Title:
【発明の名称】インビトロでのDNAの増幅、ゲノムクローニングおよびマッピングの、改良された方法
Document Type and Number:
Japanese Patent JP3030417
Kind Code:
B2
Abstract:
A method for cloning genomic DNA comprises the steps of: (a) digesting genomic DNA with a restriction enzyme that generates multiple different cohesive-end sequences; (b) contacting the digested genomic DNA with a linker, said linker comprising a reporter nucleotide sequence and a cohesive-end sequence complementary to a cohesive-end sequence generated by the restriction enzyme of step (a) under conditions such that a linker is joined to a restriction fragment of genomic DNA; and (c) amplifying the product of step (b). Likewise, a method for mapping genomic DNA restriction fragments comprises the steps of: (a) digesting genomic DNA with a restriction enzyme that generates multiple different cohesive-end sequences; (b) amplifying the restriction fragment products of step (a); (c) determining the cohesive-end sequence of each of said restriction fragments; and in accordance therewith, (d) aligning the restriction fragments in a physical map.
Inventors:
Mirror, harvey eye
Application Number:
JP50439290A
Publication Date:
April 10, 2000
Filing Date:
February 15, 1990
Export Citation:
Assignee:
Genentech, Inc.
International Classes:
C07K14/075; C12N15/10; C12N15/09; C12Q1/68; C12Q1/683; C12Q1/6841; C12Q1/6853; C12Q1/6858; C12Q1/6867; C12Q1/70; (IPC1-7): C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Aoyama Ryo (2 outside people)