Title:
【発明の名称】ヒトのシトクロムP450 3A4に対する抗ペプチド抗体
Document Type and Number:
Japanese Patent JP2000513742
Kind Code:
A
Abstract:
Anti-peptide antibody raised against a portion of human cytochrome P450 3A4 is disclosed. A particular anti-peptide antibody was raised against a 21-amino acid peptide corresponding to residues 253-273 of human cytochrome P450 3A4. High titer antibodies were produced by rabbits immunized with this peptide as judged by ELISA. This anti-peptide antibody is specific for human CYP3A4 and exhibited greater than 90-95% inhibition of testosterone 6beta-hydroxylation, while other cytochrome P450-mediated reactions in human liver microsomes were not inhibited. An inhibitory epitope has been mapped within amino acids 261-267 of human CYP3A4.
Inventors:
Lou, Anthony YH
Wang, Regina Davryu
Wang, Regina Davryu
Application Number:
JP54313898A
Publication Date:
October 17, 2000
Filing Date:
April 09, 1998
Export Citation:
Assignee:
Merck End Company, Inc.
International Classes:
C12N9/99; A61K39/00; A61K39/385; A61K39/395; A61P43/00; C07K14/47; C07K16/40; C12N9/02; C12P21/08; (IPC1-7): C07K14/47; C07K16/40; C12N9/99
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)