Title:
ZEBRAFISH MODEL OF HUMAN ACUTE MYELOID LEUKEMIA AND METHOD OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/236903
Kind Code:
A1
Abstract:
Genetically modified zebrafish, in which mutation combinations frequently identified in human AML are stably expressed in the stem cell population of the fish, are provided. The combination of mutations result in morphologic, cytochemical and molecular changes of its blood cells that are remarkably similar to those in human AML. The zebrafish model provides a foundation for the study of AML initiation and progression and a high throughput in vivo drug screening platform to identify personalized therapies for AML based on specific mutation combinations. The method of drug screening includes contacting embryos or adult fish containing mutations as disclosed herein, with a test agent, at test concentrations and test intervals to determine the therapeutic effect if any, of the test agent.
Inventors:
LEUNG ANSKAR YU HUNG (CN)
WANG DANDAN (CN)
SUN XUAN (CN)
MA CHUN-HANG ALVIN (CN)
CHENG YIK LING BOWIE (CN)
HE BAILIANG (CN)
WANG DANDAN (CN)
SUN XUAN (CN)
MA CHUN-HANG ALVIN (CN)
CHENG YIK LING BOWIE (CN)
HE BAILIANG (CN)
Application Number:
PCT/CN2023/098302
Publication Date:
December 14, 2023
Filing Date:
June 05, 2023
Export Citation:
Assignee:
VERSITECH LTD (CN)
International Classes:
C12N15/85; A01K67/027; A61K49/00
Foreign References:
| CN103977424A | 2014-08-13 | |||
| CN108531510A | 2018-09-14 | |||
| US20070186288A1 | 2007-08-09 | |||
| US20040117867A1 | 2004-06-17 |
Other References:
SKAYNEH, JISHI, HLEIHEL, HAMIEH, DARWICHE, BAZARBACHI, EL SABBAN, EL HAJJ: "A Critical Review of Animal Models Used in Acute Myeloid Leukemia Pathophysiology", GENES, MDPI AG, US, vol. 10, no. 8, US , pages 614, XP093115067, ISSN: 2073-4425, DOI: 10.3390/genes10080614
XIANG WENBI; HE ZHIXU; SHU LIPING: "Research Progress of Zebrafish Leukemia Model", MEDICAL RECAPITULATE, YIXUE ZONGSHU, CN, vol. 26, no. 2, 31 January 2020 (2020-01-31), CN , pages 263 - 268, XP009550979, ISSN: 1006-2084
XIANG WENBI; HE ZHIXU; SHU LIPING: "Research Progress of Zebrafish Leukemia Model", MEDICAL RECAPITULATE, YIXUE ZONGSHU, CN, vol. 26, no. 2, 31 January 2020 (2020-01-31), CN , pages 263 - 268, XP009550979, ISSN: 1006-2084
Attorney, Agent or Firm:
LIU , SHEN & ASSOCIATES (CN)
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