Title:
細胞上および生物学的混合物中の抗原の検出のための組成物、方法およびキット
Document Type and Number:
Japanese Patent JP2006516088
Kind Code:
A
Abstract:
The present invention relates to novel methods for detecting at least one member of a known binding pair in a sample, including a cell, where one member of the pair (termed the "receptor") is expressed by a bacteriophage, which phage is then used to detect the presence of the other member of the pair (termed the "ligand" or "target"). Rather than detecting the binding of at least one phage using antibody-based technology, the present invention relates to detecting the nucleic acid associated with the phages. In one aspect, the invention relates to identifying at least one antigen-bearing moiety (e.g., a red blood cell antigen) of interest present on a cell, e.g., a red blood cell, using antibody-displaying bacteriophages, using antiglobulin reagent-displaying bacteriophages and detecting at least one nucleic acid associated with the phage.
Inventors:
Siegel, Donald El
Application Number:
JP2004537912A
Publication Date:
June 22, 2006
Filing Date:
September 18, 2003
Export Citation:
Assignee:
The Trustees of the University of Pennsylvania
International Classes:
C12Q1/68; C12N7/00; C12N15/09; C12N15/10; C12N15/86; C12P19/34; C12Q1/02; C12Q1/70; C40B40/02; G01N33/53; G01N33/567; G01N33/569; G01N33/80
Foreign References:
| WO1998016827A1 | 1998-04-23 |
Attorney, Agent or Firm:
Heikichi Odashima