BIOTECHNOL., vol. 23, no. 1, February 2012 (2012-02-01), pages 110 - 119, XP055279650
HSIAO, A.P. ET AL.: "Microfluidic device for capture and isolation of single cells.", PROC. OF SPIE, vol. 7759, 2010, pages 77590W-1 - 77590W-9, XP055279658
LINDSTROM, S. ET AL.: "Overview of single- cell analyses: microdevices and applications.", LAB ON A CHIP, vol. 10, no. 24, 21 December 2010 (2010-12-21), pages 3363 - 3372, XP055000729
NATURE METHOD, vol. 6, no. 7, 2009, pages 503 - 506
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 108, no. 34, 2011, pages 13999 - 14004
BIOMATERIALS, 2009, pages 4930 - 4938
LAB CHIP, vol. 7, 2007, pages 199 - 206
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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