Title:
【発明の名称】BMP―4プロモーターならびに骨粗鬆症の予防および/または治療用の治療剤のスクリーニングにおけるその用途
Document Type and Number:
Japanese Patent JP2001509010
Kind Code:
A
Abstract:
PCT No. PCT/EP97/06668 Sec. 371 Date Jun. 21, 1999 Sec. 102(e) Date Jun. 21, 1999 PCT Filed Nov. 20, 1997 PCT Pub. No. WO98/23740 PCT Pub. Date Jun. 4, 1998The isolated human bone morphogenic protein-4 (BMP-4) promoter region, a vector comprising the isolated promoter region, and host cells comprising the isolated promoter region is described. A method for the identification of potential therapeutic agents for use in the prevention and/or treatment of osteoporosis, comprising the steps of: a) introducing into a cell a first expression vector comprising an isolated BMP-4 promoter region operably linked to a reporter gene, and a second expression vector comprising DNA encoding an estrogen receptor; b) contacting the cell with a candidate agent; and c) monitoring the expression of the protein encoded by the reporter gene, wherein induced expression of the protein indicates that the candidate compound is a potential therapeutic agent, is also described.
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Inventors:
Deikema, Rain
Juan Den Waynegart, Artoul
Juan Den Waynegart, Artoul
Application Number:
JP52429798A
Publication Date:
July 10, 2001
Filing Date:
November 20, 1997
Export Citation:
Assignee:
Akzo Novel N B
International Classes:
A61K38/00; A61K48/00; A61P3/14; C07K14/51; C07K14/72; C12N1/15; C12N1/19; G01N33/50; C12N1/21; C12N5/10; C12N15/09; C12N15/12; C12Q1/68; G01N33/15; (IPC1-7): C12N15/09; A61K38/00; A61P3/14; C12N1/15; C12N5/10; C12Q1/68; G01N33/15
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)