Title:
新規組換え抗ＴＮＦ−αキメラモノクローナル抗体の製造方法及び用途
Document Type and Number:
Japanese Patent JP2018502603
Kind Code:
A
Abstract:
組換え抗ＴＮＦ−αキメラモノクローナル抗体の製造方法及び用途を提供する。ハムスターに最適化したコドンに基づいてＣＭＡＢ００８抗体の軽鎖と重鎖を設計・合成し、真核発現ベクターを構築し、ＧＳ遺伝子がノックアウトされたＣＨＯ−ＣＲ−ＧＳ-/-宿主細胞にトランスフェクトし、且つ無血清培養技術で培養し、分離精製し、低免疫原性のＣＭＡＢ００８抗体を得る。
Inventors:
Money
Application Number:
JP2017554632A
Publication Date:
February 01, 2018
Filing Date:
January 04, 2016
Export Citation:
Assignee:
Shanghai Bio-Mabs Pharmaceuticals Company Limited
International Classes:
C12N15/09; A61K31/519; A61K39/395; A61K45/00; A61P17/16; A61P19/02; A61P25/00; A61P29/00; A61P43/00; C12P21/08
Domestic Patent References:
| JP2013510574A | 2013-03-28 | |||
| JP2013532954A | 2013-08-22 |
Foreign References:
| US20060018903A1 | 2006-01-26 | |||
| WO2014058389A1 | 2014-04-17 | |||
| WO2014134657A1 | 2014-09-12 |
Other References:
LIANCHUN FAN ET AL.: "Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter", JOURNAL OF BIOTECHNOLOGY, vol. Vol.168, JPN6018022450, 29 August 2013 (2013-08-29), pages 652 - 658, XP028789036, DOI: doi:10.1016/j.jbiotec.2013.08.021
LIANCHUN FAN ET AL.: "Improving the Efficiency of CHO Cell Line Generation Using Glutamine Synthetase Gene Knockout Cells", BOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 4, JPN6018022452, April 2012 (2012-04-01), pages 1007 - 1015, XP055213290, DOI: doi:10.1002/bit.24365
LIANCHUN FAN ET AL.: "Improving the Efficiency of CHO Cell Line Generation Using Glutamine Synthetase Gene Knockout Cells", BOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 4, JPN6018022452, April 2012 (2012-04-01), pages 1007 - 1015, XP055213290, DOI: doi:10.1002/bit.24365
Attorney, Agent or Firm:
Patent corporation ssinpat