Title:
【発明の名称】発現の向上したベクター並びにその製造および使用方法
Document Type and Number:
Japanese Patent JP2001514518
Kind Code:
A
Abstract:
Disclosed and claimed are vectors having enhanced expression and methods for making and using them. Enhancement of expression is from substantially cotemporal expression of at least one first nucleic acid molecule and at least one second nucleic acid molecule. The second nucleic acid molecule encodes a transcription factor or a translation factor or a transcription factor and a translation factor. The contemporaneous expression can be from operably linking the first and second nucleic molecules to a single promoter, or from operably linking the first nucleic acid molecule to a first promoter and the second nucleic molecule to a second promoter wherein the first and second promoters function substantially contemporaneously. Thus, the first and second nucleic molecules can be at the same locus in the vector, or at different loci. The second nucleic acid can encode: one transcription factor or more than one transcription factor; or one translation factor or more than one translation factor; or at least one transcription factor and at least one translation factor. The transcription factor can be from vaccinia H4L, D6, A7, G8R, A1L, A2L, H5R, or combinations thereof. The translation factor can be from a K3L open reading frame, an E3L open reading frame, a VAI RNA, an EBER RNA, a sigma 3 open reading frame, a TRBP open reading frame, or combinations thereof. The vector can be a poxvirus such as an attenuated poxvirus, e.g., NYVAC, or ALVAC.
Inventors:
Tartagria, james
Cox, william eye
Getig, Russell Earl
Martinez, Hector
Paoletti, Enzo
Stephen Yee, Pincus
Cox, william eye
Getig, Russell Earl
Martinez, Hector
Paoletti, Enzo
Stephen Yee, Pincus
Application Number:
JP53956498A
Publication Date:
September 11, 2001
Filing Date:
February 13, 1998
Export Citation:
Assignee:
Virogenetics Corporation
International Classes:
C12N15/09; A61K35/76; A61K48/00; A61P37/04; C12N15/67; C12N15/85; C12N15/863; (IPC1-7): C12N15/09; A61K35/76; A61K48/00; A61P37/04
Attorney, Agent or Firm:
Seiji Yanagida (1 person outside)