Title:
【発明の名称】アデノ随伴ウイルス(AAV)で形質導入された遺伝子改変樹状細胞、該細胞の調製方法および該細胞の使用
Document Type and Number:
Japanese Patent JP2003506095
Kind Code:
A
Abstract:
The present invention is based upon the successful transduction of dendritic cells by an AAV vector containing a heterologous gene which is expressed in dendritic cells. The present invention is directed to a method for preparing genetically altered dendritic cells, wherein the method comprises: (a) obtaining dendritic cell precursors from a subject; and (b) transducing the dendritic cell precursors with at least one adeno-associated virus (AAV) vector comprising at least one heterologous gene to obtain genetically altered dendritic cells expressing said heterologous gene. After the transduction, the transduced precursors are incubated in a medium and under conditions whereby the dendritic cell precursors differentiate into dendritic cells that contain the heterologous gene. In a further embodiment, the present invention is directed to an adeno-associated virus (AAV) vector comprising at least a portion of the AAV genome and at least one heterologous gene selected from the group consisting of a gene encoding an antigen, a gene encoding a cytokine and combinations of one or more genes by genetically altered dendritic cells.
Inventors:
Harmonat, Paul El
Santain, Alesandro Day
Liu, Yong
Marne, michael
Param, Grosbet Peek
Kiriba-Internatay, Maurizio
Santain, Alesandro Day
Liu, Yong
Marne, michael
Param, Grosbet Peek
Kiriba-Internatay, Maurizio
Application Number:
JP2001515852A
Publication Date:
February 18, 2003
Filing Date:
August 07, 2000
Export Citation:
Assignee:
Board of Trustees of the University of Arkansas
International Classes:
C12N15/09; A61K35/12; A61K35/76; A61K38/00; A61K38/22; A61K39/12; A61K39/21; A61K39/29; A61K48/00; A61P1/16; A61P31/12; A61P31/18; A61P35/00; A61P37/04; C07K14/52; C12N5/0784; C12N5/10; C12N15/19; C12N15/864; A61K39/00; (IPC1-7): C12N15/09; A61K35/12; A61K35/76; A61K38/00; A61K38/22; A61K39/12; A61K39/21; A61K39/29; A61K48/00; A61P1/16; A61P31/12; A61P31/18; A61P35/00; A61P37/04; C12N5/10
Foreign References:
| WO1999038973A2 | 1999-08-05 |
Attorney, Agent or Firm:
Yoshio Kawaguchi (4 outside)