Title:
【発明の名称】低減されたリボヌクレアーゼインヒビターアフィニティーを有するヒトリボヌクレアーゼA
Document Type and Number:
Japanese Patent JP2001520016
Kind Code:
A
Abstract:
Modified ribonucleases belonging to the RNase A superfamily of ribonucleases is disclosed. Each modified ribonuclease has a mutation in the loop region corresponding to amino acids 85-94 of bovine pancreatic RNase A. Each modified ribonuclease has reduced binding affinity for ribonuclease inhibitor (RI), wild-type ribonuclease activity, and exhibits enhanced cytotoxicity toward tumor cells, relative to the wild-type ribonuclease. Also disclosed is a method for obtaining a modified ribonuclease having reduced binding affinity for RI, wild-type ribonuclease activity, and enhanced cytotoxicity.
Inventors:
Rains Ronald Tee
Leland Peter A
Schultz El Wayne
Leland Peter A
Schultz El Wayne
Application Number:
JP2000516046A
Publication Date:
October 30, 2001
Filing Date:
October 13, 1998
Export Citation:
Assignee:
Wisconsin Alumni Research Foundation
International Classes:
C12N15/09; A61K38/46; A61P35/00; A61P43/00; C12N9/22; C12N15/55; A61K38/00; (IPC1-7): C12N15/09; A61K38/46; A61P35/00; A61P43/00; C12N9/22
Attorney, Agent or Firm:
Minoru Nakamura (9 outside)