Title:
効率的な人工多能性幹細胞の樹立方法
Document Type and Number:
Japanese Patent JP2013537035
Kind Code:
A
Abstract:
The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells by inhibiting p38 function in the step of somatic cell nuclear reprogramming. The 38 function can be inhibited by bringing an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway into contact with a somatic cell and the like. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cells, which contains an inhibitor of p38 function, particularly an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway. Moreover, the present invention provides a production method of iPS cells, which includes bringing a nuclear reprogramming substance and an inhibitor of p38 function into contact with a somatic cell.
Inventors:
Shinya Yamanaka
Yoshinori Yoshida
Yokota Hidaka
Yoshinori Yoshida
Yokota Hidaka
Application Number:
JP2013512032A
Publication Date:
September 30, 2013
Filing Date:
September 14, 2011
Export Citation:
Assignee:
Kyoto University
International Classes:
C12N5/10; C12N15/00; C12N15/09
Foreign References:
| WO2009157593A1 | 2009-12-30 | |||
| WO2010121465A1 | 2010-10-28 | |||
| WO2010077955A1 | 2010-07-08 | |||
| WO2009117439A2 | 2009-09-24 |
Other References:
HONG, H., ET AL.: "Suppression of induced pluripotent stem cell generation by the p53-p21 pathway", NATURE, vol. 460(7259), JPN6015028685, 27 August 2009 (2009-08-27), pages 1132 - 1135, ISSN: 0003117153
ZHAO, Y., ET AL.: "Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation", CELL STEM CELL, vol. 3(5), JPN6015028688, 6 November 2008 (2008-11-06), pages 475 - 479, ISSN: 0003117154
LIN, T., ET AL.: "A chemical platform for improved induction of human iPSCs", NATURE METHODS, vol. 6(11), JPN7015001980, November 2009 (2009-11-01), pages 805 - 808, ISSN: 0003117155
SILVA, J., ET AL.: "Promotion of reprogramming to ground state pluripotency by signal inhibition", PLOS BIOL., vol. 6(10), JPN6015028689, 21 October 2008 (2008-10-21), pages 253, ISSN: 0003117156
SHI, Y., ET AL.: "A Combined Chemical and Genetic Approach for the Generation of Induced Pluripotent Stem Cells", CELL STEM CELL, vol. 2(6), JPN6015028691, 5 June 2008 (2008-06-05), pages 525 - 528, ISSN: 0003117157
HUANGFU, D., ET AL.: "Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compoun", NATURE BIOTECHNOLOGY, vol. 26(7), JPN6015028693, July 2008 (2008-07-01), pages 795 - 797, ISSN: 0003117158
ZHAO, Y., ET AL.: "Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation", CELL STEM CELL, vol. 3(5), JPN6015028688, 6 November 2008 (2008-11-06), pages 475 - 479, ISSN: 0003117154
LIN, T., ET AL.: "A chemical platform for improved induction of human iPSCs", NATURE METHODS, vol. 6(11), JPN7015001980, November 2009 (2009-11-01), pages 805 - 808, ISSN: 0003117155
SILVA, J., ET AL.: "Promotion of reprogramming to ground state pluripotency by signal inhibition", PLOS BIOL., vol. 6(10), JPN6015028689, 21 October 2008 (2008-10-21), pages 253, ISSN: 0003117156
SHI, Y., ET AL.: "A Combined Chemical and Genetic Approach for the Generation of Induced Pluripotent Stem Cells", CELL STEM CELL, vol. 2(6), JPN6015028691, 5 June 2008 (2008-06-05), pages 525 - 528, ISSN: 0003117157
HUANGFU, D., ET AL.: "Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compoun", NATURE BIOTECHNOLOGY, vol. 26(7), JPN6015028693, July 2008 (2008-07-01), pages 795 - 797, ISSN: 0003117158
Attorney, Agent or Firm:
Takashima Ichi
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Kenji Yamamoto
Murata Miyuki
Junzo Koike
Hirofumi Toma
Kyoko Doi
Mitsunori Kamata
Yaeko Tamura
Kenji Yamamoto
Murata Miyuki
Junzo Koike
Hirofumi Toma