VIRUS DETECTION METHOD USING WELL ARRAY, WELL ARRAY, AND DETECTION DEVICE

WIPO Patent Application WO/2022/202217

A1

[Problem] To detect a virus quickly and with a high degree of sensitivity. [Solution] A virus detection method for using a well array, in which a plurality of wells are formed such that adjacent wells are defined by lateral walls that rise on a substrate, to detect a virus in a test liquid on the basis of detected light emission from the wells, wherein the well array has a detection region where a well formation surface area, which is the substrate surface area of the region where the wells are formed, is at least 5 mm2, the overall well volume obtained by adding the volumes of the individual wells is at least 17 nL, the depth of the wells is 3.5-40 μm, the opening surface areas of the wells are 18-1,700 μm 2, the volumes of the wells are 74-6,000 fL, and the lateral wall thicknesses between adjacent wells are 0.5-15 μm, and the light emission from the wells is detected using a detection unit having an observation field of view fixed at a size including the detection region.

FUJIMAKI MAKOTO (JP)
ASHIBA HIROKI (JP)

PCT/JP2022/009368

September 29, 2022

March 04, 2022

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AIST (JP)

G01N21/64; C12M1/34; C12Q1/04; C12Q1/70; G01N21/03; G01N21/78; G01N33/569; G01N37/00

WO2018181488A1	2018-10-04
WO2016006208A1	2016-01-14
WO2016006208A1	2016-01-14
WO2012121310A1	2012-09-13

JP5551798B2	2014-07-16
JP2018091737A	2018-06-14
JP3886161B2	2007-02-28
JP5551798B2	2014-07-16
JP5363663B2	2013-12-11
JP2018038384A	2018-03-15
JP2010112777A	2010-05-20

MINAGAWA YOSHIHIRO, UENO HIROSHI, TABATA KAZUHITO V., NOJI HIROYUKI: "Mobile imaging platform for digital influenza virus counting", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, UK, vol. 19, no. 16, 6 August 2019 (2019-08-06), UK , pages 2678 - 2687, XP055969681, ISSN: 1473-0197, DOI: 10.1039/C9LC00370C
LEIRS KAREN, TEWARI KUMAR PHALGUNI, DECROP DEBORAH, PÉREZ-RUIZ ELENA, LEBLEBICI PELIN, VAN KELST BRAM, COMPERNOLLE GRIET, MEEUWS H: "Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 88, no. 17, 6 September 2016 (2016-09-06), US , pages 8450 - 8458, XP055969682, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.6b00502
KAZUHITO V. TABATA, YOSHIHIRO MINAGAWA, YUKO KAWAGUCHI, MANA ONO, YOSHIKI MORIIZUMI, SEIYA YAMAYOSHI, YOICHIRO FUJIOKA, YUSUKE OHB: "Antibody-free digital influenza virus counting based on neuraminidase activity", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 December 2019 (2019-12-01), XP055676628, DOI: 10.1038/s41598-018-37994-6
ENZYME-LINKED IMMUNO SORBENT ASSAY
DAVID M RISSIN ET AL., NATURE BIOTECHNOLOGY, vol. 28, no. 6, 2010, pages 595
ELENA PEREZ-RUIZ ET AL., ANALYTICA CHIMICA ACTA, vol. 1015, 2018, pages 74
SOO HYEON KIM ET AL., LAB ON A CHIP, vol. 12, 2012, pages 4986
DAVID M RISSIN ET AL., ANALYTICAL CHEMISTRY, vol. 83, 2011, pages 2279
STEPHANIE M. SCHUBERT ET AL., ANALYTICAL CHEMISTRY, vol. 88, 2016, pages 8450
YOSHIHIRO MINAGAWA ET AL., LAB ON A CHIP, vol. 19, 2019, pages 2678
KAZUHITO V. TABATA ET AL., SCIENTIFIC REPORTS, vol. 9, 2019, pages 1067
H SHI ET AL., JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 134, 2012, pages 9569

SHIODA Shin (JP)

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