Title:
肝細胞成長因子のN末端フラグメントを組み換え発現するための方法
Document Type and Number:
Japanese Patent JP2007525981
Kind Code:
A
Abstract:
A method for the production of the α-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.
Inventors:
Auer, Johannes
Papadimitriou, Apollon
Shunts, christian
Jiber, stephan
Papadimitriou, Apollon
Shunts, christian
Jiber, stephan
Application Number:
JP2007501205A
Publication Date:
September 13, 2007
Filing Date:
March 02, 2005
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12N15/09; C07K14/475; C12N15/70; C12P21/02
Other References:
JPN6009048062; Ｂｉｏｃｈｅｍ． Ｊ． Ｖｏｌ．３２６， Ｐａｒｔ ３, 19970915, ｐｐ．７６３-７７２
JPN6009048065; Ｇｅｎｅ Ｖｏｌ．３２０, 20031127, ｐｐ．１８５-１９０
JPN6009048068; Ｍｏｌ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．４９， Ｎｏ．４, 200308, ｐｐ．１０４３-１０４９
JPN6009048072; Ｊ． Ｂｉｏｔｅｃｈｎｏｌ． Ｖｏｌ．６０， Ｎｏ．３, 19980226, ｐｐ．１８３-１９３
JPN6013041533; Ｍｏｌｅｃｕｌａｒ Ｍｉｃｒｏｂｉｏｌｏｇｙ Ｖｏｌ．２１，Ｎｏ．１, 1996, ｐ．６９-７６
JPN6013041534; Ｂｉｏｃｈｅｍｉｃａｌ ａｎｄ Ｂｉｏｐｈｙｓｉｃａｌ Ｒｅｓｅａｒｃｈ Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ Ｖｏｌ．１６３，Ｎｏ．２, 19890915, ｐ．９６７-９７３
JPN6009048065; Ｇｅｎｅ Ｖｏｌ．３２０, 20031127, ｐｐ．１８５-１９０
JPN6009048068; Ｍｏｌ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ．４９， Ｎｏ．４, 200308, ｐｐ．１０４３-１０４９
JPN6009048072; Ｊ． Ｂｉｏｔｅｃｈｎｏｌ． Ｖｏｌ．６０， Ｎｏ．３, 19980226, ｐｐ．１８３-１９３
JPN6013041533; Ｍｏｌｅｃｕｌａｒ Ｍｉｃｒｏｂｉｏｌｏｇｙ Ｖｏｌ．２１，Ｎｏ．１, 1996, ｐ．６９-７６
JPN6013041534; Ｂｉｏｃｈｅｍｉｃａｌ ａｎｄ Ｂｉｏｐｈｙｓｉｃａｌ Ｒｅｓｅａｒｃｈ Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ Ｖｏｌ．１６３，Ｎｏ．２, 19890915, ｐ．９６７-９７３
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Shintaro Koike
Takashi Ishida
Fukumoto product
Tetsuji Koga
Shintaro Koike