Title:
集光性多発色団を用いてポリヌクレオチドを検出及び分析するための方法並びに組成物
Document Type and Number:
Japanese Patent JP2006510389
Kind Code:
A
Abstract:
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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Inventors:
Bazan, Guillermo, Si.
Gaylord, Brent, Es.
One, Shu
Gaylord, Brent, Es.
One, Shu
Application Number:
JP2004570933A
Publication Date:
March 30, 2006
Filing Date:
August 26, 2003
Export Citation:
Assignee:
The Regents of The University of California
International Classes:
C12Q1/68; C07H21/00; C07H21/02; C07H21/04; C12N15/09; G01N21/00; G01N21/64; G01N21/78; G01N33/53; G01N33/542; C12N
Other References:
CARDULLO R.A. ET AL: ""Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer."", PROC. NATL. ACAD. SCI. USA, vol. 85, JPN6009011876, 1988, pages 8790 - 8794, XP000453537, ISSN: 0001314569, DOI: 10.1073/pnas.85.23.8790
PNAS,VOL.99(AUG.20,2002)P.10954-10957, JPN6009021473, ISSN: 0001314570
ADV.MATER.,VOL.14,NO.5(MAR.2002)P.361-366, JPN6009021476, ISSN: 0001314571
JACS,VOL.123(2001)P.6417-6418, JPN6009021479, ISSN: 0001575260
PNAS,VOL.99(AUG.20,2002)P.10954-10957, JPN6009021473, ISSN: 0001314570
ADV.MATER.,VOL.14,NO.5(MAR.2002)P.361-366, JPN6009021476, ISSN: 0001314571
JACS,VOL.123(2001)P.6417-6418, JPN6009021479, ISSN: 0001575260
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita