Title:
【発明の名称】迅速な微生物プロテアーゼ検定法
Document Type and Number:
Japanese Patent JP2001502162
Kind Code:
A
Abstract:
An assay for detecting microbial protease activity in clinical and laboratory samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired protease activity; immobilizing the microorganisms in the sample on a solid phase substrate; contacting the immobilized microorganisms with an enzymatic substrate producing an enzymatic substrate end-product; contacting the enzymatic substrate end-product with a chemical enhancing reagent producing a detectable chromogenic reaction which varies in intensity with the level of protease activity in the sample; and detecting the chromogenic reaction whereby the semi-quantitative presence of the protease activity in the sample is determined. The device for conducting these assays is a frame or support which holds a solid phase substrate capable of binding the sought microorganisms of interest while permitting drainage of other materials or fluids, which may contain host proteases, away from the immobilized microorganisms.
Inventors:
Stephen Alden Rals
Lloyd Grant Simonson
Sylvia Zotzo Shade
Lloyd Grant Simonson
Sylvia Zotzo Shade
Application Number:
JP52521997A
Publication Date:
February 20, 2001
Filing Date:
December 23, 1996
Export Citation:
Assignee:
THE UNITED STATES OF AMERICA
International Classes:
C12Q1/37; (IPC1-7): C12Q1/37
Attorney, Agent or Firm:
Kenji Doi (1 person outside)