Title:
【発明の名称】パント-化合物を産生するための方法および微生物
Document Type and Number:
Japanese Patent JP2003527828
Kind Code:
A
Abstract:
The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzyme A biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate ±-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX , as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.
Inventors:
Yokum, Earl, Rogers
Patterson, Thomas, A
Hermann, Ciron
Pero, Janice, Gee
Patterson, Thomas, A
Hermann, Ciron
Pero, Janice, Gee
Application Number:
JP2001525331A
Publication Date:
September 24, 2003
Filing Date:
September 21, 2000
Export Citation:
Assignee:
BASF Akchen Gezel Shaft
International Classes:
C12N1/21; C12N9/02; C12N9/12; C12N9/88; C12N15/52; C12P13/02; C12P13/04; C12P13/06; C12P17/04; C12Q1/48; C12N15/09; C12R1/125; (IPC1-7): C12N15/09; C12N1/21; C12N9/02; C12N9/12; C12N9/88; C12P13/04; C12P13/06; C12Q1/48
Domestic Patent References:
| JPH06261772A | 1994-09-20 |
Foreign References:
| WO1998018954A1 | 1998-05-07 |
Other References:
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Attorney, Agent or Firm:
Sato Eto