Title:
引き抜かれた毛包由来のケラチン生成細胞から誘導多能性幹細胞を生成させる方法
Document Type and Number:
Japanese Patent JP2013535966
Kind Code:
A
Abstract:
A method for generating induced pluripotent stem (iPS) cells from isolated hair follicles is disclosed. The method comprises: a. culturing isolated hair follicle keratinocytes on a layer of feeder cells, so as to generate colonies of hair follicle keratinocytes; b. detaching the colonies of hair follicle keratinocytes from the feeder cells so as to generate detached keratinocytes; c. infecting the detached keratinocytes with a virus comprising a nucleic acid molecule encoding at least one dedifferentiation factor so as to generated infected keratinocytes; and d. culturing the infected keratinocytes on a layer of feeder cells in a culture medium until iPS cells are formed, thereby generating iPS cells. Populations and uses of the iPS cells are also disclosed.
Inventors:
Itskowitz-Erdeau, Joseph
Novak-Petralo, Atara
Stritchman, Ronnit
Novak-Petralo, Atara
Stritchman, Ronnit
Application Number:
JP2013521283A
Publication Date:
September 19, 2013
Filing Date:
July 27, 2011
Export Citation:
Assignee:
TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.
International Classes:
C12N5/10; A61P37/00; C12N5/071; A61K35/36; A61P1/16; A61P3/10; A61P9/10; A61P25/16; C12N15/09
Foreign References:
| WO2010077955A1 | 2010-07-08 | |||
| WO2010042490A1 | 2010-04-15 |
Other References:
JPN6015029154; 'Ｊｏｕｒｎａｌ ｏｆ Ｉｎｖｅｓｔｉｇａｔｉｖｅ Ｄｅｒｍａｔｏｌｏｇｙ' １９８６， Ｖｏｌ．８７， ｐｐ．４８５-４８８
JPN5013009049; ＭＡＲＩＵＳ ＷＥＲＮＩＧ: 'Ａ ＤＲＵＧ-ＩＮＤＵＣＩＢＬＥ ＴＲＡＮＳＧＥＮＩＣ ＳＹＳＴＥＭ ＦＯＲ ＤＩＲＥＣＴ ＲＥＰＲＯＧＲＡＭＭＩＮＧ ＯＦ ＭＵＬＴＩＰＬＥ ＳＯＭＡＴＩＣ ＣＥＬＬ ＴＹＰＥＳ' ＮＡＴＵＲＥ ＢＩＯＴＥＣＨＮＯＬＯＧＹ Ｖ２６ Ｎ８, 20080801, Ｐ９１６-９２４, ＮＡＴＵＲＥ ＰＵＢＬＩＳＨＩＮＧ ＧＲＯＵＰ
