Title:
核酸の新規分析方法、および、mRNA、特にセロトニン5-HT2C受容体のmRNAのエディティング度を評価するためのその使用
Document Type and Number:
Japanese Patent JP2005533510
Kind Code:
A
Abstract:
The invention concerns a method for analyzing nucleic acids using a small-size probe array comprising deoxylnostines (dl) instead of deoxyguanosines (dG). The invention also concerns such probe arrays and their use in methods for detecting and/or quantifying target oilgonucleotides present in DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) molecules in a sample, in particular mRNA editing rate of the serotonin 5-HT2c receptor (5-HT2c-R). The invention further concerns a biochip or a reactor in liquid medium comprising such probe arrays as well as their uses, in particular for detecting and/or identifying genetic polymorphisms or for determining an mRNA editing rate, whether it is that of a 5-HT2c-R mRNA or any other RNA capable of being edited. The invention also concerns a method based on the isolation of a single strand conformation polymorphism (SSCP) enabling under specific analysis conditions the editing profile and/or rate of an mRNA capable of being edited to be obtained, as well as a method for diagnosing diseases or susceptibility to diseases associated with the degree of edition of an mRNA. Finally, the invention concerns a method for selecting compounds capable of modulating mRNA editing rate, in particular that of 5-HT2c-R, as well as the use of such compounds for preparing a pharmaceutical composition for treating organic fluid.
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Inventors:
Jean-Jack, Magyar
Elbe, Bertom
Elbe, Bertom
Application Number:
JP2004523873A
Publication Date:
November 10, 2005
Filing Date:
July 24, 2003
Export Citation:
Assignee:
BIOCORTECH
International Classes:
G01N33/53; C12M1/00; C12M1/34; C12N15/09; C12Q1/02; C12Q1/68; G01N37/00; (IPC1-7): C12N15/09; C12M1/00; C12M1/34; C12Q1/02; C12Q1/68; G01N33/53; G01N37/00
Foreign References:
| WO1993014217A1 | 1993-07-22 | |||
| WO2002038809A1 | 2002-05-16 |
Other References:
JPN6013008729; Ｎｅｕｒｏｐｓｙｃｈｏｐｈａｒｍａｃｏｌｏｇｙ Ｖｏｌ．２４，Ｎｏ．５, 200105, ４７８-４９１
JPN6013008730; Ｍｏｌ．Ｐｓｙｃｈｉａｔｒｙ Ｖｏｌ．６，Ｎｏ．４, 200107, ３７３-３７９
JPN6013008731; 中山広樹: バイオ実験イラストレイテッド３新版 本当にふえるＰＣＲ 第２版第２刷, 19970701, ８５-８７，１３３-１３８, 株式会社秀潤社
JPN6013008732; Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ．Ｒｅｓ．Ｃｏｍｍｕｎ． Ｖｏｌ．２２３，Ｎｏ．３, 19960625, ５２０-５２５
JPN6013008733; Ｍｏｌ．Ｐｓｙｃｈｉａｔｒｙ Ｖｏｌ．４，Ｎｏ．４, 199907, ３３９-３４３
JPN6009022262; 'Ａｎｎａｌｓ ｏｆ ｔｈｅ Ｎｅｗ Ｙｏｒｋ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ' １９９８， Ｖｏｌ．８６１， ｐｐ．３８-４８
JPN6009022266; 'Ｍｅｔｈｏｄｓ ｉｎ Ｅｎｚｙｍｏｌｏｇｙ' ２００２， Ｖｏｌ．３４３， ｐｐ．４７６-４９２
JPN6009022270; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' １９８５， Ｖｏｌ．１３， Ｎｏ．２４， ｐｐ．８９２７-８９３８
JPN6009022272; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' １９９４， Ｖｏｌ．２２， Ｎｏ．２， ｐｐ．１３１-１３６
JPN6009022275; 'Ｃｕｒｒｅｎｔ Ｇｅｎｅｔｉｃｓ' ２００１， Ｖｏｌ．３９， ｐｐ．３８４-３８７
JPN6009022277; 'Ｂｉｏｃｈｅｍｉｃａｌ ａｎｄ Ｂｉｏｐｈｙｓｉｃａｌ Ｒｅｓｅａｒｃｈ Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ' １９９６， Ｖｏｌ．２２３， ｐｐ．５２０-５２５
JPN6009022279; 'Ｐｌａｎｔ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ' ２００１， Ｖｏｌ．４５， ｐｐ．４４９-４６０
JPN6013008730; Ｍｏｌ．Ｐｓｙｃｈｉａｔｒｙ Ｖｏｌ．６，Ｎｏ．４, 200107, ３７３-３７９
JPN6013008731; 中山広樹: バイオ実験イラストレイテッド３新版 本当にふえるＰＣＲ 第２版第２刷, 19970701, ８５-８７，１３３-１３８, 株式会社秀潤社
JPN6013008732; Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ．Ｒｅｓ．Ｃｏｍｍｕｎ． Ｖｏｌ．２２３，Ｎｏ．３, 19960625, ５２０-５２５
JPN6013008733; Ｍｏｌ．Ｐｓｙｃｈｉａｔｒｙ Ｖｏｌ．４，Ｎｏ．４, 199907, ３３９-３４３
JPN6009022262; 'Ａｎｎａｌｓ ｏｆ ｔｈｅ Ｎｅｗ Ｙｏｒｋ Ａｃａｄｅｍｙ ｏｆ Ｓｃｉｅｎｃｅｓ' １９９８， Ｖｏｌ．８６１， ｐｐ．３８-４８
JPN6009022266; 'Ｍｅｔｈｏｄｓ ｉｎ Ｅｎｚｙｍｏｌｏｇｙ' ２００２， Ｖｏｌ．３４３， ｐｐ．４７６-４９２
JPN6009022270; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' １９８５， Ｖｏｌ．１３， Ｎｏ．２４， ｐｐ．８９２７-８９３８
JPN6009022272; 'Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ' １９９４， Ｖｏｌ．２２， Ｎｏ．２， ｐｐ．１３１-１３６
JPN6009022275; 'Ｃｕｒｒｅｎｔ Ｇｅｎｅｔｉｃｓ' ２００１， Ｖｏｌ．３９， ｐｐ．３８４-３８７
JPN6009022277; 'Ｂｉｏｃｈｅｍｉｃａｌ ａｎｄ Ｂｉｏｐｈｙｓｉｃａｌ Ｒｅｓｅａｒｃｈ Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ' １９９６， Ｖｏｌ．２２３， ｐｐ．５２０-５２５
JPN6009022279; 'Ｐｌａｎｔ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ' ２００１， Ｖｏｌ．４５， ｐｐ．４４９-４６０
Attorney, Agent or Firm:
Kenji Yoshitake
Yukitaka Nakamura
Konno Akio
Noritaka Yokota
Takeyasu Ito
Yukitaka Nakamura
Konno Akio
Noritaka Yokota
Takeyasu Ito