Title:
UNNATURAL AMINO ACID, AND USE THEREOF, RECOMBINANT PROTEIN CONTAINING SAME, AND RECOMBINANT PROTEIN CONJUGATE
Document Type and Number:
WIPO Patent Application WO/2023/004686
Kind Code:
A1
Abstract:
Provided is an unnatural amino acid, which is a compound having a structure as shown in formula (I) or an enantiomer thereof. Also provided is the use of the unnatural amino acid. Further provided is a recombinant protein containing the unnatural amino acid and a protein conjugate prepared from the recombinant protein. The unnatural amino acid is easy to prepare and has a good safety; when the unnatural amino acid is inserted into a protein, the protein is not easily inactivated; the unnatural amino acid has a high binding rate to a conjugated moiety, and the stability of the obtained conjugate is higher; and the unnatural amino acid can be used in numerous fields, especially in the preparation of recombinant proteins or recombinant protein conjugates.
Inventors:
YANG JINWEI (CN)
YE CHENGHAO (CN)
XIA GANG (CN)
HUO PENGCHAO (CN)
DING WEN (CN)
CHEN LONGFEI (CN)
JIAO LIN (CN)
HUANG HAO (CN)
HENG XIN (CN)
GONG LIYING (CN)
ZHU JINGJING (CN)
YING YUEBIN (CN)
LIANG XUEJUN (CN)
YE CHENGHAO (CN)
XIA GANG (CN)
HUO PENGCHAO (CN)
DING WEN (CN)
CHEN LONGFEI (CN)
JIAO LIN (CN)
HUANG HAO (CN)
HENG XIN (CN)
GONG LIYING (CN)
ZHU JINGJING (CN)
YING YUEBIN (CN)
LIANG XUEJUN (CN)
Application Number:
PCT/CN2021/109276
Publication Date:
February 02, 2023
Filing Date:
July 29, 2021
Export Citation:
Assignee:
NOVOCODEX BIOPHARMACEUTICALS CO LTD (CN)
International Classes:
C07C271/22; A61K38/27; A61K47/54; A61K47/60; A61P5/06; C07C235/20; C07C235/78; C07C237/12; C07K14/61
Foreign References:
| CN102264697A | 2011-11-30 | |||
| CN102838671A | 2012-12-26 | |||
| CN101090980A | 2007-12-19 | |||
| CN101341118A | 2009-01-07 | |||
| CN112279906A | 2021-01-29 | |||
| CN106146663A | 2016-11-23 | |||
| CN104812734A | 2015-07-29 | |||
| US20090011459A1 | 2009-01-08 | |||
| CN113260374A | 2021-08-13 | |||
| CN102838671B | 2014-06-11 | |||
| CN106146663A | 2016-11-23 | |||
| CN103153927B | 2017-02-15 | |||
| CN202011264982A | 2020-11-12 | |||
| CN112279906A | 2021-01-29 |
Other References:
DATABASE REGISTRY ANONYMOUS : " L-Lysine, N6-[3-(acetyloxy)benzoyl]- (CA INDEX NAME) ", XP093028481, retrieved from STN
DATABASE REGISTRY ANONYMOUS : " L-Lysine, N6-[2,3-bis(acetyloxy)benzoyl]- (CA INDEX NAME) ", XP093028485, retrieved from STN
DATABASE REGISTRY ANONYMOUS : "L-Lysine, N6-[4-(acetylamino)benzoyl]- (CA INDEX NAME) ", XP093028487, retrieved from STN
DATABASE REGISTRY ANONYMOUS : " L-Lysine, N6-(4-acetylbenzoyl)- (CA INDEX NAME)", XP093028490, retrieved from STN
SHOZEN, N. ET AL.: "Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 19, 25 July 2009 (2009-07-25), pages 4909 - 4911, XP026458522
J. AM. CHEM. SOC., vol. 131, 2009, pages 8720
LIHUA SONG ET AL., JOURNAL OF BIOLOGY, vol. 16, no. 1, 1999, pages 6 - 8
DATABASE REGISTRY ANONYMOUS : " L-Lysine, N6-[2,3-bis(acetyloxy)benzoyl]- (CA INDEX NAME) ", XP093028485, retrieved from STN
DATABASE REGISTRY ANONYMOUS : "L-Lysine, N6-[4-(acetylamino)benzoyl]- (CA INDEX NAME) ", XP093028487, retrieved from STN
DATABASE REGISTRY ANONYMOUS : " L-Lysine, N6-(4-acetylbenzoyl)- (CA INDEX NAME)", XP093028490, retrieved from STN
SHOZEN, N. ET AL.: "Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 19, 25 July 2009 (2009-07-25), pages 4909 - 4911, XP026458522
J. AM. CHEM. SOC., vol. 131, 2009, pages 8720
LIHUA SONG ET AL., JOURNAL OF BIOLOGY, vol. 16, no. 1, 1999, pages 6 - 8
Attorney, Agent or Firm:
SINO-CREATIVITY INTELLECTUAL PROPERTY LAW FIRM (CN)
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