Title:
尿および組換えチャイニーズハムスター卵巣(CHO)細胞から濃縮された好中球ゼラチナーゼ関連リポカリン(NGAL)タンパク質アイソフォーム、ならびに関連する組成物、抗体、ならびに濃縮、分析および使用の方法
Document Type and Number:
Japanese Patent JP2012508177
Kind Code:
A
Abstract:
A composition comprising neutrophil gelatinase-associated lipocalin (NGAL), which has been enriched from urine, has a molecular weight of about 24.9 kDa to about 25.9 kDa, and comprises a plurality of isoforms having isoelectric points (pIs) ranging from about 5.9 to about 9.1; a composition comprising NGAL, which has been enriched from recombinant Chinese hamster ovary (CHO) cells, has a molecular weight of about 25.9 kDa to about 27.9 kDa, and comprises a plurality of isoforms having pIs ranging from about 5.6 to about 9.1; a method of obtaining from urine a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in urine without separating molecules based on charge; a method of obtaining from recombinant CHO cells a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in a composition without separating molecules based on charge; and a method of analyzing NGAL isoforms enriched from urine or recombinant CHO cells comprising analyzing an enriched composition comprising NGAL isoforms by two-dimensional electrophoresis and Western blot.
Inventors:
Ant salman
Bonn, Ryan Em
Frank Sea, Glenier
Ray, Tracy Day
Ruprecht, Kevin Earl
Hina N, Ciedo
Tow, bilin
Tyner, Joon Day
Workman, Ryan Eff
Bonn, Ryan Em
Frank Sea, Glenier
Ray, Tracy Day
Ruprecht, Kevin Earl
Hina N, Ciedo
Tow, bilin
Tyner, Joon Day
Workman, Ryan Eff
Application Number:
JP2011534909A
Publication Date:
April 05, 2012
Filing Date:
November 04, 2009
Export Citation:
Assignee:
ABBOTT LABORATORIES
International Classes:
C07K14/47; C12P21/02; G01N27/447; G01N33/53
Domestic Patent References:
| JP2008528006A | 2008-07-31 | |||
| JP2007527699A | 2007-10-04 |
Foreign References:
| WO2007044994A2 | 2007-04-19 | |||
| US6528286B1 | 2003-03-04 |
Other References:
GRENIER F: "MULTI-SITE EVALUATION OF AN ASSAY IN DEVELOPMENT FOR NGAL 以下備考", CLINICAL CHEMISTRY, vol. V54 N6, JPN5012002848, June 2008 (2008-06-01), pages 170, ISSN: 0002744901
BIOCHEMISTRY, vol. 38, no. 42, JPN6014005894, 1999, pages 13937 - 13950, ISSN: 0002744902
BUNDGAARD J R: "MOLECULAR CLONING AND EXPRESSION OF A CDNA ENCODING NGAL: A LIPOCALIN EXPRESSED IN HUMAN NEUTROPHILS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. V202 N3, JPN5012002851, 15 August 1994 (1994-08-15), US, pages 1468 - 1475, ISSN: 0002744903
ARCHIVES OF MEDICAL RESEARCH, vol. 39, JPN6014005895, August 2008 (2008-08-01), pages 560 - 566, ISSN: 0002744904
BIOCHEMISTRY, vol. 38, no. 42, JPN6014005894, 1999, pages 13937 - 13950, ISSN: 0002744902
BUNDGAARD J R: "MOLECULAR CLONING AND EXPRESSION OF A CDNA ENCODING NGAL: A LIPOCALIN EXPRESSED IN HUMAN NEUTROPHILS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. V202 N3, JPN5012002851, 15 August 1994 (1994-08-15), US, pages 1468 - 1475, ISSN: 0002744903
ARCHIVES OF MEDICAL RESEARCH, vol. 39, JPN6014005895, August 2008 (2008-08-01), pages 560 - 566, ISSN: 0002744904
Attorney, Agent or Firm:
Kawaguchi International Patent Office