Title:
【発明の名称】固相合成のための酵素切断可能なリンカー
Document Type and Number:
Japanese Patent JP2000514432
Kind Code:
A
Abstract:
An enzyme cleavable linker is prepared on which organic compounds are synthesized when the linker is bound to a solid phase. The linker contains a functional group on which a synthesized organic compound is bound when synthesis takes place, and a recognition site for a hydrolytic enzyme. Reacting the linker with the enzyme causes the linker to fragment at a site different from the recognition site to liberate the synthesized organic compound. The solid phase may be a crosslinked polyacrylamide containing an amino group for attaching the linker, and the linker is bound to the solid phase via a spacer. The spacer is attached to the solid phase by an ester, ether, amide or amine linkage, or a sulfide or phosphate linkage. In a specific reaction of forming a solid phase containing the linker, 2-acetoxy-5-hydroxymethylbenzoic acid is attached to an amino group-containing polymer via a spacer followed by conversion to a chloroformic ester.
Inventors:
Herbert Waldmann
Bernd Sauerbrih
Uve Greater
Bernd Sauerbrih
Uve Greater
Application Number:
JP50471698A
Publication Date:
October 31, 2000
Filing Date:
June 27, 1997
Export Citation:
Assignee:
BASF Akchen Gezel Shaft
International Classes:
C07B61/00; C07C235/12; C07C235/66; C07K1/04; C12P1/00; C12P13/04; (IPC1-7): C07B61/00
Attorney, Agent or Firm:
Toshio Yano (3 outside)