Title:
新規な胚様体(EB)の調製方法およびその使用
Document Type and Number:
Japanese Patent JP2009513107
Kind Code:
A
Abstract:
Provided are means and methods for producing embryoid bodies (EBs) from multi- or pluripotent cells. In particular, a method of generating embryoid bodies (EBs) is described comprising agitation of a liquid suspension culture of multi- or pluripotent cells in a container until generation of cell aggregates, optionally diluting the suspension, and further agitation of the suspension until formation of EBs. Furthermore, the present invention relates to the use of the novel culturing method and EBs obtained thereby for a variety of applications including genomics, diagnostic assays, teratogenic/embryotoxicological and pharmacological assays as well as for the provision of tissue grafts.
Inventors:
Korosov, Jugen
Kettenhofen, Ralph
Cup, isabella
Bohren, Helibelt
Schwenberg, Silke
Kettenhofen, Ralph
Cup, isabella
Bohren, Helibelt
Schwenberg, Silke
Application Number:
JP2006518153A
Publication Date:
April 02, 2009
Filing Date:
July 08, 2004
Export Citation:
Assignee:
AXIOGENESIS AG
International Classes:
A61K35/48; A61L27/00; A61P9/00; A61P9/04; C12N5/073; C12N5/077; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50
Foreign References:
| US20030119107A1 | 2003-06-26 | |||
| WO2001062899A2 | 2001-08-30 | |||
| US6190913B1 | 2001-02-20 |
Attorney, Agent or Firm:
Tomimasa Konishi
Tomoko Nakamura
Mikiharu Hagino
Tomoko Nakamura
Mikiharu Hagino