JPN6015029155; 'ＰＬｏＳ Ｂｉｏｌｏｇｙ' ２００８， Ｖｏｌ．６， Ｎｏ．１０， ｅ２５３， ｐｐ．２２３７-２２４７
JPN5013009050; ＴＲＯＮＤ ＡＡＳＥＮ: 'ＥＦＦＩＣＩＥＮＴ ＡＮＤ ＲＡＰＩＤ ＧＥＮＥＲＡＴＩＯＮ ＯＦ ＩＮＤＵＣＥＤ ＰＬＵＲＩＰＯＴＥＮＴ ＳＴＥＭ ＣＥＬＬＳ ＦＲＯＭ ＨＵＭＡＮ ＫＥＲＡＴＩＮＯＣＹＴＥＳ' ＮＡＴＵＲＥ ＢＩＯＴＥＣＨＮＯＬＯＧＹ Ｖ２６ Ｎ１１, 20081101, Ｐ１２７６-１２８４
JPN5013009048; ＷＥＮＬＩＮ ＬＩ: 'ＧＥＮＥＲＡＴＩＯＮ ＯＦ ＨＵＭＡＮ-ＩＮＤＵＣＥＤ ＰＬＵＲＩＰＯＴＥＮＴ ＳＴＥＭ ＣＥＬＬＳ ＩＮ ＴＨＥ ＡＢＳＥＮＣＥ ＯＦ ＥＸＯＧＥＮＯＵＳ ＳＯＸ２' ＳＴＥＭ ＣＥＬＬＳ , 20090101, Ｐ２９９２-３０００
JPN5013009051; ＡＡＳＥＮ ＴＲＯＮＤ: 'ＩＳＯＬＡＴＩＯＮ ＡＮＤ ＣＵＬＴＩＶＡＴＩＯＮ ＯＦ ＨＵＭＡＮ ＫＥＲＡＴＩＮＯＣＹＴＥＳ ＦＲＯＭ ＳＫＩＮ ＯＲ ＰＬＵＣＫＥＤ ＨＡＩＲ 以下備考' ＮＡＴＵＲＥ ＰＲＯＴＯＣＯＬＳ Ｖ５ Ｎ２, 20100101, Ｐ３７１-３８２, ＮＡＴＵＲＥ ＰＵＢＬＩＳＨＩＮＧ ＧＲＯＵＰ
JPN5013009052; ＹＡＭＡＳＨＩＴＡ: 'ＥＳ ＡＮＤ ＩＰＳ ＣＥＬＬ ＲＥＳＥＡＲＣＨ ＦＯＲ ＣＡＲＤＩＯＶＡＳＣＵＬＡＲ ＲＥＧＥＮＥＲＡＴＩＯＮ' ＥＸＰＥＲＩＭＥＮＴＡＬ ＣＥＬＬ ＲＥＳＥＡＲＣＨ Ｖ３１６ Ｎ１６, 20100410, Ｐ２５５５-２５５９, ＡＣＡＤＥＭＩＣ ＰＲＥＳＳ
JPN5013009049; ＭＡＲＩＵＳ ＷＥＲＮＩＧ: 'Ａ ＤＲＵＧ-ＩＮＤＵＣＩＢＬＥ ＴＲＡＮＳＧＥＮＩＣ ＳＹＳＴＥＭ ＦＯＲ ＤＩＲＥＣＴ ＲＥＰＲＯＧＲＡＭＭＩＮＧ ＯＦ ＭＵＬＴＩＰＬＥ ＳＯＭＡＴＩＣ ＣＥＬＬ ＴＹＰＥＳ' ＮＡＴＵＲＥ ＢＩＯＴＥＣＨＮＯＬＯＧＹ Ｖ２６ Ｎ８, 20080801, Ｐ９１６-９２４, ＮＡＴＵＲＥ ＰＵＢＬＩＳＨＩＮＧ ＧＲＯＵＰ
JPN6015029155; 'ＰＬｏＳ Ｂｉｏｌｏｇｙ' ２００８， Ｖｏｌ．６， Ｎｏ．１０， ｅ２５３， ｐｐ．２２３７-２２４７
JPN5013009050; ＴＲＯＮＤ ＡＡＳＥＮ: 'ＥＦＦＩＣＩＥＮＴ ＡＮＤ ＲＡＰＩＤ ＧＥＮＥＲＡＴＩＯＮ ＯＦ ＩＮＤＵＣＥＤ ＰＬＵＲＩＰＯＴＥＮＴ ＳＴＥＭ ＣＥＬＬＳ ＦＲＯＭ ＨＵＭＡＮ ＫＥＲＡＴＩＮＯＣＹＴＥＳ' ＮＡＴＵＲＥ ＢＩＯＴＥＣＨＮＯＬＯＧＹ Ｖ２６ Ｎ１１, 20081101, Ｐ１２７６-１２８４
JPN5013009048; ＷＥＮＬＩＮ ＬＩ: 'ＧＥＮＥＲＡＴＩＯＮ ＯＦ ＨＵＭＡＮ-ＩＮＤＵＣＥＤ ＰＬＵＲＩＰＯＴＥＮＴ ＳＴＥＭ ＣＥＬＬＳ ＩＮ ＴＨＥ ＡＢＳＥＮＣＥ ＯＦ ＥＸＯＧＥＮＯＵＳ ＳＯＸ２' ＳＴＥＭ ＣＥＬＬＳ , 20090101, Ｐ２９９２-３０００
JPN5013009051; ＡＡＳＥＮ ＴＲＯＮＤ: 'ＩＳＯＬＡＴＩＯＮ ＡＮＤ ＣＵＬＴＩＶＡＴＩＯＮ ＯＦ ＨＵＭＡＮ ＫＥＲＡＴＩＮＯＣＹＴＥＳ ＦＲＯＭ ＳＫＩＮ ＯＲ ＰＬＵＣＫＥＤ ＨＡＩＲ 以下備考' ＮＡＴＵＲＥ ＰＲＯＴＯＣＯＬＳ Ｖ５ Ｎ２, 20100101, Ｐ３７１-３８２, ＮＡＴＵＲＥ ＰＵＢＬＩＳＨＩＮＧ ＧＲＯＵＰ
JPN5013009052; ＹＡＭＡＳＨＩＴＡ: 'ＥＳ ＡＮＤ ＩＰＳ ＣＥＬＬ ＲＥＳＥＡＲＣＨ ＦＯＲ ＣＡＲＤＩＯＶＡＳＣＵＬＡＲ ＲＥＧＥＮＥＲＡＴＩＯＮ' ＥＸＰＥＲＩＭＥＮＴＡＬ ＣＥＬＬ ＲＥＳＥＡＲＣＨ Ｖ３１６ Ｎ１６, 20100410, Ｐ２５５５-２５５９, ＡＣＡＤＥＭＩＣ ＰＲＥＳＳ
Attorney, Agent or Firm:
Nobuaki Kazehaya
Noriko Asano
Noriko Asano