Title:
【発明の名称】選択された遺伝子配列の部位特異的発現および/または発生上調節される発現を可能にする、より大きいヌクレオチド配列から閉環状で放出することができる構築物
Document Type and Number:
Japanese Patent JP2003528606
Kind Code:
A
Abstract:
The present invention relates generally to constructs and in particular genetic constructs comprising polynucleotide sequences capable of release in covalently closed, circular form from a larger nucleotide sequence such as, but not limited to, a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted in a form which permits expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of an extraneous nucleotide such as, but not limited to, an intronic sequence or other splice signal inserted therein. Expression and in particular transcription of the coding sequence involves splicing out the extraneous sequence. The release and circularization is generally in response to a stimulus such as a protein-mediated stimulus. More particularly, the protein is a viral or prokaryotic or eukaryotic derived protein or developmentally and/or tissue specific regulated protein.
Inventors:
Dale James Langham
Dougdale Benjamin
Hafner Greg John
Herman Scott Richard
Becker douglas kenneth
Dougdale Benjamin
Hafner Greg John
Herman Scott Richard
Becker douglas kenneth
Application Number:
JP2001570714A
Publication Date:
September 30, 2003
Filing Date:
March 28, 2001
Export Citation:
Assignee:
Queensland University of Technology
Harding Robert Maxwell
Show pom pan Slimek
Harding Robert Maxwell
Show pom pan Slimek
International Classes:
C12N5/10; C12N15/09; C12N15/79; A01H5/00; C12N15/82; (IPC1-7): C12N15/09; A01H5/00; C12N5/10
Domestic Patent References:
| JPH11506322A | 1999-06-08 |
Other References:
JPN6010055233, Ｊｏｕｒｎａｌ ｏｆ Ｇｅｎｅｒａｌ Ｖｉｒｏｌｏｇｙ, 1997, Ｖｏｌ．７８, ｐ．１７９５−１７９９
JPN6010055234, Ｊｏｕｒｎａｌ ｏｆ Ｇｅｎｅｒａｌ Ｖｉｒｏｌｏｇｙ, 1997, Ｖｏｌ．７８, ｐ．２２９−２３６
JPN6010055236, Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐｏｒｔｓ, 1998, Ｖｏｌ．１７, ｐ．６３１−６３９
JPN6010055234, Ｊｏｕｒｎａｌ ｏｆ Ｇｅｎｅｒａｌ Ｖｉｒｏｌｏｇｙ, 1997, Ｖｏｌ．７８, ｐ．２２９−２３６
JPN6010055236, Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐｏｒｔｓ, 1998, Ｖｏｌ．１７, ｐ．６３１−６３９
Attorney, Agent or Firm:
Shimizu Hatsushi (1 person outside)