Title:
iPS PRECURSOR CELL AND iPS CELL
Document Type and Number:
WIPO Patent Application WO/2014/167866
Kind Code:
A1
Abstract:
Provided is an iPS precursor cell capable of highly efficient transgenesis as a result of stable maintenance cultivation. The precursor cell is prepared by introducing either OCT4, SOX2, or KLF4 to a human somatic cell, and culturing same in a medium including an MEK inhibitor, a GSK3β inhibitor, and a leukemia inhibitor factor (LIF). The MEK inhibitor is, for example, PD0325901. The GSK3β inhibitor is, for example, CHIR99021. The iPS precursor cell is OCT4 negative, NANOG negative, and NESTIN positive.
Inventors:
AKUTSU HIDENORI (JP)
UMEZAWA AKIHIRO (JP)
MIURA TAKUMI (JP)
UMEZAWA AKIHIRO (JP)
MIURA TAKUMI (JP)
Application Number:
PCT/JP2014/002089
Publication Date:
October 16, 2014
Filing Date:
April 11, 2014
Export Citation:
Assignee:
JAPAN HEALTH SCIENCE FOUND (JP)
International Classes:
C12N5/10; C12N5/071
Other References:
HIRANO K. ET AL.: "Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitors", STEM CELLS AND DEVELOPMENT, vol. 21, 2012, pages 1287 - 1298
HANNA J. ET AL.: "Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs", PNAS, vol. 107, 2010, pages 9222 - 9227
SILVA J. ET AL.: "Nanog Is the Gateway to the Pluripotent Ground State", CELL, vol. 138, 2009, pages 722 - 737
THEUNISSEN T.W. ET AL.: "Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions", CURRENT BIOLOGY, vol. 21, 2011, pages 65 - 71
GORDEEVA O.F. ET AL.: "Expression patterns of germ line specific genes in mouse and human pluripotent stem cells are associated with regulation of ground and primed state of pluripotency", RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 42, 2011, pages 355 - 375
NICHOLS J. ET AL.: "Naive and Primed Pluripotent States", CELL STEM CELL, vol. 4, 2009, pages 487 - 492
GAFNI O. ET AL.: "Derivation of novel human ground state naive pluripotent stem cells", NATURE, vol. 504, pages 282 - 286
HANNA J. ET AL.: "Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs", PNAS, vol. 107, 2010, pages 9222 - 9227
SILVA J. ET AL.: "Nanog Is the Gateway to the Pluripotent Ground State", CELL, vol. 138, 2009, pages 722 - 737
THEUNISSEN T.W. ET AL.: "Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions", CURRENT BIOLOGY, vol. 21, 2011, pages 65 - 71
GORDEEVA O.F. ET AL.: "Expression patterns of germ line specific genes in mouse and human pluripotent stem cells are associated with regulation of ground and primed state of pluripotency", RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 42, 2011, pages 355 - 375
NICHOLS J. ET AL.: "Naive and Primed Pluripotent States", CELL STEM CELL, vol. 4, 2009, pages 487 - 492
GAFNI O. ET AL.: "Derivation of novel human ground state naive pluripotent stem cells", NATURE, vol. 504, pages 282 - 286
Attorney, Agent or Firm:
MAEDA & PARTNERS (JP)
Patent business corporation MAEDA PATENT OFFICE (JP)
Patent business corporation MAEDA PATENT OFFICE (JP)
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