Title:
USE OF POX4 PROMOTER TO INCREASE GENE EXPRESSION IN CANDIDA TROPICALIS
Document Type and Number:
WIPO Patent Application WO2004013336
Kind Code:
A3
Abstract:
The present invention provides methods of converting or increasing conversion of a fatty acid to its corresponding dicarboxylic acid. The methods comprise isolating a promoter from a yeast gene which gene is induced when the yeast is grown on a fatty acid or alkane substrate, and operably linking the promoter to a gene involved in dicarboxylic acid production to form an expression vector. Yeast cells are subsequently transformed with such an expression vector and cultured in a media containing an organic substrate biooxidizable to a mono- or polycarboxylic acid, and resultant yeast cells convert or increase conversion of fatty acids to their corresponding dicarboxylic acids. Examples of promoters that may be used in the methods of the present invention include those from C. tropicalis catalase, citrate synthase, 3-ketoacyl-CoA thiolase A, citrate synthase, O-acetylhornserine sulphydrylase, protease, carnitine O-acetyltransferase, hydratase-dehydrogenase, and epimerase genes. A preferred promoter for use in a subject expression vector is the POX4 gene promoter. Examples of genes involved in dicarboxylic acid production include members of an omega-hydroxylase complex such as e.g., CYP, NCP, or CYTb5 genes. Host cells comprising such expression vectors are also provided. Preferred host cells are include Yarrowia, Candida, Bebaromyces, Saccharomyces, Schizosaccharomyces, and Pichia.
Inventors:
ZHANG YEYAN
WILSON RON C
CRAFT DAVID L
EIRICH DUDLEY L
FRAYER ROBERT W
WILSON RON C
CRAFT DAVID L
EIRICH DUDLEY L
FRAYER ROBERT W
Application Number:
PCT/US2003/024262
Publication Date:
September 30, 2004
Filing Date:
August 04, 2003
Export Citation:
Assignee:
COGNIS CORP (US)
International Classes:
C12N15/81; C12P7/44; (IPC1-7): C12P7/44; C12N15/81
Foreign References:
| US6331420B1 | 2001-12-18 | |||
| US6503734B1 | 2003-01-07 |
Other References:
OKAZAKI K. ET AL: "Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence", PNAS, vol. 83, no. 5, March 1986 (1986-03-01), pages 1232 - 1236, XP002979085
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