Title:
一種の突然変異型のヒト組織型プラスミノゲン活性剤を発現する細胞株、構築方法及び発現蛋白の調剤方法
Document Type and Number:
Japanese Patent JP2004508066
Kind Code:
A
Abstract:
The invention involves a cell line which can express human mutated tissue-type plasminogen activator (TNK-TPA), and its preparation methods. The collection No. of cell line in this invention is CCTCC C200006. We firstly use a DNA fragment from the short arms of human D, G group chromosomes or its homologous to construct a recombinant human source gene vector-TNK-TPA (collection number is CCTC m200032); TNK-TPA gene is then transferred into the target site of nucleolus organizing region (NOR) of human D, G group chromosomes in host cell HT1080 by the recombinant and the cell line is attained after screening, which can be used for manufacturing protein.
Inventors:
Natsuya Kagayaki
Application Number:
JP2002527274A
Publication Date:
March 18, 2004
Filing Date:
February 16, 2001
Export Citation:
Assignee:
Natsuya Kagayaki
International Classes:
A61K38/48; A61P7/02; C07K14/745; C12N5/10; C12N9/72; C12N15/57; C12N15/09; C12P21/02; (IPC1-7): C12N15/09; A61K38/48; A61P7/02; C12N5/10; C12P21/02
Attorney, Agent or Firm:
Takenori Hiroe
Kenichi Uno
Kenichi Uno