Title:
疎水性相互作用クロマトグラフィー用リガンドの製造法
Document Type and Number:
Japanese Patent JP2006523308
Kind Code:
A
Abstract:
The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
Inventors:
Johansson, Bo-Renart
Maroizel, Jean Look
Nicholas Sevenin
Maroizel, Jean Look
Nicholas Sevenin
Application Number:
JP2006507945A
Publication Date:
October 12, 2006
Filing Date:
March 05, 2004
Export Citation:
Assignee:
GE Healthcare Bioscience Activolag
International Classes:
G01N30/88; B01D15/32; B01J20/281; B01J20/286; C07C319/18; C07C323/63; C07D213/40; C07D295/18; C07D307/52; C07D333/20; G01N30/08; B01D15/08
Domestic Patent References:
| JPS59104377A | 1984-06-16 | |||
| JP2000351776A | 2000-12-19 | |||
| JPH04503157A | 1992-06-11 |
Foreign References:
| WO2002053288A2 | 2002-07-11 | |||
| WO2001038227A2 | 2001-05-31 | |||
| WO2003024588A1 | 2003-03-27 | |||
| WO2004076475A1 | 2004-09-10 |
Attorney, Agent or Firm:
Kenichi Matsumoto
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa