Title:
接着結合を持つ細胞の増殖を刺激するRNAi方法および組成物
Document Type and Number:
Japanese Patent JP2010505001
Kind Code:
A
Abstract:
Described herein are methods and compositions for stimulating proliferation of cells that express adherent junctions and cease proliferation, for example, human corneal endothelial cells, by downregulation of certain cell-cell junctions. In one embodiment, downregulation is achieved using RNA interference, and contacting the cells with mitogenic growth factors and an agent that elevates intracytoplasmic cAMP. Furthermore, described herein are methods of isolating human corneal endothelial cells from keratocytes, and methods of preserving and maintaining viability of human corneal endothelial cell aggregates. Also described are surgical grafts comprising human corneal endothelial cells that have been isolated, optionally stored, and transiently contacted with an agent that downregulates expression of p 120, and a biocompatible support. The methods and compositions described herein can be used in novel therapies to help expand human corneal endothelial cells during in vitro tissue engineering and for in vivo treatment of corneal endothelial dysfunction.
Inventors:
Sheffer Zen
Re-way
Intin Chu
Re-way
Intin Chu
Application Number:
JP2009530605A
Publication Date:
February 18, 2010
Filing Date:
September 27, 2007
Export Citation:
Assignee:
TISSUETECH, INC.
International Classes:
A61K31/713; A61K35/44; A61K38/16; A61K45/00; A61L27/00; A61P27/02; A61P43/00; C12N5/07; C12N5/071; C12N15/113; C12N15/09
Other References:
JPN6012038918; ＴＨＥ ＪＯＵＲＮＡＬ ＯＦ ＣＥＬＬ ＢＩＯＬＯＧＹ ｖｏｌ．１７３， ｎｏ．３, 200605, ｐ．４３１-４４１
JPN6013015094; Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｃｅｌｌ Ｂｉｏｌｏｇｙ ｖｏｌ．１６９， ｎｏ．１, 2005, ｐ．２９-３４
JPN6013015094; Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｃｅｌｌ Ｂｉｏｌｏｇｙ ｖｏｌ．１６９， ｎｏ．１, 2005, ｐ．２９-３４
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Masaki Yano
Tsuyoshi Sato
Hiroshi Yamazaki
Masaki Yano
Tsuyoshi Sato