Title:
Variant galactose oxidase,nucleic acid encoding same,and methods of using same
Document Type and Number:
WIPO Patent Application WO2001062938
Kind Code:
A3
Abstract:
The present invention provides mutant genes encoding variant galactose oxidase enzymes, termed vGOs; constructs and recombinant host cells incorporating the genes; the vGO polypeptides encoded by the genes; antibodies to the polypeptides; and methods of making and using all of the foregoing.
Inventors:
MAFFIA ANTHONY M III (US)
DELAGRAVE SIMON (US)
MURPHY DENNIS J (US)
RITTENHOUSE PRUSS JENNIFER (US)
BYLINA EDWARD (US)
COLEMAN WILLIAM J (US)
DELAGRAVE SIMON (US)
MURPHY DENNIS J (US)
RITTENHOUSE PRUSS JENNIFER (US)
BYLINA EDWARD (US)
COLEMAN WILLIAM J (US)
Application Number:
PCT/US2001/005732
Publication Date:
April 25, 2002
Filing Date:
February 21, 2001
Export Citation:
Assignee:
HERCULES INC (US)
International Classes:
C12N9/04; C12N15/53; D21H17/32; (IPC1-7): C12N15/53; C12N9/04; C12N15/63; C12N5/10; C07K16/40; D21H17/32
Foreign References:
| US5502091A | 1996-03-26 |
Other References:
ANDREW J BARON ET AL: "Structure and mechanism of Galactose oxidase", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 269, no. 40, 7 October 1994 (1994-10-07), pages 25095 - 25105, XP002139974, ISSN: 0021-9258
MICHAEL J MCPHERSON ET AL: "Galactose Oxidase of Dactylium dendroides", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 267, no. 12, Q01745, 25 April 1992 (1992-04-25), pages 8146 - 8152, XP002139972, ISSN: 0021-9258, Retrieved from the Internet
DELAGRAVE AND D C YOUVAN S: "Searching sequence space to engineer proteins: Exponential ensemble mutagenesis", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 11, no. 11, December 1993 (1993-12-01), pages 1548 - 1552, XP002162579, ISSN: 1087-0156
STEMMER W P C: "RAPID EVOLUTION OF A PROTEIN IN VITRO BY DNA SHUFFLING", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 370, 4 August 1994 (1994-08-04), pages 389 - 391, XP002912216, ISSN: 0028-0836
MICHAEL J MCPHERSON ET AL: "Galactose Oxidase of Dactylium dendroides", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 267, no. 12, Q01745, 25 April 1992 (1992-04-25), pages 8146 - 8152, XP002139972, ISSN: 0021-9258, Retrieved from the Internet
DELAGRAVE AND D C YOUVAN S: "Searching sequence space to engineer proteins: Exponential ensemble mutagenesis", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 11, no. 11, December 1993 (1993-12-01), pages 1548 - 1552, XP002162579, ISSN: 1087-0156
STEMMER W P C: "RAPID EVOLUTION OF A PROTEIN IN VITRO BY DNA SHUFFLING", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 370, 4 August 1994 (1994-08-04), pages 389 - 391, XP002912216, ISSN: 0028-0836
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